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The map label for this gene is spoT [H]

Identifier: 30995477

GI number: 30995477

Start: 1826640

End: 1828754

Strand: Reverse

Name: spoT [H]

Synonym: HI1741

Alternate gene names: 30995477

Gene position: 1828754-1826640 (Counterclockwise)

Preceding gene: 16273628

Following gene: 16273626

Centisome position: 99.92

GC content: 40.19

Gene sequence:

>2115_bases
TTGGAACTGTTTGCGGATTTAGATCGAATTATTCAAGGGTATTTGCCCGCAGATAAAATTGAACTCATCAAGCGTGCGTT
TGTGATTGCAAGAGATGCGCACGAAGGACAATTTCGCTCCAGCGGCGAACCTTACATTACCCATCCTGTGGCGGTAGCCT
CCATTATTGCTCAACTTCACTTAGATCACGAAGCGGTAATGGCTGCGCTTTTGCACGATGTTATTGAAGATACACCTTAT
ACGGAAGAACAACTGAAAGAAGAGTTCGGTGCCAGTGTGGCTGAAATTGTGGACGGTGTATCAAAACTCGATAAGTTGAA
ATTTCGTACTCGCCAAGAAGCACAAGTCGAGAATTTTCGCAAAATGATTTTGGCGATGACGCGTGATATTCGCGTTGTGT
TAATCAAGCTTGCTGACCGCACGCATAATATGCGCACGCTTGGTTCGTTACGACCAGATAAACGTCGCCGTATTGCGAAA
GAAACGCTTGAAATCTATTGTCCGCTTGCTCATCGTTTAGGTATTGAGCATATCAAAAATGAATTAGAAGACTTATCTTT
TCAAGCAATGCATCCACATCGTTATGAGGTGTTAAAAAAACTCGTTGATGTGGCGCGGAGTAATCGACAAGATTTAATCG
AACGTATTTCACAAGAGATTAAAGTGCGGTTAGAAAACTCAGGCATTTTTGCCCGAGTGTGGGGACGTGAAAAACATCTT
TACAAGATCTATCAAAAAATGCGCATAAAGGATCAAGAATTCCACTCTATTATGGATATTTATGCGTTCCGTGTGATTGT
GAAAAATGTTGATGATTGTTATCGCGTTTTGGGGCAAATGCATAATCTTTATAAGCCACGTCCAGGCCGAGTTAAGGATT
ATATCGCGGTACCTAAAGCAAATGGCTATCAATCTTTGCAAACTTCAATGATTGGGCCGAAAGGTGTTCCCGTAGAAGTT
CATATCCATACTGAGGATATGGAACAAGTCGCTGAAATGGGCATTACTGCGCATTGGGTTTATAAAGAAAATGGTAAAAA
CGACAGTACAACCGCACAAATCCGAGTACAACGCTGGTTGCAAAGTTTGGTTGAAATTCAACAAAGTGTAGGTAATTCGT
TTGAGTTTATTGAGAATGTAAAATCAGAGTTTTTCCCGAAAGAAATTTATGTTTTTACGCCAAAAGGTCGAATTGTTGAA
TTGCCAATGGGCGCAACAGCAGTAGATTTTGCTTATGCTGTGCATTCAGATGTGGGGAATACTTGTGTTGGCGTAACCGT
TGAGCATAAACCTTATCCACTTTCAAAAGCCCTTGAATCAGGGCAAACAGTCAATATCATTACTGATCCAAATGCGCATC
CTGAAGTCGCATGGTTAAATTTTGTGGTAACGGCTCGTGCGAAAACACGGATTCGCCATTATCTCAAACAGCGTTGTGAA
GAGGATGCGGTGAAATTAGGCGAAGTTGAGCTTAATGTAGCATTACAGCCGCATAATTTAGGTGATTTTTCTATTCAACA
AATTAGAACTGTATTAGATGCTTTAGCCCTTTCTTCTTTAGATGAATTATTACGAGAAATTGGTTTAGGTAATCAGTCAG
CCTCCATGATTGCGCATCAATTTGTGGGCGTGCCGTTAGAATCTGCTAATACTAAGAATCTCGAATTTGAATCAAAAATA
CTCACTATTGCACCAATGCAAGTGGGTAAAACACAATTTGCTCAATGTTGCCATCCAATCCTAGGTGATCCTATTGTGGG
ATGTTGTACAGAAAAAAATACAGTTGTGGTACACCATCAACATTGTGCAAGTTTGAAAAATGCTTGTCGTCAATCGTTGG
CTAAGTGGGATAACGTTCAAAGTGCGGTGAATTTTGAGGCTGAATTACAAATAGAAATATTGAATGAGCAGAATGCCTTG
CTTAGTTTAATGACCGCAATTTCAGCGAGCGAAAGCAGTCTCCAAAATATTTGGACGGAAGAATTAGAGAACAATTTATT
GCTTGTGATTTTGCAGGTTTGTGTAAAAGATATCAAACACCTTGCCAATATCGTTCATCGTATTAAAGGCATCACTGGCG
TCGTTAATGTCAAACGCAATATTAATGAGCTATGA

Upstream 100 bases:

>100_bases
CCCCTTTGGGGAACGTAATTTCTACCCCAAAGGGTAACATTTAGTTAAGCTAGGGTCTACTTACCCTAGGCGATATTTTT
CAACGATAAGTAGGTCTCCT

Downstream 100 bases:

>100_bases
GCCTTGAACTTCTCGATGCCGTGCCTTTGACCAGCCTTTCTGGTGTGGGGGCTGCCATTTCTAATAAACTGGCTAAAATC
GGCATTCATAATCTGCAAGA

Product: guanosine-3'5'-bis(diphosphate) 3'- pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 704; Mature: 704

Protein sequence:

>704_residues
MELFADLDRIIQGYLPADKIELIKRAFVIARDAHEGQFRSSGEPYITHPVAVASIIAQLHLDHEAVMAALLHDVIEDTPY
TEEQLKEEFGASVAEIVDGVSKLDKLKFRTRQEAQVENFRKMILAMTRDIRVVLIKLADRTHNMRTLGSLRPDKRRRIAK
ETLEIYCPLAHRLGIEHIKNELEDLSFQAMHPHRYEVLKKLVDVARSNRQDLIERISQEIKVRLENSGIFARVWGREKHL
YKIYQKMRIKDQEFHSIMDIYAFRVIVKNVDDCYRVLGQMHNLYKPRPGRVKDYIAVPKANGYQSLQTSMIGPKGVPVEV
HIHTEDMEQVAEMGITAHWVYKENGKNDSTTAQIRVQRWLQSLVEIQQSVGNSFEFIENVKSEFFPKEIYVFTPKGRIVE
LPMGATAVDFAYAVHSDVGNTCVGVTVEHKPYPLSKALESGQTVNIITDPNAHPEVAWLNFVVTARAKTRIRHYLKQRCE
EDAVKLGEVELNVALQPHNLGDFSIQQIRTVLDALALSSLDELLREIGLGNQSASMIAHQFVGVPLESANTKNLEFESKI
LTIAPMQVGKTQFAQCCHPILGDPIVGCCTEKNTVVVHHQHCASLKNACRQSLAKWDNVQSAVNFEAELQIEILNEQNAL
LSLMTAISASESSLQNIWTEELENNLLLVILQVCVKDIKHLANIVHRIKGITGVVNVKRNINEL

Sequences:

>Translated_704_residues
MELFADLDRIIQGYLPADKIELIKRAFVIARDAHEGQFRSSGEPYITHPVAVASIIAQLHLDHEAVMAALLHDVIEDTPY
TEEQLKEEFGASVAEIVDGVSKLDKLKFRTRQEAQVENFRKMILAMTRDIRVVLIKLADRTHNMRTLGSLRPDKRRRIAK
ETLEIYCPLAHRLGIEHIKNELEDLSFQAMHPHRYEVLKKLVDVARSNRQDLIERISQEIKVRLENSGIFARVWGREKHL
YKIYQKMRIKDQEFHSIMDIYAFRVIVKNVDDCYRVLGQMHNLYKPRPGRVKDYIAVPKANGYQSLQTSMIGPKGVPVEV
HIHTEDMEQVAEMGITAHWVYKENGKNDSTTAQIRVQRWLQSLVEIQQSVGNSFEFIENVKSEFFPKEIYVFTPKGRIVE
LPMGATAVDFAYAVHSDVGNTCVGVTVEHKPYPLSKALESGQTVNIITDPNAHPEVAWLNFVVTARAKTRIRHYLKQRCE
EDAVKLGEVELNVALQPHNLGDFSIQQIRTVLDALALSSLDELLREIGLGNQSASMIAHQFVGVPLESANTKNLEFESKI
LTIAPMQVGKTQFAQCCHPILGDPIVGCCTEKNTVVVHHQHCASLKNACRQSLAKWDNVQSAVNFEAELQIEILNEQNAL
LSLMTAISASESSLQNIWTEELENNLLLVILQVCVKDIKHLANIVHRIKGITGVVNVKRNINEL
>Mature_704_residues
MELFADLDRIIQGYLPADKIELIKRAFVIARDAHEGQFRSSGEPYITHPVAVASIIAQLHLDHEAVMAALLHDVIEDTPY
TEEQLKEEFGASVAEIVDGVSKLDKLKFRTRQEAQVENFRKMILAMTRDIRVVLIKLADRTHNMRTLGSLRPDKRRRIAK
ETLEIYCPLAHRLGIEHIKNELEDLSFQAMHPHRYEVLKKLVDVARSNRQDLIERISQEIKVRLENSGIFARVWGREKHL
YKIYQKMRIKDQEFHSIMDIYAFRVIVKNVDDCYRVLGQMHNLYKPRPGRVKDYIAVPKANGYQSLQTSMIGPKGVPVEV
HIHTEDMEQVAEMGITAHWVYKENGKNDSTTAQIRVQRWLQSLVEIQQSVGNSFEFIENVKSEFFPKEIYVFTPKGRIVE
LPMGATAVDFAYAVHSDVGNTCVGVTVEHKPYPLSKALESGQTVNIITDPNAHPEVAWLNFVVTARAKTRIRHYLKQRCE
EDAVKLGEVELNVALQPHNLGDFSIQQIRTVLDALALSSLDELLREIGLGNQSASMIAHQFVGVPLESANTKNLEFESKI
LTIAPMQVGKTQFAQCCHPILGDPIVGCCTEKNTVVVHHQHCASLKNACRQSLAKWDNVQSAVNFEAELQIEILNEQNAL
LSLMTAISASESSLQNIWTEELENNLLLVILQVCVKDIKHLANIVHRIKGITGVVNVKRNINEL

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=711, Percent_Identity=57.1026722925457, Blast_Score=834, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=686, Percent_Identity=31.4868804664723, Blast_Score=352, Evalue=6e-98,
Organism=Caenorhabditis elegans, GI25143118, Length=142, Percent_Identity=33.8028169014084, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 79857; Mature: 79857

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELFADLDRIIQGYLPADKIELIKRAFVIARDAHEGQFRSSGEPYITHPVAVASIIAQLH
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECHHHHHHHHHHHH
LDHEAVMAALLHDVIEDTPYTEEQLKEEFGASVAEIVDGVSKLDKLKFRTRQEAQVENFR
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMILAMTRDIRVVLIKLADRTHNMRTLGSLRPDKRRRIAKETLEIYCPLAHRLGIEHIKN
HHHHHHHCCHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHCCHHHHCCHHHHHH
ELEDLSFQAMHPHRYEVLKKLVDVARSNRQDLIERISQEIKVRLENSGIFARVWGREKHL
HHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEECCCCEEEEECCCHHHH
YKIYQKMRIKDQEFHSIMDIYAFRVIVKNVDDCYRVLGQMHNLYKPRPGRVKDYIAVPKA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
NGYQSLQTSMIGPKGVPVEVHIHTEDMEQVAEMGITAHWVYKENGKNDSTTAQIRVQRWL
CCHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHH
QSLVEIQQSVGNSFEFIENVKSEFFPKEIYVFTPKGRIVELPMGATAVDFAYAVHSDVGN
HHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCHHHHHHHHHHHCCCC
TCVGVTVEHKPYPLSKALESGQTVNIITDPNAHPEVAWLNFVVTARAKTRIRHYLKQRCE
CEEEEEEECCCCCHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EDAVKLGEVELNVALQPHNLGDFSIQQIRTVLDALALSSLDELLREIGLGNQSASMIAHQ
HHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FVGVPLESANTKNLEFESKILTIAPMQVGKTQFAQCCHPILGDPIVGCCTEKNTVVVHHQ
HHCCCCCCCCCCCCEECCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCEEEEEHH
HCASLKNACRQSLAKWDNVQSAVNFEAELQIEILNEQNALLSLMTAISASESSLQNIWTE
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHH
ELENNLLLVILQVCVKDIKHLANIVHRIKGITGVVNVKRNINEL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCHHHCCC
>Mature Secondary Structure
MELFADLDRIIQGYLPADKIELIKRAFVIARDAHEGQFRSSGEPYITHPVAVASIIAQLH
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECHHHHHHHHHHHH
LDHEAVMAALLHDVIEDTPYTEEQLKEEFGASVAEIVDGVSKLDKLKFRTRQEAQVENFR
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMILAMTRDIRVVLIKLADRTHNMRTLGSLRPDKRRRIAKETLEIYCPLAHRLGIEHIKN
HHHHHHHCCHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHCCHHHHCCHHHHHH
ELEDLSFQAMHPHRYEVLKKLVDVARSNRQDLIERISQEIKVRLENSGIFARVWGREKHL
HHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEECCCCEEEEECCCHHHH
YKIYQKMRIKDQEFHSIMDIYAFRVIVKNVDDCYRVLGQMHNLYKPRPGRVKDYIAVPKA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCC
NGYQSLQTSMIGPKGVPVEVHIHTEDMEQVAEMGITAHWVYKENGKNDSTTAQIRVQRWL
CCHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHH
QSLVEIQQSVGNSFEFIENVKSEFFPKEIYVFTPKGRIVELPMGATAVDFAYAVHSDVGN
HHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCHHHHHHHHHHHCCCC
TCVGVTVEHKPYPLSKALESGQTVNIITDPNAHPEVAWLNFVVTARAKTRIRHYLKQRCE
CEEEEEEECCCCCHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EDAVKLGEVELNVALQPHNLGDFSIQQIRTVLDALALSSLDELLREIGLGNQSASMIAHQ
HHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
FVGVPLESANTKNLEFESKILTIAPMQVGKTQFAQCCHPILGDPIVGCCTEKNTVVVHHQ
HHCCCCCCCCCCCCEECCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCEEEEEHH
HCASLKNACRQSLAKWDNVQSAVNFEAELQIEILNEQNALLSLMTAISASESSLQNIWTE
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHH
ELENNLLLVILQVCVKDIKHLANIVHRIKGITGVVNVKRNINEL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]