Click here to switch to the map view.

The map label for this gene is pdxT

Identifier: 30995464

GI number: 30995464

Start: 1713466

End: 1714044

Strand: Direct

Name: pdxT

Synonym: HI1648

Alternate gene names: 30995464

Gene position: 1713466-1714044 (Clockwise)

Preceding gene: 16273535

Following gene: 16273538

Centisome position: 93.62

GC content: 36.44

Gene sequence:

>579_bases
ATGAAAATCGGAATATTAGCTCTACAAGGTGCCTTTGCGGAACATGCACGAATGCTAGAAAAATTAGGAATTGAAAGTGT
CGAACTGAGAAATTTAAAAAATTTTCAACAACATTACAGTGATTTATCAGGTTTGATTCTACCTGGCGGTGAGTCAACCG
CCATAGGAAAACTTTTAAGAGAGCTGTATATGCTGGAACCGATAAAACAAGCTATCTCTTCTGGCTTTCCTGTCTTTGGA
ACTTGTGCTGGTTTGATTCTGTTGGCTAAAGAGATTACTTCTCAGAAAGAGAGTCATTTTGGAACAATGGACATTGTGGT
TGAGAGGAATGCCTATGGACGCCAATTGGGAAGTTTCTATACAGAAGCAGATTGCAAAGGGGTTGGTAAAATTCCTATGA
CTTTTATCAGAGGACCTATCATCAGTAGTGTTGGTAAAAAAGTCAATATTCTTGCAACGGTAAATAATAAAATCGTTGCA
GCCCAAGAAAAGAATATGCTGGTAACATCATTTCATCCTGAATTAACAAATAACTTGAGTTTGCATAAATACTTTATCGA
TATATGTAAAGTAGCATAA

Upstream 100 bases:

>100_bases
TCCACAAATCCTAGCACAAATCTCCGAAGATTTAGGTGAAGCCATGGTTGGTATCAATGAAAATGAAATCCAAATTCTCA
TGGCTGAGCGAGGAAAATAA

Downstream 100 bases:

>100_bases
ACCATTATCTGCATATCTGCCCTCCCTAGGAAACAAACCATATTGTAGCAATGAGAGCAGATAATTTTCATCAAAAAACC
GAACAGTTACTAGACTTAAA

Product: glutamine amidotransferase subunit PdxT

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MKIGILALQGAFAEHARMLEKLGIESVELRNLKNFQQHYSDLSGLILPGGESTAIGKLLRELYMLEPIKQAISSGFPVFG
TCAGLILLAKEITSQKESHFGTMDIVVERNAYGRQLGSFYTEADCKGVGKIPMTFIRGPIISSVGKKVNILATVNNKIVA
AQEKNMLVTSFHPELTNNLSLHKYFIDICKVA

Sequences:

>Translated_192_residues
MKIGILALQGAFAEHARMLEKLGIESVELRNLKNFQQHYSDLSGLILPGGESTAIGKLLRELYMLEPIKQAISSGFPVFG
TCAGLILLAKEITSQKESHFGTMDIVVERNAYGRQLGSFYTEADCKGVGKIPMTFIRGPIISSVGKKVNILATVNNKIVA
AQEKNMLVTSFHPELTNNLSLHKYFIDICKVA
>Mature_192_residues
MKIGILALQGAFAEHARMLEKLGIESVELRNLKNFQQHYSDLSGLILPGGESTAIGKLLRELYMLEPIKQAISSGFPVFG
TCAGLILLAKEITSQKESHFGTMDIVVERNAYGRQLGSFYTEADCKGVGKIPMTFIRGPIISSVGKKVNILATVNNKIVA
AQEKNMLVTSFHPELTNNLSLHKYFIDICKVA

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=204, Percent_Identity=33.8235294117647, Blast_Score=107, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6321048, Length=208, Percent_Identity=34.1346153846154, Blast_Score=104, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6323995, Length=208, Percent_Identity=33.6538461538462, Blast_Score=104, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_HAEI8 (Q4QJU4)

Other databases:

- EMBL:   CP000057
- RefSeq:   YP_249363.1
- HSSP:   Q5ND68
- ProteinModelPortal:   Q4QJU4
- SMR:   Q4QJU4
- STRING:   Q4QJU4
- GeneID:   3431203
- GenomeReviews:   CP000057_GR
- KEGG:   hit:NTHI1951
- eggNOG:   COG0311
- HOGENOM:   HBG292341
- OMA:   QGDVREH
- PhylomeDB:   Q4QJU4
- ProtClustDB:   PRK13525
- BioCyc:   HINF281310:NTHI1951-MONOMER
- HAMAP:   MF_01615
- InterPro:   IPR002161
- InterPro:   IPR021196
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: NA

Molecular weight: Translated: 21070; Mature: 21070

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 82-82 ACT_SITE 172-172 ACT_SITE 174-174 BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGILALQGAFAEHARMLEKLGIESVELRNLKNFQQHYSDLSGLILPGGESTAIGKLLR
CCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHH
ELYMLEPIKQAISSGFPVFGTCAGLILLAKEITSQKESHFGTMDIVVERNAYGRQLGSFY
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHH
TEADCKGVGKIPMTFIRGPIISSVGKKVNILATVNNKIVAAQEKNMLVTSFHPELTNNLS
HCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCEEEEECCCCEEEEECCCHHHCCCH
LHKYFIDICKVA
HHHHHHHHHHCC
>Mature Secondary Structure
MKIGILALQGAFAEHARMLEKLGIESVELRNLKNFQQHYSDLSGLILPGGESTAIGKLLR
CCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHH
ELYMLEPIKQAISSGFPVFGTCAGLILLAKEITSQKESHFGTMDIVVERNAYGRQLGSFY
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHH
TEADCKGVGKIPMTFIRGPIISSVGKKVNILATVNNKIVAAQEKNMLVTSFHPELTNNLS
HCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCEEEEECCCCEEEEECCCHHHCCCH
LHKYFIDICKVA
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA