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The map label for this gene is nagK [H]

Identifier: 30995357

GI number: 30995357

Start: 195915

End: 196829

Strand: Direct

Name: nagK [H]

Synonym: HI0182

Alternate gene names: 30995357

Gene position: 195915-196829 (Clockwise)

Preceding gene: 16272142

Following gene: 16272148

Centisome position: 10.7

GC content: 40.11

Gene sequence:

>915_bases
ATGTATTATGGTTTAGATATTGGTGGCACGAAAATAGAGCTTGCCGTATTCAATGAAAAATTGGAAAAATTATATTCTGA
ACGGGTGCCAACACCTAAAACCGACTATGAAGAATGGCTTAACACGATTGTGGATTTAGTGAATCGTGCTGATGAAAAGT
TTGGTGAGGTGGGCACCGTAGGTTTGGGCGTACCTGGTTTTGTTAATCAACAAACTGGATTAGCCGAAATTGCCAATATT
CGTGTGGCAGATAATAAACCAATTTTATGCGATTTGTCTACGCGTTTGGGCCGTGAAGTGCGGGCAGAAAATGACGCAAA
TTGTTTTGCGTTATCTGAAGCATGGGATACGGAAAATCAGCAATATTCGACTGTACTTGGTTTAATTCTTGGGACTGGTT
TTGGTGGTGGCTTTGTCTTAAACGGTAAAGTTCATTCAGGTCAAGTGGGAATGGCGGGAGAGCTTGGTCATTTACAATTA
AATTATCATGCTTTGAAATTATTGGGATGGGATAACGCCCCAATTTATCAATGCGGTTGCGGCAACAAGGCTTGTTTGGA
TAATTATCTGTCTGGTCGCGGTTTTGAAATGCTTTATCAAGATTTGAAAGGCGAAACATTATCTGCTCGTAAAATTATTA
ATTTGTTCTATCAAAGCAATGAAAGTGCGGTAGATTTTGTGAATCTTTTTGTTGAGTTGGCGGCGATTTCTATTGGCAAT
ATCATTACGGCATTTGATCCGCATATGATTGTGTTAGGTGGTGGATTATCTAATTTTGATTACCTTTATGAGGCTTTACC
AAAAGCGCTTCCGCCTCATTTGATGCGAAAAGCAAAAGTTCCGCCAATTAAAAAAGCGAAACACGGCGATTCTGGTGGTG
TGCGTGGTGCGGCAGCGTTGTTTTTAACCAAATAA

Upstream 100 bases:

>100_bases
TCTTTTTGAAGAAAAAGGCGATCTTTGTCATAAAATAATGATTTAAGTGCGGTTAAAATTTGGTTAATTTTAAGTGCACT
TCTTTATACAGGAGCAGATT

Downstream 100 bases:

>100_bases
TTCAACAAACTAGTGGGGCGTATTCAAATAATACGCCCTTTATTTTGGAAAATATTTAGAATATTGTGAATTTATTAGAT
TTTTTCTTGTTTGTGTTTCT

Product: N-acetyl-D-glucosamine kinase

Products: NA

Alternate protein names: GlcNAc kinase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MYYGLDIGGTKIELAVFNEKLEKLYSERVPTPKTDYEEWLNTIVDLVNRADEKFGEVGTVGLGVPGFVNQQTGLAEIANI
RVADNKPILCDLSTRLGREVRAENDANCFALSEAWDTENQQYSTVLGLILGTGFGGGFVLNGKVHSGQVGMAGELGHLQL
NYHALKLLGWDNAPIYQCGCGNKACLDNYLSGRGFEMLYQDLKGETLSARKIINLFYQSNESAVDFVNLFVELAAISIGN
IITAFDPHMIVLGGGLSNFDYLYEALPKALPPHLMRKAKVPPIKKAKHGDSGGVRGAAALFLTK

Sequences:

>Translated_304_residues
MYYGLDIGGTKIELAVFNEKLEKLYSERVPTPKTDYEEWLNTIVDLVNRADEKFGEVGTVGLGVPGFVNQQTGLAEIANI
RVADNKPILCDLSTRLGREVRAENDANCFALSEAWDTENQQYSTVLGLILGTGFGGGFVLNGKVHSGQVGMAGELGHLQL
NYHALKLLGWDNAPIYQCGCGNKACLDNYLSGRGFEMLYQDLKGETLSARKIINLFYQSNESAVDFVNLFVELAAISIGN
IITAFDPHMIVLGGGLSNFDYLYEALPKALPPHLMRKAKVPPIKKAKHGDSGGVRGAAALFLTK
>Mature_304_residues
MYYGLDIGGTKIELAVFNEKLEKLYSERVPTPKTDYEEWLNTIVDLVNRADEKFGEVGTVGLGVPGFVNQQTGLAEIANI
RVADNKPILCDLSTRLGREVRAENDANCFALSEAWDTENQQYSTVLGLILGTGFGGGFVLNGKVHSGQVGMAGELGHLQL
NYHALKLLGWDNAPIYQCGCGNKACLDNYLSGRGFEMLYQDLKGETLSARKIINLFYQSNESAVDFVNLFVELAAISIGN
IITAFDPHMIVLGGGLSNFDYLYEALPKALPPHLMRKAKVPPIKKAKHGDSGGVRGAAALFLTK

Specific function: Catalyzes the phosphorylation of N-acetyl-D-glucosamine (GlcNAc) derived from cell-wall degradation, yielding GlcNAc-6-P [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family. NagK subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787363, Length=303, Percent_Identity=49.8349834983498, Blast_Score=309, Evalue=2e-85,
Organism=Escherichia coli, GI87081733, Length=302, Percent_Identity=40.7284768211921, Blast_Score=227, Evalue=7e-61,
Organism=Escherichia coli, GI1790522, Length=314, Percent_Identity=25.796178343949, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.59 [H]

Molecular weight: Translated: 33107; Mature: 33107

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS01125 ROK ; PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYYGLDIGGTKIELAVFNEKLEKLYSERVPTPKTDYEEWLNTIVDLVNRADEKFGEVGTV
CEEEEECCCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
GLGVPGFVNQQTGLAEIANIRVADNKPILCDLSTRLGREVRAENDANCFALSEAWDTENQ
CCCCCCCCCCCCCHHHHHEEEEECCCCEEEECHHHHCHHHCCCCCCCEEEEECCCCCCCH
QYSTVLGLILGTGFGGGFVLNGKVHSGQVGMAGELGHLQLNYHALKLLGWDNAPIYQCGC
HHHHHHHHHHHCCCCCCEEEECEEECCCCCCCCCCCEEEEEEEEEEEEECCCCCEEEECC
GNKACLDNYLSGRGFEMLYQDLKGETLSARKIINLFYQSNESAVDFVNLFVELAAISIGN
CCHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
IITAFDPHMIVLGGGLSNFDYLYEALPKALPPHLMRKAKVPPIKKAKHGDSGGVRGAAAL
EEEECCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCEEEE
FLTK
EEEC
>Mature Secondary Structure
MYYGLDIGGTKIELAVFNEKLEKLYSERVPTPKTDYEEWLNTIVDLVNRADEKFGEVGTV
CEEEEECCCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
GLGVPGFVNQQTGLAEIANIRVADNKPILCDLSTRLGREVRAENDANCFALSEAWDTENQ
CCCCCCCCCCCCCHHHHHEEEEECCCCEEEECHHHHCHHHCCCCCCCEEEEECCCCCCCH
QYSTVLGLILGTGFGGGFVLNGKVHSGQVGMAGELGHLQLNYHALKLLGWDNAPIYQCGC
HHHHHHHHHHHCCCCCCEEEECEEECCCCCCCCCCCEEEEEEEEEEEEECCCCCEEEECC
GNKACLDNYLSGRGFEMLYQDLKGETLSARKIINLFYQSNESAVDFVNLFVELAAISIGN
CCHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
IITAFDPHMIVLGGGLSNFDYLYEALPKALPPHLMRKAKVPPIKKAKHGDSGGVRGAAAL
EEEECCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCEEEE
FLTK
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA