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The map label for this gene is nusB [H]

Identifier: 30410822

GI number: 30410822

Start: 509330

End: 509758

Strand: Direct

Name: nusB [H]

Synonym: BUsg447

Alternate gene names: 30410822

Gene position: 509330-509758 (Clockwise)

Preceding gene: 21672711

Following gene: 21672716

Centisome position: 79.4

GC content: 24.71

Gene sequence:

>429_bases
TTGAGAAAACAAATGATAATAAAACCAAATTTTCGAAGAAAAGCTCGCGCCTGTGCTTTACAAATGTTATATTCTTGGGA
AATTTCTCAAAATAATATAAAAGATAGTGCAATAGAATTTCTAAAAGAAAAAAATAAAAAAAACATTGATATTATATATT
TCTATGAATTAATTATAGGTATAACCTATAATTGTAGAAGTATAGATGATTTGATGAAACCATATTTATCTCGTTCACTA
AAAGAACTAGGACAAATTGAAAAAGCAATTCTCAGAATTTCGTTTTATGAGCTTTATAAAAGAAAGGATATACCTTATAA
GGTATCTATCAACGAAGGTATTGAATTAGCAAAATTATTTGGATCTGAAGATAGTCATAAGTTTATTAATGGTGTTTTAG
ATAAAGCAGCATTAAAAATTAAAAGATAA

Upstream 100 bases:

>100_bases
TGATTGTCTTAAATTTGATTTTAAAGACATTCGTCAAATAGGACCTGATATACGAATAAAATTAGAGCCTAAAAATAAAA
AATTTTAAAATAAAGTATAA

Downstream 100 bases:

>100_bases
ATAATAATTAAAAAATTTTTATTATTCTAATATGACAACCAAGCAAGTATTTTGTTTTGAATGCCTTCCGGATCTAATTG
ATAATCATGTTTAATCTCTT

Product: transcription antitermination protein NusB

Products: NA

Alternate protein names: Protein nusB [H]

Number of amino acids: Translated: 142; Mature: 142

Protein sequence:

>142_residues
MRKQMIIKPNFRRKARACALQMLYSWEISQNNIKDSAIEFLKEKNKKNIDIIYFYELIIGITYNCRSIDDLMKPYLSRSL
KELGQIEKAILRISFYELYKRKDIPYKVSINEGIELAKLFGSEDSHKFINGVLDKAALKIKR

Sequences:

>Translated_142_residues
MRKQMIIKPNFRRKARACALQMLYSWEISQNNIKDSAIEFLKEKNKKNIDIIYFYELIIGITYNCRSIDDLMKPYLSRSL
KELGQIEKAILRISFYELYKRKDIPYKVSINEGIELAKLFGSEDSHKFINGVLDKAALKIKR
>Mature_142_residues
MRKQMIIKPNFRRKARACALQMLYSWEISQNNIKDSAIEFLKEKNKKNIDIIYFYELIIGITYNCRSIDDLMKPYLSRSL
KELGQIEKAILRISFYELYKRKDIPYKVSINEGIELAKLFGSEDSHKFINGVLDKAALKIKR

Specific function: Involved in the transcription termination process [H]

COG id: COG0781

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nusB family [H]

Homologues:

Organism=Escherichia coli, GI1786618, Length=135, Percent_Identity=62.2222222222222, Blast_Score=176, Evalue=6e-46,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011605
- InterPro:   IPR006027 [H]

Pfam domain/function: PF01029 NusB [H]

EC number: NA

Molecular weight: Translated: 16787; Mature: 16787

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKQMIIKPNFRRKARACALQMLYSWEISQNNIKDSAIEFLKEKNKKNIDIIYFYELIIG
CCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHH
ITYNCRSIDDLMKPYLSRSLKELGQIEKAILRISFYELYKRKDIPYKVSINEGIELAKLF
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHH
GSEDSHKFINGVLDKAALKIKR
CCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRKQMIIKPNFRRKARACALQMLYSWEISQNNIKDSAIEFLKEKNKKNIDIIYFYELIIG
CCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHH
ITYNCRSIDDLMKPYLSRSLKELGQIEKAILRISFYELYKRKDIPYKVSINEGIELAKLF
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHH
GSEDSHKFINGVLDKAALKIKR
CCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]