| Definition | Bacillus anthracis str. Ames, complete genome. |
|---|---|
| Accession | NC_003997 |
| Length | 5,227,293 |
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The map label for this gene is priA [H]
Identifier: 30263871
GI number: 30263871
Start: 3684017
End: 3686422
Strand: Reverse
Name: priA [H]
Synonym: BA_4006
Alternate gene names: 30263871
Gene position: 3686422-3684017 (Counterclockwise)
Preceding gene: 30263872
Following gene: 30263870
Centisome position: 70.52
GC content: 37.41
Gene sequence:
>2406_bases ATGAAATTTGCAAGTGTAATTGTTGATGTACCTGCACGTCAGACAGATCGACCATTTGATTATATTATCCCTAAAAAATG GGAAGATATTGTCCAGACAGGCATGCGTGTAGTAGTTCCGTTTGGCCCAAGGAAATTGCAAGGTTTTATTATTGGAATTA AAAATTCGGTTGAAGTAGAAAGTAAAAAGTTAAAGACAATCCACGAAATTTTAGATGTAACACCGGTTTTAAATGAAGAG TTGTTAAAGCTTGGGTATTGGCTTACAAGTGAAACGTTATGTTATATGATTTCAGCTTTTCAAGTTATGCTTCCGACGGC GATAAAAGCAACATATAAAAAACGTCTACAGCTTCGTAAACAAGAAGAAGTAGCACCTGAACTACTGTTTTTATTTCAGG AAAAAGAAGCGATAGATTGGGAAGCGATTGAGACGCAACCACATCTATACCGCACGATTCAACAAGAAATTAAACATGGC ACGATTGAAGTCGTTTATCAAGTAAAAGATAAAGTGCAAAAGAAGAAACAAAGAGTCGTTCAACCGGAGTTGCCAGAAGA TAAATTAGAATTGGCAGCATTTGAACTGAAAAGTAAAAAACAACAAGATGTCCTCTATTATTTTGTGGAAAATTATAAAA GTGTGCCGTTGAAGGTAATAACAGAAGAGTTGCAAATAACAGATGCTCCGATTAAAGCACTTGTTAAAAAGGGGCTAATC TCAGAAAAGTATGTGGAAGTATATCGAAATCCGTATGACGATGAAGATTTTGAACAAACGAAACCATTCCCACTTACTGA GGAACAAAAGCAAGTTATTACACCGATTTTATCATCAATTACAAATGAAACTTACAATCCATTTTTACTATATGGTGTTA CAGGAAGCGGAAAAACAGAAGTATATTTACAATCTATAGCAGCGGTGCTCGAGAAAGGAAAAGAAGCTATTGTACTTGTT CCTGAAATTGCACTAACGCCTCAGATGGTAGATCGTTTTAAAGGTAGATTTGGCTCACAAGTTGCGGTTCTTCATAGTGC GTTGTCTGTTGGAGAAAAATATGATGAATGGCGTAAGATTTTAAGAAAAGAAGTGAAGGTTGTAGTTGGTGCACGTTCAG CTGTATTTGCTCCTTTTGAAAATTTAGGGATTATTATTATTGATGAGGAACATGAATCGAGTTATAAGCAAGAAGATAAT CCGAGGTATCATGCAAGGGATGTAGCTGTGTGGAGAGGACAATATCATAAATGTCCTATCGTTCTTGGTAGTGCAACACC GACGCTTGAATCATTTGCAAGAGCGAAAAAAGGTGTGTATGAATTACTGACGATGGAAAAACGTATGAACGAACAAGCTT TACCGACAGTAGAGATTGTTGATATGCGTGAGGAACTTCGTGACGGGAATCGCTCCATGTTTTCAAAGGCATTGCATGAA AAAATAGCAGATCGATTAGAAAAGAAAGAACAAATGGTGCTCTTTTTAAATAGAAGGGGGCATTCTACATTTGTTATGTG CCGTGATTGCGGGTACGTTGTACAATGTCCGCATTGTGATATCTCACTAACATATCATAAAATGAACCATCGTTTAAAAT GCCATTATTGCAGTTACGAAGAAAATATGCCGACTGCGTGTCCTGCTTGTCAAAGTACATACATTCGTTTCTTTGGTACG GGTACGCAAAAGGTAGAAGAAGAAATTACAAAACTATTTCCAGAGGCACGAGTCATTCGAATGGATGTAGATACGACGAG TCGTAAAGGGATGCATGAGAAATTATTAAAGGCGTTCGGGGAAGAAAAAGCAGATATATTACTTGGAACACAAATGATTG CGAAGGGGTTAGATTTTCCGAAAGTAACGCTTGTTGGGGTTTTAACTGCTGATACGATGCTTCATTTACCTGATTTTAGG GCGAGTGAAAAGACTTATCAGTTATTGACGCAAGTAAGTGGACGAGCAGGCCGACATGAATTGCCAGGGGAAGTTATAAT TCAAACGTATACACCAGAACATTACAGTATAGAGTTAGCAAAGAACCAACAATACGATGTGTTTTTTGACCAAGAAATGC AAATGAGACGAACGAGACAATATCCACCTTATTACTATGTTGTACTTGTGACGGTATCTCACCCGGAATTATTGAAGGCA GTACAAGTGACGGAAAAAATTGTCGGTCATTTACGATCGCACTGCTCGCAGCAAACAATGGTGTTAGGACCGGTTGCTTC AGCAATTCCAAGGATAAAAGATAGATATCGTTATCAATGCATGATAAAATACAAACGGGAACCAAACTTAAAGAACGTGC TCAAAATGGTAAATGAACATTATCAAGCAGAAATGCAAAAAGAGCTACAAATCTCAATTGATTTTAATCCAACAATGTTA ATGTAG
Upstream 100 bases:
>100_bases GAAAAGATGGAGAAGTTATAGAGTTGCCACTTTTAACGAAGAAAGAGGTTGCTCGTGAAATATTAAAGCAAATTGAAATG ATGTTAGAAGATGATCGTCT
Downstream 100 bases:
>100_bases CGGAGGGAAATATGGCAGTTTTAGAAATAGTAAAGCATCCAAATGAAGTGTTAGAAACACCTTGTGAAAGAGTAATTAAC TTTGATAAAAAGTTAGTGAA
Product: primosome assembly protein PriA
Products: NA
Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]
Number of amino acids: Translated: 801; Mature: 801
Protein sequence:
>801_residues MKFASVIVDVPARQTDRPFDYIIPKKWEDIVQTGMRVVVPFGPRKLQGFIIGIKNSVEVESKKLKTIHEILDVTPVLNEE LLKLGYWLTSETLCYMISAFQVMLPTAIKATYKKRLQLRKQEEVAPELLFLFQEKEAIDWEAIETQPHLYRTIQQEIKHG TIEVVYQVKDKVQKKKQRVVQPELPEDKLELAAFELKSKKQQDVLYYFVENYKSVPLKVITEELQITDAPIKALVKKGLI SEKYVEVYRNPYDDEDFEQTKPFPLTEEQKQVITPILSSITNETYNPFLLYGVTGSGKTEVYLQSIAAVLEKGKEAIVLV PEIALTPQMVDRFKGRFGSQVAVLHSALSVGEKYDEWRKILRKEVKVVVGARSAVFAPFENLGIIIIDEEHESSYKQEDN PRYHARDVAVWRGQYHKCPIVLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVEIVDMREELRDGNRSMFSKALHE KIADRLEKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNHRLKCHYCSYEENMPTACPACQSTYIRFFGT GTQKVEEEITKLFPEARVIRMDVDTTSRKGMHEKLLKAFGEEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFR ASEKTYQLLTQVSGRAGRHELPGEVIIQTYTPEHYSIELAKNQQYDVFFDQEMQMRRTRQYPPYYYVVLVTVSHPELLKA VQVTEKIVGHLRSHCSQQTMVLGPVASAIPRIKDRYRYQCMIKYKREPNLKNVLKMVNEHYQAEMQKELQISIDFNPTML M
Sequences:
>Translated_801_residues MKFASVIVDVPARQTDRPFDYIIPKKWEDIVQTGMRVVVPFGPRKLQGFIIGIKNSVEVESKKLKTIHEILDVTPVLNEE LLKLGYWLTSETLCYMISAFQVMLPTAIKATYKKRLQLRKQEEVAPELLFLFQEKEAIDWEAIETQPHLYRTIQQEIKHG TIEVVYQVKDKVQKKKQRVVQPELPEDKLELAAFELKSKKQQDVLYYFVENYKSVPLKVITEELQITDAPIKALVKKGLI SEKYVEVYRNPYDDEDFEQTKPFPLTEEQKQVITPILSSITNETYNPFLLYGVTGSGKTEVYLQSIAAVLEKGKEAIVLV PEIALTPQMVDRFKGRFGSQVAVLHSALSVGEKYDEWRKILRKEVKVVVGARSAVFAPFENLGIIIIDEEHESSYKQEDN PRYHARDVAVWRGQYHKCPIVLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVEIVDMREELRDGNRSMFSKALHE KIADRLEKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNHRLKCHYCSYEENMPTACPACQSTYIRFFGT GTQKVEEEITKLFPEARVIRMDVDTTSRKGMHEKLLKAFGEEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFR ASEKTYQLLTQVSGRAGRHELPGEVIIQTYTPEHYSIELAKNQQYDVFFDQEMQMRRTRQYPPYYYVVLVTVSHPELLKA VQVTEKIVGHLRSHCSQQTMVLGPVASAIPRIKDRYRYQCMIKYKREPNLKNVLKMVNEHYQAEMQKELQISIDFNPTML M >Mature_801_residues MKFASVIVDVPARQTDRPFDYIIPKKWEDIVQTGMRVVVPFGPRKLQGFIIGIKNSVEVESKKLKTIHEILDVTPVLNEE LLKLGYWLTSETLCYMISAFQVMLPTAIKATYKKRLQLRKQEEVAPELLFLFQEKEAIDWEAIETQPHLYRTIQQEIKHG TIEVVYQVKDKVQKKKQRVVQPELPEDKLELAAFELKSKKQQDVLYYFVENYKSVPLKVITEELQITDAPIKALVKKGLI SEKYVEVYRNPYDDEDFEQTKPFPLTEEQKQVITPILSSITNETYNPFLLYGVTGSGKTEVYLQSIAAVLEKGKEAIVLV PEIALTPQMVDRFKGRFGSQVAVLHSALSVGEKYDEWRKILRKEVKVVVGARSAVFAPFENLGIIIIDEEHESSYKQEDN PRYHARDVAVWRGQYHKCPIVLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVEIVDMREELRDGNRSMFSKALHE KIADRLEKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNHRLKCHYCSYEENMPTACPACQSTYIRFFGT GTQKVEEEITKLFPEARVIRMDVDTTSRKGMHEKLLKAFGEEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFR ASEKTYQLLTQVSGRAGRHELPGEVIIQTYTPEHYSIELAKNQQYDVFFDQEMQMRRTRQYPPYYYVVLVTVSHPELLKA VQVTEKIVGHLRSHCSQQTMVLGPVASAIPRIKDRYRYQCMIKYKREPNLKNVLKMVNEHYQAEMQKELQISIDFNPTML M
Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase
COG id: COG1198
COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 helicase C-terminal domain [H]
Homologues:
Organism=Escherichia coli, GI1790370, Length=556, Percent_Identity=39.568345323741, Blast_Score=421, Evalue=1e-118, Organism=Escherichia coli, GI1787357, Length=191, Percent_Identity=25.130890052356, Blast_Score=65, Evalue=2e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR014001 - InterPro: IPR011545 - InterPro: IPR001650 - InterPro: IPR014021 - InterPro: IPR005259 [H]
Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]
EC number: NA
Molecular weight: Translated: 92640; Mature: 92640
Theoretical pI: Translated: 8.25; Mature: 8.25
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.5 %Cys (Translated Protein) 3.1 %Met (Translated Protein) 4.6 %Cys+Met (Translated Protein) 1.5 %Cys (Mature Protein) 3.1 %Met (Mature Protein) 4.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKFASVIVDVPARQTDRPFDYIIPKKWEDIVQTGMRVVVPFGPRKLQGFIIGIKNSVEVE CCHHHEEEECCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCEEEEEEEEECCCCCHH SKKLKTIHEILDVTPVLNEELLKLGYWLTSETLCYMISAFQVMLPTAIKATYKKRLQLRK HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH QEEVAPELLFLFQEKEAIDWEAIETQPHLYRTIQQEIKHGTIEVVYQVKDKVQKKKQRVV HHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHCC QPELPEDKLELAAFELKSKKQQDVLYYFVENYKSVPLKVITEELQITDAPIKALVKKGLI CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHCCC SEKYVEVYRNPYDDEDFEQTKPFPLTEEQKQVITPILSSITNETYNPFLLYGVTGSGKTE HHHHHHHHCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHH VYLQSIAAVLEKGKEAIVLVPEIALTPQMVDRFKGRFGSQVAVLHSALSVGEKYDEWRKI HHHHHHHHHHHCCCCEEEEECCHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH LRKEVKVVVGARSAVFAPFENLGIIIIDEEHESSYKQEDNPRYHARDVAVWRGQYHKCPI HHHHHHHHCCCCHHEECCHHHCCEEEEECCCCCCCCCCCCCCEEHHHHHEECCCCCCCCE VLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVEIVDMREELRDGNRSMFSKALHE EECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHCCCCHHHHHHHHHH KIADRLEKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNHRLKCHYCSYE HHHHHHHHHCCEEEEEECCCCCEEEEEECCCCEEECCCCCEEEEEECCCCCEEEEEECCC ENMPTACPACQSTYIRFFGTGTQKVEEEITKLFPEARVIRMDVDTTSRKGMHEKLLKAFG CCCCCCCCHHHHCEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHC EEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFRASEKTYQLLTQVSGRAGRHE CCCHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHEECCCCCCCHHHHHHHHHHCCCCCCCC LPGEVIIQTYTPEHYSIELAKNQQYDVFFDQEMQMRRTRQYPPYYYVVLVTVSHPELLKA CCCCEEEEEECCCCEEEEEECCCCEEEEECHHHHHHHHCCCCCEEEEEEEEECCHHHHHH VQVTEKIVGHLRSHCSQQTMVLGPVASAIPRIKDRYRYQCMIKYKREPNLKNVLKMVNEH HHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHH YQAEMQKELQISIDFNPTMLM HHHHHCCCEEEEEECCCCCCC >Mature Secondary Structure MKFASVIVDVPARQTDRPFDYIIPKKWEDIVQTGMRVVVPFGPRKLQGFIIGIKNSVEVE CCHHHEEEECCCCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCEEEEEEEEECCCCCHH SKKLKTIHEILDVTPVLNEELLKLGYWLTSETLCYMISAFQVMLPTAIKATYKKRLQLRK HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH QEEVAPELLFLFQEKEAIDWEAIETQPHLYRTIQQEIKHGTIEVVYQVKDKVQKKKQRVV HHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHCC QPELPEDKLELAAFELKSKKQQDVLYYFVENYKSVPLKVITEELQITDAPIKALVKKGLI CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHCCC SEKYVEVYRNPYDDEDFEQTKPFPLTEEQKQVITPILSSITNETYNPFLLYGVTGSGKTE HHHHHHHHCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHH VYLQSIAAVLEKGKEAIVLVPEIALTPQMVDRFKGRFGSQVAVLHSALSVGEKYDEWRKI HHHHHHHHHHHCCCCEEEEECCHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH LRKEVKVVVGARSAVFAPFENLGIIIIDEEHESSYKQEDNPRYHARDVAVWRGQYHKCPI HHHHHHHHCCCCHHEECCHHHCCEEEEECCCCCCCCCCCCCCEEHHHHHEECCCCCCCCE VLGSATPTLESFARAKKGVYELLTMEKRMNEQALPTVEIVDMREELRDGNRSMFSKALHE EECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHCCCCHHHHHHHHHH KIADRLEKKEQMVLFLNRRGHSTFVMCRDCGYVVQCPHCDISLTYHKMNHRLKCHYCSYE HHHHHHHHHCCEEEEEECCCCCEEEEEECCCCEEECCCCCEEEEEECCCCCEEEEEECCC ENMPTACPACQSTYIRFFGTGTQKVEEEITKLFPEARVIRMDVDTTSRKGMHEKLLKAFG CCCCCCCCHHHHCEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHC EEKADILLGTQMIAKGLDFPKVTLVGVLTADTMLHLPDFRASEKTYQLLTQVSGRAGRHE CCCHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHEECCCCCCCHHHHHHHHHHCCCCCCCC LPGEVIIQTYTPEHYSIELAKNQQYDVFFDQEMQMRRTRQYPPYYYVVLVTVSHPELLKA CCCCEEEEEECCCCEEEEEECCCCEEEEECHHHHHHHHCCCCCEEEEEEEEECCHHHHHH VQVTEKIVGHLRSHCSQQTMVLGPVASAIPRIKDRYRYQCMIKYKREPNLKNVLKMVNEH HHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHH YQAEMQKELQISIDFNPTMLM HHHHHCCCEEEEEECCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9534248; 9384377; 9086272 [H]