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The map label for this gene is scrFIAM [H]

Identifier: 30263689

GI number: 30263689

Start: 3496987

End: 3497766

Strand: Reverse

Name: scrFIAM [H]

Synonym: BA_3814

Alternate gene names: 30263689

Gene position: 3497766-3496987 (Counterclockwise)

Preceding gene: 30263690

Following gene: 30263688

Centisome position: 66.91

GC content: 42.82

Gene sequence:

>780_bases
ATGTACAGAAGTAAGGAATCTGGACTGAAAATGTTAGATTTGTGTTCGGGAATTGCAGGAATAAGTATGGCAGCGGATTG
GGCTGGAATTGATACAGCAGCCTTTTGTGAAATAGAGGAGTTCAATCAAAAAGTACTTAGAAAGAACTATCCTAACATTC
CTATTTTCCCGGATTTATATAAACTTATGAAACAATCATTAATAGATGGAGGTGTTGACGTTGATTCAATTGGAGTTATT
TCAGCAGGATATCCCTGTCAGGGAGAAAGTCTTGTTGGGAAGCGAAGAGGTGCAGAAGACGAAAGATGGTTATGGCCAGA
AGTCTTCCGACTTATTAAAGAACTCCGGCCCACTTGGTTTGTTGGAGAAAATGTTGCTGGACACGTCACGATGGGCTTGG
ACACCGTGCTCTCCGACTTGGAAGAAGAAAACTACTCGACAAGGACGTTTGTATTTCCGGCTGTCAGTGTCGGCGCGCCG
CATCAAAGATACCGGACATTTATTGTTGGCCACTCCAACGACAAGTCAAAATTACAAACCGATCCGAGAGTTGTGCCCTT
CAGAAGCAAATGGGAAACATGGGAAAACACTACCGGGATCAATCGGGGAACACTTTCCGGAACATATTGGGAAGAAAATA
AACCCGCAATTTGTGGAATGGATGATGGGACTGCCACAAGATTGGACGAAGATAGATTGAGATTCTTAGGAAACGCGGTT
GTACCTCAGCAGATTTATCCGATATTTGAAGCGATAGCAAAGATTGAAGGTTTATTATAA

Upstream 100 bases:

>100_bases
TGTATGGATTTACCTATGAGGATGTTATTGATTCTTATAAGGAGAAGAATGCCGAAAACTTCGAGAGACAAGAAAATGGA
TATTAGGATGAGTTGGGTTT

Downstream 100 bases:

>100_bases
AAATTTCATTGTGTCATAAATAAAAAGGCGGTTGTTTCCGCAACCACCTTTCAGTAAAACAAAAGCACTTTTTCAGAAAT
AAAATATGGATGATTCCTGA

Product: prophage LambdaBa01, C-5 cytosine-specific DNA methylase family protein

Products: NA

Alternate protein names: M.ScrFI-A; M.ScrFIA; Cytosine-specific methyltransferase ScrFIA [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MYRSKESGLKMLDLCSGIAGISMAADWAGIDTAAFCEIEEFNQKVLRKNYPNIPIFPDLYKLMKQSLIDGGVDVDSIGVI
SAGYPCQGESLVGKRRGAEDERWLWPEVFRLIKELRPTWFVGENVAGHVTMGLDTVLSDLEEENYSTRTFVFPAVSVGAP
HQRYRTFIVGHSNDKSKLQTDPRVVPFRSKWETWENTTGINRGTLSGTYWEENKPAICGMDDGTATRLDEDRLRFLGNAV
VPQQIYPIFEAIAKIEGLL

Sequences:

>Translated_259_residues
MYRSKESGLKMLDLCSGIAGISMAADWAGIDTAAFCEIEEFNQKVLRKNYPNIPIFPDLYKLMKQSLIDGGVDVDSIGVI
SAGYPCQGESLVGKRRGAEDERWLWPEVFRLIKELRPTWFVGENVAGHVTMGLDTVLSDLEEENYSTRTFVFPAVSVGAP
HQRYRTFIVGHSNDKSKLQTDPRVVPFRSKWETWENTTGINRGTLSGTYWEENKPAICGMDDGTATRLDEDRLRFLGNAV
VPQQIYPIFEAIAKIEGLL
>Mature_259_residues
MYRSKESGLKMLDLCSGIAGISMAADWAGIDTAAFCEIEEFNQKVLRKNYPNIPIFPDLYKLMKQSLIDGGVDVDSIGVI
SAGYPCQGESLVGKRRGAEDERWLWPEVFRLIKELRPTWFVGENVAGHVTMGLDTVLSDLEEENYSTRTFVFPAVSVGAP
HQRYRTFIVGHSNDKSKLQTDPRVVPFRSKWETWENTTGINRGTLSGTYWEENKPAICGMDDGTATRLDEDRLRFLGNAV
VPQQIYPIFEAIAKIEGLL

Specific function: This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the ScrFI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525
- InterPro:   IPR001387
- InterPro:   IPR010982 [H]

Pfam domain/function: PF00145 DNA_methylase; PF01381 HTH_3 [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 29103; Mature: 29103

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRSKESGLKMLDLCSGIAGISMAADWAGIDTAAFCEIEEFNQKVLRKNYPNIPIFPDLY
CCCCHHHCCHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
KLMKQSLIDGGVDVDSIGVISAGYPCQGESLVGKRRGAEDERWLWPEVFRLIKELRPTWF
HHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEE
VGENVAGHVTMGLDTVLSDLEEENYSTRTFVFPAVSVGAPHQRYRTFIVGHSNDKSKLQT
ECCCCCCEEEECHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHEEEEEEECCCCHHHHCC
DPRVVPFRSKWETWENTTGINRGTLSGTYWEENKPAICGMDDGTATRLDEDRLRFLGNAV
CCCEECCCHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHCCCC
VPQQIYPIFEAIAKIEGLL
CHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYRSKESGLKMLDLCSGIAGISMAADWAGIDTAAFCEIEEFNQKVLRKNYPNIPIFPDLY
CCCCHHHCCHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
KLMKQSLIDGGVDVDSIGVISAGYPCQGESLVGKRRGAEDERWLWPEVFRLIKELRPTWF
HHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEE
VGENVAGHVTMGLDTVLSDLEEENYSTRTFVFPAVSVGAPHQRYRTFIVGHSNDKSKLQT
ECCCCCCEEEECHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHEEEEEEECCCCHHHHCC
DPRVVPFRSKWETWENTTGINRGTLSGTYWEENKPAICGMDDGTATRLDEDRLRFLGNAV
CCCEECCCHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHCCCC
VPQQIYPIFEAIAKIEGLL
CHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8481004 [H]