Click here to switch to the map view.

The map label for this gene is ykuN [H]

Identifier: 30263489

GI number: 30263489

Start: 3300779

End: 3301222

Strand: Reverse

Name: ykuN [H]

Synonym: BA_3596

Alternate gene names: 30263489

Gene position: 3301222-3300779 (Counterclockwise)

Preceding gene: 30263490

Following gene: 30263488

Centisome position: 63.15

GC content: 36.94

Gene sequence:

>444_bases
GTGGCGAAAATTTTAATAGCTTATGCAAGTATGTCAGGAAATACAGAGAGTATTGCTGATTTAATTAAAGTTAGTTTAGA
TGCTTTTGATCATGAAGTAGTATTGCAAGAAATGGAAGGCATGGATGCTGAAGAATTATTAGCTTATGATGGAATCATTT
TAGGATCTTATACGTGGGGAGATGGTGAATTGCCATTTGAAGCGGAAGATTTCCATGAGGACTTAGAAAATATTGATTTA
TCAGGGAAGAAAGTAGCGGTATTTGGATCAGGTGATACGGCGTATGAACTGTTTTGTGAAGCGGTAACGATATTTGAAGA
AAGGCTTGTAGAACGCGGTGCAGAGCTTGTACAAGAAGGATTGAAAATCGAACTAGCTCCAGAAGATGAAGAGGATGTTG
AAAAATGCAGTAATTTTGCAATTGCATTTGCTGAGAAGTTCTAA

Upstream 100 bases:

>100_bases
TATGAAGTAAACAGAAATAATAAAAAATACAATTGAAATTGATTCTCATTATTAATATAATGAGAATGAGAATCATTTAT
TGTTAGAGGGGGATGCCAAA

Downstream 100 bases:

>100_bases
GAATGGGAGTGTCAACGTGGAGACAATGTTAAGTGCGACCGCTATTACAAAGTTAAGAGATGGGAAGCTTATGTTAGCTA
CTACATATAATGGTTTATTT

Product: flavodoxin

Products: oxidized flavodoxin; NH3; ADP; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 147; Mature: 146

Protein sequence:

>147_residues
MAKILIAYASMSGNTESIADLIKVSLDAFDHEVVLQEMEGMDAEELLAYDGIILGSYTWGDGELPFEAEDFHEDLENIDL
SGKKVAVFGSGDTAYELFCEAVTIFEERLVERGAELVQEGLKIELAPEDEEDVEKCSNFAIAFAEKF

Sequences:

>Translated_147_residues
MAKILIAYASMSGNTESIADLIKVSLDAFDHEVVLQEMEGMDAEELLAYDGIILGSYTWGDGELPFEAEDFHEDLENIDL
SGKKVAVFGSGDTAYELFCEAVTIFEERLVERGAELVQEGLKIELAPEDEEDVEKCSNFAIAFAEKF
>Mature_146_residues
AKILIAYASMSGNTESIADLIKVSLDAFDHEVVLQEMEGMDAEELLAYDGIILGSYTWGDGELPFEAEDFHEDLENIDLS
GKKVAVFGSGDTAYELFCEAVTIFEERLVERGAELVQEGLKIELAPEDEEDVEKCSNFAIAFAEKF

Specific function: Low-potential electron donor to a number of redox enzymes (Potential) [H]

COG id: COG0716

COG function: function code C; Flavodoxins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Escherichia coli, GI1786900, Length=121, Percent_Identity=38.8429752066116, Blast_Score=67, Evalue=3e-13,

Paralogues:

None

Copy number: 242 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010087
- InterPro:   IPR001094
- InterPro:   IPR008254 [H]

Pfam domain/function: PF00258 Flavodoxin_1 [H]

EC number: 1.19.6.1

Molecular weight: Translated: 16256; Mature: 16125

Theoretical pI: Translated: 3.70; Mature: 3.70

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKILIAYASMSGNTESIADLIKVSLDAFDHEVVLQEMEGMDAEELLAYDGIILGSYTWG
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCEEEECEECC
DGELPFEAEDFHEDLENIDLSGKKVAVFGSGDTAYELFCEAVTIFEERLVERGAELVQEG
CCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LKIELAPEDEEDVEKCSNFAIAFAEKF
CEEEECCCCHHHHHHHHHHHHEEEECC
>Mature Secondary Structure 
AKILIAYASMSGNTESIADLIKVSLDAFDHEVVLQEMEGMDAEELLAYDGIILGSYTWG
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCEEEECEECC
DGELPFEAEDFHEDLENIDLSGKKVAVFGSGDTAYELFCEAVTIFEERLVERGAELVQEG
CCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LKIELAPEDEEDVEKCSNFAIAFAEKF
CEEEECCCCHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced flavodoxin; H+; N2; ATP

Specific reaction: 6 reduced flavodoxin + 6 H+ + N2 + n ATP = 6 oxidized flavodoxin + 2 NH3 + n ADP + n phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]