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The map label for this gene is hpaF

Identifier: 30065592

GI number: 30065592

Start: 4545266

End: 4545646

Strand: Reverse

Name: hpaF

Synonym: S4651

Alternate gene names: 30065592

Gene position: 4545646-4545266 (Counterclockwise)

Preceding gene: 30065593

Following gene: 30065591

Centisome position: 98.83

GC content: 49.61

Gene sequence:

>381_bases
ATGCCGCACTTTATCGTGGAATGTACTGAAAATATTCGTGAAGAAGCCCGCTTACCTGAGCTGTTTGCCAGTGTTAACAC
TGCACTGGCGGCAACGGGGATTTTTCCGCTTGGGGGAATACGTAGCCGGGCGCACTGGATCGATACCTGGCAAATGGCTG
ATGGCAAGCACGATTACGCCTTTGTGCATATGACGCTAAAAATCGGCAGCGGACGCAGTCTGGAAAGCCGTCAGGAAGTG
GGCGAAATGCTGTTTGATCTGATTAAAACGCACTTCGCCTCACTGATGGAGAGCCGTTACCTAGCGCTTTCATTTGAGAT
TGCAGAGCTGCACCCGACGTTGAATTTCAAACAGAACAACGTACACGCGTTGTTTAAATAA

Upstream 100 bases:

>100_bases
TAAATACGACGGCAAGGTGGAGTTTATTACCGAGCTGTTCCCAAGCTCTGGCACCGGTCAGGTTAACGCTGTTTTCCCGC
TGCCCGCGTAAGGACGTTTT

Downstream 100 bases:

>100_bases
ATTTGCACCAGCTTCCAGGCCGGATGATAAGGCGCTGCCGCATCCGGCAATGCCACAGGATATCGCTATGTTCGATAAAC
ACACCCACACCCTGATCGCC

Product: 5-carboxymethyl-2-hydroxymuconate delta-isomerase

Products: NA

Alternate protein names: 5-carboxymethyl-2-hydroxymuconic acid isomerase; CHM isomerase; CHMI [H]

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRAHWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEV
GEMLFDLIKTHFASLMESRYLALSFEIAELHPTLNFKQNNVHALFK

Sequences:

>Translated_126_residues
MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRAHWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEV
GEMLFDLIKTHFASLMESRYLALSFEIAELHPTLNFKQNNVHALFK
>Mature_125_residues
PHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRAHWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVG
EMLFDLIKTHFASLMESRYLALSFEIAELHPTLNFKQNNVHALFK

Specific function: Transforms 5-carboxymethyl-2-hydroxy-muconic acid (CHM) into 5-oxo-pent-3-ene-1,2,5-tricarboxylic acid (OPET) [H]

COG id: COG3232

COG function: function code E; 5-carboxymethyl-2-hydroxymuconate isomerase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004220
- InterPro:   IPR014347 [H]

Pfam domain/function: PF02962 CHMI [H]

EC number: =5.3.3.10 [H]

Molecular weight: Translated: 14361; Mature: 14229

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRAHWIDTWQMADGKHDYA
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEEEEECCCCCCCEE
FVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLALSFEIAELHPTLNFKQNN
EEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEECCCCCCCCCCCC
VHALFK
EEEECC
>Mature Secondary Structure 
PHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRAHWIDTWQMADGKHDYA
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHEEEEECCCCCCCEE
FVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLALSFEIAELHPTLNFKQNN
EEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEECCCCCCCCCCCC
VHALFK
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2194841; 2261999; 8547259 [H]