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The map label for this gene is yhfR

Identifier: 30065341

GI number: 30065341

Start: 4298702

End: 4299499

Strand: Reverse

Name: yhfR

Synonym: S4369

Alternate gene names: 30065341

Gene position: 4299499-4298702 (Counterclockwise)

Preceding gene: 30065342

Following gene: 229089130

Centisome position: 93.48

GC content: 50.25

Gene sequence:

>798_bases
GTGCTGTTACTTGCCGGAAAACGACCCTATAATCCGAGTAATTCATTCTTTATTTCAGGGTCGATTATGTCAGCTACGGA
CCGCTACTCTCATCAACTCCTCTACGCTACCGTCCGCCAGCGGCTGCTGGATGATATCGCGCAGGGGGTTTACCAGGCCG
GGCAACAGATCCCTACCGAAAACGAGCTTTGTACACAATATAACGTCAGCCGCATTACCATTCGCAAAGCCATCAGCGAC
TTAGTGGCAGACGGCGTACTGATCCGCTGGCAGGGAAAAGGCACCTTTGTACAAAGCCAGAAAGTTGAAAACGCCCTGCT
TACTGTCAGTGGTTTTACCGATTTTGGCGTCTCACAAGGCAAGTCGACGAAAGAGAAAGTGATCGAACAGGAACGGGTCA
GCGCCGCGCCGTTTTGCGAAAAGCTGAACATCCCCGGAAACAGCGAAGTGTTCCATCTCTGCCGGGTGATGTATCTCGAT
AAAGAGCCGCTGTTTATTGATAGTTCATGGATCCCGCTGTCGCGTTATCCTGACTTTGATGAGATTTACGTCGAAGGAAG
CTCCACCTATCAGTTATTTCAGGAGCGTTTTGACACGCGAGTGGTCAGCGACAAAAAGACCATCGATATCTTTGCCGCCA
CCCGCCCGCAGGCAAAATGGCTGAAATGCGAACTGGGCGAACCGTTGTTTCGCATCAGCAAAATCGCCTTTGATCAGAAT
GACAAACCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACGATTGATAATAAAAGACATTAA

Upstream 100 bases:

>100_bases
ACCGCAGGCGATGGCGCAGGGAACGGCGTGCGCGGCGAAAACCATTCAGTACCACGGTGCCTGGTAGGTATAACGTTGGC
GTGAGCATCTTCACGCCAAC

Downstream 100 bases:

>100_bases
CCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCACTGAATTTTACATCCCGCACGTTCCCCTCA
CCCTAACCCTCTCCCCAAAG

Product: DNA-binding transcriptional regulator FrlR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MLLLAGKRPYNPSNSFFISGSIMSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQYNVSRITIRKAISD
LVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTKEKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLD
KEPLFIDSSWIPLSRYPDFDEIYVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
DKPVHVSELFCRANRITLTIDNKRH

Sequences:

>Translated_265_residues
MLLLAGKRPYNPSNSFFISGSIMSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQYNVSRITIRKAISD
LVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTKEKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLD
KEPLFIDSSWIPLSRYPDFDEIYVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
DKPVHVSELFCRANRITLTIDNKRH
>Mature_265_residues
MLLLAGKRPYNPSNSFFISGSIMSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQYNVSRITIRKAISD
LVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTKEKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLD
KEPLFIDSSWIPLSRYPDFDEIYVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
DKPVHVSELFCRANRITLTIDNKRH

Specific function: May regulate the transcription of the frlABCD operon [H]

COG id: COG2188

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082252, Length=243, Percent_Identity=99.5884773662551, Blast_Score=506, Evalue=1e-145,
Organism=Escherichia coli, GI1790118, Length=223, Percent_Identity=24.6636771300448, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1786950, Length=166, Percent_Identity=30.7228915662651, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1788418, Length=226, Percent_Identity=29.2035398230088, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000524
- InterPro:   IPR011663
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00392 GntR; PF07702 UTRA [H]

EC number: NA

Molecular weight: Translated: 30232; Mature: 30232

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLLAGKRPYNPSNSFFISGSIMSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTE
CEEECCCCCCCCCCCEEEECCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NELCTQYNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQG
CCHHHHCCCHHHHHHHHHHHHHHCCEEEEECCCCCEEEHHHHCCEEEEEECCCCCCCCCC
KSTKEKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFD
CCHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCC
EIYVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
CEEEECCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHHCCCC
DKPVHVSELFCRANRITLTIDNKRH
CCCEEHHHHHHCCCEEEEEECCCCC
>Mature Secondary Structure
MLLLAGKRPYNPSNSFFISGSIMSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTE
CEEECCCCCCCCCCCEEEECCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NELCTQYNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQG
CCHHHHCCCHHHHHHHHHHHHHHCCEEEEECCCCCEEEHHHHCCEEEEEECCCCCCCCCC
KSTKEKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFD
CCHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCC
EIYVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
CEEEECCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEECCCCHHHHHHHHHCCCC
DKPVHVSELFCRANRITLTIDNKRH
CCCEEHHHHHHCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]