| Definition | Shigella flexneri 2a str. 2457T, complete genome. |
|---|---|
| Accession | NC_004741 |
| Length | 4,599,354 |
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The map label for this gene is mrcA
Identifier: 229089130
GI number: 229089130
Start: 4278065
End: 4280617
Strand: Reverse
Name: mrcA
Synonym: S4348
Alternate gene names: 229089130
Gene position: 4280617-4278065 (Counterclockwise)
Preceding gene: 30065341
Following gene: 30065319
Centisome position: 93.07
GC content: 55.27
Gene sequence:
>2553_bases GTGAAGTTCGTAAAGTATTTTTTGATCCTTGCAGTCTGTTGCATTCTGCTGGGAGCAGGCTCGATTTATGGCCTATACCG CTACATCGAGCCACAACTGCCGGATGTGGTGACATTAAAAGATGTTCGCCTGCAAATTCCGATGCAGATTTACAGCGCCG ATGGCGAGCTGATTGCTCAATACGGTGAGAAACGTCGTATTCCGGTTACGTTGGATCAAATCCCACCGGAGATGGTGAAA GCCTTTATCGCGACGGAAGACAGCCGCTTCTACGAGCATCACGGCGTTGACCCGGTGGGGATCTTCCGTGCGGCAAGTGT GGCGCTGTTCTCCGGCCACGCGTCACAAGGGGCGAGTACCATTACCCAGCAGCTGGCGAGAAATTTCTTCCTCAGTCCAG AACGCACGATGATGCGTAAGATTAAGGAAGTCTTCCTCGCGATTCGCATTGAACAGTTGCTGACGAAAGACGAAATCCTC GAGCTTTATCTGAACAAGATTTACCTTGGTTACCGCGCCTATGGTGTCGGTGCTGCGGCACAAGTCTATTTCGGTAAAAC GGTCGACCAACTGACGCTGAACGAAATGGCGGTGATAGCCGGGTTGCCGAAAGCGCCTTCCACCTTTAATCCGCTCTACT CAATGGATCGTGCCGTCGCGCGGCGTAACGTCGTACTGTCGCGGATGCTGGATGAAGGGTATATCACCCAGCAACAGTTC GATCAGACACGCACTGAGGCGATTAACGCTAATTATCACGCGCCGGAGATTGCTTTCTCTGCGCCGTACCTGAGCGAAAT GGTGCGCCAGGAGATGTATAACCGTTATGGCGAAAGTGCCTATGAAGACGGTTATCGCATTTACACCACCATCACCCGCA AAGTGCAGCAGGCCGCGCAGCAGGCGGTACGTAATAACGTGCTGGACTACGACATGCGCCACGGCTATCGCGGCCCGGCA AATGTGCTGTGGAAAGTGGGCGAGTCGGCGTGGGATAACAACAAGATTACCGATACGCTGAAGGCGCTGCCGACCTATGG TCCGCTGCTGCCTGCCGCAGTCACCAGCGCCAATCCTCAGGGAGCGACGGCGATGCTGGCGGACGGGTCGACCGTCGCAT TGAGTATGGAAGGCGTTCGCTGGGCGCGTCCTTACCGTTCGGATACTCAGCAGGGACCGACGCCGCGTAAAGTGACCGAT GTTCTGCAAACGGGTCAGCAAATCTGGGTTCGTCAGGTTGGCGATGCATGGTGGCTGGCACAAGTGCCGGAAGTGAACTC GGCGCTGGTGTCGATCAATCCGCAAAATGGTGCCGTTATGGCGCTGGTCGGTGGCTTTGATTTCAATCAGAGCAAGTTTA ACCGCGCCACCCAGGCACTGCGTCAGGTGGGTTCCAACATCAAACCGTTCCTCTACACCGCGGCGATGGATAAAGGTCTG ACGCTGGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAGGTGCCGGTTCTGACTGGCAGCCGAAGAACTC ACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGTGCAATGC GGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAACGCTTCGGCTTCCCGGCGCAAAACATTGTCCACACCGAATCG CTGGCGCTGGGTTCAGCGTCCTTCACCCCAATGCAGGTGGCGCGCGGCTACGCGGTCATGGCGAACGGCGGCTTCCTAGT GGACCCGTGGTTTATCAGCAAAATTGAAAACGATCAGGGCGGCGTGATTTTCGAAGCGAAACCGAAAGTAGCCTGCCCGG AATGCGATATTCCAGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCTATC TCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGC GCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTCCTGATTAAGAGTGCTTTGAACACCAATATCTTTGGTG AACCAGGCTGGCAGGGTACCGGCTGGCGTGCAGGTCGTGATTTGCAGCGTCGCGATATCGGCGGGAAAACCGGTACCACT AACAGTTCGAAAGATGCGTGGTTCTCGGGTTACGGTCCGGGCGTTGTGACCTCGGTCTGGATTGGCTTTGATGATCACCG TCGTAATCTCGGTCATACAACGGCTTCCGGAGCGATTAAAGATCAGATCTCAGGTTACGAAGGCGGTGCCAAGAGTGCCC AGCCTGCATGGGACGCTTATATGAAAGCCGTTCTTGAAGGTGTGCCGGAGCAGCCGCTGACGCCGCCTCCGGGTATTGTG ACGGTGAATATCGATCGCAGCACCGGGCAGTTAGCTAATGGTGGCAACAGCCGCGAAGAGTATTTCATCGAAGGTACGCA GCCGACACAACAGGCGGTGCACGAGGTGGGAACGACCATTATCGATAATGGTGAGGCACAGGAATTGTTCTGA
Upstream 100 bases:
>100_bases CCCGTCACTCTGACGGGTATATCAATGCGTCTGGCTTGCCTTTATACTACCGCGCGTTTGTTTATAAACTGCCCAAATGA AACTAAATGGGAAATTTCCA
Downstream 100 bases:
>100_bases TTAAAAAGGCGCTTCGGCGCCTTTTCAGTTGGCTGACAAAGTGCATTTGTTTATGCCGGATGCGGGTGAACGCGTTATCC GGCTAACAAAATCGTGAAAA
Product: peptidoglycan synthetase
Products: NA
Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]
Number of amino acids: Translated: 850; Mature: 850
Protein sequence:
>850_residues MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRLQIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVK AFIATEDSRFYEHHGVDPVGIFRAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRAVARRNVVLSRMLDEGYITQQQF DQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRYGESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPA NVLWKVGESAWDNNKITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQGPTPRKVTD VLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQSKFNRATQALRQVGSNIKPFLYTAAMDKGL TLASMLNDVPISRWDAGAGSDWQPKNSPPQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTES LALGSASFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDTQKSNVLENNDVEDVAI SREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFLIKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTT NSSKDAWFSGYGPGVVTSVWIGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPGIV TVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF
Sequences:
>Translated_850_residues MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRLQIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVK AFIATEDSRFYEHHGVDPVGIFRAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRAVARRNVVLSRMLDEGYITQQQF DQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRYGESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPA NVLWKVGESAWDNNKITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQGPTPRKVTD VLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQSKFNRATQALRQVGSNIKPFLYTAAMDKGL TLASMLNDVPISRWDAGAGSDWQPKNSPPQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTES LALGSASFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDTQKSNVLENNDVEDVAI SREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFLIKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTT NSSKDAWFSGYGPGVVTSVWIGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPGIV TVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF >Mature_850_residues MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRLQIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVK AFIATEDSRFYEHHGVDPVGIFRAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRAVARRNVVLSRMLDEGYITQQQF DQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRYGESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPA NVLWKVGESAWDNNKITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQGPTPRKVTD VLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQSKFNRATQALRQVGSNIKPFLYTAAMDKGL TLASMLNDVPISRWDAGAGSDWQPKNSPPQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTES LALGSASFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDTQKSNVLENNDVEDVAI SREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFLIKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTT NSSKDAWFSGYGPGVVTSVWIGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPGIV TVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF
Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal
COG id: COG5009
COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein
Gene ontology:
Cell location: Cell inner membrane; Single-pass type II membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI87082258, Length=850, Percent_Identity=99.5294117647059, Blast_Score=1749, Evalue=0.0, Organism=Escherichia coli, GI1786343, Length=251, Percent_Identity=34.6613545816733, Blast_Score=141, Evalue=2e-34, Organism=Escherichia coli, GI1788867, Length=242, Percent_Identity=36.3636363636364, Blast_Score=135, Evalue=1e-32, Organism=Escherichia coli, GI1789601, Length=176, Percent_Identity=37.5, Blast_Score=99, Evalue=1e-21,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001264 - InterPro: IPR011816 - InterPro: IPR001460 [H]
Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]
EC number: 2.4.2.-; 3.4.-.-
Molecular weight: Translated: 93582; Mature: 93582
Theoretical pI: Translated: 6.53; Mature: 6.53
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.5 %Cys (Translated Protein) 2.7 %Met (Translated Protein) 3.2 %Cys+Met (Translated Protein) 0.5 %Cys (Mature Protein) 2.7 %Met (Mature Protein) 3.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRLQIPMQIYSADGELIAQ CHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCEEEECEEEECCCCHHHHH YGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIFRAASVALFSGHASQGAST CCCCCCCCEEHHHCCHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHH ITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEILELYLNKIYLGYRAYGVGAAA HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHH QVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRAVARRNVVLSRMLDEGYITQQQF HEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH DQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRYGESAYEDGYRIYTTITRKVQQAAQ HHHHHHHCCCCCCCCCCEECCHHHHHHHHHHHHHHHCCHHHHCCCEEHHHHHHHHHHHHH QAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNNKITDTLKALPTYGPLLPAAVTSANPQ HHHHCCCCCHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCC GATAMLADGSTVALSMEGVRWARPYRSDTQQGPTPRKVTDVLQTGQQIWVRQVGDAWWLA CCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEE QVPEVNSALVSINPQNGAVMALVGGFDFNQSKFNRATQALRQVGSNIKPFLYTAAMDKGL ECCCCCCEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHCCCC TLASMLNDVPISRWDAGAGSDWQPKNSPPQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDY HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCEEHHHHHHHHCHHH AAEYLQRFGFPAQNIVHTESLALGSASFTPMQVARGYAVMANGGFLVDPWFISKIENDQG HHHHHHHCCCCHHHHEEHHHHCCCCCCCCHHHHHCCEEEEECCCEEECCHHHHHCCCCCC GVIFEAKPKVACPECDIPVIYGDTQKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQA CEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHCCHH LVAKTGAQEYAPHVINTPLAFLIKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTT HHHHCCHHHHCCHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCC NSSKDAWFSGYGPGVVTSVWIGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAY CCCCCCCCCCCCCCCCEEEEECCHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHH MKAVLEGVPEQPLTPPPGIVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTI HHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCEE IDNGEAQELF ECCCCHHCCC >Mature Secondary Structure MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRLQIPMQIYSADGELIAQ CHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCEEEECEEEECCCCHHHHH YGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIFRAASVALFSGHASQGAST CCCCCCCCEEHHHCCHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHH ITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEILELYLNKIYLGYRAYGVGAAA HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHH QVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRAVARRNVVLSRMLDEGYITQQQF HEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH DQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRYGESAYEDGYRIYTTITRKVQQAAQ HHHHHHHCCCCCCCCCCEECCHHHHHHHHHHHHHHHCCHHHHCCCEEHHHHHHHHHHHHH QAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNNKITDTLKALPTYGPLLPAAVTSANPQ HHHHCCCCCHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCC GATAMLADGSTVALSMEGVRWARPYRSDTQQGPTPRKVTDVLQTGQQIWVRQVGDAWWLA CCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEE QVPEVNSALVSINPQNGAVMALVGGFDFNQSKFNRATQALRQVGSNIKPFLYTAAMDKGL ECCCCCCEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHCCCC TLASMLNDVPISRWDAGAGSDWQPKNSPPQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDY HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCEEHHHHHHHHCHHH AAEYLQRFGFPAQNIVHTESLALGSASFTPMQVARGYAVMANGGFLVDPWFISKIENDQG HHHHHHHCCCCHHHHEEHHHHCCCCCCCCHHHHHCCEEEEECCCEEECCHHHHHCCCCCC GVIFEAKPKVACPECDIPVIYGDTQKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQA CEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHCCHH LVAKTGAQEYAPHVINTPLAFLIKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTT HHHHCCHHHHCCHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCC NSSKDAWFSGYGPGVVTSVWIGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAY CCCCCCCCCCCCCCCCEEEEECCHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHH MKAVLEGVPEQPLTPPPGIVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTI HHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCEE IDNGEAQELF ECCCCHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 3882429; 9278503; 3920658; 7006606; 9841666 [H]