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The map label for this gene is dgkA

Identifier: 30064632

GI number: 30064632

Start: 3438485

End: 3438853

Strand: Direct

Name: dgkA

Synonym: S3568

Alternate gene names: 30064632

Gene position: 3438485-3438853 (Clockwise)

Preceding gene: 30064630

Following gene: 30064633

Centisome position: 74.76

GC content: 51.76

Gene sequence:

>369_bases
ATGGCCAATAATACAACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGAT
CAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCAA
TTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGTGGTT
GACCGAATTGGCTCTGAATACCATGAGCTTTCCGGACGCGCAAAAGATATGGGATCCGCCGCGGTGCTGATTGCCATTAT
CGTCGCCGTGATTACCTGGTGCATCCTGTTATGGTCGCATTTTGGATAA

Upstream 100 bases:

>100_bases
GTGGTATCCGCTCATGTTTCGCGCGGCGTTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCAT
AATGTAAAAAGGTTCTTTCA

Downstream 100 bases:

>100_bases
CCCTTCCAGATTTCGATAAATCTCTGGTTTATTGTGCAGTTTATGGTTCCAAAATCGCCTTTTGCTGTATATACTCACAG
TATAACTGTATATACACCCA

Product: diacylglycerol kinase

Products: NA

Alternate protein names: DAGK; Diglyceride kinase; DGK

Number of amino acids: Translated: 122; Mature: 121

Protein sequence:

>122_residues
MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVVIACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVV
DRIGSEYHELSGRAKDMGSAAVLIAIIVAVITWCILLWSHFG

Sequences:

>Translated_122_residues
MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVVIACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVV
DRIGSEYHELSGRAKDMGSAAVLIAIIVAVITWCILLWSHFG
>Mature_121_residues
ANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVVIACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVD
RIGSEYHELSGRAKDMGSAAVLIAIIVAVITWCILLWSHFG

Specific function: Recycling of diacylglycerol produced during the turnover of membrane phospholipid

COG id: COG0818

COG function: function code M; Diacylglycerol kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial diacylglycerol kinase family

Homologues:

Organism=Escherichia coli, GI1790475, Length=122, Percent_Identity=100, Blast_Score=239, Evalue=5e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KDGL_ECO57 (P0ABN3)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A91257
- PIR:   E86097
- RefSeq:   NP_290676.1
- RefSeq:   NP_313052.1
- ProteinModelPortal:   P0ABN3
- EnsemblBacteria:   EBESCT00000025699
- EnsemblBacteria:   EBESCT00000059855
- GeneID:   914310
- GeneID:   960081
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5641
- KEGG:   ecs:ECs5025
- GeneTree:   EBGT00050000011314
- HOGENOM:   HBG729542
- OMA:   ATINSWR
- ProtClustDB:   CLSK879806
- BioCyc:   ECOL83334:ECS5025-MONOMER
- InterPro:   IPR000829

Pfam domain/function: PF01219 DAGK_prokar

EC number: =2.7.1.107

Molecular weight: Translated: 13245; Mature: 13114

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS01069 DAGK_PROKAR

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x100e3110)-; HASH(0x156dcd34)-; HASH(0x15527584)-;

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVVIACWLDVDAITRVLLI
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
SSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAVLIAIIVAVITWCILLWSH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FG
CH
>Mature Secondary Structure 
ANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVVIACWLDVDAITRVLLI
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
SSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAVLIAIIVAVITWCILLWSH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FG
CH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796