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The map label for this gene is hyfE

Identifier: 30063862

GI number: 30063862

Start: 2572452

End: 2573102

Strand: Direct

Name: hyfE

Synonym: S2678

Alternate gene names: 30063862

Gene position: 2572452-2573102 (Clockwise)

Preceding gene: 30063861

Following gene: 30063863

Centisome position: 55.93

GC content: 53.3

Gene sequence:

>651_bases
ATGACCGGTTCTATGATCGTAAATAATCTGGCGGGACTGATGATGCTGACATCGCTGTTTGTGATTAGCGTCAAAAGCTA
TCGCCTGTCATGCGGATTTTACGCCTGCCAGTCGCTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCG
CAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTGCTGGTACCGTTAATCATGACTTACGCTGCA
CGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTCGCGGCGTTAATTGTCCT
GCTTTGCGCATTTGTCGTTCAGCCCGTGAAGCTACCGATGGCTACCGGGCTGAAACCGGCGCTGGCGGTAGCGTTAGGTC
ATTTTCTGCTTGGCCTGCTGTGCATTGTCAGCCAACGCAATATCCTGCGGCAAATTTTTGGTTACTGCCTGATGGAAAAC
GGCTCCCATCTGGTGCTGGCGCTTCTTGCCTGGCGAGCACCGGAACTGGTGGAAATAGGTATCGCTACCGACGCCATCTT
CGCCGTCATTGTGATGGTGTTACTGGCAAGAAAAATATGGCGTACCCACGGCACGCTGGACGTGAACAACTTGACCGCGC
TGAAGGGATAA

Upstream 100 bases:

>100_bases
CGCTGCCAGGGTCAATGCGCCTGGTGTTGATTGTACTGATTGTGATGTCGCTGATTTCCAGCGTAATCGCCGCGACCTGG
TTGCAGTAAGGAGATGATGA

Downstream 100 bases:

>100_bases
TGAGATGAGTTATTCTGTGATGTTCGCTTTACTCCTGCTCACGCCGCTGCTTTTTTCGCTGCTCTGTTTTGCCTGCCGGA
AACGGGGGCTCTCTGCGACT

Product: hydrogenase 4 membrane subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSIFATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAA
RNIPQNIPEKALFGPAMMALLAALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG

Sequences:

>Translated_216_residues
MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSIFATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAA
RNIPQNIPEKALFGPAMMALLAALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG
>Mature_215_residues
TGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSIFATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAAR
NIPQNIPEKALFGPAMMALLAALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMENG
SHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG

Specific function: Unknown

COG id: COG4237

COG function: function code C; Hydrogenase 4 membrane component (E)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788830, Length=216, Percent_Identity=100, Blast_Score=429, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HYFE_ECO57 (P0AEW2)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C91047
- PIR:   G85891
- RefSeq:   NP_289038.1
- RefSeq:   NP_311374.1
- ProteinModelPortal:   P0AEW2
- EnsemblBacteria:   EBESCT00000026352
- EnsemblBacteria:   EBESCT00000056918
- GeneID:   915241
- GeneID:   957815
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3745
- KEGG:   ecs:ECs3347
- GeneTree:   EBGT00050000009042
- HOGENOM:   HBG393381
- OMA:   FENGIHL
- ProtClustDB:   PRK11492
- BioCyc:   ECOL83334:ECS3347-MONOMER
- InterPro:   IPR001133

Pfam domain/function: PF00420 Oxidored_q2

EC number: 1.-.-.- [C]

Molecular weight: Translated: 23362; Mature: 23231

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x138f9a98)-; HASH(0x142bf8ac)-; HASH(0x1357f9d4)-; HASH(0x14357304)-; HASH(0x14265904)-; HASH(0x142c3c0c)-; HASH(0x133304a0)-;

Cys/Met content:

2.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSIFATLSCLFAAEQLLIW
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLAALIVLLCAFVVQPVKLPM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMENGSHLVLALLAWRAPELVEIG
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHH
IATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
>Mature Secondary Structure 
TGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSIFATLSCLFAAEQLLIW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLAALIVLLCAFVVQPVKLPM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMENGSHLVLALLAWRAPELVEIG
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHH
IATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796