Click here to switch to the map view.

The map label for this gene is hyfD

Identifier: 30063861

GI number: 30063861

Start: 2571001

End: 2572440

Strand: Direct

Name: hyfD

Synonym: S2677

Alternate gene names: 30063861

Gene position: 2571001-2572440 (Clockwise)

Preceding gene: 30063860

Following gene: 30063862

Centisome position: 55.9

GC content: 55.56

Gene sequence:

>1440_bases
ATGGAAAATCTTGCTCTGACGACGTTAATGCTGCCTTTTATCGGCGCACTGGTCGTTTCGTTTTCGCCACAACGTCGGGC
CGCCGAATGGGGGGTTTTGTTCGCCGCGCTGACCACGCTGTGCATGTTGTCGCTGATCTCCGCGTTTTATCAGGCCGATA
AAGTTGCCGTCACGTTGACGTTGGTCAACGTGGGGGATGTGGCATTATTTGGCCTGGTCATTGATCGCGTGAGTACGCTG
ATTCTGTTTGTGGTGGTGTTTCTCGGTTTGCTGGTCACGATCTACTCCACGGGTTATCTGACGGATAAAAATCGCGAACA
CCCGCATAACGGCACGAATCGTTATTACGCATTTTTGCTGGTGTTTATCGGCGCGATGGCGGGACTGGTACTCTCCTCGA
CGCTGCTCGGTCAGTTGTTGTTTTTTGAAATTACGGGCGGCTGCTCCTGGGCGTTGATCAGTTATTACCAGAGCGATAAA
GCGCAGCGTTCAGCACTAAAAGCGTTGCTTATCACCCATATCGGTTCGTTGGGGTTGTATCTTGCCGCCGCCACGCTGTT
TTTGCAGACCGGAACGTTTGTGCTTAGCGCGATGAGCGAGTTACATGGCGACGCACGTTATCTGGTTTATGGCGGCATCC
TGTTTGCCGCGTGGGGGAAATCGGCCCAGCTACCGATGCAAGCGTGGCTACCGGACGCAATGGAAGCGCCAACACCGATC
AGCGCCTATCTCCACGCCGCATCGATGGTGAAAGTGGGCGTTTACATTTTTGCCCGCGCCATTATCGACGGCGGCAATAT
CCCGCATGTGATTGGCGGCGTTGGCATGGTCATGACACTGGTCACCATTCTTTATGGCTTTCTGATGTATTTGCCACAGC
AGGATATGAAGCGGTTGCTGGCCTGGTCGACCATCACTCAACTTGGCTGGATGTTCTTCGGCTTGTCGCTCTCCATCTTC
GGCTCGCGGCTGGCGCTGGAGGGTAGCATCGCCTACATCGTCAACCACGCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGC
AGGTGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCG
TGGGTTTCTGCGTGGCAGCGCTGGCAATTACCGGTGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCC
GCCGGTTTTGCGTTGTCAGTGGAGTACTGGATCCTGCTGCCCGCCATGATTCTGCTGATGATTGAATCGGTCGCCAGTTT
CGCCTGGTTTATTCGCTGGTTTGGTCGCGTCGTGCCGGGCAAACCGAGCGAGGCCGTCGCCGATGCCGCACCGCTGCCAG
GGTCAATGCGCCTGGTGTTGATTGTACTGATTGTGATGTCGCTGATTTCCAGCGTAATCGCCGCGACCTGGTTGCAGTAA

Upstream 100 bases:

>100_bases
CGTTTTTTACTCATTCACCATGTGACCTGGCTTGGCTTCAGCCTTGCTGCGCTTGCATGGGTCTTTTGGTTAACCGGTCT
GTAAGGAGCACTGACGGAAT

Downstream 100 bases:

>100_bases
GGAGATGATGAATGACCGGTTCTATGATCGTAAATAATCTGGCGGGACTGATGATGCTGACATCGCTGTTTGTGATTAGC
GTCAAAAGCTATCGCCTGTC

Product: hydrogenase 4 subunit D

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLSLISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTL
ILFVVVFLGLLVTIYSTGYLTDKNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAWGKSAQLPMQAWLPDAMEAPTPI
SAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGMVMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIF
GSRLALEGSIAYIVNHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGFFSKFPLFA
AGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAAPLPGSMRLVLIVLIVMSLISSVIAATWLQ

Sequences:

>Translated_479_residues
MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLSLISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTL
ILFVVVFLGLLVTIYSTGYLTDKNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAWGKSAQLPMQAWLPDAMEAPTPI
SAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGMVMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIF
GSRLALEGSIAYIVNHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGFFSKFPLFA
AGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAAPLPGSMRLVLIVLIVMSLISSVIAATWLQ
>Mature_479_residues
MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLSLISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTL
ILFVVVFLGLLVTIYSTGYLTDKNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAWGKSAQLPMQAWLPDAMEAPTPI
SAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGMVMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIF
GSRLALEGSIAYIVNHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGFFSKFPLFA
AGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAAPLPGSMRLVLIVLIVMSLISSVIAATWLQ

Specific function: Unknown

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=325, Percent_Identity=31.3846153846154, Blast_Score=135, Evalue=8e-32,
Organism=Escherichia coli, GI1788829, Length=479, Percent_Identity=99.3736951983299, Blast_Score=946, Evalue=0.0,
Organism=Escherichia coli, GI1788614, Length=421, Percent_Identity=33.729216152019, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1788827, Length=435, Percent_Identity=24.8275862068966, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1788831, Length=433, Percent_Identity=29.7921478060046, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI2367154, Length=191, Percent_Identity=27.7486910994764, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI145693160, Length=331, Percent_Identity=24.4712990936556, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788613, Length=234, Percent_Identity=26.4957264957265, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 51831; Mature: 51831

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]