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The map label for this gene is insA [H]

Identifier: 30063840

GI number: 30063840

Start: 2538967

End: 2539239

Strand: Reverse

Name: insA [H]

Synonym: S2651

Alternate gene names: 30063840

Gene position: 2539239-2538967 (Counterclockwise)

Preceding gene: 30063841

Following gene: 30063839

Centisome position: 55.21

GC content: 48.72

Gene sequence:

>273_bases
ATGACCAGCGTTAATATTCATTGTCCCCGTTGTCAGTCAGCTCAGGTTTACCGCCATGGTCAGAACCCTAAAGGCCGTGA
CAGACTTCGCTGCCGTGACTGCCACCGTGTGTTTCAGTTCACTTATACTTATCAAGCACGTAAGCCGGGTATGAAAGAGC
TGATAACTGAAATGGCCTTTAATGGTGCCGGGGTTCGCGATACCGCCAGGACACTGAAAATTGGTAGTAACACCGTCATC
CGGACTTTAAAAAACTCGCGCCAAAGCGAATAA

Upstream 100 bases:

>100_bases
ATTATAATTTAGTTATTGTTAGTATGTTTTATAATGCTGGATGGGTGATGCCTCTAATTAGTTGAATCTGATGTATAATA
CGGGCTTTTGAGGTTATCTC

Downstream 100 bases:

>100_bases
CTTCTTCGCCTGTTGCCCATGCTGATGTGGCGTTTATCTGCGAGCTTGATGAGCAATGGAGCTACGTTGGCAGTAAAGCC
CGGCAACATTGGCTCGGGTA

Product: IS1N orfA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 90; Mature: 89

Protein sequence:

>90_residues
MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTYQARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVI
RTLKNSRQSE

Sequences:

>Translated_90_residues
MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTYQARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVI
RTLKNSRQSE
>Mature_89_residues
TSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTYQARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIR
TLKNSRQSE

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG3677

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS1 elements insA family [H]

Homologues:

Organism=Escherichia coli, GI1789852, Length=90, Percent_Identity=43.3333333333333, Blast_Score=82, Evalue=6e-18,
Organism=Escherichia coli, GI1788204, Length=90, Percent_Identity=43.3333333333333, Blast_Score=82, Evalue=6e-18,
Organism=Escherichia coli, GI1786204, Length=90, Percent_Identity=43.3333333333333, Blast_Score=82, Evalue=6e-18,
Organism=Escherichia coli, GI1790747, Length=90, Percent_Identity=42.2222222222222, Blast_Score=81, Evalue=1e-17,
Organism=Escherichia coli, GI87081814, Length=90, Percent_Identity=43.3333333333333, Blast_Score=81, Evalue=1e-17,
Organism=Escherichia coli, GI1786471, Length=90, Percent_Identity=43.3333333333333, Blast_Score=81, Evalue=1e-17,
Organism=Escherichia coli, GI1786460, Length=90, Percent_Identity=43.3333333333333, Blast_Score=81, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003220 [H]

Pfam domain/function: PF03811 Ins_element1 [H]

EC number: NA

Molecular weight: Translated: 10388; Mature: 10257

Theoretical pI: Translated: 10.98; Mature: 10.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTYQARKPGMKELITEMAF
CCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
NGAGVRDTARTLKIGSNTVIRTLKNSRQSE
CCCCCCCCHHEEECCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
TSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTYQARKPGMKELITEMAF
CCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHC
NGAGVRDTARTLKIGSNTVIRTLKNSRQSE
CCCCCCCCHHEEECCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6265806 [H]