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The map label for this gene is insB [H]

Identifier: 30063839

GI number: 30063839

Start: 2538545

End: 2538940

Strand: Reverse

Name: insB [H]

Synonym: S2650

Alternate gene names: 30063839

Gene position: 2538940-2538545 (Counterclockwise)

Preceding gene: 30063840

Following gene: 30063830

Centisome position: 55.2

GC content: 51.77

Gene sequence:

>396_bases
GTGGCGTTTATCTGCGAGCTTGATGAGCAATGGAGCTACGTTGGCAGTAAAGCCCGGCAACATTGGCTCGGGTACGCGTA
CAACACCAAAACAGGCGGTGTACTGGCCTACACTTTTGGTCCCCGAACCGATCAAACGTGCCGGGAGCTACTGGCACTGC
TTACACCCTTCAACATCGGCATGCTGACCAGCGATGACTGGGGCAGCTATGGCCGGGAGGTGCCGAAGAATAAGCATCTG
ACCGGCAAAATATTCACCCAACGCATTGAGCGTAATAATCTGACGCTACGCACCCGCATTAAGCGGTTGGGTCGTAAAAC
AATCTGCTTCTCACGCTCAGTTGAGATCCACGAAAAAGTGATCGGAGCGTTTATCGAAAAACACATGTTCTACTAA

Upstream 100 bases:

>100_bases
ATACCGCCAGGACACTGAAAATTGGTAGTAACACCGTCATCCGGACTTTAAAAAACTCGCGCCAAAGCGAATAACTTCTT
CGCCTGTTGCCCATGCTGAT

Downstream 100 bases:

>100_bases
TTGGATGCACTACCCAGTATGTAACTGGGTTTGTCCATAAAATGCACCTTACTCAGTTGGTGTCCAACTTTTGGGGTGCA
GTTCAGAAGGACGGGATTTT

Product: iso-IS1 ORF2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 131; Mature: 130

Protein sequence:

>131_residues
MAFICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIGMLTSDDWGSYGREVPKNKHL
TGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHEKVIGAFIEKHMFY

Sequences:

>Translated_131_residues
MAFICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIGMLTSDDWGSYGREVPKNKHL
TGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHEKVIGAFIEKHMFY
>Mature_130_residues
AFICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIGMLTSDDWGSYGREVPKNKHLT
GKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHEKVIGAFIEKHMFY

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=125, Percent_Identity=47.2, Blast_Score=127, Evalue=3e-31,
Organism=Escherichia coli, GI1788203, Length=125, Percent_Identity=47.2, Blast_Score=127, Evalue=3e-31,
Organism=Escherichia coli, GI1786203, Length=125, Percent_Identity=47.2, Blast_Score=127, Evalue=3e-31,
Organism=Escherichia coli, GI1787223, Length=125, Percent_Identity=47.2, Blast_Score=124, Evalue=2e-30,
Organism=Escherichia coli, GI1786470, Length=125, Percent_Identity=47.2, Blast_Score=124, Evalue=2e-30,
Organism=Escherichia coli, GI1786459, Length=125, Percent_Identity=47.2, Blast_Score=124, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 15239; Mature: 15108

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
CCEEECCCHHHHHHHHHHHHHHHCEEEECCCCCEEEEEECCCCHHHHHHHHHHHCCCCCC
MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHEKV
EEECCCCCHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHCCCHHHHHHH
IGAFIEKHMFY
HHHHHHHHCCC
>Mature Secondary Structure 
AFICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
CEEECCCHHHHHHHHHHHHHHHCEEEECCCCCEEEEEECCCCHHHHHHHHHHHCCCCCC
MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHEKV
EEECCCCCHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHCCCHHHHHHH
IGAFIEKHMFY
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6265806 [H]