Click here to switch to the map view.

The map label for this gene is relE

Identifier: 30063050

GI number: 30063050

Start: 1620096

End: 1620383

Strand: Reverse

Name: relE

Synonym: S1669

Alternate gene names: 30063050

Gene position: 1620383-1620096 (Counterclockwise)

Preceding gene: 30063051

Following gene: 30063049

Centisome position: 35.23

GC content: 46.88

Gene sequence:

>288_bases
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Upstream 100 bases:

>100_bases
AACAGACACTCCTGAGTGATGAAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGT
GTGACGCTGGATGAACTCTG

Downstream 100 bases:

>100_bases
ACCAAAGCATGACATCTCTGTTTCGCACCGAAGGTGACACTTCTGCTTTGCGTTGACAGGAGAAGCAGGCTATGAAGCAG
CAAAAGGCGATGTTAATCGC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 95; Mature: 94

Protein sequence:

>95_residues
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Sequences:

>Translated_95_residues
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL
>Mature_94_residues
AYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKR
ERSEVYSEAVKRIL

Specific function: Inhibits translation during amino acid starvation. Acts by cleaving mRNA with high codon specificity in the ribosomal A site. UGA is cleaved with fast rate while UAA and UGA are cleaved with intermediate and slow rate, respectively. mRNA cleavage can also

COG id: COG2026

COG function: function code JD; Cytotoxic translational repressor of toxin-antitoxin stability system

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787846, Length=95, Percent_Identity=100, Blast_Score=188, Evalue=6e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RELE_ECOLI (P0C077)

Other databases:

- EMBL:   X02405
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   B22830
- RefSeq:   AP_002185.1
- RefSeq:   NP_416081.1
- PDB:   2KC8
- PDB:   2KC9
- PDB:   3KHA
- PDB:   3KIQ
- PDB:   3KIS
- PDB:   3KIU
- PDB:   3KIX
- PDBsum:   2KC8
- PDBsum:   2KC9
- PDBsum:   3KHA
- PDBsum:   3KIQ
- PDBsum:   3KIS
- PDBsum:   3KIU
- PDBsum:   3KIX
- ProteinModelPortal:   P0C077
- SMR:   P0C077
- DIP:   DIP-35978N
- MINT:   MINT-1282331
- STRING:   P0C077
- EnsemblBacteria:   EBESCT00000000003
- EnsemblBacteria:   EBESCT00000015154
- GeneID:   947549
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1555
- KEGG:   eco:b1563
- EchoBASE:   EB1121
- EcoGene:   EG11131
- eggNOG:   COG2026
- GeneTree:   EBGT00050000012059
- HOGENOM:   HBG705334
- OMA:   NIDFDER
- ProtClustDB:   CLSK891757
- BioCyc:   EcoCyc:EG11131-MONOMER
- Genevestigator:   P0C077
- GO:   GO:0009405
- GO:   GO:0006350
- InterPro:   IPR007712
- InterPro:   IPR012753
- TIGRFAMs:   TIGR02385

Pfam domain/function: PF05016 Plasmid_stabil

EC number: NA

Molecular weight: Translated: 11225; Mature: 11094

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGY
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCEEEEECCCCH
RLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL
HHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHC
>Mature Secondary Structure 
AYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGY
CEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCEEEEECCCCH
RLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL
HHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2990907; 9097039; 9278503; 9767574; 12526800