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The map label for this gene is relF

Identifier: 30063049

GI number: 30063049

Start: 1619869

End: 1620024

Strand: Reverse

Name: relF

Synonym: S1668

Alternate gene names: 30063049

Gene position: 1620024-1619869 (Counterclockwise)

Preceding gene: 30063050

Following gene: 30063048

Centisome position: 35.22

GC content: 51.92

Gene sequence:

>156_bases
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Upstream 100 bases:

>100_bases
ATAGCGAGGCGGTCAAACGCATTCTCTGAACCAAAGCATGACATCTCTGTTTCGCACCGAAGGTGACACTTCTGCTTTGC
GTTGACAGGAGAAGCAGGCT

Downstream 100 bases:

>100_bases
GAGACCCGGCGAGTGAGAAATCCCTCGCCACCTCTGATGTGGCAGGCATCCTCAACGCACCCGCACTTAACCCGCTTCGG
CGGGTTTTTGTTTTTATTTT

Product: prophage maintenance protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 51; Mature: 51

Protein sequence:

>51_residues
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Sequences:

>Translated_51_residues
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE
>Mature_51_residues
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Specific function: When Overexpressed Kill The Cells From The Inside By Interfering With A VItal Function In The Cell Membrane. Overexpression Of Relf Results In Collapse Of The Transmembrane Potential And Concomitant Arrest Of Oxygen Consumption. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hok/gef family

Homologues:

Organism=Escherichia coli, GI1787845, Length=51, Percent_Identity=100, Blast_Score=101, Evalue=1e-23,
Organism=Escherichia coli, GI48994874, Length=50, Percent_Identity=82, Blast_Score=87, Evalue=2e-19,
Organism=Escherichia coli, GI1786200, Length=50, Percent_Identity=82, Blast_Score=86, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOKD_ECO57 (P0ACG7)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B90973
- RefSeq:   NP_310781.1
- ProteinModelPortal:   P0ACG7
- EnsemblBacteria:   EBESCT00000025421
- EnsemblBacteria:   EBESCT00000056086
- GeneID:   912381
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ecs:ECs2754
- GeneTree:   EBGT00050000009513
- HOGENOM:   HBG473224
- ProtClustDB:   CLSK512088
- BioCyc:   ECOL83334:ECS2754-MONOMER
- InterPro:   IPR000021
- InterPro:   IPR018084
- PRINTS:   PR00281
- ProDom:   PD005979

Pfam domain/function: PF01848 HOK_GEF

EC number: NA

Molecular weight: Translated: 5737; Mature: 5737

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS00556 HOK_GEF

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x12b37204)-;

Cys/Met content:

3.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCH
>Mature Secondary Structure
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796