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The map label for this gene is rlmL

Identifier: 30062485

GI number: 30062485

Start: 998876

End: 1000984

Strand: Direct

Name: rlmL

Synonym: S1014

Alternate gene names: 30062485

Gene position: 998876-1000984 (Clockwise)

Preceding gene: 30062483

Following gene: 30062486

Centisome position: 21.72

GC content: 53.96

Gene sequence:

>2109_bases
ATGAATTCTCTGTTTGCCAGTACGGCCCGTGGGCTGGAAGAGCTGTTAAAAACTGAACTGGAAAATCTGGGGGCCGTTGA
ATGCCAGGTGGTTCAGGGTGGGGTCCATTTCAAGGGCGACACACGGCTTGTTTACCAGAGCCTGATGTGGAGCCGCCTGG
CCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAAC
TGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATGACACCATCCGCAACAGTCAGTA
CGGCGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACACGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATG
CGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACCGCCAGTATCGCTCTTGATCTGAGTGGTGATGGTTTACAT
CTGCGTGGCTATCGCGATCGTGCTGGTATTGCGCCGATCAAAGAAACCCTGGCAGCCGCTATTGTGATGCGATCCGGCTG
GCAGTCAGGAACACCGCTGCTCGATCCGATGTGTGGTTCCGGTACGTTGCTGATTGAAGCGGCGATGCTGGCGACCGATC
GCGCACCAGGCTTGCACCGTGGGCGTTGGGGCTTTAGCGGCTGGGCGCAGCATGATGAAGCTATCTGGCAGGAAGTGAAA
GCGGAAGCGCAAACTCGCGCCCGTAAAGGCCTAGCTGAGTATAGCTCTCATTTTTACGGTTCGGACAGCGACGCACGGGT
GATTCAACGTGCGCGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAGATGTCGCGCAAC
TGACCAATCCGCTGCCGAAAGGGCCGTACGGTACAGTGTTGAGCAACCCGCCATACGGTGAACGTCTGGACAGCGAACCG
GCGCTGATTGCGCTGCATAGTCTGCTGGGCCGAATCATGAAAAACCAGTTCGGTGGCTGGAATCTCTCTTTGTTTAGTGC
CTCGCCGGATCTGCTAAGCTGCCTGCAGCTGCGTGCAGACAAACAGTACAAGGCGAAAAACGGCCCGCTGGACTGCGTAC
AGAAAAATTACCACGTTGCCGAAAGCACACCAGACAGTAAACCGGCAATGGTAGCGGAAGATTACGCCAACCGCCTGCGT
AAGAACCTCAAAAAATTCGAGAAGTGGGCACGCCAGGAAGGGATTGAATGTTACCGCCTGTATGACGCCGATCTGCCGGA
ATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCGCACA
AAGCGCGTCAGCGTCTGTTCGATATTATCGCTGCAACCATTTCGGTACTGGGGATTGCGCCAAACAAACTGGTGCTGAAA
ACCCGTGAACGCCATAAGGGCAAAAACCAGTACCAGAAACTGGGCGAGAAGGGCGAGTTTCTTGAAGTTACCGAATATAA
CGCTCACTTGTGGGTGAACCTGACGGATTATCTCGATACTGGTCTGTTCCTCGATCACCGTATCGCCCGCCGTATGCTCG
GTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTTTCTTATACCGGCAGCGCCACCGTGCATGCGGGATTAGGCGGT
GCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAATGGGCAGAACGCAACCTGCGTCTGAATGGCCTGAC
TAGGCGTGCGCATCGCCTGATTCAGGCCGATTGCCTGGCGTGGCTGCGTGAGGCAAATGAACAGTTCGATCTGATCTTTA
TCGATCCGCCAACCTTCTCTAACTCAAAACGAATGGAAGATGCGTTTGATGTTCAGCGCGATCATCTGGCGCTGATGAAA
GATTTGAAACGTCTGCTGCGTGCAGGTGGGACGATCATGTTCTCGAACAACAAACGTGGCTTCCGTATGGATCTCGACGG
CCTGGCAAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAACGCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCC
ACAACTGCTGGCTGATTACCGCAGCCTGA

Upstream 100 bases:

>100_bases
ACGTACTTGTGTACGCTCCGGTTTCTCCGCGCTCTCCATGTCCAGACTGACTGCAACAATTACGCCTACTGCGGTAGGCT
CTTAGAGTAAAAGTGACGAT

Downstream 100 bases:

>100_bases
AAGGAATAGTAATGTCATTAATCAGTATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAA
CTGCATATCGAAGATAACGA

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 702; Mature: 702

Protein sequence:

>702_residues
MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLVYQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAIN
WTEMFNPGATFAVHFSGLNDTIRNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGLHRGRWGFSGWAQHDEAIWQEVK
AEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNARLAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEP
ALIALHSLLGRIMKNQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVAEDYANRLR
KNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTIDAHKARQRLFDIIAATISVLGIAPNKLVLK
TRERHKGKNQYQKLGEKGEFLEVTEYNAHLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGG
ARSTTTVDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKRMEDAFDVQRDHLALMK
DLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKTLSQDFARNRQIHNCWLITAA

Sequences:

>Translated_702_residues
MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLVYQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAIN
WTEMFNPGATFAVHFSGLNDTIRNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGLHRGRWGFSGWAQHDEAIWQEVK
AEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNARLAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEP
ALIALHSLLGRIMKNQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVAEDYANRLR
KNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTIDAHKARQRLFDIIAATISVLGIAPNKLVLK
TRERHKGKNQYQKLGEKGEFLEVTEYNAHLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGG
ARSTTTVDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKRMEDAFDVQRDHLALMK
DLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKTLSQDFARNRQIHNCWLITAA
>Mature_702_residues
MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLVYQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAIN
WTEMFNPGATFAVHFSGLNDTIRNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGLHRGRWGFSGWAQHDEAIWQEVK
AEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNARLAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEP
ALIALHSLLGRIMKNQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVAEDYANRLR
KNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTIDAHKARQRLFDIIAATISVLGIAPNKLVLK
TRERHKGKNQYQKLGEKGEFLEVTEYNAHLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGG
ARSTTTVDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKRMEDAFDVQRDHLALMK
DLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKTLSQDFARNRQIHNCWLITAA

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=702, Percent_Identity=99.4301994301994, Blast_Score=1447, Evalue=0.0,
Organism=Escherichia coli, GI145693111, Length=241, Percent_Identity=29.045643153527, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 78923; Mature: 78923

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLVYQSLMWSRLASRIMLP
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEECCCHHHHHHHHHHHHHHHHHCCC
LGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTIRNSQYGAMKVKDAIVDAF
CCCCEEECCCCEEEEEEEECHHHHCCCCCEEEEEECCCCHHHCCCCCCCHHHHHHHHHHH
TRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLHLRGYRDRAGIAPIKETLAAA
HHCCCCCCCCCCCCCCEEEEEEEEECCEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHH
IVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGLHRGRWGFSGWAQHDEAIWQEVK
HHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNARLAGIGELITFEVKDVAQLTNPLPK
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHCCCCHHEEEEHHHHHHHHCCCCC
GPYGTVLSNPPYGERLDSEPALIALHSLLGRIMKNQFGGWNLSLFSASPDLLSCLQLRAD
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCC
KQYKAKNGPLDCVQKNYHVAESTPDSKPAMVAEDYANRLRKNLKKFEKWARQEGIECYRL
CCCCCCCCCHHHHHCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
YDADLPEYNVAVDRYADWVVVQEYAPPKTIDAHKARQRLFDIIAATISVLGIAPNKLVLK
ECCCCCCCCEEHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
TRERHKGKNQYQKLGEKGEFLEVTEYNAHLWVNLTDYLDTGLFLDHRIARRMLGQMSKGK
CHHHCCCHHHHHHHCCCCCEEEEEECCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHCCH
DFLNLFSYTGSATVHAGLGGARSTTTVDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLA
HHHHHHHCCCCCEEEECCCCCCCCEEEHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHH
WLREANEQFDLIFIDPPTFSNSKRMEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRG
HHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
FRMDLDGLAKLGLKAQEITQKTLSQDFARNRQIHNCWLITAA
EEECHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEC
>Mature Secondary Structure
MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLVYQSLMWSRLASRIMLP
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEECCCHHHHHHHHHHHHHHHHHCCC
LGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTIRNSQYGAMKVKDAIVDAF
CCCCEEECCCCEEEEEEEECHHHHCCCCCEEEEEECCCCHHHCCCCCCCHHHHHHHHHHH
TRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLHLRGYRDRAGIAPIKETLAAA
HHCCCCCCCCCCCCCCEEEEEEEEECCEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHH
IVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGLHRGRWGFSGWAQHDEAIWQEVK
HHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNARLAGIGELITFEVKDVAQLTNPLPK
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHCCCCHHEEEEHHHHHHHHCCCCC
GPYGTVLSNPPYGERLDSEPALIALHSLLGRIMKNQFGGWNLSLFSASPDLLSCLQLRAD
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCC
KQYKAKNGPLDCVQKNYHVAESTPDSKPAMVAEDYANRLRKNLKKFEKWARQEGIECYRL
CCCCCCCCCHHHHHCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
YDADLPEYNVAVDRYADWVVVQEYAPPKTIDAHKARQRLFDIIAATISVLGIAPNKLVLK
ECCCCCCCCEEHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
TRERHKGKNQYQKLGEKGEFLEVTEYNAHLWVNLTDYLDTGLFLDHRIARRMLGQMSKGK
CHHHCCCHHHHHHHCCCCCEEEEEECCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHCCH
DFLNLFSYTGSATVHAGLGGARSTTTVDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLA
HHHHHHHCCCCCEEEECCCCCCCCEEEHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHHH
WLREANEQFDLIFIDPPTFSNSKRMEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRG
HHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
FRMDLDGLAKLGLKAQEITQKTLSQDFARNRQIHNCWLITAA
EEECHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA