Click here to switch to the map view.

The map label for this gene is insB1 [H]

Identifier: 30062474

GI number: 30062474

Start: 988345

End: 989040

Strand: Direct

Name: insB1 [H]

Synonym: S1002

Alternate gene names: 30062474

Gene position: 988345-989040 (Clockwise)

Preceding gene: 30062467

Following gene: 30062477

Centisome position: 21.49

GC content: 52.87

Gene sequence:

>696_bases
GTGGCTTCCATTTCCATCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACA
TCAGCGCTATCTCTGCTCTCATTGCCGTAAAACATGGCAACTACAGTTCACTTACACCGCCTCTCAGCCCGGTACGCACC
AGAAAATCATTGATATGGCCATGAATGGCGTCGGATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCTACACGGTT
TTACGTCACTTAAAAAACTCAGCCGAGTCGGTAACCTCGCGCATACAACCGGGCAGTGATGTGATTGTCTGCGCTGAAAT
GGACGAACATTGGGGCTACGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGG
TTGTGGCGCACGTCTTCGGGGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTG
GTATGGATGACGGATGGCTGTCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCA
GCGCATTGAGCGACATAATCTGAATCTGAGACAACATCTGGCAAGGCTGGTACGGAAGTCACTGTCGTTCTCAAAATCGG
TGGAGCTGCATGACAAGGCCATCGGGCATTATCTGAACATAAAACACTATCAGTAA

Upstream 100 bases:

>100_bases
ACATTGATAACAATTCGGTCAGTCTGTCGGAGAGACAAGAAAATGGGTAATGCTGCCAACTTACTGATTTAGTGTATGAT
GGTGATTTTAAGGTGCTTGC

Downstream 100 bases:

>100_bases
GTTGGAGTCATTACCTTAATGGTAAAGTTATTGCTCCAGCTTGTACCCTGGTAGCTGCGACGAAAGATTCAGTAGTCACA
TTGCCAAATGTCAGCGCCAC

Product: IS1 orfB, A

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDMAMNGVGCRASARIMGVGLYTV
LRHLKNSAESVTSRIQPGSDVIVCAEMDEHWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVV
VWMTDGCPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLVRKSLSFSKSVELHDKAIGHYLNIKHYQ

Sequences:

>Translated_231_residues
MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDMAMNGVGCRASARIMGVGLYTV
LRHLKNSAESVTSRIQPGSDVIVCAEMDEHWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVV
VWMTDGCPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLVRKSLSFSKSVELHDKAIGHYLNIKHYQ
>Mature_230_residues
ASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDMAMNGVGCRASARIMGVGLYTVL
RHLKNSAESVTSRIQPGSDVIVCAEMDEHWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVV
WMTDGCPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLVRKSLSFSKSVELHDKAIGHYLNIKHYQ

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=154, Percent_Identity=85.7142857142857, Blast_Score=273, Evalue=5e-75,
Organism=Escherichia coli, GI1788203, Length=154, Percent_Identity=85.7142857142857, Blast_Score=273, Evalue=5e-75,
Organism=Escherichia coli, GI1786203, Length=154, Percent_Identity=85.7142857142857, Blast_Score=273, Evalue=5e-75,
Organism=Escherichia coli, GI1787223, Length=147, Percent_Identity=88.4353741496599, Blast_Score=272, Evalue=1e-74,
Organism=Escherichia coli, GI1786470, Length=147, Percent_Identity=88.4353741496599, Blast_Score=271, Evalue=2e-74,
Organism=Escherichia coli, GI1786459, Length=147, Percent_Identity=88.4353741496599, Blast_Score=271, Evalue=2e-74,
Organism=Escherichia coli, GI1790747, Length=90, Percent_Identity=94.4444444444444, Blast_Score=178, Evalue=2e-46,
Organism=Escherichia coli, GI1789852, Length=90, Percent_Identity=91.1111111111111, Blast_Score=177, Evalue=4e-46,
Organism=Escherichia coli, GI1788204, Length=90, Percent_Identity=91.1111111111111, Blast_Score=177, Evalue=4e-46,
Organism=Escherichia coli, GI1786204, Length=90, Percent_Identity=91.1111111111111, Blast_Score=177, Evalue=4e-46,
Organism=Escherichia coli, GI87081814, Length=90, Percent_Identity=90, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1786471, Length=90, Percent_Identity=90, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1786460, Length=90, Percent_Identity=90, Blast_Score=176, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 26364; Mature: 26233

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDMA
CCEEEEECCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHH
MNGVGCRASARIMGVGLYTVLRHLKNSAESVTSRIQPGSDVIVCAEMDEHWGYVGAKSRQ
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCC
RWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGCPLYESRLKGKLHV
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHCCCHHHH
ISKRYTQRIERHNLNLRQHLARLVRKSLSFSKSVELHDKAIGHYLNIKHYQ
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEECCC
>Mature Secondary Structure 
ASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDMA
CEEEEECCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHH
MNGVGCRASARIMGVGLYTVLRHLKNSAESVTSRIQPGSDVIVCAEMDEHWGYVGAKSRQ
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCC
RWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGCPLYESRLKGKLHV
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHCCCHHHH
ISKRYTQRIERHNLNLRQHLARLVRKSLSFSKSVELHDKAIGHYLNIKHYQ
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]