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The map label for this gene is phoB

Identifier: 30061895

GI number: 30061895

Start: 345784

End: 346473

Strand: Direct

Name: phoB

Synonym: S0344

Alternate gene names: 30061895

Gene position: 345784-346473 (Clockwise)

Preceding gene: 161486537

Following gene: 30061896

Centisome position: 7.52

GC content: 53.91

Gene sequence:

>690_bases
ATGGCGAGACGTATTCTGGTCGTAGAAGATGAAGCTCCAATTCGCGAAATGGTCTGCTTCGTGCTCGAACAAAATGGCTT
TCAGCCGGTCGAAGCGGAAGATTATGACAGTGCTGTGAATCAACTGAATGAACCCTGGCCGGATTTAATTCTCCTTGACT
GGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCGCGAGTCGATGACCCGGGATATTCCAGTGGTG
ATGTTGACCGCCAGAGGGGAAGAAGAAGATCGCGTGCGCGGCCTTGAAACCGGCGCGGATGACTATATCACCAAGCCATT
TTCGCCGAAGGAGCTGGTGGCGCGAATCAAAGCGGTAATGCGCCGTATTTCGCCAATGGCGGTGGAAGAGGTGATTAAGA
TGCAGGGATTAAGTCTCAACCCGACATCTCACCGAGTGATGGCGGGCGAAGAGCCGCTGGAGATGGGGCCGACAGAATTT
AAACTGCTGCACTTTTTTATGACGCATCCTGAGCGCGTGTACAGCCGCGAGCAGCTGTTAAACCACGTCTGGGGAACTAA
CGTGTATGTGGAAGACCGCACGGTCGATGTCCACATTCGTCGCCTGCGTAAAGCACTGGAGCCCGGCGGGCATGACCGCA
TGGTGCAGACCGTGCGCGGTACAGGATATCGTTTTTCAACCCGCTTTTAA

Upstream 100 bases:

>100_bases
GAAGATATGTGCGACGAGCTTTTCATAAATCTGTCATAAATCTGACGCATAATGACGTCGCATTAATGATCGCAACCTAT
TTATTACAACAGGGCAAATC

Downstream 100 bases:

>100_bases
CGCCTTGCTCATCGGACGCAGAGCAGGGCTTATGATTTCTTAACTGGAGTATCTTACGTGCTGGAACGGCTGTCGTGGAA
AAGGCTGGTGCTGGAGCTGC

Product: transcriptional regulator PhoB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNEPWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVV
MLTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTVRGTGYRFSTRF

Sequences:

>Translated_229_residues
MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNEPWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVV
MLTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTVRGTGYRFSTRF
>Mature_228_residues
ARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNEPWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVM
LTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEFK
LLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTVRGTGYRFSTRF

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=229, Percent_Identity=99.1266375545851, Blast_Score=467, Evalue=1e-133,
Organism=Escherichia coli, GI1788394, Length=224, Percent_Identity=39.7321428571429, Blast_Score=160, Evalue=5e-41,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=37.9912663755458, Blast_Score=150, Evalue=7e-38,
Organism=Escherichia coli, GI1786911, Length=223, Percent_Identity=36.7713004484305, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1790860, Length=221, Percent_Identity=37.10407239819, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=34.9775784753363, Blast_Score=133, Evalue=9e-33,
Organism=Escherichia coli, GI1786784, Length=220, Percent_Identity=33.6363636363636, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=39.3013100436681, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1787375, Length=225, Percent_Identity=32.4444444444444, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1790863, Length=231, Percent_Identity=33.3333333333333, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=33.1818181818182, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1787229, Length=230, Percent_Identity=32.6086956521739, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789402, Length=225, Percent_Identity=33.7777777777778, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI145693140, Length=236, Percent_Identity=27.5423728813559, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788191, Length=122, Percent_Identity=28.6885245901639, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788550, Length=109, Percent_Identity=36.697247706422, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26432; Mature: 26301

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNEPWPDLILLDWMLPGGS
CCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCC
GIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVM
HHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHCCCCCCCHHCCCCCCHHHHHHHHHHHH
RRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEFKLLHFFMTHPERVYSREQLL
HHCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHH
NHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTVRGTGYRFSTRF
HHHCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCC
>Mature Secondary Structure 
ARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNEPWPDLILLDWMLPGGS
CCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCC
GIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDYITKPFSPKELVARIKAVM
HHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHCCCCCCCHHCCCCCCHHHHHHHHHHHH
RRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEFKLLHFFMTHPERVYSREQLL
HHCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHH
NHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTVRGTGYRFSTRF
HHHCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]