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The map label for this gene is yajF

Identifier: 161486537

GI number: 161486537

Start: 338894

End: 339787

Strand: Direct

Name: yajF

Synonym: S0338

Alternate gene names: 161486537

Gene position: 338894-339787 (Clockwise)

Preceding gene: 30061889

Following gene: 30061895

Centisome position: 7.37

GC content: 55.7

Gene sequence:

>894_bases
GTGCGTATAGGTATCGATTTAGGCGGCACCAAAACTGAAGTGATTGCACTGGGCGATGCAGGGGAGCAGTTGTACCGCCA
TCGTCTGCCCACGCCGCGTGATGATTACCGGCAGACTATTGAAACGATCGCCACGTTGGTTGATATGGCGGAGCAGGCGA
CAGGGCAGCGCGGAACGGTAGGTATGGGCATTCCAGGTTCGATTTCGCCCTACACCGGTGTGGTGAAGAATGTCAATTCA
ACCTGGCTCAACGGTCAGCCATTCGATAAAGACTTAAGCGCGAGGTTGCAGCGGGAAGTGCGGCTGGCAAATGATGCCAA
CTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGAT
GCGGCGCGGGCGTGGCATTCAATGGGCGGACGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTA
CCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTT
TATTTCGGGCACGGGATTCGCGACGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGG
TTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTG
AATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTTATATCAAACGGTTGGGCAGTT
GATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGAGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCG
GCGCTGCGTGGTGA

Upstream 100 bases:

>100_bases
AAATTTAGCGGGCATGATAACGAATTGTCGGCGGCCTTGCATTGCCAATCCGGTTGTCCGTCTCTACGCTATTGATATTG
AAAAAAATAAGGAGAGTACA

Downstream 100 bases:

>100_bases
TGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCATCATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAA
ATGTGCCAGAACCGCGTAGG

Product: fructokinase

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIATLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNS
TWLNGQPFDKDLSARLQREVRLANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESDPVAELALRRYELRLAKSLAHVV
NILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGECETPVSKAKHGDSSGVRGAAW

Sequences:

>Translated_297_residues
MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIATLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNS
TWLNGQPFDKDLSARLQREVRLANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESDPVAELALRRYELRLAKSLAHVV
NILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGECETPVSKAKHGDSSGVRGAAW
>Mature_297_residues
MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIATLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNS
TWLNGQPFDKDLSARLQREVRLANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESDPVAELALRRYELRLAKSLAHVV
NILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGECETPVSKAKHGDSSGVRGAAW

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI87081733, Length=297, Percent_Identity=98.6531986531986, Blast_Score=601, Evalue=1e-173,
Organism=Escherichia coli, GI1787363, Length=305, Percent_Identity=40, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1786891, Length=272, Percent_Identity=22.7941176470588, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790522, Length=268, Percent_Identity=27.2388059701493, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 31805; Mature: 31805

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIATLVDMAEQATGQRGTV
CEEECCCCCCCEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRLANDANCLAVSEAVDGAAA
CCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHCCHHH
GAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPLPWMDEDELRYREEVPCYCGK
HHHHEEHHEEECCCCCCEEECCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCC
QGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESDPVAELALRRYELRLAKSLAHVV
HHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
NILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGECETPVSKAKHGDSSGVRGAAW
HHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIATLVDMAEQATGQRGTV
CEEECCCCCCCEEEEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRLANDANCLAVSEAVDGAAA
CCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHCCHHH
GAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPLPWMDEDELRYREEVPCYCGK
HHHHEEHHEEECCCCCCEEECCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCC
QGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESDPVAELALRRYELRLAKSLAHVV
HHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
NILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGECETPVSKAKHGDSSGVRGAAW
HHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]