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The map label for this gene is yafL

Identifier: 30061844

GI number: 30061844

Start: 296871

End: 297425

Strand: Direct

Name: yafL

Synonym: S0296

Alternate gene names: 30061844

Gene position: 296871-297425 (Clockwise)

Preceding gene: 30061842

Following gene: 30061845

Centisome position: 6.45

GC content: 54.95

Gene sequence:

>555_bases
ATGCGTTTTGCTCCCTTTACTCGCCAGCGCCACCACAACCGGGAGCAGATAAAACAGCATCCAGAATGGTTTCCTGCGCC
GCTCAAGGCGAGTGACAGACGCTGGCAGGCGCTGGCGGAAAACAACCACTTTTTAAGCAGCGATCACCTGCATAACATCA
CCGAAGTGGCGATTCACCGCCTGGAGCAGCAGCTTGGCAAGCCTTACGTCTGGGGCGGCACGCGGCCCGATCAAGGCTTC
GATTGTAGTGGGTTAGTTTTTTATGCCTACAACAAGATCCTTGAGGCGAAACTCCCGCGCACGGCCAATGAGATGTACCA
CTACCGTCGGGCAACGATTGTGGCGAATAACGACCTGCGCCGGGGAGATTTGCTATTTTTCCATATCCACAGCCGCGAGA
TTGCCGACCATATGGGGGGGTATCTGGGCGACGGTCAGTTTATTGAGTCACTGCGCACCGGGGAAACCATTCGGGTAAGC
CGGTTAGCCGAACCTTTCTGGCAGGACCATTTTTTAGGCGCGCGCAGGATTTTGACGGAAGAGATGATTTTGTAG

Upstream 100 bases:

>100_bases
CCTCCGGTCTTTTAAAATTCTCACCTTCATTCAGCCGGTTTATGTCTTTCATGTCTTCGTTCTTGCTCGGCAGATTTTTG
CATCCAGGCGTGTTTTCGTT

Downstream 100 bases:

>100_bases
GCCGGATAAGGCGTTTACGCCGCATCCGGCGGTTGTGCACGGATGCCTGATGCGACGCTGACGCGTCTTATCATGCCTAC
AAATCTGTGCGTTTTTGCGA

Product: putative lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MRFAPFTRQRHHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGF
DCSGLVFYAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGGYLGDGQFIESLRTGETIRVS
RLAEPFWQDHFLGARRILTEEMIL

Sequences:

>Translated_184_residues
MRFAPFTRQRHHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGF
DCSGLVFYAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGGYLGDGQFIESLRTGETIRVS
RLAEPFWQDHFLGARRILTEEMIL
>Mature_184_residues
MRFAPFTRQRHHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGF
DCSGLVFYAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGGYLGDGQFIESLRTGETIRVS
RLAEPFWQDHFLGARRILTEEMIL

Specific function: Unknown

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family [H]

Homologues:

Organism=Escherichia coli, GI1786421, Length=175, Percent_Identity=96.5714285714286, Blast_Score=358, Evalue=1e-101,
Organism=Escherichia coli, GI1787944, Length=134, Percent_Identity=50, Blast_Score=167, Evalue=4e-43,
Organism=Escherichia coli, GI1788501, Length=114, Percent_Identity=32.4561403508772, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 21676; Mature: 21676

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFAPFTRQRHHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHNITEVAIHR
CCCCCCHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHH
LEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAKLPRTANEMYHYRRATIVANNDLR
HHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEECCCCC
RGDLLFFHIHSREIADHMGGYLGDGQFIESLRTGETIRVSRLAEPFWQDHFLGARRILTE
CCCEEEEEECCHHHHHHHCCCCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHH
EMIL
HHCC
>Mature Secondary Structure
MRFAPFTRQRHHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHNITEVAIHR
CCCCCCHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHH
LEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAKLPRTANEMYHYRRATIVANNDLR
HHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEECCCCC
RGDLLFFHIHSREIADHMGGYLGDGQFIESLRTGETIRVSRLAEPFWQDHFLGARRILTE
CCCEEEEEECCHHHHHHHCCCCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHH
EMIL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]