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The map label for this gene is yafJ

Identifier: 30061842

GI number: 30061842

Start: 295042

End: 295809

Strand: Direct

Name: yafJ

Synonym: S0294

Alternate gene names: 30061842

Gene position: 295042-295809 (Clockwise)

Preceding gene: 30061841

Following gene: 30061844

Centisome position: 6.41

GC content: 53.65

Gene sequence:

>768_bases
ATGTGCGAATTGCTCGGGATGAGCGCCAACGTCCCTACCGATATCTGCTTTAGTTTCACCGGACTTGTACAGCGTGGTGG
TGGAACCGGGCCACATAAAGATGGCTGGGGCATTACCTTTTACGAAGGTAAAGGCTGTCGCACATTTAAAGATCCACAAC
CCAGCTTTAATTCCCCCATCGCCAAACTTGTCCAGGACTACCCGATAAAATCCTGTTCGGTGGTGGCTCATATTCGCCAG
GCTAATCGGGGCGAGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCA
TAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTCGGTGAAACCGACAGCGAAAAAGCCT
TTTGCTGGCTGCTGCATAAATTAACGCAGCGTTACCCGCGCACGCCGGGCAACATGGCGGCAGTGTTTAAATATATCGCC
TCACTGGCGGATGAACTGCGGGCGAAGGGCGTTTTCAACATGCTGCTTTCGGACGGGCGCTATGTAATGGCGTATTGCTC
GACTAATTTACACTGGATCACCCGCCGCGCGCCGTTTGGCGTGGCAACGTTGCTGGATCAGGATGTGGAAATCGACTTCA
GCTCGCAGACCACACCGAATGATGTGGTCACGGTGATTGCGACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAAATT
ATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGA

Upstream 100 bases:

>100_bases
ACCGTAGGCCGAATAATTCGTTTACGCCGCATCCGACCTGAAAATTCTTAAATCAATCTTCGCCGGGGTCCATGCGCTCC
CGCTGTTGTGGAGGTTACCC

Downstream 100 bases:

>100_bases
TGCCAGTTGTGGCTGCACCACTTCGTGGCTTAACGGCTTGCTGACCACGTAACGGCCATTGGCCACAGAAACGGTCGGTG
GCTTACGGGTTTGCTCAAAG

Product: putative amidotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGKGCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQ
ANRGEVALENTHPFTRELWGRNWTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTTPNDVVTVIATQPLTGNETWQKI
MPGEWRLFCLGERVV

Sequences:

>Translated_255_residues
MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGKGCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQ
ANRGEVALENTHPFTRELWGRNWTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTTPNDVVTVIATQPLTGNETWQKI
MPGEWRLFCLGERVV
>Mature_255_residues
MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGKGCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQ
ANRGEVALENTHPFTRELWGRNWTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTTPNDVVTVIATQPLTGNETWQKI
MPGEWRLFCLGERVV

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=255, Percent_Identity=99.6078431372549, Blast_Score=535, Evalue=1e-154,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 28579; Mature: 28579

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGKGCRTFKDPQPSFNSPI
CCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHH
AKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRNWTYAHNGQLTGYKSLETG
HHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHCCCCCCEEECCCCCCCCCCCCC
NFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIASLADELRAKGVFNMLLSDGR
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTTPNDVVTVIATQPLTGNETWQKI
EEEEEECCCEEEEEECCCCCHHHHHCCCCEEEECCCCCCCCEEEEEEECCCCCCHHHHHC
MPGEWRLFCLGERVV
CCCCEEEEEECCCCC
>Mature Secondary Structure
MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGKGCRTFKDPQPSFNSPI
CCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHH
AKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRNWTYAHNGQLTGYKSLETG
HHHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHCCCCCCEEECCCCCCCCCCCCC
NFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIASLADELRAKGVFNMLLSDGR
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTTPNDVVTVIATQPLTGNETWQKI
EEEEEECCCEEEEEECCCCCHHHHHCCCCEEEECCCCCCCCEEEEEEECCCCCCHHHHHC
MPGEWRLFCLGERVV
CCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]