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The map label for this gene is fruA

Identifier: 30024014

GI number: 30024014

Start: 985291

End: 987195

Strand: Direct

Name: fruA

Synonym: L185031

Alternate gene names: 30024014

Gene position: 985291-987195 (Clockwise)

Preceding gene: 15672940

Following gene: 15672942

Centisome position: 41.65

GC content: 40.1

Gene sequence:

>1905_bases
ATGGAAATAAAAGATTTACTTAAAACAGAGGTTATGATTCTTGACCTCCAAGCAACTAGCAAAGAAGCCGTTATTGATGA
AATGGTCAATAAATTAGTTTCTGAAGGTTATGTCAAAGATTTCGCAACTTTTAAAGCAGGAATTATGGCCCGTGAGGCAC
AGACTTCAACCGGACTTGGCGATGGAATTGCAATGCCTCACAGTAAAAATACAGCGGTCAATGAGCCAGTCGTTCTCTTT
GCAAAATCAAAAGTTGGTGTTGACTACGCCGCATTAGATGGACAACCTGTCAATCTTTTCTTCATGATAGCAGCACCAGA
CGGTGCTAATGATACTCACTTGGAAGCTTTAGCACAACTTTCAACTTATCTTCTTAAAGACGGTTTCCAAGATCGTTTGA
GAAAAGTTGAGTATCCAAGAGAGGTCATTCAGCTTTTTTCTGAAAAACAAGAAGAAATTAAGACTGTTGAAAATGCTGAA
CATTATATTGTAGCTGTAACGGCTTGTACAACGGGTATTGCTCATACTTATATGGCTGAAGAAGCGCTTAAAAAACAAGC
CTTGGAAATGGGCGTTGGAATTAAAGTTGAAACCAATGGTGCGCGTGGAATTGATAATAAATTAAGTGCAGCTGATATCC
AAAAAGCAGATGCTGTTATTGTGGCTGCGGATAAGAAAGTTGAAATGAATCGTTTTGCTGGTAAAGAAATGGTTCAAGTT
CCAGTTGCACATGCCATCCGAGATACTGAAGAATTAATTAATCGTGCACTGTCAGGTAAAGCGCCAATTTATGAAACAGA
CGGATCTGATGAAGTAAAAGAAGAAGCATCTGGTGGATTAGGAAAAGCATTCTATAAACACTTAATGGGTGGGGTTTCTG
CAATGCTTCCATTCGTTGTCGGTGGTGGGATACTTATTGCTCTTGCTTTCTTGATTGACCAAGCAATGGGAGTTCCTCAA
AGTCAATTGGCTAATCTAGGAGTTTATCATCCTTTAGCAGCTTACTTTAAAAATGTTGGTGGTGCAGCTTTTGCCTTTAT
GTTACCTGTTCTAGCTGGTTTCATTGCCAACTCTATTGCTGATAAACCTGGTTTAGTTGCTGGTTTTGTCGCTGGTTCTA
TGGCATCTTCTGGTCTTGCATTTGGTCATTTAGATCCTGCTAAAATGATTCCGTCTGGTTTCTTAGGAGCCCTAGTTGGT
GGTTTCCTTGCCGGTGGTGTCATTATCCTTTTACGTAAAGCTTTTGCAAAACTTCCTCGTTCACTTGATGGAATTAAAGC
TATCTTGATTTATCCATTAGTTGGAACATTTATTACAGGTTTCCTAATGCTCTTTGTCAATATCCCAATGGCAGCAATCA
ATACTGGTCTTTCTAACTTCTTAGCGAGTCTATCAGGAGCATCTGCTCTTGTACTTGGTTTAGTTGTTGGTGGAATGATG
GCTATTGACCTTGGGGGTCCAATTAACAAAGCTGCTTATATCTTTGCAACTGGTACTTTAGCAGCAACAGTTTCAACTGG
TGGTTCAGTAACAATGGCTGCCGTTATGGCTGGTGGGATGGTTCCACCACTCGCAACTACAGTAGCTGTTATCTTGTTTA
AAAATAAATTTACTAAAGAAGAACGCAATGCTGGTTTGACAAATATCGTTATGGGGCTTTCATTCATCACTGAAGGGTCA
ATTCCGTTTGGTGCATCTGACCCCGCACGCGCAATTCCATCATTTATGATTGGAGCAGCAGTAGCCGGTGGTTTGACTGG
TATGGCAAACATTAAATTGATGGCACCACATGGTGGAGTCTTCGTTCTCGCTTTGACAAACAATCCTTTACTTTATCTCT
TGTTCATCTTGATTGGCGCAATTGTTTCAGGCCTATTATTTGGTGCATTGAAAAAAGCAAAATAA

Upstream 100 bases:

>100_bases
CAATCGCTTGTGGAACTTCAAAGGCATTCTCAGATGATATGCCAAGCCGAGCATTTTTGGAAGAAATTTATAAAAAAGTT
AATATTTCAGAGGTGAAATG

Downstream 100 bases:

>100_bases
AAATGAATGAGAATAATAAGAGCGGTTTAATACCGCTTTTTTTGATATAATAGAGGAAGTAAACTGGGGGATTGGGAGAG
ATTATTTTGAGTTTTATTGC

Product: fructose-specific PTS system enzyme IIBC component

Products: NA

Alternate protein names: EIIABC-Fru; Fructose-specific phosphotransferase enzyme IIA component; EII-Fru; PTS system fructose-specific EIIA component; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 634; Mature: 634

Protein sequence:

>634_residues
MEIKDLLKTEVMILDLQATSKEAVIDEMVNKLVSEGYVKDFATFKAGIMAREAQTSTGLGDGIAMPHSKNTAVNEPVVLF
AKSKVGVDYAALDGQPVNLFFMIAAPDGANDTHLEALAQLSTYLLKDGFQDRLRKVEYPREVIQLFSEKQEEIKTVENAE
HYIVAVTACTTGIAHTYMAEEALKKQALEMGVGIKVETNGARGIDNKLSAADIQKADAVIVAADKKVEMNRFAGKEMVQV
PVAHAIRDTEELINRALSGKAPIYETDGSDEVKEEASGGLGKAFYKHLMGGVSAMLPFVVGGGILIALAFLIDQAMGVPQ
SQLANLGVYHPLAAYFKNVGGAAFAFMLPVLAGFIANSIADKPGLVAGFVAGSMASSGLAFGHLDPAKMIPSGFLGALVG
GFLAGGVIILLRKAFAKLPRSLDGIKAILIYPLVGTFITGFLMLFVNIPMAAINTGLSNFLASLSGASALVLGLVVGGMM
AIDLGGPINKAAYIFATGTLAATVSTGGSVTMAAVMAGGMVPPLATTVAVILFKNKFTKEERNAGLTNIVMGLSFITEGS
IPFGASDPARAIPSFMIGAAVAGGLTGMANIKLMAPHGGVFVLALTNNPLLYLLFILIGAIVSGLLFGALKKAK

Sequences:

>Translated_634_residues
MEIKDLLKTEVMILDLQATSKEAVIDEMVNKLVSEGYVKDFATFKAGIMAREAQTSTGLGDGIAMPHSKNTAVNEPVVLF
AKSKVGVDYAALDGQPVNLFFMIAAPDGANDTHLEALAQLSTYLLKDGFQDRLRKVEYPREVIQLFSEKQEEIKTVENAE
HYIVAVTACTTGIAHTYMAEEALKKQALEMGVGIKVETNGARGIDNKLSAADIQKADAVIVAADKKVEMNRFAGKEMVQV
PVAHAIRDTEELINRALSGKAPIYETDGSDEVKEEASGGLGKAFYKHLMGGVSAMLPFVVGGGILIALAFLIDQAMGVPQ
SQLANLGVYHPLAAYFKNVGGAAFAFMLPVLAGFIANSIADKPGLVAGFVAGSMASSGLAFGHLDPAKMIPSGFLGALVG
GFLAGGVIILLRKAFAKLPRSLDGIKAILIYPLVGTFITGFLMLFVNIPMAAINTGLSNFLASLSGASALVLGLVVGGMM
AIDLGGPINKAAYIFATGTLAATVSTGGSVTMAAVMAGGMVPPLATTVAVILFKNKFTKEERNAGLTNIVMGLSFITEGS
IPFGASDPARAIPSFMIGAAVAGGLTGMANIKLMAPHGGVFVLALTNNPLLYLLFILIGAIVSGLLFGALKKAK
>Mature_634_residues
MEIKDLLKTEVMILDLQATSKEAVIDEMVNKLVSEGYVKDFATFKAGIMAREAQTSTGLGDGIAMPHSKNTAVNEPVVLF
AKSKVGVDYAALDGQPVNLFFMIAAPDGANDTHLEALAQLSTYLLKDGFQDRLRKVEYPREVIQLFSEKQEEIKTVENAE
HYIVAVTACTTGIAHTYMAEEALKKQALEMGVGIKVETNGARGIDNKLSAADIQKADAVIVAADKKVEMNRFAGKEMVQV
PVAHAIRDTEELINRALSGKAPIYETDGSDEVKEEASGGLGKAFYKHLMGGVSAMLPFVVGGGILIALAFLIDQAMGVPQ
SQLANLGVYHPLAAYFKNVGGAAFAFMLPVLAGFIANSIADKPGLVAGFVAGSMASSGLAFGHLDPAKMIPSGFLGALVG
GFLAGGVIILLRKAFAKLPRSLDGIKAILIYPLVGTFITGFLMLFVNIPMAAINTGLSNFLASLSGASALVLGLVVGGMM
AIDLGGPINKAAYIFATGTLAATVSTGGSVTMAAVMAGGMVPPLATTVAVILFKNKFTKEERNAGLTNIVMGLSFITEGS
IPFGASDPARAIPSFMIGAAVAGGLTGMANIKLMAPHGGVFVLALTNNPLLYLLFILIGAIVSGLLFGALKKAK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=482, Percent_Identity=42.1161825726141, Blast_Score=338, Evalue=7e-94,
Organism=Escherichia coli, GI1786951, Length=616, Percent_Identity=35.8766233766234, Blast_Score=318, Evalue=5e-88,
Organism=Escherichia coli, GI87082348, Length=449, Percent_Identity=34.2984409799555, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1790386, Length=329, Percent_Identity=34.9544072948328, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1788729, Length=370, Percent_Identity=29.1891891891892, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1790387, Length=94, Percent_Identity=43.6170212765958, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI2367327, Length=129, Percent_Identity=26.3565891472868, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790390, Length=94, Percent_Identity=38.2978723404255, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788730, Length=87, Percent_Identity=39.0804597701149, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 66168; Mature: 66168

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00583 PFKB_KINASES_1 ; PS51094 PTS_EIIA_TYPE_2 ; PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIKDLLKTEVMILDLQATSKEAVIDEMVNKLVSEGYVKDFATFKAGIMAREAQTSTGLG
CCHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DGIAMPHSKNTAVNEPVVLFAKSKVGVDYAALDGQPVNLFFMIAAPDGANDTHLEALAQL
CCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHH
STYLLKDGFQDRLRKVEYPREVIQLFSEKQEEIKTVENAEHYIVAVTACTTGIAHTYMAE
HHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHH
EALKKQALEMGVGIKVETNGARGIDNKLSAADIQKADAVIVAADKKVEMNRFAGKEMVQV
HHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHCCEEEEECCCCCHHHHHCCHHHHHH
PVAHAIRDTEELINRALSGKAPIYETDGSDEVKEEASGGLGKAFYKHLMGGVSAMLPFVV
HHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
GGGILIALAFLIDQAMGVPQSQLANLGVYHPLAAYFKNVGGAAFAFMLPVLAGFIANSIA
CCHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
DKPGLVAGFVAGSMASSGLAFGHLDPAKMIPSGFLGALVGGFLAGGVIILLRKAFAKLPR
CCCCHHHHHHHHHHHHCCCEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SLDGIKAILIYPLVGTFITGFLMLFVNIPMAAINTGLSNFLASLSGASALVLGLVVGGMM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
AIDLGGPINKAAYIFATGTLAATVSTGGSVTMAAVMAGGMVPPLATTVAVILFKNKFTKE
EEECCCCCCCCEEEEEECCEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHH
ERNAGLTNIVMGLSFITEGSIPFGASDPARAIPSFMIGAAVAGGLTGMANIKLMAPHGGV
HHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCE
FVLALTNNPLLYLLFILIGAIVSGLLFGALKKAK
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEIKDLLKTEVMILDLQATSKEAVIDEMVNKLVSEGYVKDFATFKAGIMAREAQTSTGLG
CCHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
DGIAMPHSKNTAVNEPVVLFAKSKVGVDYAALDGQPVNLFFMIAAPDGANDTHLEALAQL
CCCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHH
STYLLKDGFQDRLRKVEYPREVIQLFSEKQEEIKTVENAEHYIVAVTACTTGIAHTYMAE
HHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHH
EALKKQALEMGVGIKVETNGARGIDNKLSAADIQKADAVIVAADKKVEMNRFAGKEMVQV
HHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHCCEEEEECCCCCHHHHHCCHHHHHH
PVAHAIRDTEELINRALSGKAPIYETDGSDEVKEEASGGLGKAFYKHLMGGVSAMLPFVV
HHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
GGGILIALAFLIDQAMGVPQSQLANLGVYHPLAAYFKNVGGAAFAFMLPVLAGFIANSIA
CCHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
DKPGLVAGFVAGSMASSGLAFGHLDPAKMIPSGFLGALVGGFLAGGVIILLRKAFAKLPR
CCCCHHHHHHHHHHHHCCCEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SLDGIKAILIYPLVGTFITGFLMLFVNIPMAAINTGLSNFLASLSGASALVLGLVVGGMM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
AIDLGGPINKAAYIFATGTLAATVSTGGSVTMAAVMAGGMVPPLATTVAVILFKNKFTKE
EEECCCCCCCCEEEEEECCEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHH
ERNAGLTNIVMGLSFITEGSIPFGASDPARAIPSFMIGAAVAGGLTGMANIKLMAPHGGV
HHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCE
FVLALTNNPLLYLLFILIGAIVSGLLFGALKKAK
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]