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The map label for this gene is clsA

Identifier: 15672942

GI number: 15672942

Start: 987282

End: 988727

Strand: Direct

Name: clsA

Synonym: L187016

Alternate gene names: 15672942

Gene position: 987282-988727 (Clockwise)

Preceding gene: 30024014

Following gene: 15672943

Centisome position: 41.74

GC content: 33.61

Gene sequence:

>1446_bases
TTGAGTTTTATTGCACTTTTTGAGCTGGCGCTTTTTTTCCTTAATGTTATATTATCCTTTGTCATTATTTTTAGGGAAAG
AAAATCAACAGCGGCAACTTGGTCATGGCTCTTTGTAGTTAATTTACTGCCTATCATAGGCTTTATTTTATATTTACTCA
TTGGACGTGGCATTTCGCATTATCGTATTTTTAAAGACCGAAAAAGGTTTCAGTTGGGTTTTGAAAAACAATTAGAACAT
GCTGAAAAATCTTACACAGATCGATTATTTATTAAAAAAATGAGACAAAATCATGTAATTGGTCAGTTGATTCATATGCT
TTATGTTGAAGAAAAATCAATTATTTCAACAAATACTGGTGTGAAAATCTTCAATGATGGACGTCAGAAATTTAATGCTC
TGATTGATGATATTAATAAAGCAAAACATCACGTTCATATGGAATATTATATTTTTCGGATGGATCGAATGGGTCATGAA
ATCTATGATGCTTTACTTGCAGCTGCAAAACGAGGAGTCGAAGTTAAACTTTTAATTGACGCTTGGGGTTCAAATAAAAC
AAAAATGTCTAATTTTAAAGAACTTCAGGAGGCTGGTGGGCATGTAGCGCAATTTTTTCCATTAATTATGCCTTTAGTTA
ATCCACGAACAAACTATCGTTTACACCGTAAAATTGTAGTTATTGATGGAATTATTGGATACACAGGTGGATTTAATGTT
GGTGATGAATATGCTTCAATTACAAAGAAATTTGGCTATTGGCGTGACAATCATTTACGTTTGACTGGTGATATTGTTTA
TAGTCTACAACATCGTTTTATTTTAGACTGGAATTCTCAACATCATTTTGAAGTGACCGAGGGAGAGCCTTATTTCCCAG
ATTCGATTGTTGAAGGTCATGTAGCGACTCAACTTGTAACTTCGGGACCAGATGAGGACAAAGAGCAAATTAAATTAACT
TACATGAAGATGATTTCAGGTGCAGAGCGAGAGATTATCATTCAAACCCCTTATTATATTCCGTCTGATGCTTTACATGA
ATCTATAAAATTAGCATTGTTATCGGGTGTACAGGTTAAATTATTGATTCCAAATAAGCCGGACCATCCACTGGTTTATT
GGGCGACTTATTTCCATGCTGCTGATTTAGTTAAATATGGCGCGAAAGTTTATACTTACGAAAATGGATTTGTTCACTCT
AAAACATTGATTATTGATGGAGAGTATGCCTCAGTTGGGTCAGCTAATTTAGATTATCGAAGTTTGCAATTATGTTTTGA
AGCTAATGTTGTTATCTATGATTATGACATTTCTCAAAAATTACGAAATGATTTTATGAAGGATTTGAAATTGAGTCGTC
CATTAACTTTAGAGCGTTATGAGGAACGCTCTAAGTTAGTTCGTTTTAAAGAAGGTTTAGCTCGTTTAATTGCACCAATG
CTCTGA

Upstream 100 bases:

>100_bases
AAAAAGCAAAATAAAAATGAATGAGAATAATAAGAGCGGTTTAATACCGCTTTTTTTGATATAATAGAGGAAGTAAACTG
GGGGATTGGGAGAGATTATT

Downstream 100 bases:

>100_bases
TACCTATTATTAAAAATTGAAGTAGTAAGATTTATTGAAGAAAAAATTTAAAAGAGTAAGATGAAAATGGAGGAGAGAAA
TGGAGAATAAACTCAATATT

Product: cardiolipin synthase

Products: NA

Alternate protein names: CL synthase 2 [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MSFIALFELALFFLNVILSFVIIFRERKSTAATWSWLFVVNLLPIIGFILYLLIGRGISHYRIFKDRKRFQLGFEKQLEH
AEKSYTDRLFIKKMRQNHVIGQLIHMLYVEEKSIISTNTGVKIFNDGRQKFNALIDDINKAKHHVHMEYYIFRMDRMGHE
IYDALLAAAKRGVEVKLLIDAWGSNKTKMSNFKELQEAGGHVAQFFPLIMPLVNPRTNYRLHRKIVVIDGIIGYTGGFNV
GDEYASITKKFGYWRDNHLRLTGDIVYSLQHRFILDWNSQHHFEVTEGEPYFPDSIVEGHVATQLVTSGPDEDKEQIKLT
YMKMISGAEREIIIQTPYYIPSDALHESIKLALLSGVQVKLLIPNKPDHPLVYWATYFHAADLVKYGAKVYTYENGFVHS
KTLIIDGEYASVGSANLDYRSLQLCFEANVVIYDYDISQKLRNDFMKDLKLSRPLTLERYEERSKLVRFKEGLARLIAPM
L

Sequences:

>Translated_481_residues
MSFIALFELALFFLNVILSFVIIFRERKSTAATWSWLFVVNLLPIIGFILYLLIGRGISHYRIFKDRKRFQLGFEKQLEH
AEKSYTDRLFIKKMRQNHVIGQLIHMLYVEEKSIISTNTGVKIFNDGRQKFNALIDDINKAKHHVHMEYYIFRMDRMGHE
IYDALLAAAKRGVEVKLLIDAWGSNKTKMSNFKELQEAGGHVAQFFPLIMPLVNPRTNYRLHRKIVVIDGIIGYTGGFNV
GDEYASITKKFGYWRDNHLRLTGDIVYSLQHRFILDWNSQHHFEVTEGEPYFPDSIVEGHVATQLVTSGPDEDKEQIKLT
YMKMISGAEREIIIQTPYYIPSDALHESIKLALLSGVQVKLLIPNKPDHPLVYWATYFHAADLVKYGAKVYTYENGFVHS
KTLIIDGEYASVGSANLDYRSLQLCFEANVVIYDYDISQKLRNDFMKDLKLSRPLTLERYEERSKLVRFKEGLARLIAPM
L
>Mature_480_residues
SFIALFELALFFLNVILSFVIIFRERKSTAATWSWLFVVNLLPIIGFILYLLIGRGISHYRIFKDRKRFQLGFEKQLEHA
EKSYTDRLFIKKMRQNHVIGQLIHMLYVEEKSIISTNTGVKIFNDGRQKFNALIDDINKAKHHVHMEYYIFRMDRMGHEI
YDALLAAAKRGVEVKLLIDAWGSNKTKMSNFKELQEAGGHVAQFFPLIMPLVNPRTNYRLHRKIVVIDGIIGYTGGFNVG
DEYASITKKFGYWRDNHLRLTGDIVYSLQHRFILDWNSQHHFEVTEGEPYFPDSIVEGHVATQLVTSGPDEDKEQIKLTY
MKMISGAEREIIIQTPYYIPSDALHESIKLALLSGVQVKLLIPNKPDHPLVYWATYFHAADLVKYGAKVYTYENGFVHSK
TLIIDGEYASVGSANLDYRSLQLCFEANVVIYDYDISQKLRNDFMKDLKLSRPLTLERYEERSKLVRFKEGLARLIAPML

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=493, Percent_Identity=27.789046653144, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1787007, Length=337, Percent_Identity=32.9376854599407, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI87081826, Length=381, Percent_Identity=24.9343832020997, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR015679
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 55827; Mature: 55695

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFIALFELALFFLNVILSFVIIFRERKSTAATWSWLFVVNLLPIIGFILYLLIGRGISH
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
YRIFKDRKRFQLGFEKQLEHAEKSYTDRLFIKKMRQNHVIGQLIHMLYVEEKSIISTNTG
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCC
VKIFNDGRQKFNALIDDINKAKHHVHMEYYIFRMDRMGHEIYDALLAAAKRGVEVKLLID
EEEECCHHHHHHHHHHHHHHHHHEEEEEHEEEEHHHHHHHHHHHHHHHHHCCCEEEEEEE
AWGSNKTKMSNFKELQEAGGHVAQFFPLIMPLVNPRTNYRLHRKIVVIDGIIGYTGGFNV
ECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEEECEEECCCCCCC
GDEYASITKKFGYWRDNHLRLTGDIVYSLQHRFILDWNSQHHFEVTEGEPYFPDSIVEGH
CHHHHHHHHHHCCCCCCCEEEEHHHHEEECCEEEEECCCCCCEEECCCCCCCCHHHHCCH
VATQLVTSGPDEDKEQIKLTYMKMISGAEREIIIQTPYYIPSDALHESIKLALLSGVQVK
HHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEE
LLIPNKPDHPLVYWATYFHAADLVKYGAKVYTYENGFVHSKTLIIDGEYASVGSANLDYR
EEECCCCCCCEEEEHHHHHHHHHHHCCCEEEEECCCEEEEEEEEEECCCCCCCCCCCCHH
SLQLCFEANVVIYDYDISQKLRNDFMKDLKLSRPLTLERYEERSKLVRFKEGLARLIAPM
HEEEEEECCEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
L
C
>Mature Secondary Structure 
SFIALFELALFFLNVILSFVIIFRERKSTAATWSWLFVVNLLPIIGFILYLLIGRGISH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
YRIFKDRKRFQLGFEKQLEHAEKSYTDRLFIKKMRQNHVIGQLIHMLYVEEKSIISTNTG
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCC
VKIFNDGRQKFNALIDDINKAKHHVHMEYYIFRMDRMGHEIYDALLAAAKRGVEVKLLID
EEEECCHHHHHHHHHHHHHHHHHEEEEEHEEEEHHHHHHHHHHHHHHHHHCCCEEEEEEE
AWGSNKTKMSNFKELQEAGGHVAQFFPLIMPLVNPRTNYRLHRKIVVIDGIIGYTGGFNV
ECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEEECEEECCCCCCC
GDEYASITKKFGYWRDNHLRLTGDIVYSLQHRFILDWNSQHHFEVTEGEPYFPDSIVEGH
CHHHHHHHHHHCCCCCCCEEEEHHHHEEECCEEEEECCCCCCEEECCCCCCCCHHHHCCH
VATQLVTSGPDEDKEQIKLTYMKMISGAEREIIIQTPYYIPSDALHESIKLALLSGVQVK
HHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEE
LLIPNKPDHPLVYWATYFHAADLVKYGAKVYTYENGFVHSKTLIIDGEYASVGSANLDYR
EEECCCCCCCEEEEHHHHHHHHHHHCCCEEEEECCCEEEEEEEEEECCCCCCCCCCCCHH
SLQLCFEANVVIYDYDISQKLRNDFMKDLKLSRPLTLERYEERSKLVRFKEGLARLIAPM
HEEEEEECCEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9150240; 9384377 [H]