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The map label for this gene is gatB

Identifier: 30023976

GI number: 30023976

Start: 167741

End: 169174

Strand: Direct

Name: gatB

Synonym: L0474

Alternate gene names: 30023976

Gene position: 167741-169174 (Clockwise)

Preceding gene: 15672146

Following gene: 15672148

Centisome position: 7.09

GC content: 38.77

Gene sequence:

>1434_bases
ATGAACTTTGAAACAGTAATTGGGCTTGAAGTCCACGTTGAGTTAAGTACAAACTCAAAAATTTTCAGTCCCGCCTCAAC
AAAATTTGGTGGAGACCCTAATACAAATACAAATGTGATTGACTGGTCACTTCCTGGTGTTTTACCAGTAATGAATAAAG
GGGTAATTGATAGCGGAATTAAAGCGGCTCTTGCTTTGAATATGGATATTCACAAATCAATGCATTTTGACCGCAAAAAT
TATTTCTATCCTGATAATCCCAAAGCTTACCAAATTTCACAATTTGATGAGCCAATTGGTTACAATGGTTCCATTGAAAT
TGAACTTGAAGATGGCCATAAAGCGACGATTCGCATTGAGCGCGCTCATTTAGAAGAAGATGCTGGGAAAAATACGCACG
GAACGGATGGTTATTCTTATGTCGATTTGAATCGTCAAGGAGTACCATTGATTGAGATTGTCTCAGAAGCTGACATGCGC
ACTCCTGAGGAAGCTTATGCTTATCTGACAGCACTTAAAGAAGCCATTCTTTACACAGGAATTTCTGATGTGAAGATGGA
AGAAGGTTCAATGCGTTGTGATGCCAATGTTTCGCTCCGTCCTTATGGTCAAGAAGCTTTTGGTGTGAAGACAGAAGTTA
AAAACATGAACTCATTTAGCAATGTCAAAAAAGCTTTGGACTTTGAAGTCGACAGGCAAGCTAAGATTTTGCGCGCTGGT
GGCGAAATTCGTCAAGAAACGCGTCGTTTTAATGACAAAACGGGTGAAACAATTTTGATGCGTGTTAAAGAAGGCGCTTC
TGATTACCGTTACTTCCCAGAGCCAGATGTCCCTCGTTTTGAAATTTCTGAGGAATGGGTTGAGCAAATGCGTGAGAGCC
TTCCAATGACGGCTAAAGCAAGACGTGCACATTATATTAATGATTTAGCTTTGTCTGATTATGATGCTCGTCAATTGACC
GCAACTAAAGAAGTTTCTGATTTCTTTGATCAAGCGATTAAATTTGATACTGACCCTAAATTGGTGTCAAACTGGTTGCA
AGGTGAAGTGGCTCAATATTTGAATAGTGAGAAAAAAGAACTTCATGAGATTGGCTTGACTCCAGAAAATCTGACAGAAA
TGATTCGTTTGATTTCAGATGGAACAATTTCTTCAAAAATTGCTAAAAAAGTCTTTATTGAATTAGCAAAAAATGGTGGT
TCTGCCGAAGAATTTGTCAAGAAAGCTGGTTTGGTTCAAATTTCTGACCCTGCTGTGCTTTTGCCAATCATTCATGATGT
ATTTGCTAAGAATGAACAATCTGTAGCAGATTATCGTGGTGGGAAGCAAAATGCGGCGAAAGCTTTGGTTGGTCAATTAA
TGAAAGCAACTAAAGGACAAGCTAACCCAACGGTTGCGCAAAAACTTTTGTATCAAGAATTGGATAATTTCTAA

Upstream 100 bases:

>100_bases
CATGTCACAACTTAGTTTGCTTGAAAAATTGTCAAAAACCGGAAAACAATATATTGACTAGGATAATTTGACTTTCTTAA
AGAAAGTATCGGAGAAAATA

Downstream 100 bases:

>100_bases
GGATCAGCTGTATGAAACAGCTATCTTACATTTCATTCGGTCATAAGTTAGTGGAAGACAGCAATATGAAAACTTTATTT
TGTTTTGCCAGTTATAATAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MNFETVIGLEVHVELSTNSKIFSPASTKFGGDPNTNTNVIDWSLPGVLPVMNKGVIDSGIKAALALNMDIHKSMHFDRKN
YFYPDNPKAYQISQFDEPIGYNGSIEIELEDGHKATIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
TPEEAYAYLTALKEAILYTGISDVKMEEGSMRCDANVSLRPYGQEAFGVKTEVKNMNSFSNVKKALDFEVDRQAKILRAG
GEIRQETRRFNDKTGETILMRVKEGASDYRYFPEPDVPRFEISEEWVEQMRESLPMTAKARRAHYINDLALSDYDARQLT
ATKEVSDFFDQAIKFDTDPKLVSNWLQGEVAQYLNSEKKELHEIGLTPENLTEMIRLISDGTISSKIAKKVFIELAKNGG
SAEEFVKKAGLVQISDPAVLLPIIHDVFAKNEQSVADYRGGKQNAAKALVGQLMKATKGQANPTVAQKLLYQELDNF

Sequences:

>Translated_477_residues
MNFETVIGLEVHVELSTNSKIFSPASTKFGGDPNTNTNVIDWSLPGVLPVMNKGVIDSGIKAALALNMDIHKSMHFDRKN
YFYPDNPKAYQISQFDEPIGYNGSIEIELEDGHKATIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
TPEEAYAYLTALKEAILYTGISDVKMEEGSMRCDANVSLRPYGQEAFGVKTEVKNMNSFSNVKKALDFEVDRQAKILRAG
GEIRQETRRFNDKTGETILMRVKEGASDYRYFPEPDVPRFEISEEWVEQMRESLPMTAKARRAHYINDLALSDYDARQLT
ATKEVSDFFDQAIKFDTDPKLVSNWLQGEVAQYLNSEKKELHEIGLTPENLTEMIRLISDGTISSKIAKKVFIELAKNGG
SAEEFVKKAGLVQISDPAVLLPIIHDVFAKNEQSVADYRGGKQNAAKALVGQLMKATKGQANPTVAQKLLYQELDNF
>Mature_477_residues
MNFETVIGLEVHVELSTNSKIFSPASTKFGGDPNTNTNVIDWSLPGVLPVMNKGVIDSGIKAALALNMDIHKSMHFDRKN
YFYPDNPKAYQISQFDEPIGYNGSIEIELEDGHKATIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
TPEEAYAYLTALKEAILYTGISDVKMEEGSMRCDANVSLRPYGQEAFGVKTEVKNMNSFSNVKKALDFEVDRQAKILRAG
GEIRQETRRFNDKTGETILMRVKEGASDYRYFPEPDVPRFEISEEWVEQMRESLPMTAKARRAHYINDLALSDYDARQLT
ATKEVSDFFDQAIKFDTDPKLVSNWLQGEVAQYLNSEKKELHEIGLTPENLTEMIRLISDGTISSKIAKKVFIELAKNGG
SAEEFVKKAGLVQISDPAVLLPIIHDVFAKNEQSVADYRGGKQNAAKALVGQLMKATKGQANPTVAQKLLYQELDNF

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=495, Percent_Identity=36.1616161616162, Blast_Score=295, Evalue=9e-80,
Organism=Caenorhabditis elegans, GI115534094, Length=343, Percent_Identity=34.6938775510204, Blast_Score=211, Evalue=6e-55,
Organism=Saccharomyces cerevisiae, GI6319391, Length=502, Percent_Identity=28.2868525896414, Blast_Score=184, Evalue=2e-47,
Organism=Drosophila melanogaster, GI45550808, Length=496, Percent_Identity=31.0483870967742, Blast_Score=240, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_LACLA (Q9CJ41)

Other databases:

- EMBL:   AE005176
- PIR:   E86645
- RefSeq:   NP_266321.2
- ProteinModelPortal:   Q9CJ41
- SMR:   Q9CJ41
- GeneID:   1113773
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0474
- NMPDR:   fig|272623.1.peg.172
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   LLAC272623:L0474-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53426; Mature: 53426

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFETVIGLEVHVELSTNSKIFSPASTKFGGDPNTNTNVIDWSLPGVLPVMNKGVIDSGI
CCCCEEEEEEEEEEECCCCEEECCCCCCCCCCCCCCCEEEEEECCCHHHHHCCCCHHCCC
KAALALNMDIHKSMHFDRKNYFYPDNPKAYQISQFDEPIGYNGSIEIELEDGHKATIRIE
CEEEEECCHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEEECCCEEEEEEE
RAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMRTPEEAYAYLTALKEAILYTG
HHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
ISDVKMEEGSMRCDANVSLRPYGQEAFGVKTEVKNMNSFSNVKKALDFEVDRQAKILRAG
CCCCEECCCCCEECCCCEECCCCCHHHCCHHHHHCCHHHHHHHHHHCCCCCHHHHHHHCC
GEIRQETRRFNDKTGETILMRVKEGASDYRYFPEPDVPRFEISEEWVEQMRESLPMTAKA
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCEECCHHHHHHHHHHCCCCHHH
RRAHYINDLALSDYDARQLTATKEVSDFFDQAIKFDTDPKLVSNWLQGEVAQYLNSEKKE
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHH
LHEIGLTPENLTEMIRLISDGTISSKIAKKVFIELAKNGGSAEEFVKKAGLVQISDPAVL
HHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCEEH
LPIIHDVFAKNEQSVADYRGGKQNAAKALVGQLMKATKGQANPTVAQKLLYQELDNF
HHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNFETVIGLEVHVELSTNSKIFSPASTKFGGDPNTNTNVIDWSLPGVLPVMNKGVIDSGI
CCCCEEEEEEEEEEECCCCEEECCCCCCCCCCCCCCCEEEEEECCCHHHHHCCCCHHCCC
KAALALNMDIHKSMHFDRKNYFYPDNPKAYQISQFDEPIGYNGSIEIELEDGHKATIRIE
CEEEEECCHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEEEECCCEEEEEEE
RAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMRTPEEAYAYLTALKEAILYTG
HHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
ISDVKMEEGSMRCDANVSLRPYGQEAFGVKTEVKNMNSFSNVKKALDFEVDRQAKILRAG
CCCCEECCCCCEECCCCEECCCCCHHHCCHHHHHCCHHHHHHHHHHCCCCCHHHHHHHCC
GEIRQETRRFNDKTGETILMRVKEGASDYRYFPEPDVPRFEISEEWVEQMRESLPMTAKA
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCEECCHHHHHHHHHHCCCCHHH
RRAHYINDLALSDYDARQLTATKEVSDFFDQAIKFDTDPKLVSNWLQGEVAQYLNSEKKE
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHH
LHEIGLTPENLTEMIRLISDGTISSKIAKKVFIELAKNGGSAEEFVKKAGLVQISDPAVL
HHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCEEH
LPIIHDVFAKNEQSVADYRGGKQNAAKALVGQLMKATKGQANPTVAQKLLYQELDNF
HHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471