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The map label for this gene is ybgE

Identifier: 15672148

GI number: 15672148

Start: 169398

End: 170897

Strand: Direct

Name: ybgE

Synonym: L169390

Alternate gene names: 15672148

Gene position: 169398-170897 (Clockwise)

Preceding gene: 30023976

Following gene: 15672151

Centisome position: 7.16

GC content: 40.2

Gene sequence:

>1500_bases
ATGCTTGAAATTAAAGATGCAACTTATTGGGCGGAGCAATTAAAATCAGGAAATCTTTCTGCCAAAGCGTTATTAGAAAT
GACAAATGACAAGATTCAAAAGCTCAATCCGCGTTACAATGCGATTGTTGCTTACGATTTAGAAAAGGCAAAAGCTGATT
TAGCTTTAACGAGTCAAGGTTTTTTTGCTGGTCTTCCTTTTGCTTTGAAAATGTTGGGACAAACTCATAAAGGATTGCCT
GATACGGCTAGCTCTCACCTCTTTTCAGAGAATGTGGCATCAGAGGATGACAACTATGTTAAAGGACTCTTAGAAGCTGG
TTTCACGCCATTTGGACAAACGAATTCTCCAGAGTTTGGCTTTAAAAACATTTCTGATTCAAAACTCTACGGAGATACAC
GAAATGTCTGGAATGAAGCTTATTATTCGGGAGGGTCTTCTGGCGGAGCGGCGTCTGCCGTGGTTTCTGGGATGTTTGCA
ATTGCTGGAGCTTCAGATGGCGGTGGGTCGATTCGGATTCCAGCCTCATTTTCGGGCTTGATTGGACTTAAAATGACGAG
AGGGCGGATGCCACAAGGGCCTGATTCTTACCGAGGATGGCAAGGAGCATCGATTTCTGGAGCTCTTACTGTATCTGTGC
GAGATACGGCTCGTTTTGTGCTTGAAATGCAAACCCTACAAGAAGCAGCACCTTATCAGGCAAAATTGTTGGACGAAAAA
GAACTTATAGCGCTCACTAAAAGTACTGGCCAAGCTGTCAGCAATAATTTATCAAGAAAAATAAAAATTGCTTATTCACT
CCAAAGCCCGATTTCTGGAATTGAATTATCAAAAGACGCCATAAATGCATGCCTAAAGGCAGTAGAGTTTTTAGAGCGTC
AAGGTCATGAATTGACAGAAGTTGATTTTCCCATAGAAGCTCGTCCTTTGATTCAGTCTTATTATCGTATGAATGCGGCT
GAAATAGTTGCCATGTTAGAACCTTGGGAACGGGCTGCCGGACGAAAATTGACGAGTCAAGATGTTGAACTTTTGACTTA
TGCGCTTTTAGAAGCCGGAAGAAAAGCTCCGGTGTCTTCTTATATCAACGCTTTAGCTCAGTGGGATCTTGCGGCTAGAG
ATTTTACTGACAAAATTTATAAAGATTATGATTTATTTTTGACGGCAACAACTGCTAAAACAGCCCCTAAAATTGGTCAG
GAATTAATGACAGCGGAAGTATTAAAAGAAATGTCAGAAATTAGCCAATATGAATTTGAACAACAAATAGAGATTATTGA
AGCCGCTTTTGAGCGTTCATTAGCTTTTACACCTTATAACTTCATCAGTAATTTAACGGGTCAGCCTGCGCTTTCTCTGC
CCGTTTATGTTAATGAAGAAACGAATCTGCCACTTGGAATTCAACTTTGGGGACCAAAAAATTCTGAAATTGAATTATTG
AAAGTTGCTAAAGAATTTGAGGAAGCGGGTCAATTTATTTTACCCAAAGCTTATCAATAA

Upstream 100 bases:

>100_bases
AACGGATATTTTTTATTATGATTGATAAGTATGGTAAAATGTTGGCAGAATGAGTTTGTCAGCAGATATATATATAAAAC
TCAGCTTTTAAAGGAGAACC

Downstream 100 bases:

>100_bases
GTTTAACGATATTTTACTGACAGAAAGTGTAAACCAAATCTGTCAGTAAATTTAAAAGAAAAACCGCCCTAAGTGGCGGT
TTTATGCTAAATCCTGATTT

Product: amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MLEIKDATYWAEQLKSGNLSAKALLEMTNDKIQKLNPRYNAIVAYDLEKAKADLALTSQGFFAGLPFALKMLGQTHKGLP
DTASSHLFSENVASEDDNYVKGLLEAGFTPFGQTNSPEFGFKNISDSKLYGDTRNVWNEAYYSGGSSGGAASAVVSGMFA
IAGASDGGGSIRIPASFSGLIGLKMTRGRMPQGPDSYRGWQGASISGALTVSVRDTARFVLEMQTLQEAAPYQAKLLDEK
ELIALTKSTGQAVSNNLSRKIKIAYSLQSPISGIELSKDAINACLKAVEFLERQGHELTEVDFPIEARPLIQSYYRMNAA
EIVAMLEPWERAAGRKLTSQDVELLTYALLEAGRKAPVSSYINALAQWDLAARDFTDKIYKDYDLFLTATTAKTAPKIGQ
ELMTAEVLKEMSEISQYEFEQQIEIIEAAFERSLAFTPYNFISNLTGQPALSLPVYVNEETNLPLGIQLWGPKNSEIELL
KVAKEFEEAGQFILPKAYQ

Sequences:

>Translated_499_residues
MLEIKDATYWAEQLKSGNLSAKALLEMTNDKIQKLNPRYNAIVAYDLEKAKADLALTSQGFFAGLPFALKMLGQTHKGLP
DTASSHLFSENVASEDDNYVKGLLEAGFTPFGQTNSPEFGFKNISDSKLYGDTRNVWNEAYYSGGSSGGAASAVVSGMFA
IAGASDGGGSIRIPASFSGLIGLKMTRGRMPQGPDSYRGWQGASISGALTVSVRDTARFVLEMQTLQEAAPYQAKLLDEK
ELIALTKSTGQAVSNNLSRKIKIAYSLQSPISGIELSKDAINACLKAVEFLERQGHELTEVDFPIEARPLIQSYYRMNAA
EIVAMLEPWERAAGRKLTSQDVELLTYALLEAGRKAPVSSYINALAQWDLAARDFTDKIYKDYDLFLTATTAKTAPKIGQ
ELMTAEVLKEMSEISQYEFEQQIEIIEAAFERSLAFTPYNFISNLTGQPALSLPVYVNEETNLPLGIQLWGPKNSEIELL
KVAKEFEEAGQFILPKAYQ
>Mature_499_residues
MLEIKDATYWAEQLKSGNLSAKALLEMTNDKIQKLNPRYNAIVAYDLEKAKADLALTSQGFFAGLPFALKMLGQTHKGLP
DTASSHLFSENVASEDDNYVKGLLEAGFTPFGQTNSPEFGFKNISDSKLYGDTRNVWNEAYYSGGSSGGAASAVVSGMFA
IAGASDGGGSIRIPASFSGLIGLKMTRGRMPQGPDSYRGWQGASISGALTVSVRDTARFVLEMQTLQEAAPYQAKLLDEK
ELIALTKSTGQAVSNNLSRKIKIAYSLQSPISGIELSKDAINACLKAVEFLERQGHELTEVDFPIEARPLIQSYYRMNAA
EIVAMLEPWERAAGRKLTSQDVELLTYALLEAGRKAPVSSYINALAQWDLAARDFTDKIYKDYDLFLTATTAKTAPKIGQ
ELMTAEVLKEMSEISQYEFEQQIEIIEAAFERSLAFTPYNFISNLTGQPALSLPVYVNEETNLPLGIQLWGPKNSEIELL
KVAKEFEEAGQFILPKAYQ

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=177, Percent_Identity=31.0734463276836, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI166795287, Length=182, Percent_Identity=34.6153846153846, Blast_Score=70, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17556264, Length=245, Percent_Identity=30.2040816326531, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17538252, Length=212, Percent_Identity=26.8867924528302, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71990152, Length=150, Percent_Identity=34, Blast_Score=69, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17537465, Length=209, Percent_Identity=29.1866028708134, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24644968, Length=201, Percent_Identity=28.8557213930348, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24652985, Length=265, Percent_Identity=29.0566037735849, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19922090, Length=265, Percent_Identity=29.0566037735849, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24652981, Length=265, Percent_Identity=29.0566037735849, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24652983, Length=265, Percent_Identity=29.0566037735849, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 54732; Mature: 54732

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEIKDATYWAEQLKSGNLSAKALLEMTNDKIQKLNPRYNAIVAYDLEKAKADLALTSQG
CCEECHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHCCEEEECCC
FFAGLPFALKMLGQTHKGLPDTASSHLFSENVASEDDNYVKGLLEAGFTPFGQTNSPEFG
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
FKNISDSKLYGDTRNVWNEAYYSGGSSGGAASAVVSGMFAIAGASDGGGSIRIPASFSGL
CCCCCCCHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEECCCCCCEEEECCCCCCC
IGLKMTRGRMPQGPDSYRGWQGASISGALTVSVRDTARFVLEMQTLQEAAPYQAKLLDEK
EEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCHHHHCCCH
ELIALTKSTGQAVSNNLSRKIKIAYSLQSPISGIELSKDAINACLKAVEFLERQGHELTE
HHEEEECCHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
VDFPIEARPLIQSYYRMNAAEIVAMLEPWERAAGRKLTSQDVELLTYALLEAGRKAPVSS
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHH
YINALAQWDLAARDFTDKIYKDYDLFLTATTAKTAPKIGQELMTAEVLKEMSEISQYEFE
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QQIEIIEAAFERSLAFTPYNFISNLTGQPALSLPVYVNEETNLPLGIQLWGPKNSEIELL
HHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEECCEEECCCCCCCEEEEEECCCCCCHHHH
KVAKEFEEAGQFILPKAYQ
HHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLEIKDATYWAEQLKSGNLSAKALLEMTNDKIQKLNPRYNAIVAYDLEKAKADLALTSQG
CCEECHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHCCEEEECCC
FFAGLPFALKMLGQTHKGLPDTASSHLFSENVASEDDNYVKGLLEAGFTPFGQTNSPEFG
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
FKNISDSKLYGDTRNVWNEAYYSGGSSGGAASAVVSGMFAIAGASDGGGSIRIPASFSGL
CCCCCCCHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEECCCCCCEEEECCCCCCC
IGLKMTRGRMPQGPDSYRGWQGASISGALTVSVRDTARFVLEMQTLQEAAPYQAKLLDEK
EEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCHHHHCCCH
ELIALTKSTGQAVSNNLSRKIKIAYSLQSPISGIELSKDAINACLKAVEFLERQGHELTE
HHEEEECCHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
VDFPIEARPLIQSYYRMNAAEIVAMLEPWERAAGRKLTSQDVELLTYALLEAGRKAPVSS
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHH
YINALAQWDLAARDFTDKIYKDYDLFLTATTAKTAPKIGQELMTAEVLKEMSEISQYEFE
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QQIEIIEAAFERSLAFTPYNFISNLTGQPALSLPVYVNEETNLPLGIQLWGPKNSEIELL
HHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEECCEEECCCCCCCEEEEEECCCCCCHHHH
KVAKEFEEAGQFILPKAYQ
HHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]