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The map label for this gene is pfl [H]

Identifier: 29350146

GI number: 29350146

Start: 6214685

End: 6216913

Strand: Reverse

Name: pfl [H]

Synonym: BT_4738

Alternate gene names: 29350146

Gene position: 6216913-6214685 (Counterclockwise)

Preceding gene: 29350148

Following gene: 29350145

Centisome position: 99.31

GC content: 50.61

Gene sequence:

>2229_bases
ATGGAATTAAACAAGATCTTTAAAGACGGTCTTTGGAGCAGCGAAATCAACGTCAGAGATTTCGTAAGTCATAACATCAC
TCCGTATTACGGAGATGCTTCATTCCTCGAAGGACCTACAGAACGTACCAAAGCCGTATGGAACCGCTGCCTCGAAGCGT
TGGCAGAAGAAAGAGAAAACAACGGTGTCCGCTCATTAGATAATGTTACCGTATCAACCATCACTTCCCACAAGGCCGGA
TATATCGACAAGGAAAACGAACTGATCGTCGGCCTGCAGACAGACGAACTTCTGAAACGTGCCATCAAGCCTTTCGGAGG
TATCAACGTAGTCAGCAAAGCCTGTCACGAAAACGGCGTGGAAGTGGACGACCGCGTAAAAGATATTTTCACTCACTACC
GCAAGACGCACAACGACGGAGTATTCGACGTATATACGGAAGAAATCCGTTCCTTCCGTTCGCTGGGATTCCTTACCGGA
CTTCCCGACAACTATGCACGCGGACGCATCATCGGTGACTACCGCCGTATGGCTCTTTACGGCATCGACCGTCTGATCGA
AGCAAAGAAGGAAGATTTGCACAACCTCACCGGTCCGATGACAGATGCCCGTATCCGTCTGCGCGAAGAAGTGGCAGAAC
AGATCAAGGCACTGAAAGACATGAAAGTGATGGGCGAATACTACGGTCTCGACCTGAGCCGTCCCGCTTACACGGCACAA
GAAGCCGTACAGTGGGTATATATGGCTTACCTTGCCGCCGTCAAAGAACAAGACGGTGCCGCCATGTCACTGGGTAACGT
TTCTTCTTTCCTCGATATCTATCTGGAATATGAACTGAGCAAAGGAACCATCACCGAATCGTTCGCGCAAGAGCTGATCG
ACCAGTTTGTCATCAAACTGCGTATGGTCCGCCACCTGCGTATGCAATCATACAACGACATCTTTGCAGGCGACCCGACC
TGGGTAACCGAATCTCTGGGCGGACGTCTCAACGACGGACGTACCAAAGTGACAAAGACCTCCTTCCGTTTCCTGCAGAC
ATTGTACAACCTCGGCCCTTCACCGGAACCGAACCTGACCGTACTCTGGAGCCCGGAACTTCCCGAAGGATTCAAAGAAT
TCTGTGCAAAGGTTTCTATCGACACTTCTTCTATCCAGTACGAAAACGACGACCTGATGCGTGAAGTACGTCAGTCCGAC
GACTACGGAATCGCCTGCTGCGTATCATACCAGGAAATAGGCAAGCAGATTCAGTTCTTCGGTGCCCGTTGCAACCTGGC
CAAAGCCCTGTTGCTTGCCATCAACGGCGGACGTTGCGAGAATACCGGTACAGTCATGGTGAAGAACATCCCCGTACTGA
CCAGTGACACGCTGAAGTTCGAAGAAGTAATGGACAACTACAAGAAAGTACTGATCGAAATCGCCCGCGTATACAACGAA
GCGATGAACATCATCCACTATATGCACGACAAGTATTATTACGAGAAAGCCCAGATGGCTCTTGTAGATACGAACCCGCG
CATAAACCTTGCTTACGGTGTAGCCGGACTTTCCATCGCACTCGATTCACTGTCTGCCATCAAATATGCGAAAGTAACTG
CCCGCCGCAACGATATCGGTCTGACAGAAGGCTTCGACATCGAAGGAGAATTCCCTTGCTTCGGTAACGACAACGACAAG
GTAGACCACCTTGGCGTAGACCTGGTATACTTCTTCAGCGAAGAATTGAAGAAATTGCCTGTTTACAAGAATGCCCGTCC
TACCCTCTCCTTGCTGACTATCACTTCCAATGTGATGTACGGCAAAAAGACCGGTGCTACTCCCGACGGACGTGCCAAAG
GTGTTGCCTTCGCTCCGGGTGCCAACCCGATGCACGGACGCGACAAGAATGGTGCGATCGCTTCTCTGAGCTCTGTAGCG
AAACTTCGTTACCGTGACTCACAGGACGGTATCAGCAACACCTTCTCTATCGTTCCGAAATCACTGGGTGCTACGGACGA
AGACCGTATCGAGAACCTCGTAACGATGATGGACGGTTACTTCACCAAAGGTGCCCACCACCTGAACGTAAACGTACTGA
ACCGTGATATGCTATACGATGCCATGGAGCATCCGGAGAACTATCCGCAGCTGACCATCCGCGTTTCCGGTTATGCCGTA
AACTTCGTGAAGTTGAGCCGCGAACACCAACTGGAAGTTATCAGCCGTAGCTTCCACGAACGTATGTAA

Upstream 100 bases:

>100_bases
GCATAAAATAGAGCATATTTATGCTGATTATATGAAAAATGATTGTAACTTTGTGCGCTCTAAAGAATAGGATAACTACA
ACCTCTAATTTTATCATTTT

Downstream 100 bases:

>100_bases
TCTATTACTCTATATGATGATAAACGTACATTCATACGAAAGTATGGGAACATTCGACGGGCCGGGCTTACGGCTCGTCG
TTTTTCTTCAAGGATGCAAC

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 742; Mature: 742

Protein sequence:

>742_residues
MELNKIFKDGLWSSEINVRDFVSHNITPYYGDASFLEGPTERTKAVWNRCLEALAEERENNGVRSLDNVTVSTITSHKAG
YIDKENELIVGLQTDELLKRAIKPFGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTG
LPDNYARGRIIGDYRRMALYGIDRLIEAKKEDLHNLTGPMTDARIRLREEVAEQIKALKDMKVMGEYYGLDLSRPAYTAQ
EAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSKGTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPT
WVTESLGGRLNDGRTKVTKTSFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQSD
DYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTLKFEEVMDNYKKVLIEIARVYNE
AMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGLSIALDSLSAIKYAKVTARRNDIGLTEGFDIEGEFPCFGNDNDK
VDHLGVDLVYFFSEELKKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVA
KLRYRDSQDGISNTFSIVPKSLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLNRDMLYDAMEHPENYPQLTIRVSGYAV
NFVKLSREHQLEVISRSFHERM

Sequences:

>Translated_742_residues
MELNKIFKDGLWSSEINVRDFVSHNITPYYGDASFLEGPTERTKAVWNRCLEALAEERENNGVRSLDNVTVSTITSHKAG
YIDKENELIVGLQTDELLKRAIKPFGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTG
LPDNYARGRIIGDYRRMALYGIDRLIEAKKEDLHNLTGPMTDARIRLREEVAEQIKALKDMKVMGEYYGLDLSRPAYTAQ
EAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSKGTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPT
WVTESLGGRLNDGRTKVTKTSFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQSD
DYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTLKFEEVMDNYKKVLIEIARVYNE
AMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGLSIALDSLSAIKYAKVTARRNDIGLTEGFDIEGEFPCFGNDNDK
VDHLGVDLVYFFSEELKKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVA
KLRYRDSQDGISNTFSIVPKSLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLNRDMLYDAMEHPENYPQLTIRVSGYAV
NFVKLSREHQLEVISRSFHERM
>Mature_742_residues
MELNKIFKDGLWSSEINVRDFVSHNITPYYGDASFLEGPTERTKAVWNRCLEALAEERENNGVRSLDNVTVSTITSHKAG
YIDKENELIVGLQTDELLKRAIKPFGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTG
LPDNYARGRIIGDYRRMALYGIDRLIEAKKEDLHNLTGPMTDARIRLREEVAEQIKALKDMKVMGEYYGLDLSRPAYTAQ
EAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSKGTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPT
WVTESLGGRLNDGRTKVTKTSFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQSD
DYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTLKFEEVMDNYKKVLIEIARVYNE
AMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGLSIALDSLSAIKYAKVTARRNDIGLTEGFDIEGEFPCFGNDNDK
VDHLGVDLVYFFSEELKKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVA
KLRYRDSQDGISNTFSIVPKSLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLNRDMLYDAMEHPENYPQLTIRVSGYAV
NFVKLSREHQLEVISRSFHERM

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=748, Percent_Identity=57.2192513368984, Blast_Score=852, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=746, Percent_Identity=55.3619302949062, Blast_Score=825, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=580, Percent_Identity=27.2413793103448, Blast_Score=155, Evalue=8e-39,
Organism=Escherichia coli, GI1790388, Length=684, Percent_Identity=25, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1788933, Length=60, Percent_Identity=61.6666666666667, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 83793; Mature: 83793

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELNKIFKDGLWSSEINVRDFVSHNITPYYGDASFLEGPTERTKAVWNRCLEALAEEREN
CCCHHHHHCCCCCCCCCHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHC
NGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKPFGGINVVSKACHENGV
CCCCHHCCCEEEEECCCCCCCCCCCCCEEEEECHHHHHHHHHCCCCCHHHHHHHHHCCCC
EVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPDNYARGRIIGDYRRMALY
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHH
GIDRLIEAKKEDLHNLTGPMTDARIRLREEVAEQIKALKDMKVMGEYYGLDLSRPAYTAQ
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
EAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSKGTITESFAQELIDQFVIKL
HHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHH
RMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKTSFRFLQTLYNLGPSPEPNLT
HHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEE
VLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQSDDYGIACCVSYQEIGKQIQFF
EEECCCCCHHHHHHHHHCCCCCCCEEECCHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHH
GARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTLKFEEVMDNYKKVLIEIARVYNE
HHHHHHHHHHHEEECCCEECCCCEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHHHHHH
AMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGLSIALDSLSAIKYAKVTARRNDIG
HHHHHHHHHHHHHHHHHEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCC
LTEGFDIEGEFPCFGNDNDKVDHLGVDLVYFFSEELKKLPVYKNARPTLSLLTITSNVMY
CCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCEEE
GKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVAKLRYRDSQDGISNTFSIVPK
CCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
SLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLNRDMLYDAMEHPENYPQLTIRVSGYAV
HCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHCCCCCCEEEEEEECEEE
NFVKLSREHQLEVISRSFHERM
EEEEECCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELNKIFKDGLWSSEINVRDFVSHNITPYYGDASFLEGPTERTKAVWNRCLEALAEEREN
CCCHHHHHCCCCCCCCCHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHC
NGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKPFGGINVVSKACHENGV
CCCCHHCCCEEEEECCCCCCCCCCCCCEEEEECHHHHHHHHHCCCCCHHHHHHHHHCCCC
EVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPDNYARGRIIGDYRRMALY
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHH
GIDRLIEAKKEDLHNLTGPMTDARIRLREEVAEQIKALKDMKVMGEYYGLDLSRPAYTAQ
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
EAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSKGTITESFAQELIDQFVIKL
HHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHH
RMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKTSFRFLQTLYNLGPSPEPNLT
HHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEE
VLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQSDDYGIACCVSYQEIGKQIQFF
EEECCCCCHHHHHHHHHCCCCCCCEEECCHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHH
GARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTLKFEEVMDNYKKVLIEIARVYNE
HHHHHHHHHHHEEECCCEECCCCEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHHHHHH
AMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGLSIALDSLSAIKYAKVTARRNDIG
HHHHHHHHHHHHHHHHHEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCC
LTEGFDIEGEFPCFGNDNDKVDHLGVDLVYFFSEELKKLPVYKNARPTLSLLTITSNVMY
CCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCEEE
GKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVAKLRYRDSQDGISNTFSIVPK
CCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
SLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLNRDMLYDAMEHPENYPQLTIRVSGYAV
HCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHCCCCCCEEEEEEECEEE
NFVKLSREHQLEVISRSFHERM
EEEEECCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8636053 [H]