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The map label for this gene is act [H]

Identifier: 29350145

GI number: 29350145

Start: 6213943

End: 6214671

Strand: Reverse

Name: act [H]

Synonym: BT_4737

Alternate gene names: 29350145

Gene position: 6214671-6213943 (Counterclockwise)

Preceding gene: 29350146

Following gene: 29350142

Centisome position: 99.27

GC content: 49.79

Gene sequence:

>729_bases
ATGATGATAAACGTACATTCATACGAAAGTATGGGAACATTCGACGGGCCGGGCTTACGGCTCGTCGTTTTTCTTCAAGG
ATGCAACTTCCGCTGCCTCTATTGCGCCAATCCGGATACGATAGCAGGAAAGGGAGGCACGCCTACCCCACCGGAAGAAA
TCGTCCGCATGGCAATGAGCCAGCGCCCCTTCTTCGGGAAACGAGGAGGAATCACTTTCTCTGGAGGCGAACCGACGTTT
CAGGCGAAAGCACTCGTCCCGCTGGTCCGCGAACTGAAAGAAAGAGGCATCCATGTCTGTCTGGACAGCAACGGAGGACT
TTGGAACGAAGACGTAGAAGAATTATTCAAGCTGACCGATCTTGTGTTATTGGATATAAAGGAATTCAATCCCAACCGCC
ATCAGACGCTCACCGGAAGAAGCAACGAGCAAACCATCCGCACGGCCGCCTGGCTGGAAGAACAGGGAAAACCTTTCTGG
CTGCGTTATGTGCTAGTGCCCGGATACAGCGATTTTGAAGAAGACATACGCGCACTGGGAGAAGCACTCGGAAAATACAA
GATGATCCAACGGGTGGAAATACTGCCTTACCATACACTGGGCGTACATAAGTACGAAGCGATGGGACAGGAATACAAGA
TGAAAGGGGTAAAAGAAAATACCCCCGAACAGCTTGAAAAAGCTGCGGAGGTATTCAAAGAGTATTTCACTACGGTAGTA
GTTAATTAA

Upstream 100 bases:

>100_bases
GTTTCCGGTTATGCCGTAAACTTCGTGAAGTTGAGCCGCGAACACCAACTGGAAGTTATCAGCCGTAGCTTCCACGAACG
TATGTAATCTATTACTCTAT

Downstream 100 bases:

>100_bases
TGGAGAATTCTCCAATGACCTCAAAGCAAGGGCATTCTTTAATCCGTTCCCAAGGATCTACGATCCCATTATAGTTGGTG
TCGGGACTTAAATCCCGATG

Product: pyruvate formate-lyase activating enzyme

Products: NA

Alternate protein names: PFL-activating enzyme; Formate-C-acetyltransferase-activating enzyme [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKGGTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTF
QAKALVPLVRELKERGIHVCLDSNGGLWNEDVEELFKLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQGKPFW
LRYVLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTPEQLEKAAEVFKEYFTTVV
VN

Sequences:

>Translated_242_residues
MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKGGTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTF
QAKALVPLVRELKERGIHVCLDSNGGLWNEDVEELFKLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQGKPFW
LRYVLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTPEQLEKAAEVFKEYFTTVV
VN
>Mature_242_residues
MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKGGTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTF
QAKALVPLVRELKERGIHVCLDSNGGLWNEDVEELFKLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQGKPFW
LRYVLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTPEQLEKAAEVFKEYFTTVV
VN

Specific function: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=234, Percent_Identity=42.7350427350427, Blast_Score=207, Evalue=4e-55,
Organism=Escherichia coli, GI1790839, Length=268, Percent_Identity=29.4776119402985, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1790389, Length=234, Percent_Identity=26.0683760683761, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI226510931, Length=289, Percent_Identity=23.1833910034602, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 27446; Mature: 27446

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKGGTPTPPEEIVRMAMS
CEEEEECCCCCCCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHH
QRPFFGKRGGITFSGGEPTFQAKALVPLVRELKERGIHVCLDSNGGLWNEDVEELFKLTD
CCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHH
LVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQGKPFWLRYVLVPGYSDFEEDIRALG
HHEEEHHHCCCCCCEEECCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHH
EALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTPEQLEKAAEVFKEYFTTVV
HHHHHHHHHHHHHHCCCHHHCCHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHEE
VN
CC
>Mature Secondary Structure
MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKGGTPTPPEEIVRMAMS
CEEEEECCCCCCCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHH
QRPFFGKRGGITFSGGEPTFQAKALVPLVRELKERGIHVCLDSNGGLWNEDVEELFKLTD
CCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHH
LVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQGKPFWLRYVLVPGYSDFEEDIRALG
HHEEEHHHCCCCCCEEECCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHH
EALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTPEQLEKAAEVFKEYFTTVV
HHHHHHHHHHHHHHCCCHHHCCHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHEE
VN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11029425; 10899886; 12397186 [H]