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The map label for this gene is nfo

Identifier: 29350059

GI number: 29350059

Start: 6084667

End: 6085500

Strand: Reverse

Name: nfo

Synonym: BT_4651

Alternate gene names: 29350059

Gene position: 6085500-6084667 (Counterclockwise)

Preceding gene: 29350060

Following gene: 29350049

Centisome position: 97.21

GC content: 40.65

Gene sequence:

>834_bases
ATGAAATACATTGGAGCACATGTAAGCGCATCCGGCGGCGTAGAATTCGCCCCTGTCAATGCGCATGAGATTGGAGCAAA
TGCCTTTGCCCTGTTTACTAAAAATCAACGTCAATGGGTGAGTAAACCATTGAAAGAAGAAAACATCCGTCTTTTCAAAG
AAAATTGCACTAAGTACAATTTCCAAACAGACTATATTTTACCGCATGACAGTTACCTTATTAATTTGGGACACCCCGAA
GAAGAAGGATTGGAGAAAAGTCGAGCTGCCTTTTTAGATGAGATGCAACGCTGTGAACAATTAGGACTGAAACTTCTCAA
TTTCCATCCCGGCAGCCATCTGAATAAAATCTCCATAGAGGATTGTCTCGCACTCATTGCAGAAAGTATCAACCTGACTT
TGGAGAAGACTAAAGGAGTAACGGCCGTTATTGAAAATACTGCGGGGCAAGGCAGTAATTTAGGAAGTGAATTCTGGCAA
TTAAGATATATCATCGACCGGGTGAATGATAAAAGCCGTGTAGGTATTTGCCTGGATACTTGCCATACATATACAGCGGG
ATATGACATAGTGAATGATTACGATAAAGTCTTTGACGAATTTGAAAAAGAAGTAGGTTTCGAGTATCTGCGTGGTATGC
ACCTGAATGATTCTAAAAAAGAATTAGGCAGTCATGTTGACCGTCACGATAACATAGGCCAGGGACTGATCGGGAGTGCA
TTCTTTGAAAGATTAATGAAAGATTCAAGATTCGATAATATGCCACTTATTCTTGAAACACCCGATGAAAGCAAGTGGGC
AGAAGAAATTGCATGGTTGAGAAGCGTTGAATAA

Upstream 100 bases:

>100_bases
CTACTAAAAAATTGTATCTTTGCCTATGTTGGTACTCTACGCAAACATTGAAACCTGCATAAATGTTATATCAACAGATA
AAATTTAAATAGAATAAAAG

Downstream 100 bases:

>100_bases
ACGCTTCACTTCTTTTTTAATAAATATTCGCCATACTGACTAACGGCTCATAGAGTTTCTCGATATCCTGCGAGTCCATT
TCTATCTGCACCTGATCCCC

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MKYIGAHVSASGGVEFAPVNAHEIGANAFALFTKNQRQWVSKPLKEENIRLFKENCTKYNFQTDYILPHDSYLINLGHPE
EEGLEKSRAAFLDEMQRCEQLGLKLLNFHPGSHLNKISIEDCLALIAESINLTLEKTKGVTAVIENTAGQGSNLGSEFWQ
LRYIIDRVNDKSRVGICLDTCHTYTAGYDIVNDYDKVFDEFEKEVGFEYLRGMHLNDSKKELGSHVDRHDNIGQGLIGSA
FFERLMKDSRFDNMPLILETPDESKWAEEIAWLRSVE

Sequences:

>Translated_277_residues
MKYIGAHVSASGGVEFAPVNAHEIGANAFALFTKNQRQWVSKPLKEENIRLFKENCTKYNFQTDYILPHDSYLINLGHPE
EEGLEKSRAAFLDEMQRCEQLGLKLLNFHPGSHLNKISIEDCLALIAESINLTLEKTKGVTAVIENTAGQGSNLGSEFWQ
LRYIIDRVNDKSRVGICLDTCHTYTAGYDIVNDYDKVFDEFEKEVGFEYLRGMHLNDSKKELGSHVDRHDNIGQGLIGSA
FFERLMKDSRFDNMPLILETPDESKWAEEIAWLRSVE
>Mature_277_residues
MKYIGAHVSASGGVEFAPVNAHEIGANAFALFTKNQRQWVSKPLKEENIRLFKENCTKYNFQTDYILPHDSYLINLGHPE
EEGLEKSRAAFLDEMQRCEQLGLKLLNFHPGSHLNKISIEDCLALIAESINLTLEKTKGVTAVIENTAGQGSNLGSEFWQ
LRYIIDRVNDKSRVGICLDTCHTYTAGYDIVNDYDKVFDEFEKEVGFEYLRGMHLNDSKKELGSHVDRHDNIGQGLIGSA
FFERLMKDSRFDNMPLILETPDESKWAEEIAWLRSVE

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=277, Percent_Identity=64.9819494584838, Blast_Score=374, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17531193, Length=271, Percent_Identity=50.1845018450184, Blast_Score=285, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6322735, Length=281, Percent_Identity=42.7046263345196, Blast_Score=231, Evalue=7e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_BACTN (Q89YT0)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_813562.1
- ProteinModelPortal:   Q89YT0
- SMR:   Q89YT0
- GeneID:   1073121
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_4651
- NMPDR:   fig|226186.1.peg.4649
- HOGENOM:   HBG565018
- OMA:   QIALETM
- PhylomeDB:   Q89YT0
- ProtClustDB:   PRK01060
- BioCyc:   BTHE226186:BT_4651-MONOMER
- BRENDA:   3.1.21.2
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 31540; Mature: 31540

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIGAHVSASGGVEFAPVNAHEIGANAFALFTKNQRQWVSKPLKEENIRLFKENCTKYN
CCEECCEEECCCCEEEEECCHHHHCCCEEEEEECCHHHHHHCCCCHHHHHHHHHCCCEEC
FQTDYILPHDSYLINLGHPEEEGLEKSRAAFLDEMQRCEQLGLKLLNFHPGSHLNKISIE
CCCCEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHH
DCLALIAESINLTLEKTKGVTAVIENTAGQGSNLGSEFWQLRYIIDRVNDKSRVGICLDT
HHHHHHHHHHCEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEHH
CHTYTAGYDIVNDYDKVFDEFEKEVGFEYLRGMHLNDSKKELGSHVDRHDNIGQGLIGSA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHH
FFERLMKDSRFDNMPLILETPDESKWAEEIAWLRSVE
HHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKYIGAHVSASGGVEFAPVNAHEIGANAFALFTKNQRQWVSKPLKEENIRLFKENCTKYN
CCEECCEEECCCCEEEEECCHHHHCCCEEEEEECCHHHHHHCCCCHHHHHHHHHCCCEEC
FQTDYILPHDSYLINLGHPEEEGLEKSRAAFLDEMQRCEQLGLKLLNFHPGSHLNKISIE
CCCCEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHH
DCLALIAESINLTLEKTKGVTAVIENTAGQGSNLGSEFWQLRYIIDRVNDKSRVGICLDT
HHHHHHHHHHCEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEHH
CHTYTAGYDIVNDYDKVFDEFEKEVGFEYLRGMHLNDSKKELGSHVDRHDNIGQGLIGSA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHH
FFERLMKDSRFDNMPLILETPDESKWAEEIAWLRSVE
HHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928