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The map label for this gene is clpB

Identifier: 29350005

GI number: 29350005

Start: 6016876

End: 6019464

Strand: Reverse

Name: clpB

Synonym: BT_4597

Alternate gene names: 29350005

Gene position: 6019464-6016876 (Counterclockwise)

Preceding gene: 29350008

Following gene: 29349998

Centisome position: 96.15

GC content: 43.18

Gene sequence:

>2589_bases
ATGAACTTTAACAATTTTACCATCAAATCTCAAGAAGCGGTACAGGAGGCCGTAAATCTCGTACAAAGTCGGGGACAACA
GGCGATTGAACCTGCTCATATCCTACATGGAGTAATGAAGGTAGGCGAAAATGTGACTAACTTCATCTTTCAAAAATTAG
GAATGAACGGGCAACAGGTAGCTCTAGTCGTTGACAAACAGATCGAATCTCTGCCTAAAGTATCCGGAGGAGAACCTTAC
CTAAGCAGAGAATCTAATGAAGTATTGCAGAAGGCAACCCAGTATTCTAAAGAAATGGGAGATGAATTTGTTTCATTAGA
ACATATTCTACTGGCTTTGCTAACTGTGAAAAGTACCGTATCCACCATCTTGAAAGATGCCGGAATGACGGAAAAAGAAT
TGCGTAACGCTATCAATGAACTCAGAAAAGGAGAAAAAGTTACTTCTCAATCCAGTGAAGACAACTATCAGTCATTGGAG
AAATACGCTATCAATCTGAACGAAGCCGCCCGCAGCGGCAAACTGGACCCTGTAATCGGACGTGACGAAGAAATCCGACG
GGTACTGCAAATATTAAGCCGCCGAACCAAGAACAATCCGATCTTGATTGGTGAACCGGGTACAGGTAAGACAGCTATCG
TAGAAGGATTGGCGCATCGTATCCTCCGGGGAGACGTACCCGAAAACCTGAAAAACAAACAGGTCTACTCTCTCGATATG
GGAGCATTGGTTGCAGGAGCAAAATATAAAGGAGAATTCGAAGAGCGACTAAAATCAGTAGTCAATGAGGTTAAGAAATC
AGAAGGTGATATAATCTTGTTTATTGATGAGATTCATACACTGGTAGGCGCCGGCAAAGGCGAAGGAGCAATGGATGCTG
CAAATATTCTGAAACCGGCATTGGCACGTGGAGAACTACGTTCGATCGGTGCAACCACTCTCGACGAATATCAGAAGTAT
TTCGAGAAAGATAAAGCATTGGAACGTCGTTTCCAGATTGTACAGGTAGATGAACCGGATACTCTGAGTACCATTTCCAT
CCTTCGTGGATTGAAAGAACGCTACGAGAACCATCATCATGTACGTATCAAAGACGATGCCATCATAGCAGCTGTAGAAC
TGAGCAGCCGTTACATCACTGACCGTTTCTTGCCGGACAAAGCAATCGACCTGATGGATGAAGCAGCAGCCAAACTACGT
ATGGAAGTAGATTCCGTTCCAGAAGAATTGGATGAAATCTCACGTAAGATCAAACAGTTAGAAATCGAACGTGAAGCCAT
TAAGCGTGAAAATGATAAGCCGAAACTGGAAATTATCGGCAAAGAACTGGCCGAACTAAAAGAAGTTGAGAAGTCCTTCA
AAGCTAAATGGCAAAGTGAAAAAACGCTGATGGATAAAATCCAGCAAAACAAAGTGGAAATAGAGAATCTGAAGTTCGAG
GCAGAGAAAGCGGAACGTGAAGGAGACTATGGAAAAGTTGCCGAAATCCGTTACGGAAAGCTCCAGGCTTTGGACAAAGA
GATAGAAGATACGCAGGAGAAACTTCGTGGTATGCAAGGTGATAAAGCCATGATCAAAGAAGAAGTGGATGCTGAAGACA
TCGCTGACGTTGTTTCACGCTGGACGGGCATCCCTGTCAGCAAAATGATGCAAAGTGAGAAAGATAAATTATTGCATTTG
GAAGACGAACTCCATCAACGAGTGATTGGACAGGACGAAGCAATTGAAGCGGTTGCAGATGCCGTACGTCGTAGCCGTGC
CGGATTACAGGATCCCAAACGCCCGATCGGTTCTTTTATTTTCCTTGGAACGACCGGTGTCGGTAAAACTGAACTGGCAA
AGGCATTGGCGGAATTCCTCTTTGACGATGAATCGATGATGACACGTATAGATATGAGCGAGTATCAAGAAAAGCATAGT
GTTTCTCGTTTGGTCGGAGCACCTCCGGGATACGTTGGATACGATGAAGGCGGCCAGCTGACAGAAGCCATTCGCCGGAA
ACCTTACTCAGTTGTTTTGTTTGACGAAATAGAAAAAGCGCATCCGGATGTTTTCAATATCCTGTTGCAAGTACTGGATG
ACGGACGACTCACTGACAACAAAGGAAGAGTAGTCAACTTCAAAAACACCATCATCATCATGACTTCAAATATGGGAAGT
TCTTATATACAAAGTCAGATGGAGAAACTGAGCGGTTCAAATAAGGAAGAAGTTATCGAAGAAACGAAGAAGGAAGTGAT
GAATATGCTAAAGAAAAACATTCGTCCGGAATTCCTGAATCGTATTGATGAAACAATCATGTTCCTGCCGCTGACGGAAA
CTGAAATCAGACAAATTGTACTCTTACAAATCAAAGGTGTACAAAAGATGCTTGCAGAAAACGGAGTTGAACTGGAAATG
ACAGATGCTGCTTTGAATTTCTTATCCCAGGTAGGATATGATCCTGAATTTGGAGCCCGTCCGGTAAAAAGAGCTATCCA
GCGTTATTTACTGAACGATCTGTCGAAAAAGTTATTGTCTCAGGAAGTCGACAGAAGCAAAGCAATCATTGTTGACTCAA
ACGGAGACGGATTAGTATTCAGAAACTAA

Upstream 100 bases:

>100_bases
TTTATAATCATGTTATTTTGTCATATTTTTCTACTTGGCAAATCTTTTGAGCCCCTTCAAGTGTTATTAAAATCATGAAA
AAAGAAAGGAGAACAACCAT

Downstream 100 bases:

>100_bases
ATTATAGTTAGTTGTTATAAGGACAGCCGGTATGTTCCCAAGTGAGCATATCGGCTTTTTTATATTCCTGAATATATAAT
TCTTCTTACTTCCGGCTGAC

Product: endopeptidase Clp ATP-binding subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 862; Mature: 862

Protein sequence:

>862_residues
MNFNNFTIKSQEAVQEAVNLVQSRGQQAIEPAHILHGVMKVGENVTNFIFQKLGMNGQQVALVVDKQIESLPKVSGGEPY
LSRESNEVLQKATQYSKEMGDEFVSLEHILLALLTVKSTVSTILKDAGMTEKELRNAINELRKGEKVTSQSSEDNYQSLE
KYAINLNEAARSGKLDPVIGRDEEIRRVLQILSRRTKNNPILIGEPGTGKTAIVEGLAHRILRGDVPENLKNKQVYSLDM
GALVAGAKYKGEFEERLKSVVNEVKKSEGDIILFIDEIHTLVGAGKGEGAMDAANILKPALARGELRSIGATTLDEYQKY
FEKDKALERRFQIVQVDEPDTLSTISILRGLKERYENHHHVRIKDDAIIAAVELSSRYITDRFLPDKAIDLMDEAAAKLR
MEVDSVPEELDEISRKIKQLEIEREAIKRENDKPKLEIIGKELAELKEVEKSFKAKWQSEKTLMDKIQQNKVEIENLKFE
AEKAEREGDYGKVAEIRYGKLQALDKEIEDTQEKLRGMQGDKAMIKEEVDAEDIADVVSRWTGIPVSKMMQSEKDKLLHL
EDELHQRVIGQDEAIEAVADAVRRSRAGLQDPKRPIGSFIFLGTTGVGKTELAKALAEFLFDDESMMTRIDMSEYQEKHS
VSRLVGAPPGYVGYDEGGQLTEAIRRKPYSVVLFDEIEKAHPDVFNILLQVLDDGRLTDNKGRVVNFKNTIIIMTSNMGS
SYIQSQMEKLSGSNKEEVIEETKKEVMNMLKKNIRPEFLNRIDETIMFLPLTETEIRQIVLLQIKGVQKMLAENGVELEM
TDAALNFLSQVGYDPEFGARPVKRAIQRYLLNDLSKKLLSQEVDRSKAIIVDSNGDGLVFRN

Sequences:

>Translated_862_residues
MNFNNFTIKSQEAVQEAVNLVQSRGQQAIEPAHILHGVMKVGENVTNFIFQKLGMNGQQVALVVDKQIESLPKVSGGEPY
LSRESNEVLQKATQYSKEMGDEFVSLEHILLALLTVKSTVSTILKDAGMTEKELRNAINELRKGEKVTSQSSEDNYQSLE
KYAINLNEAARSGKLDPVIGRDEEIRRVLQILSRRTKNNPILIGEPGTGKTAIVEGLAHRILRGDVPENLKNKQVYSLDM
GALVAGAKYKGEFEERLKSVVNEVKKSEGDIILFIDEIHTLVGAGKGEGAMDAANILKPALARGELRSIGATTLDEYQKY
FEKDKALERRFQIVQVDEPDTLSTISILRGLKERYENHHHVRIKDDAIIAAVELSSRYITDRFLPDKAIDLMDEAAAKLR
MEVDSVPEELDEISRKIKQLEIEREAIKRENDKPKLEIIGKELAELKEVEKSFKAKWQSEKTLMDKIQQNKVEIENLKFE
AEKAEREGDYGKVAEIRYGKLQALDKEIEDTQEKLRGMQGDKAMIKEEVDAEDIADVVSRWTGIPVSKMMQSEKDKLLHL
EDELHQRVIGQDEAIEAVADAVRRSRAGLQDPKRPIGSFIFLGTTGVGKTELAKALAEFLFDDESMMTRIDMSEYQEKHS
VSRLVGAPPGYVGYDEGGQLTEAIRRKPYSVVLFDEIEKAHPDVFNILLQVLDDGRLTDNKGRVVNFKNTIIIMTSNMGS
SYIQSQMEKLSGSNKEEVIEETKKEVMNMLKKNIRPEFLNRIDETIMFLPLTETEIRQIVLLQIKGVQKMLAENGVELEM
TDAALNFLSQVGYDPEFGARPVKRAIQRYLLNDLSKKLLSQEVDRSKAIIVDSNGDGLVFRN
>Mature_862_residues
MNFNNFTIKSQEAVQEAVNLVQSRGQQAIEPAHILHGVMKVGENVTNFIFQKLGMNGQQVALVVDKQIESLPKVSGGEPY
LSRESNEVLQKATQYSKEMGDEFVSLEHILLALLTVKSTVSTILKDAGMTEKELRNAINELRKGEKVTSQSSEDNYQSLE
KYAINLNEAARSGKLDPVIGRDEEIRRVLQILSRRTKNNPILIGEPGTGKTAIVEGLAHRILRGDVPENLKNKQVYSLDM
GALVAGAKYKGEFEERLKSVVNEVKKSEGDIILFIDEIHTLVGAGKGEGAMDAANILKPALARGELRSIGATTLDEYQKY
FEKDKALERRFQIVQVDEPDTLSTISILRGLKERYENHHHVRIKDDAIIAAVELSSRYITDRFLPDKAIDLMDEAAAKLR
MEVDSVPEELDEISRKIKQLEIEREAIKRENDKPKLEIIGKELAELKEVEKSFKAKWQSEKTLMDKIQQNKVEIENLKFE
AEKAEREGDYGKVAEIRYGKLQALDKEIEDTQEKLRGMQGDKAMIKEEVDAEDIADVVSRWTGIPVSKMMQSEKDKLLHL
EDELHQRVIGQDEAIEAVADAVRRSRAGLQDPKRPIGSFIFLGTTGVGKTELAKALAEFLFDDESMMTRIDMSEYQEKHS
VSRLVGAPPGYVGYDEGGQLTEAIRRKPYSVVLFDEIEKAHPDVFNILLQVLDDGRLTDNKGRVVNFKNTIIIMTSNMGS
SYIQSQMEKLSGSNKEEVIEETKKEVMNMLKKNIRPEFLNRIDETIMFLPLTETEIRQIVLLQIKGVQKMLAENGVELEM
TDAALNFLSQVGYDPEFGARPVKRAIQRYLLNDLSKKLLSQEVDRSKAIIVDSNGDGLVFRN

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family

Homologues:

Organism=Homo sapiens, GI13540606, Length=302, Percent_Identity=38.4105960264901, Blast_Score=213, Evalue=5e-55,
Organism=Escherichia coli, GI1788943, Length=865, Percent_Identity=53.6416184971098, Blast_Score=928, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=394, Percent_Identity=40.8629441624365, Blast_Score=287, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6320464, Length=691, Percent_Identity=53.4008683068017, Blast_Score=728, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=856, Percent_Identity=42.6401869158879, Blast_Score=670, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CLPB_BACTN (Q89YY3)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_813508.1
- ProteinModelPortal:   Q89YY3
- GeneID:   1073545
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_4597
- NMPDR:   fig|226186.1.peg.4595
- HOGENOM:   HBG413133
- OMA:   YITERRL
- PhylomeDB:   Q89YY3
- ProtClustDB:   CLSK913659
- BioCyc:   BTHE226186:BT_4597-MONOMER
- GO:   GO:0005737
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150
- Gene3D:   G3DSA:1.10.1780.10
- PRINTS:   PR00300
- SMART:   SM00382
- TIGRFAMs:   TIGR03346

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 97190; Mature: 97190

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00870 CLPAB_1; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFNNFTIKSQEAVQEAVNLVQSRGQQAIEPAHILHGVMKVGENVTNFIFQKLGMNGQQV
CCCCCCEECCHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCEE
ALVVDKQIESLPKVSGGEPYLSRESNEVLQKATQYSKEMGDEFVSLEHILLALLTVKSTV
EEEECHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STILKDAGMTEKELRNAINELRKGEKVTSQSSEDNYQSLEKYAINLNEAARSGKLDPVIG
HHHHHHCCCCHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHCHHHHHHCCCCCCCCC
RDEEIRRVLQILSRRTKNNPILIGEPGTGKTAIVEGLAHRILRGDVPENLKNKQVYSLDM
CCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECH
GALVAGAKYKGEFEERLKSVVNEVKKSEGDIILFIDEIHTLVGAGKGEGAMDAANILKPA
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCHHHHHHHHHH
LARGELRSIGATTLDEYQKYFEKDKALERRFQIVQVDEPDTLSTISILRGLKERYENHHH
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHCCCCE
VRIKDDAIIAAVELSSRYITDRFLPDKAIDLMDEAAAKLRMEVDSVPEELDEISRKIKQL
EEEECCCEEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIEREAIKRENDKPKLEIIGKELAELKEVEKSFKAKWQSEKTLMDKIQQNKVEIENLKFE
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
AEKAEREGDYGKVAEIRYGKLQALDKEIEDTQEKLRGMQGDKAMIKEEVDAEDIADVVSR
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHH
WTGIPVSKMMQSEKDKLLHLEDELHQRVIGQDEAIEAVADAVRRSRAGLQDPKRPIGSFI
HCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCEE
FLGTTGVGKTELAKALAEFLFDDESMMTRIDMSEYQEKHSVSRLVGAPPGYVGYDEGGQL
EEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
TEAIRRKPYSVVLFDEIEKAHPDVFNILLQVLDDGRLTDNKGRVVNFKNTIIIMTSNMGS
HHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCEECCCCCEEEECCEEEEEECCCCH
SYIQSQMEKLSGSNKEEVIEETKKEVMNMLKKNIRPEFLNRIDETIMFLPLTETEIRQIV
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEECCHHHHHHHH
LLQIKGVQKMLAENGVELEMTDAALNFLSQVGYDPEFGARPVKRAIQRYLLNDLSKKLLS
HHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QEVDRSKAIIVDSNGDGLVFRN
HHHCCCCEEEEECCCCEEEECC
>Mature Secondary Structure
MNFNNFTIKSQEAVQEAVNLVQSRGQQAIEPAHILHGVMKVGENVTNFIFQKLGMNGQQV
CCCCCCEECCHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCEE
ALVVDKQIESLPKVSGGEPYLSRESNEVLQKATQYSKEMGDEFVSLEHILLALLTVKSTV
EEEECHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STILKDAGMTEKELRNAINELRKGEKVTSQSSEDNYQSLEKYAINLNEAARSGKLDPVIG
HHHHHHCCCCHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHCHHHHHHCCCCCCCCC
RDEEIRRVLQILSRRTKNNPILIGEPGTGKTAIVEGLAHRILRGDVPENLKNKQVYSLDM
CCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECH
GALVAGAKYKGEFEERLKSVVNEVKKSEGDIILFIDEIHTLVGAGKGEGAMDAANILKPA
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCHHHHHHHHHH
LARGELRSIGATTLDEYQKYFEKDKALERRFQIVQVDEPDTLSTISILRGLKERYENHHH
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHCCCCE
VRIKDDAIIAAVELSSRYITDRFLPDKAIDLMDEAAAKLRMEVDSVPEELDEISRKIKQL
EEEECCCEEEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIEREAIKRENDKPKLEIIGKELAELKEVEKSFKAKWQSEKTLMDKIQQNKVEIENLKFE
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
AEKAEREGDYGKVAEIRYGKLQALDKEIEDTQEKLRGMQGDKAMIKEEVDAEDIADVVSR
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHH
WTGIPVSKMMQSEKDKLLHLEDELHQRVIGQDEAIEAVADAVRRSRAGLQDPKRPIGSFI
HCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCEE
FLGTTGVGKTELAKALAEFLFDDESMMTRIDMSEYQEKHSVSRLVGAPPGYVGYDEGGQL
EEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
TEAIRRKPYSVVLFDEIEKAHPDVFNILLQVLDDGRLTDNKGRVVNFKNTIIIMTSNMGS
HHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCEECCCCCEEEECCEEEEEECCCCH
SYIQSQMEKLSGSNKEEVIEETKKEVMNMLKKNIRPEFLNRIDETIMFLPLTETEIRQIV
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEECCHHHHHHHH
LLQIKGVQKMLAENGVELEMTDAALNFLSQVGYDPEFGARPVKRAIQRYLLNDLSKKLLS
HHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QEVDRSKAIIVDSNGDGLVFRN
HHHCCCCEEEEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928