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The map label for this gene is trmE

Identifier: 29349959

GI number: 29349959

Start: 5971982

End: 5973379

Strand: Reverse

Name: trmE

Synonym: BT_4551

Alternate gene names: 29349959

Gene position: 5973379-5971982 (Counterclockwise)

Preceding gene: 29349962

Following gene: 29349958

Centisome position: 95.42

GC content: 42.49

Gene sequence:

>1398_bases
ATGAATCAAGATACTATCTGCGCTATTGCTACCGCTCAAGGAGGGGCCATCGGAAGCATCCGTGTTTCCGGTCCTGAAGC
TATTTCTATTACAAGCCGCATCTTCCAACCGGCCAAAGCCGGCAAATTACTTAGCGAACAAAAGCCTTATACGCTGACAT
TCGGGCGTATTTATAATGGAGAAGAAGTCATAGACGAAGTCCTTGTGAGTCTTTTCCGGGCTCCTCATTCCTATACGGGA
GAGGATAGTACAGAAATCACGTGTCATGGTTCTTCCTATATTCTTCAGCAAGTAATGCAGTTGTTGATTAAGAACGGTTG
CCGTATGGCTCAACCGGGAGAATATACCCAACGCGCGTTCCTCAACGGAAAGATGGACTTAAGTCAGGCGGAGGCTGTAG
CCGATTTGATTGCATCTTCTTCCGCAGCCACTCATCGTTTGGCCATGAGTCAGATGCGGGGAGGTTTTAGCAAAGAGTTA
ACGGACCTACGAAGTAAGTTGCTAAACTTCACTTCCATGATCGAACTGGAACTTGACTTCAGCGAAGAAGATGTGGAGTT
TGCTGATCGTTCGGCATTGCGAAAACTCGCAGATGAAATAGAGCAGGTTATCTCACGGCTGGTTCATTCATTCAATGTAG
GGAATGCCATTAAGAATGGTGTTCCGGTGGCTATCATCGGTGAAACGAATGCGGGGAAATCTACTTTACTGAATGTATTA
CTGAACGAGGATAAAGCAATCGTCAGTGATATTCATGGTACCACGCGTGATGTCATTGAAGATACTATTAATATAGGTGG
CATCACTTTCCGATTCATTGATACTGCCGGAATCCGGGAAACAAATGATACCATTGAAAGCCTTGGTATTGAACGTACAT
TCCAGAAGTTGGATCAAGCGGAGATTGTACTTTGGATGGTTGATTCATCTGATGCATCTTCTCAGATAAAGCAATTATCA
GAAAAGATCATTCCTCGTTGTGAAGAGAAGCAACTAATTGTTGTATTCAACAAAGCGGATTTGATTGAGGAAATGCAGAA
AGAAGAATTATCTGCTTTATTAAAGAACTTCCCTAAAGAATACACGAAGTCTATCTTCATATCGGCAAAAGAACGTAGAC
AGACCGACGAGTTACAAAAGATGCTTATTAACGCAGCACATCTGCCTACGGTAACACAAAATGATATTATTGTGACAAAC
GTAAGGCATTATGAAGCTTTAAGTAAAGCTTTAGATGCTATCCACCGGGTGCAGGATGGGCTTGATTCACATATATCCGG
AGATTTTCTTTCGCAGGATATACGAGAGTGCATTTTCTTTATTTCGGATATTGCAGGGGAAGTGACAAATGATATGGTGC
TTCAGAATATTTTTCAGCATTTTTGCATCGGCAAATGA

Upstream 100 bases:

>100_bases
TGAACGGCAGCAAATATAAACAAATAAAGCACAAGTTGTGTCTAATCTTACTTTTTTTACTTAGAAACAATTATCTTTGC
ATCATCCAAAAAGAAATATA

Downstream 100 bases:

>100_bases
CGGCTTGTAAACAGCCATAAATAACCATAGCCAATTAGAAGAAAGTCGCTGATAACCAGCGGCTTTTCTTTTTATATGTT
TTTCAGTCAAATCTGACTGG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MNQDTICAIATAQGGAIGSIRVSGPEAISITSRIFQPAKAGKLLSEQKPYTLTFGRIYNGEEVIDEVLVSLFRAPHSYTG
EDSTEITCHGSSYILQQVMQLLIKNGCRMAQPGEYTQRAFLNGKMDLSQAEAVADLIASSSAATHRLAMSQMRGGFSKEL
TDLRSKLLNFTSMIELELDFSEEDVEFADRSALRKLADEIEQVISRLVHSFNVGNAIKNGVPVAIIGETNAGKSTLLNVL
LNEDKAIVSDIHGTTRDVIEDTINIGGITFRFIDTAGIRETNDTIESLGIERTFQKLDQAEIVLWMVDSSDASSQIKQLS
EKIIPRCEEKQLIVVFNKADLIEEMQKEELSALLKNFPKEYTKSIFISAKERRQTDELQKMLINAAHLPTVTQNDIIVTN
VRHYEALSKALDAIHRVQDGLDSHISGDFLSQDIRECIFFISDIAGEVTNDMVLQNIFQHFCIGK

Sequences:

>Translated_465_residues
MNQDTICAIATAQGGAIGSIRVSGPEAISITSRIFQPAKAGKLLSEQKPYTLTFGRIYNGEEVIDEVLVSLFRAPHSYTG
EDSTEITCHGSSYILQQVMQLLIKNGCRMAQPGEYTQRAFLNGKMDLSQAEAVADLIASSSAATHRLAMSQMRGGFSKEL
TDLRSKLLNFTSMIELELDFSEEDVEFADRSALRKLADEIEQVISRLVHSFNVGNAIKNGVPVAIIGETNAGKSTLLNVL
LNEDKAIVSDIHGTTRDVIEDTINIGGITFRFIDTAGIRETNDTIESLGIERTFQKLDQAEIVLWMVDSSDASSQIKQLS
EKIIPRCEEKQLIVVFNKADLIEEMQKEELSALLKNFPKEYTKSIFISAKERRQTDELQKMLINAAHLPTVTQNDIIVTN
VRHYEALSKALDAIHRVQDGLDSHISGDFLSQDIRECIFFISDIAGEVTNDMVLQNIFQHFCIGK
>Mature_465_residues
MNQDTICAIATAQGGAIGSIRVSGPEAISITSRIFQPAKAGKLLSEQKPYTLTFGRIYNGEEVIDEVLVSLFRAPHSYTG
EDSTEITCHGSSYILQQVMQLLIKNGCRMAQPGEYTQRAFLNGKMDLSQAEAVADLIASSSAATHRLAMSQMRGGFSKEL
TDLRSKLLNFTSMIELELDFSEEDVEFADRSALRKLADEIEQVISRLVHSFNVGNAIKNGVPVAIIGETNAGKSTLLNVL
LNEDKAIVSDIHGTTRDVIEDTINIGGITFRFIDTAGIRETNDTIESLGIERTFQKLDQAEIVLWMVDSSDASSQIKQLS
EKIIPRCEEKQLIVVFNKADLIEEMQKEELSALLKNFPKEYTKSIFISAKERRQTDELQKMLINAAHLPTVTQNDIIVTN
VRHYEALSKALDAIHRVQDGLDSHISGDFLSQDIRECIFFISDIAGEVTNDMVLQNIFQHFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=479, Percent_Identity=30.2713987473904, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI193082988, Length=506, Percent_Identity=28.2608695652174, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI193082993, Length=471, Percent_Identity=28.8747346072187, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI2367268, Length=471, Percent_Identity=34.3949044585987, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI87082120, Length=137, Percent_Identity=32.8467153284672, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17507259, Length=469, Percent_Identity=29.2110874200426, Blast_Score=171, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6323665, Length=506, Percent_Identity=29.4466403162055, Blast_Score=176, Evalue=8e-45,
Organism=Drosophila melanogaster, GI45550813, Length=490, Percent_Identity=30.6122448979592, Blast_Score=189, Evalue=3e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_BACTN (Q89Z26)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_813462.1
- ProteinModelPortal:   Q89Z26
- SMR:   Q89Z26
- GeneID:   1072889
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_4551
- NMPDR:   fig|226186.1.peg.4549
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- PhylomeDB:   Q89Z26
- ProtClustDB:   PRK05291
- BioCyc:   BTHE226186:BT_4551-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 51612; Mature: 51612

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: BINDING 21-21 BINDING 85-85 BINDING 124-124 BINDING 465-465

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQDTICAIATAQGGAIGSIRVSGPEAISITSRIFQPAKAGKLLSEQKPYTLTFGRIYNG
CCCCCEEEEEECCCCCEEEEEECCCCHHHHHHHHHCHHHHHHHHHCCCCCEEEECEECCC
EEVIDEVLVSLFRAPHSYTGEDSTEITCHGSSYILQQVMQLLIKNGCRMAQPGEYTQRAF
HHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
LNGKMDLSQAEAVADLIASSSAATHRLAMSQMRGGFSKELTDLRSKLLNFTSMIELELDF
HCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEEECC
SEEDVEFADRSALRKLADEIEQVISRLVHSFNVGNAIKNGVPVAIIGETNAGKSTLLNVL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEECCCCCHHHHHHHH
LNEDKAIVSDIHGTTRDVIEDTINIGGITFRFIDTAGIRETNDTIESLGIERTFQKLDQA
HCCCHHHHHHHCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC
EIVLWMVDSSDASSQIKQLSEKIIPRCEEKQLIVVFNKADLIEEMQKEELSALLKNFPKE
EEEEEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCHH
YTKSIFISAKERRQTDELQKMLINAAHLPTVTQNDIIVTNVRHYEALSKALDAIHRVQDG
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHH
LDSHISGDFLSQDIRECIFFISDIAGEVTNDMVLQNIFQHFCIGK
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNQDTICAIATAQGGAIGSIRVSGPEAISITSRIFQPAKAGKLLSEQKPYTLTFGRIYNG
CCCCCEEEEEECCCCCEEEEEECCCCHHHHHHHHHCHHHHHHHHHCCCCCEEEECEECCC
EEVIDEVLVSLFRAPHSYTGEDSTEITCHGSSYILQQVMQLLIKNGCRMAQPGEYTQRAF
HHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
LNGKMDLSQAEAVADLIASSSAATHRLAMSQMRGGFSKELTDLRSKLLNFTSMIELELDF
HCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEEECC
SEEDVEFADRSALRKLADEIEQVISRLVHSFNVGNAIKNGVPVAIIGETNAGKSTLLNVL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEECCCCCHHHHHHHH
LNEDKAIVSDIHGTTRDVIEDTINIGGITFRFIDTAGIRETNDTIESLGIERTFQKLDQA
HCCCHHHHHHHCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCC
EIVLWMVDSSDASSQIKQLSEKIIPRCEEKQLIVVFNKADLIEEMQKEELSALLKNFPKE
EEEEEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCHH
YTKSIFISAKERRQTDELQKMLINAAHLPTVTQNDIIVTNVRHYEALSKALDAIHRVQDG
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHH
LDSHISGDFLSQDIRECIFFISDIAGEVTNDMVLQNIFQHFCIGK
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928