Click here to switch to the map view.

The map label for this gene is tnpA [H]

Identifier: 29349800

GI number: 29349800

Start: 5784621

End: 5785886

Strand: Reverse

Name: tnpA [H]

Synonym: BT_4392

Alternate gene names: 29349800

Gene position: 5785886-5784621 (Counterclockwise)

Preceding gene: 29349801

Following gene: 29349791

Centisome position: 92.42

GC content: 42.1

Gene sequence:

>1266_bases
ATGGGTGCTTATAGATTTTTGAACAATTCCTCAGTCAGCTCTGATGCTATCTTATCAGGTCTGATACACACTTGCTGTAA
GAATGCTTCCGGTCGTCAGCATTTACTGTGTATTCAAGATACCTCGGAGATAAACTATGAGGCTCATGTTGAGCGAATGA
AGAAAAAAACAGCCAGTCCCGGCATTGTCGGTCAAAAGCAATGTGGTACTTTTTTGCATCCTGTCTTGGTAGTGGATGCC
TCCAGTCATATTCCTATAGGCTTTTCTTCGGTTAAGCAGTGGAATCGCTCACCGGCTGCTTTAAGTCGTGAAGAACGTAA
TTACAGATATCAGCCTATAGAAGAAAAAGAGTCATATCGTTGGATAGAAAGTGGAATGGCTGCCAGTGAGCAAATGCCCC
GGGATGCAGTTAAAACGATCATCGGTGACCGTGAAGCGGACATTTTCGAGCTTTTCAGCCGTATCCCTACTGATAATGTT
CACTTGCTGATACGTTCTGTTCATGAAAGGAATTGCCGGTTGGATGATCCAGACTGTTCTGTCCATCTGAATACATTAAT
GGAGCAGGCTGTTCTACGGGCAGAGTATAGCTTTGAAGTGCTCCCGGGAAGCGGACGTAAGAAACGGGTAGCGTGCATGG
AACTTCGCTTTGAAAGAGTCACCTTGTGCGCTCCTGTTAACGGTCCGGCAAAGGGCAGTCCCCCTGTTAGTCTTTATTGC
ATACATGTTAAAGAAAAATCTTCCAGTACACCGGTAAATGAGAGCCCTATTGAATGGAGACTGCTAACTACACATGTGGT
GGAGACTGTAGAACAAGCAATTGAATGTATCGGTTGGTATCGTTGTAGATGGCTGATTGAAGAGTTGTTCAGAGTGCTCA
AAAGAAAGGGATTCATGATTGAGGACGCACAGTTGGAAACAGTTTCGGCATTACAAAAACTAATCTTAATTTCCTTGCAG
GCAGCCCTGCAGGTGATGGTACTCAAACTTTCTTTTGATAAAGAAGATGAAAAACTCTCTTCAGAAATCTACTTTACAAG
CAAAGAAATAGCATTATTACATATAGTAGGAAAAAAGAGTGAGGGAAATACAAAAATACAGCAAAATCCATATAAAAAAG
AATCAATGGCATGGGCAGCATGGATTATTGCAAGGTTAGGAGCATGGAGTGCATACAAGAGCCAGTCCATTCCAGGATAT
ATTACCTTTAAGAATGGACTGGATAGATTTTATACACAGTTTGAACTGTATGAGTTAATCAGCTAA

Upstream 100 bases:

>100_bases
TGATCCTCGGCTTTTGCGGCGTTTAAACTTAATTTGCTCTCAGATGGTTGTCCATCAAAGTGCTATAGTGAATCAATTTA
GTAAGGAACATAAAGAAAAG

Downstream 100 bases:

>100_bases
GACTTGTGTATAAAGGGTAGCTTCGGGAAGGAGGAGTACCCGCAGGGGGAGGTGGTAGGTGAATACGCAGTCCACTTATT
CACTGCAAATTATAGACAAC

Product: putative Tn5 transposase

Products: NA

Alternate protein names: Tnp [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MGAYRFLNNSSVSSDAILSGLIHTCCKNASGRQHLLCIQDTSEINYEAHVERMKKKTASPGIVGQKQCGTFLHPVLVVDA
SSHIPIGFSSVKQWNRSPAALSREERNYRYQPIEEKESYRWIESGMAASEQMPRDAVKTIIGDREADIFELFSRIPTDNV
HLLIRSVHERNCRLDDPDCSVHLNTLMEQAVLRAEYSFEVLPGSGRKKRVACMELRFERVTLCAPVNGPAKGSPPVSLYC
IHVKEKSSSTPVNESPIEWRLLTTHVVETVEQAIECIGWYRCRWLIEELFRVLKRKGFMIEDAQLETVSALQKLILISLQ
AALQVMVLKLSFDKEDEKLSSEIYFTSKEIALLHIVGKKSEGNTKIQQNPYKKESMAWAAWIIARLGAWSAYKSQSIPGY
ITFKNGLDRFYTQFELYELIS

Sequences:

>Translated_421_residues
MGAYRFLNNSSVSSDAILSGLIHTCCKNASGRQHLLCIQDTSEINYEAHVERMKKKTASPGIVGQKQCGTFLHPVLVVDA
SSHIPIGFSSVKQWNRSPAALSREERNYRYQPIEEKESYRWIESGMAASEQMPRDAVKTIIGDREADIFELFSRIPTDNV
HLLIRSVHERNCRLDDPDCSVHLNTLMEQAVLRAEYSFEVLPGSGRKKRVACMELRFERVTLCAPVNGPAKGSPPVSLYC
IHVKEKSSSTPVNESPIEWRLLTTHVVETVEQAIECIGWYRCRWLIEELFRVLKRKGFMIEDAQLETVSALQKLILISLQ
AALQVMVLKLSFDKEDEKLSSEIYFTSKEIALLHIVGKKSEGNTKIQQNPYKKESMAWAAWIIARLGAWSAYKSQSIPGY
ITFKNGLDRFYTQFELYELIS
>Mature_420_residues
GAYRFLNNSSVSSDAILSGLIHTCCKNASGRQHLLCIQDTSEINYEAHVERMKKKTASPGIVGQKQCGTFLHPVLVVDAS
SHIPIGFSSVKQWNRSPAALSREERNYRYQPIEEKESYRWIESGMAASEQMPRDAVKTIIGDREADIFELFSRIPTDNVH
LLIRSVHERNCRLDDPDCSVHLNTLMEQAVLRAEYSFEVLPGSGRKKRVACMELRFERVTLCAPVNGPAKGSPPVSLYCI
HVKEKSSSTPVNESPIEWRLLTTHVVETVEQAIECIGWYRCRWLIEELFRVLKRKGFMIEDAQLETVSALQKLILISLQA
ALQVMVLKLSFDKEDEKLSSEIYFTSKEIALLHIVGKKSEGNTKIQQNPYKKESMAWAAWIIARLGAWSAYKSQSIPGYI
TFKNGLDRFYTQFELYELIS

Specific function: Mediates transposition of transposon Tn5 by a 'cut and paste' mechanism. First, the monomeric transposase binds the 19 bp inverted DNA repeats flanking the transposon. Then, dimerization of the DNA-bound transposase creates a synaptic DNA complex. After n

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002559
- InterPro:   IPR003201
- InterPro:   IPR014737
- InterPro:   IPR014736
- InterPro:   IPR014735 [H]

Pfam domain/function: PF01609 Transposase_11; PF02281 Transposase_Tn5 [H]

EC number: NA

Molecular weight: Translated: 47887; Mature: 47755

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAYRFLNNSSVSSDAILSGLIHTCCKNASGRQHLLCIQDTSEINYEAHVERMKKKTASP
CCCCEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCC
GIVGQKQCGTFLHPVLVVDASSHIPIGFSSVKQWNRSPAALSREERNYRYQPIEEKESYR
CCCCHHHCCHHHCEEEEEECCCCCCCCHHHHHHCCCCCHHHCHHHCCCCCCCCCHHHHHH
WIESGMAASEQMPRDAVKTIIGDREADIFELFSRIPTDNVHLLIRSVHERNCRLDDPDCS
HHHHCCHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCC
VHLNTLMEQAVLRAEYSFEVLPGSGRKKRVACMELRFERVTLCAPVNGPAKGSPPVSLYC
EEHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCEEEEEECCCCCCCCCCCEEEEE
IHVKEKSSSTPVNESPIEWRLLTTHVVETVEQAIECIGWYRCRWLIEELFRVLKRKGFMI
EEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
EDAQLETVSALQKLILISLQAALQVMVLKLSFDKEDEKLSSEIYFTSKEIALLHIVGKKS
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEEECCCEEEEEEEECCC
EGNTKIQQNPYKKESMAWAAWIIARLGAWSAYKSQSIPGYITFKNGLDRFYTQFELYELI
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEECCCHHHHHHHHHHHHHC
S
C
>Mature Secondary Structure 
GAYRFLNNSSVSSDAILSGLIHTCCKNASGRQHLLCIQDTSEINYEAHVERMKKKTASP
CCCEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCC
GIVGQKQCGTFLHPVLVVDASSHIPIGFSSVKQWNRSPAALSREERNYRYQPIEEKESYR
CCCCHHHCCHHHCEEEEEECCCCCCCCHHHHHHCCCCCHHHCHHHCCCCCCCCCHHHHHH
WIESGMAASEQMPRDAVKTIIGDREADIFELFSRIPTDNVHLLIRSVHERNCRLDDPDCS
HHHHCCHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCC
VHLNTLMEQAVLRAEYSFEVLPGSGRKKRVACMELRFERVTLCAPVNGPAKGSPPVSLYC
EEHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCEEEEEECCCCCCCCCCCEEEEE
IHVKEKSSSTPVNESPIEWRLLTTHVVETVEQAIECIGWYRCRWLIEELFRVLKRKGFMI
EEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
EDAQLETVSALQKLILISLQAALQVMVLKLSFDKEDEKLSSEIYFTSKEIALLHIVGKKS
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEEECCCEEEEEEEECCC
EGNTKIQQNPYKKESMAWAAWIIARLGAWSAYKSQSIPGYITFKNGLDRFYTQFELYELI
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCEEEEECCCHHHHHHHHHHHHHC
S
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6260374; 6271452; 7867940; 6291786; 10884228; 12367522; 11896402 [H]