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The map label for this gene is murI [H]

Identifier: 29349130

GI number: 29349130

Start: 4831721

End: 4832563

Strand: Reverse

Name: murI [H]

Synonym: BT_3722

Alternate gene names: 29349130

Gene position: 4832563-4831721 (Counterclockwise)

Preceding gene: 29349131

Following gene: 29349129

Centisome position: 77.19

GC content: 46.5

Gene sequence:

>843_bases
ATGAAGCAACATCTATCTCATACACCCGGACCAATCGGAGTTTTCGATTCCGGATACGGTGGGCTGACTATCCTCAACAA
GATAAGAGAGGCACTGCCGGAATATGATTATGTCTATCTGGGAGACAATGCACGCACTCCTTACGGCACACGTTCTTTTG
AAATCGTGTACGAATTCACTTTGCAGGCAGTCAATAAATTGTTTGAAATGGGATGTCATCTCGTTATCTTGGCCTGCAAT
ACAGCCTCTGCCAAAGCATTGCGCAGCATACAAATGAATGACTTGCCCGGCATTGACCCTGAACGCCGTGTACTCGGAGT
TATCCGCCCTACCGTTGAGTGTATAGGTGGCATTACTCAAAGCCGTCATATCGGAGTACTGGCAACAGCAGGTACCATCA
AGTCCGAATCGTATCCATTAGAGATACATAAGCTATTCCCGGATATTCAAGTGAGCGGAATGGCATGCCCCATGTGGGTA
CCGCTAGTGGAAAATAATGAATCACAGAACGAAGGGGCCGATTATTTCATCCGGAAATACATTGACCAGCTGCTATCCAA
AGATCCCGAAATTGATACGATGATTCTGGGCTGCACGCATTATCCTATCTTGCTCCCTAAGATACAAGAATACACTCCGA
AGCATATCCGCATTGTCGCACAAGGAGAATATGTAGCCGAAAGTCTGAAGGACTACCTAAGCAGACACCCGGAAATGAAT
GCCAAGTGTACCCAAAACGGCAGCTGTCTGTTTTACACCACAGAGGCGGAAGAGAAGTTTGTCGAATCGGCCTCTTCCTT
CCTGAACCAGCAAATCGACGTAAGAAGAATTTCTCTCGAATAA

Upstream 100 bases:

>100_bases
ATAAGCTATAAAATACCGAAAGGGAAAGAGAAAAGAAGATCATACATCTTTCTCTTTCCCTTTTTTCATGTAACTTTGCG
TATATAAAACCGCACTCGTC

Downstream 100 bases:

>100_bases
ATATGGGAAAGCTGATCAATCTTTCCTTCTTTTTTGTTATATTTGTATACATAACCTCAAATAGCATAAGATTATGAAAG
AAGAAGATTATAGCAAGGCC

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MKQHLSHTPGPIGVFDSGYGGLTILNKIREALPEYDYVYLGDNARTPYGTRSFEIVYEFTLQAVNKLFEMGCHLVILACN
TASAKALRSIQMNDLPGIDPERRVLGVIRPTVECIGGITQSRHIGVLATAGTIKSESYPLEIHKLFPDIQVSGMACPMWV
PLVENNESQNEGADYFIRKYIDQLLSKDPEIDTMILGCTHYPILLPKIQEYTPKHIRIVAQGEYVAESLKDYLSRHPEMN
AKCTQNGSCLFYTTEAEEKFVESASSFLNQQIDVRRISLE

Sequences:

>Translated_280_residues
MKQHLSHTPGPIGVFDSGYGGLTILNKIREALPEYDYVYLGDNARTPYGTRSFEIVYEFTLQAVNKLFEMGCHLVILACN
TASAKALRSIQMNDLPGIDPERRVLGVIRPTVECIGGITQSRHIGVLATAGTIKSESYPLEIHKLFPDIQVSGMACPMWV
PLVENNESQNEGADYFIRKYIDQLLSKDPEIDTMILGCTHYPILLPKIQEYTPKHIRIVAQGEYVAESLKDYLSRHPEMN
AKCTQNGSCLFYTTEAEEKFVESASSFLNQQIDVRRISLE
>Mature_280_residues
MKQHLSHTPGPIGVFDSGYGGLTILNKIREALPEYDYVYLGDNARTPYGTRSFEIVYEFTLQAVNKLFEMGCHLVILACN
TASAKALRSIQMNDLPGIDPERRVLGVIRPTVECIGGITQSRHIGVLATAGTIKSESYPLEIHKLFPDIQVSGMACPMWV
PLVENNESQNEGADYFIRKYIDQLLSKDPEIDTMILGCTHYPILLPKIQEYTPKHIRIVAQGEYVAESLKDYLSRHPEMN
AKCTQNGSCLFYTTEAEEKFVESASSFLNQQIDVRRISLE

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis [H]

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family [H]

Homologues:

Organism=Escherichia coli, GI87082355, Length=251, Percent_Identity=31.8725099601594, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391 [H]

Pfam domain/function: PF01177 Asp_Glu_race [H]

EC number: =5.1.1.3 [H]

Molecular weight: Translated: 31466; Mature: 31466

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00923 ASP_GLU_RACEMASE_1 ; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQHLSHTPGPIGVFDSGYGGLTILNKIREALPEYDYVYLGDNARTPYGTRSFEIVYEFT
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEEHHHHHH
LQAVNKLFEMGCHLVILACNTASAKALRSIQMNDLPGIDPERRVLGVIRPTVECIGGITQ
HHHHHHHHHCCCEEEEEEECCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
SRHIGVLATAGTIKSESYPLEIHKLFPDIQVSGMACPMWVPLVENNESQNEGADYFIRKY
CCCEEEEEECCCCCCCCCCEEHHHHCCCCEECCEECCEEEEEECCCCCCCCCHHHHHHHH
IDQLLSKDPEIDTMILGCTHYPILLPKIQEYTPKHIRIVAQGEYVAESLKDYLSRHPEMN
HHHHHCCCCCCCEEEEECCCCCEECCCHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCC
AKCTQNGSCLFYTTEAEEKFVESASSFLNQQIDVRRISLE
CEECCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEEECC
>Mature Secondary Structure
MKQHLSHTPGPIGVFDSGYGGLTILNKIREALPEYDYVYLGDNARTPYGTRSFEIVYEFT
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCEEHHHHHH
LQAVNKLFEMGCHLVILACNTASAKALRSIQMNDLPGIDPERRVLGVIRPTVECIGGITQ
HHHHHHHHHCCCEEEEEEECCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
SRHIGVLATAGTIKSESYPLEIHKLFPDIQVSGMACPMWVPLVENNESQNEGADYFIRKY
CCCEEEEEECCCCCCCCCCEEHHHHCCCCEECCEECCEEEEEECCCCCCCCCHHHHHHHH
IDQLLSKDPEIDTMILGCTHYPILLPKIQEYTPKHIRIVAQGEYVAESLKDYLSRHPEMN
HHHHHCCCCCCCEEEEECCCCCEECCCHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCC
AKCTQNGSCLFYTTEAEEKFVESASSFLNQQIDVRRISLE
CEECCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA