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The map label for this gene is dapE [H]

Identifier: 29348958

GI number: 29348958

Start: 4588590

End: 4589657

Strand: Reverse

Name: dapE [H]

Synonym: BT_3549

Alternate gene names: 29348958

Gene position: 4589657-4588590 (Counterclockwise)

Preceding gene: 29348960

Following gene: 29348949

Centisome position: 73.31

GC content: 46.72

Gene sequence:

>1068_bases
ATGAAATACGATATTCCAACCATGACAGCCGAAGCTGTAAGCCTGTTGAAATCACTAATCAGCATTCCGTCCATCAGTCG
GGAAGAGACGCAAGCCGCTGATTTCCTGCAAAATTATATCGAAGCCGAAGGGATGCAGACCGGACGCAAGGGAAACAATG
TTTGGTGCCTCAGTCCTATGTTCGACCTTAAAAAGCCGACAATCCTGCTCAATTCTCATATCGACACGGTAAAACCCGTA
AATGGCTGGCGCAAAGACCCTTTCACACCCCGCGAGGAAAACGGTAAATTATACGGATTGGGCAGTAACGACGCCGGAGC
CAGTGTTGTTTCGCTCTTACAGGTGTTCCTGCAACTATGTCGCACTTCTCAAAATTACAATCTCATCTATCTGGCTTCCT
GCGAAGAAGAAGTTTCGGGTAAGGAGGGAATTGAAAGTGTATTACCGGGGTTACCCCCTGTTTCATTCGCTATCGTAGGC
GAACCTACGGAGATGCAACCTGCCATTGCCGAAAAGGGGTTGATGGTACTGGATGTAACTGCTACGGGAAAAGCCGGACA
TGCCGCACGCGATGAAGGAGATAATGCCATTTATAAGGTATTGAACGATATCGCCTGGTTCCGTGATTATCGTTTCGAGA
AAGAATCACCTTTACTCGGACCGGTAAAAATGAGCGTTACGGTCATCAATGCAGGCACTCAGCACAATGTAGTTCCCGAT
AAATGTACATTTGTTGTTGATATCCGAAGCAACGAACTTTACTCCAACGAAGATTTATTTGCAGAGATCAGAAAGCATAT
AGCCTGCGATGCGAAAGCCCGCTCGTTCCGCCTCAACTCTTCACGAATTGATGAGAAACATCCGTTCGTACAGAAAGCGG
TGAAGATGGGACGCATACCTTTCGGTTCCCCGACTTTGTCAGATCAGGCATTGATGTCCTTTGCGTCGGTAAAGATCGGT
CCGGGACGCTCTTCACGTTCACATACAGCCGAAGAGTATATTATGCTGAAAGAGATAGAAGAGGCTATCGGGATTTATTT
AGATTTGTTAGACGGACTAAAGCTTTAG

Upstream 100 bases:

>100_bases
ATTGATAAATATGGTACAAAGGTATTAGTTTTATTTGAAACTATTGCGCAAAACGAGTATCTTTGTGCAAATATTAAGCT
CTAAATTAACAAGTTTGTAT

Downstream 100 bases:

>100_bases
TCCGTCTTTACCACTCATCAAGTAGAAAATCGGCTATATGCCGATGCTTAACCCCTTCAATATTATCTTGCCATAGATCA
TCCATACTAACCACATATTT

Product: acetylornithine deacetylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKYDIPTMTAEAVSLLKSLISIPSISREETQAADFLQNYIEAEGMQTGRKGNNVWCLSPMFDLKKPTILLNSHIDTVKPV
NGWRKDPFTPREENGKLYGLGSNDAGASVVSLLQVFLQLCRTSQNYNLIYLASCEEEVSGKEGIESVLPGLPPVSFAIVG
EPTEMQPAIAEKGLMVLDVTATGKAGHAARDEGDNAIYKVLNDIAWFRDYRFEKESPLLGPVKMSVTVINAGTQHNVVPD
KCTFVVDIRSNELYSNEDLFAEIRKHIACDAKARSFRLNSSRIDEKHPFVQKAVKMGRIPFGSPTLSDQALMSFASVKIG
PGRSSRSHTAEEYIMLKEIEEAIGIYLDLLDGLKL

Sequences:

>Translated_355_residues
MKYDIPTMTAEAVSLLKSLISIPSISREETQAADFLQNYIEAEGMQTGRKGNNVWCLSPMFDLKKPTILLNSHIDTVKPV
NGWRKDPFTPREENGKLYGLGSNDAGASVVSLLQVFLQLCRTSQNYNLIYLASCEEEVSGKEGIESVLPGLPPVSFAIVG
EPTEMQPAIAEKGLMVLDVTATGKAGHAARDEGDNAIYKVLNDIAWFRDYRFEKESPLLGPVKMSVTVINAGTQHNVVPD
KCTFVVDIRSNELYSNEDLFAEIRKHIACDAKARSFRLNSSRIDEKHPFVQKAVKMGRIPFGSPTLSDQALMSFASVKIG
PGRSSRSHTAEEYIMLKEIEEAIGIYLDLLDGLKL
>Mature_355_residues
MKYDIPTMTAEAVSLLKSLISIPSISREETQAADFLQNYIEAEGMQTGRKGNNVWCLSPMFDLKKPTILLNSHIDTVKPV
NGWRKDPFTPREENGKLYGLGSNDAGASVVSLLQVFLQLCRTSQNYNLIYLASCEEEVSGKEGIESVLPGLPPVSFAIVG
EPTEMQPAIAEKGLMVLDVTATGKAGHAARDEGDNAIYKVLNDIAWFRDYRFEKESPLLGPVKMSVTVINAGTQHNVVPD
KCTFVVDIRSNELYSNEDLFAEIRKHIACDAKARSFRLNSSRIDEKHPFVQKAVKMGRIPFGSPTLSDQALMSFASVKIG
PGRSSRSHTAEEYIMLKEIEEAIGIYLDLLDGLKL

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790395, Length=376, Percent_Identity=25, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788816, Length=275, Percent_Identity=21.4545454545455, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17551016, Length=205, Percent_Identity=28.2926829268293, Blast_Score=68, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 39218; Mature: 39218

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYDIPTMTAEAVSLLKSLISIPSISREETQAADFLQNYIEAEGMQTGRKGNNVWCLSPM
CCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCH
FDLKKPTILLNSHIDTVKPVNGWRKDPFTPREENGKLYGLGSNDAGASVVSLLQVFLQLC
HCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
RTSQNYNLIYLASCEEEVSGKEGIESVLPGLPPVSFAIVGEPTEMQPAIAEKGLMVLDVT
HCCCCCCEEEEECCCHHCCCCCCHHHHCCCCCCCEEEEECCCCCCCHHHHHCCEEEEEEE
ATGKAGHAARDEGDNAIYKVLNDIAWFRDYRFEKESPLLGPVKMSVTVINAGTQHNVVPD
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCCC
KCTFVVDIRSNELYSNEDLFAEIRKHIACDAKARSFRLNSSRIDEKHPFVQKAVKMGRIP
CEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHEEECCCHHCCCCCHHHHHHHHHCCCC
FGSPTLSDQALMSFASVKIGPGRSSRSHTAEEYIMLKEIEEAIGIYLDLLDGLKL
CCCCCCCHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKYDIPTMTAEAVSLLKSLISIPSISREETQAADFLQNYIEAEGMQTGRKGNNVWCLSPM
CCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCH
FDLKKPTILLNSHIDTVKPVNGWRKDPFTPREENGKLYGLGSNDAGASVVSLLQVFLQLC
HCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
RTSQNYNLIYLASCEEEVSGKEGIESVLPGLPPVSFAIVGEPTEMQPAIAEKGLMVLDVT
HCCCCCCEEEEECCCHHCCCCCCHHHHCCCCCCCEEEEECCCCCCCHHHHHCCEEEEEEE
ATGKAGHAARDEGDNAIYKVLNDIAWFRDYRFEKESPLLGPVKMSVTVINAGTQHNVVPD
ECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCCCCCC
KCTFVVDIRSNELYSNEDLFAEIRKHIACDAKARSFRLNSSRIDEKHPFVQKAVKMGRIP
CEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHEEECCCHHCCCCCHHHHHHHHHCCCC
FGSPTLSDQALMSFASVKIGPGRSSRSHTAEEYIMLKEIEEAIGIYLDLLDGLKL
CCCCCCCHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA