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The map label for this gene is lacZ [H]

Identifier: 29348378

GI number: 29348378

Start: 3743935

End: 3746043

Strand: Reverse

Name: lacZ [H]

Synonym: BT_2969

Alternate gene names: 29348378

Gene position: 3746043-3743935 (Counterclockwise)

Preceding gene: 29348379

Following gene: 29348377

Centisome position: 59.84

GC content: 44.95

Gene sequence:

>2109_bases
ATGAAGTTTAAATATATACTCATTTTATCAGCGCTTATCGGGCAGATTCTTTCACTCTCTGCCCGGGAAGTGACCTCTTT
CAATGAAGGATGGCTATTCAAACGCGGGCCTTTTTCACAGGACCCGGTGAAGGTCGCTGCTCAATGGAATGCGGACTGGG
AAACTGTAAACTTGCCTCATACCTGGAACGCTAAAGATATGCAGGTAAAAGCTGATGCTTTCTATGAAGGAGTTGGTTAT
TACAGGAAAACACAATTCTTTGGCAATGAGTTGAAAGGGAAGCGTGTATTTCTTCGCTTTGAAGGTGTGGGCGCCAACAC
AGAAGTGTATGTCAACGGTAAACTTGTCGGAATGCACCGGGGAGCATACAGCGCCTTTGCTTTTGAAATTGGAACCGCAT
TGAAACTGGGAGCTGAGAATGAGATAATGGTAAAGGCGGATAATGCTTCACGTTCTGATGTGATTCCGGTAAATCACAAT
CTTTTTGGGGTTTACGGAGGTATCTATCGTCCGGTATGGCTGATTATTACTGAACAGAATAACATTACAGTTACCGATTG
TGCCTCTCCGGGTGTATATATTACTCAAAGGAATGTATCGAAACGCTCTGCCGATATCACAGTGAAAGTGAAGCTGGACA
ATGGAAGTCTTACTCCTGCTGACTTGGTACTCGAAAATACCATTTATACGCAGAATGGCAAGAAGGTCACTTCCCATCGC
CTTCCTTTGGTACTCACTCCGCAGGGAACACAGACTTACATTTCTACCTTCAAACTGAATAAACCGCATCTGTGGCAGGG
ACGTAAGGACCCTTATTTATATAAGGTTATTTCCCGCTTACTGGCGGACGGAAAAGTAATTGATGAGGTGGTACAGCCAT
TGGGTGTTCGTAGATATGAGATTGTGCCAGGCAAAGGTTTCTACTTGAATGGTGAGAAATACCCGATGTATGGGGTAACC
CGTCATCAGGATTGGTGGGGATTGGGTAGTGCGCTTACCAACAAAGAACATGATTTCGACTTGGCTCAGATTATGGATAT
CGGAGCGACTACTGTCCGTTTCGCCCACTATCAACAGTCGGATTACCTGTATTCCCGTTGCGATTCTTTGGGATTGGTCA
TCTGGGCAGAGATTCCTTTTGTGAATCGGGTGACAGGATATGAAGCCGAAAATGCACAGTCTCAGCTTCGTGAACTGATT
CGTCAAAGTTTCAATCACCCTTCCATTTATGTATGGGGATTGCACAATGAAGTATACCAGCCCCACGAATATACGGCAGG
ACTGACACAGACTCTGCATAATCTGGCCAAGACAGAGGATCCTGACCGCTACACTGTATCTGTAAATGGTTATGGCCATG
CAGAGCATCCGGTCAATCAAAATGCGGATATTCAGGGAATGAACCGCTACTTCGGATGGTATGAGAAAAAGTTGCAGGAT
ATCGAGCCTTGGGTAAAAGGGCTGGAGGAGAAATACCCGTGGCAGAAACTGATGTTGACGGAATATGGTGCTGATGCAAA
TCTGGACCATCAGACAGAATATCTGGGTGATGCTCTGAACTGGGGAAAGTCTTTCTATCCGGAAACTTTTCAGACCAAGA
CGCATGAATACCAATGGAGTGTCATTGCAAAGCATCCCTATATCATCGCTTCTTATCTTTGGAATATGTTTGACTTTGGT
GTACCGATGTGGAGCCGTGGCGGTGTACCTGCCCGTAACTTGAAGGGGTTGATCACTTTTGACCGGAAAATCAAAAAGGA
CTCCTATTACTGGTATAAAGCTAACTGGAGCAAAGATCCCGTACTCTACCTGACCCAGCGTCGCAACATCGACCGCGAAC
GAAAACATACTTCTGTCACTGTTTATTCTAACATAGGTACACCGCAAGTGTATCTTAATGGAAAAGAACTGACGGGTATA
CGCCAAGGCTACACGGATGTGCACTATGTCTTGGCTGATGTAACCTTGGAACAAGGTAAGAATACAATTAAAACCGTTGC
AACATATAATGGAAAAGAATACACTGACGAGATTGAATGGAACTATACCGGCGAAAAGAAGCGAAGTGCCGATTGGAGTG
AAAACAAAGAAGAGCACGCAGGTTGGTAA

Upstream 100 bases:

>100_bases
CCGGGTGAACCTTATGTAATTCAGCTTAAAGTTCGTGCAGCTAAAGGAACAAAAAGTATTTATCGAGATGCTTTAACATA
AATGATAAAGAAACGATTCT

Downstream 100 bases:

>100_bases
TCGGAGACGTTTCTGTATGTAATCTGAAAAATGTTGAATCTAAAATATAAAAGCCTATGAATGAAATGAGAAAGAATCTC
TTTTAAATGGAAAACAAGTA

Product: beta-galactosidase

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 702; Mature: 702

Protein sequence:

>702_residues
MKFKYILILSALIGQILSLSAREVTSFNEGWLFKRGPFSQDPVKVAAQWNADWETVNLPHTWNAKDMQVKADAFYEGVGY
YRKTQFFGNELKGKRVFLRFEGVGANTEVYVNGKLVGMHRGAYSAFAFEIGTALKLGAENEIMVKADNASRSDVIPVNHN
LFGVYGGIYRPVWLIITEQNNITVTDCASPGVYITQRNVSKRSADITVKVKLDNGSLTPADLVLENTIYTQNGKKVTSHR
LPLVLTPQGTQTYISTFKLNKPHLWQGRKDPYLYKVISRLLADGKVIDEVVQPLGVRRYEIVPGKGFYLNGEKYPMYGVT
RHQDWWGLGSALTNKEHDFDLAQIMDIGATTVRFAHYQQSDYLYSRCDSLGLVIWAEIPFVNRVTGYEAENAQSQLRELI
RQSFNHPSIYVWGLHNEVYQPHEYTAGLTQTLHNLAKTEDPDRYTVSVNGYGHAEHPVNQNADIQGMNRYFGWYEKKLQD
IEPWVKGLEEKYPWQKLMLTEYGADANLDHQTEYLGDALNWGKSFYPETFQTKTHEYQWSVIAKHPYIIASYLWNMFDFG
VPMWSRGGVPARNLKGLITFDRKIKKDSYYWYKANWSKDPVLYLTQRRNIDRERKHTSVTVYSNIGTPQVYLNGKELTGI
RQGYTDVHYVLADVTLEQGKNTIKTVATYNGKEYTDEIEWNYTGEKKRSADWSENKEEHAGW

Sequences:

>Translated_702_residues
MKFKYILILSALIGQILSLSAREVTSFNEGWLFKRGPFSQDPVKVAAQWNADWETVNLPHTWNAKDMQVKADAFYEGVGY
YRKTQFFGNELKGKRVFLRFEGVGANTEVYVNGKLVGMHRGAYSAFAFEIGTALKLGAENEIMVKADNASRSDVIPVNHN
LFGVYGGIYRPVWLIITEQNNITVTDCASPGVYITQRNVSKRSADITVKVKLDNGSLTPADLVLENTIYTQNGKKVTSHR
LPLVLTPQGTQTYISTFKLNKPHLWQGRKDPYLYKVISRLLADGKVIDEVVQPLGVRRYEIVPGKGFYLNGEKYPMYGVT
RHQDWWGLGSALTNKEHDFDLAQIMDIGATTVRFAHYQQSDYLYSRCDSLGLVIWAEIPFVNRVTGYEAENAQSQLRELI
RQSFNHPSIYVWGLHNEVYQPHEYTAGLTQTLHNLAKTEDPDRYTVSVNGYGHAEHPVNQNADIQGMNRYFGWYEKKLQD
IEPWVKGLEEKYPWQKLMLTEYGADANLDHQTEYLGDALNWGKSFYPETFQTKTHEYQWSVIAKHPYIIASYLWNMFDFG
VPMWSRGGVPARNLKGLITFDRKIKKDSYYWYKANWSKDPVLYLTQRRNIDRERKHTSVTVYSNIGTPQVYLNGKELTGI
RQGYTDVHYVLADVTLEQGKNTIKTVATYNGKEYTDEIEWNYTGEKKRSADWSENKEEHAGW
>Mature_702_residues
MKFKYILILSALIGQILSLSAREVTSFNEGWLFKRGPFSQDPVKVAAQWNADWETVNLPHTWNAKDMQVKADAFYEGVGY
YRKTQFFGNELKGKRVFLRFEGVGANTEVYVNGKLVGMHRGAYSAFAFEIGTALKLGAENEIMVKADNASRSDVIPVNHN
LFGVYGGIYRPVWLIITEQNNITVTDCASPGVYITQRNVSKRSADITVKVKLDNGSLTPADLVLENTIYTQNGKKVTSHR
LPLVLTPQGTQTYISTFKLNKPHLWQGRKDPYLYKVISRLLADGKVIDEVVQPLGVRRYEIVPGKGFYLNGEKYPMYGVT
RHQDWWGLGSALTNKEHDFDLAQIMDIGATTVRFAHYQQSDYLYSRCDSLGLVIWAEIPFVNRVTGYEAENAQSQLRELI
RQSFNHPSIYVWGLHNEVYQPHEYTAGLTQTLHNLAKTEDPDRYTVSVNGYGHAEHPVNQNADIQGMNRYFGWYEKKLQD
IEPWVKGLEEKYPWQKLMLTEYGADANLDHQTEYLGDALNWGKSFYPETFQTKTHEYQWSVIAKHPYIIASYLWNMFDFG
VPMWSRGGVPARNLKGLITFDRKIKKDSYYWYKANWSKDPVLYLTQRRNIDRERKHTSVTVYSNIGTPQVYLNGKELTGI
RQGYTDVHYVLADVTLEQGKNTIKTVATYNGKEYTDEIEWNYTGEKKRSADWSENKEEHAGW

Specific function: Unknown

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=572, Percent_Identity=23.2517482517483, Blast_Score=95, Evalue=3e-19,
Organism=Escherichia coli, GI1787903, Length=644, Percent_Identity=25.4658385093168, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI48994920, Length=409, Percent_Identity=25.9168704156479, Blast_Score=113, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17510825, Length=625, Percent_Identity=24.64, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24655438, Length=613, Percent_Identity=25.2854812398042, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19922582, Length=613, Percent_Identity=25.2854812398042, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI62471735, Length=613, Percent_Identity=25.2854812398042, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24651745, Length=618, Percent_Identity=23.9482200647249, Blast_Score=102, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 80731; Mature: 80731

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKYILILSALIGQILSLSAREVTSFNEGWLFKRGPFSQDPVKVAAQWNADWETVNLPH
CCEEHHHHHHHHHHHHHCCCHHHHCCCCCCCEEECCCCCCCCEEEEEEECCCCEEEECCC
TWNAKDMQVKADAFYEGVGYYRKTQFFGNELKGKRVFLRFEGVGANTEVYVNGKLVGMHR
CCCCCCEEEEHHHHHHCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEECCEEEEEEC
GAYSAFAFEIGTALKLGAENEIMVKADNASRSDVIPVNHNLFGVYGGIYRPVWLIITEQN
CCCHHHHEECCCEEEECCCCEEEEEECCCCCCCEEEECCCEEEECCCCCCEEEEEEECCC
NITVTDCASPGVYITQRNVSKRSADITVKVKLDNGSLTPADLVLENTIYTQNGKKVTSHR
CEEEEECCCCCEEEEECCCCCCCCEEEEEEEECCCCCCHHHEEEECEEEECCCCEECCCC
LPLVLTPQGTQTYISTFKLNKPHLWQGRKDPYLYKVISRLLADGKVIDEVVQPLGVRRYE
CCEEECCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCEEEE
IVPGKGFYLNGEKYPMYGVTRHQDWWGLGSALTNKEHDFDLAQIMDIGATTVRFAHYQQS
EECCCCEEECCCCCCEECCCCCCCCCCCCHHHCCCCCCCCHHHHHHCCCCEEEEEEECCC
DYLYSRCDSLGLVIWAEIPFVNRVTGYEAENAQSQLRELIRQSFNHPSIYVWGLHNEVYQ
CHHHHHHCCCCEEEEEECCCEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCC
PHEYTAGLTQTLHNLAKTEDPDRYTVSVNGYGHAEHPVNQNADIQGMNRYFGWYEKKLQD
CCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
IEPWVKGLEEKYPWQKLMLTEYGADANLDHQTEYLGDALNWGKSFYPETFQTKTHEYQWS
HHHHHHHHHHHCCHHHEEEEECCCCCCCCCHHHHHHHHHHCCHHCCCCCCCCCCCEEEEE
VIAKHPYIIASYLWNMFDFGVPMWSRGGVPARNLKGLITFDRKIKKDSYYWYKANWSKDP
EEECCCCHHHHHHHHHHHCCCCCCCCCCCCHHHCCEEEEECCEECCCCEEEEEECCCCCC
VLYLTQRRNIDRERKHTSVTVYSNIGTPQVYLNGKELTGIRQGYTDVHYVLADVTLEQGK
EEEEEECCCCCCHHCCCEEEEEECCCCCEEEECCCEECHHHCCCCCEEEEEEEEEHHCCC
NTIKTVATYNGKEYTDEIEWNYTGEKKRSADWSENKEEHAGW
CEEEEEEEECCCEECCEEEEEECCCCCCCCCCCCCCHHCCCC
>Mature Secondary Structure
MKFKYILILSALIGQILSLSAREVTSFNEGWLFKRGPFSQDPVKVAAQWNADWETVNLPH
CCEEHHHHHHHHHHHHHCCCHHHHCCCCCCCEEECCCCCCCCEEEEEEECCCCEEEECCC
TWNAKDMQVKADAFYEGVGYYRKTQFFGNELKGKRVFLRFEGVGANTEVYVNGKLVGMHR
CCCCCCEEEEHHHHHHCCCHHHHHHHHCCCCCCCEEEEEEECCCCCCEEEECCEEEEEEC
GAYSAFAFEIGTALKLGAENEIMVKADNASRSDVIPVNHNLFGVYGGIYRPVWLIITEQN
CCCHHHHEECCCEEEECCCCEEEEEECCCCCCCEEEECCCEEEECCCCCCEEEEEEECCC
NITVTDCASPGVYITQRNVSKRSADITVKVKLDNGSLTPADLVLENTIYTQNGKKVTSHR
CEEEEECCCCCEEEEECCCCCCCCEEEEEEEECCCCCCHHHEEEECEEEECCCCEECCCC
LPLVLTPQGTQTYISTFKLNKPHLWQGRKDPYLYKVISRLLADGKVIDEVVQPLGVRRYE
CCEEECCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCEEEE
IVPGKGFYLNGEKYPMYGVTRHQDWWGLGSALTNKEHDFDLAQIMDIGATTVRFAHYQQS
EECCCCEEECCCCCCEECCCCCCCCCCCCHHHCCCCCCCCHHHHHHCCCCEEEEEEECCC
DYLYSRCDSLGLVIWAEIPFVNRVTGYEAENAQSQLRELIRQSFNHPSIYVWGLHNEVYQ
CHHHHHHCCCCEEEEEECCCEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCC
PHEYTAGLTQTLHNLAKTEDPDRYTVSVNGYGHAEHPVNQNADIQGMNRYFGWYEKKLQD
CCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
IEPWVKGLEEKYPWQKLMLTEYGADANLDHQTEYLGDALNWGKSFYPETFQTKTHEYQWS
HHHHHHHHHHHCCHHHEEEEECCCCCCCCCHHHHHHHHHHCCHHCCCCCCCCCCCEEEEE
VIAKHPYIIASYLWNMFDFGVPMWSRGGVPARNLKGLITFDRKIKKDSYYWYKANWSKDP
EEECCCCHHHHHHHHHHHCCCCCCCCCCCCHHHCCEEEEECCEECCCCEEEEEECCCCCC
VLYLTQRRNIDRERKHTSVTVYSNIGTPQVYLNGKELTGIRQGYTDVHYVLADVTLEQGK
EEEEEECCCCCCHHCCCEEEEEECCCCCEEEECCCEECHHHCCCCCEEEEEEEEEHHCCC
NTIKTVATYNGKEYTDEIEWNYTGEKKRSADWSENKEEHAGW
CEEEEEEEECCCEECCEEEEEECCCCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA