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The map label for this gene is epsD [H]

Identifier: 29348354

GI number: 29348354

Start: 3707252

End: 3708454

Strand: Reverse

Name: epsD [H]

Synonym: BT_2945

Alternate gene names: 29348354

Gene position: 3708454-3707252 (Counterclockwise)

Preceding gene: 29348357

Following gene: 29348353

Centisome position: 59.24

GC content: 51.04

Gene sequence:

>1203_bases
ATGAAAAAGGTTGTGTTTTTAGGATTAGGGTATATCGGCCTTCCTACGGCAGCCGTTGCTGCCGCTCACGGTTTCGAAGT
CATAGGAGTAGATGTCAATCCTTCTGTGGTGGAAACGATCAACCAGGGAAAGATACATATTGTAGAACCTGAACTGGGTC
AGGTAGTCAGGGACGTAGTGCAGAGTGGAAAGTTACGCGCTGTATGCAAGCCGGAAGCGGCGGATGCTTTCTTTATCGTA
GTGCCTACCCCATTCAAGCAGAACCATCGGGCGGACATCACCTATGTGGAAGCGGCCACCCGTTCCGTGATCCCTTATCT
GCAGGAGGGTAACCTGTTTGTCATTGAATCCACTTCTCCGGTATATACCACCGAACGTATGGCGGAAGTGATCTATAAAG
AACGTCCCGAACTGAAAGGGAAGATACATATCGCTTATTGTCCCGAACGCGTATTGCCGGGAAATACATTATACGAACTG
GTGCACAACGACCGCGTGATAGGCGGCATCGATCCGGCATCCACCGCAAAAGCCATTGAGTTTTATTCCGCTTTCGTACA
AGGCAAACTGCACGGCACCAATGCCCGCACGGCAGAGATGTGCAAACTGACGGAGAACTCCTCCCGCGACTCACAGATCG
CCTTTGCCAACGAACTCTCCATGATCTGCGACAAAGCCGGAATCAATGTATGGGAACTGATCGAACTGGCCAACAAGCAT
CCGCGTGTGAATATCCTTCAACCGGGCTGCGGAGTAGGCGGTCACTGCATTGCGGTAGACCCTTGGTTCATCGTATCCGA
CTATCCGGAACAGGCGCAGCTCATCAAGCGTGCCCGTGAAACGAACGATTACAAAGCGGACTGGTGTGCGAACAAAGTAT
TGGAAACCTGCCAGAAGTTTGTAGAACAGAACGAGCGCGAACCGGTCGTTGCCTGCATGGGACTGGCTTTCAAACCGAAT
ATCGATGACTTACGGGAATCTCCCGCCAAATATATCGCCTCCCGCATCATCTCCGAATCGAGAGCGGACGTGCTGATTAT
CGAACCTAATATAAGCACTCATAAGAGCTTCCATCTGACAGACTATCGGGAAGCATACGAGAAAGCGGATATTATCGTCT
GGCTGGTTCGCCACGATGTCTTCCTTGCATTGCCCAGGGAGGAAGGAAAGATAGAACTGGACTTCTGCGGTGTGAGACGA
TAA

Upstream 100 bases:

>100_bases
CAAAATGTTTGCACAAAAGTAGTGGTTTTCAGTGAAAATCCGTACAAAGTCACATATTATTTGTACTTTTGCCACACTTA
TTAATAAGGAGATATTGAAC

Downstream 100 bases:

>100_bases
AAATGTAGTTTAATTGCGAAGAGGTTATATAGACTCTATAAATAAAGCAGATGAAAAAGATATTGCTCGTTTTCGGTACC
CGTCCCGAAGCGATAAAGAT

Product: UDP-N-acetyl-D-mannosamine dehydrogenase

Products: UDP-N-acetyl-D-mannosaminuronic-acid; NADH; Proton [C]

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MKKVVFLGLGYIGLPTAAVAAAHGFEVIGVDVNPSVVETINQGKIHIVEPELGQVVRDVVQSGKLRAVCKPEAADAFFIV
VPTPFKQNHRADITYVEAATRSVIPYLQEGNLFVIESTSPVYTTERMAEVIYKERPELKGKIHIAYCPERVLPGNTLYEL
VHNDRVIGGIDPASTAKAIEFYSAFVQGKLHGTNARTAEMCKLTENSSRDSQIAFANELSMICDKAGINVWELIELANKH
PRVNILQPGCGVGGHCIAVDPWFIVSDYPEQAQLIKRARETNDYKADWCANKVLETCQKFVEQNEREPVVACMGLAFKPN
IDDLRESPAKYIASRIISESRADVLIIEPNISTHKSFHLTDYREAYEKADIIVWLVRHDVFLALPREEGKIELDFCGVRR

Sequences:

>Translated_400_residues
MKKVVFLGLGYIGLPTAAVAAAHGFEVIGVDVNPSVVETINQGKIHIVEPELGQVVRDVVQSGKLRAVCKPEAADAFFIV
VPTPFKQNHRADITYVEAATRSVIPYLQEGNLFVIESTSPVYTTERMAEVIYKERPELKGKIHIAYCPERVLPGNTLYEL
VHNDRVIGGIDPASTAKAIEFYSAFVQGKLHGTNARTAEMCKLTENSSRDSQIAFANELSMICDKAGINVWELIELANKH
PRVNILQPGCGVGGHCIAVDPWFIVSDYPEQAQLIKRARETNDYKADWCANKVLETCQKFVEQNEREPVVACMGLAFKPN
IDDLRESPAKYIASRIISESRADVLIIEPNISTHKSFHLTDYREAYEKADIIVWLVRHDVFLALPREEGKIELDFCGVRR
>Mature_400_residues
MKKVVFLGLGYIGLPTAAVAAAHGFEVIGVDVNPSVVETINQGKIHIVEPELGQVVRDVVQSGKLRAVCKPEAADAFFIV
VPTPFKQNHRADITYVEAATRSVIPYLQEGNLFVIESTSPVYTTERMAEVIYKERPELKGKIHIAYCPERVLPGNTLYEL
VHNDRVIGGIDPASTAKAIEFYSAFVQGKLHGTNARTAEMCKLTENSSRDSQIAFANELSMICDKAGINVWELIELANKH
PRVNILQPGCGVGGHCIAVDPWFIVSDYPEQAQLIKRARETNDYKADWCANKVLETCQKFVEQNEREPVVACMGLAFKPN
IDDLRESPAKYIASRIISESRADVLIIEPNISTHKSFHLTDYREAYEKADIIVWLVRHDVFLALPREEGKIELDFCGVRR

Specific function: Probably involved in the synthesis of sugar components of EPS I, by converting NDP-N-acetyl-D-galactosamine into NDP-N- acetyl-D-galactosaminuronic acid [H]

COG id: COG0677

COG function: function code M; UDP-N-acetyl-D-mannosaminuronate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4507813, Length=375, Percent_Identity=27.4666666666667, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI296040438, Length=275, Percent_Identity=25.4545454545455, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI296040443, Length=366, Percent_Identity=24.3169398907104, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI48994968, Length=394, Percent_Identity=54.5685279187817, Blast_Score=424, Evalue=1e-120,
Organism=Escherichia coli, GI1788340, Length=391, Percent_Identity=27.1099744245524, Blast_Score=99, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17560350, Length=398, Percent_Identity=26.1306532663317, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17136908, Length=367, Percent_Identity=28.6103542234332, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR017476
- InterPro:   IPR014027
- InterPro:   IPR014026
- InterPro:   IPR014028
- InterPro:   IPR001732 [H]

Pfam domain/function: PF00984 UDPG_MGDP_dh; PF03720 UDPG_MGDP_dh_C; PF03721 UDPG_MGDP_dh_N [H]

EC number: 1.1.1.- [C]

Molecular weight: Translated: 44650; Mature: 44650

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVVFLGLGYIGLPTAAVAAAHGFEVIGVDVNPSVVETINQGKIHIVEPELGQVVRDVV
CCEEEEEECCCCCCCHHHHHHHCCCEEEEECCCCHHHHHCCCCEEEEECCHHHHHHHHHH
QSGKLRAVCKPEAADAFFIVVPTPFKQNHRADITYVEAATRSVIPYLQEGNLFVIESTSP
HCCCEEEEECCCCCCEEEEEECCCCCCCCCCCEEEEEHHHHHHCCEEECCCEEEEECCCC
VYTTERMAEVIYKERPELKGKIHIAYCPERVLPGNTLYELVHNDRVIGGIDPASTAKAIE
CCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCEEEECCCCCHHHHHHH
FYSAFVQGKLHGTNARTAEMCKLTENSSRDSQIAFANELSMICDKAGINVWELIELANKH
HHHHHHHCEEECCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHCCC
PRVNILQPGCGVGGHCIAVDPWFIVSDYPEQAQLIKRARETNDYKADWCANKVLETCQKF
CCEEEECCCCCCCCEEEEECCEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VEQNEREPVVACMGLAFKPNIDDLRESPAKYIASRIISESRADVLIIEPNISTHKSFHLT
HHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEECC
DYREAYEKADIIVWLVRHDVFLALPREEGKIELDFCGVRR
HHHHHHCCCCEEEEEEECCEEEEECCCCCEEEEEEECCCC
>Mature Secondary Structure
MKKVVFLGLGYIGLPTAAVAAAHGFEVIGVDVNPSVVETINQGKIHIVEPELGQVVRDVV
CCEEEEEECCCCCCCHHHHHHHCCCEEEEECCCCHHHHHCCCCEEEEECCHHHHHHHHHH
QSGKLRAVCKPEAADAFFIVVPTPFKQNHRADITYVEAATRSVIPYLQEGNLFVIESTSP
HCCCEEEEECCCCCCEEEEEECCCCCCCCCCCEEEEEHHHHHHCCEEECCCEEEEECCCC
VYTTERMAEVIYKERPELKGKIHIAYCPERVLPGNTLYELVHNDRVIGGIDPASTAKAIE
CCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCEEEECCCCCHHHHHHH
FYSAFVQGKLHGTNARTAEMCKLTENSSRDSQIAFANELSMICDKAGINVWELIELANKH
HHHHHHHCEEECCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHCCC
PRVNILQPGCGVGGHCIAVDPWFIVSDYPEQAQLIKRARETNDYKADWCANKVLETCQKF
CCEEEECCCCCCCCEEEEECCEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VEQNEREPVVACMGLAFKPNIDDLRESPAKYIASRIISESRADVLIIEPNISTHKSFHLT
HHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEECC
DYREAYEKADIIVWLVRHDVFLALPREEGKIELDFCGVRR
HHHHHHCCCCEEEEEEECCEEEEECCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosamine; NAD [C]

Specific reaction: UDP-N-acetyl-D-mannosamine + NAD = UDP-N-acetyl-D-mannosaminuronic-acid + NADH + Proton [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]