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The map label for this gene is pyk [H]

Identifier: 29348250

GI number: 29348250

Start: 3543132

End: 3544589

Strand: Reverse

Name: pyk [H]

Synonym: BT_2841

Alternate gene names: 29348250

Gene position: 3544589-3543132 (Counterclockwise)

Preceding gene: 29348251

Following gene: 29348249

Centisome position: 56.62

GC content: 47.05

Gene sequence:

>1458_bases
ATGTTACTGAAACAGACAAAAATCGTCGCATCCATTTCGGACAGACGTTGCGATGTGGACTTTATAAAACAGCTGTTTGA
AGCTGGGATGAATGTGGTACGTATGAATACGGCTCACGCTAGCCGTGAAGGTTTTGAAGCGTTGATTGCCAATGTACGTT
CCGTATCCAACCGGATTGCAATCCTCATGGATACCAAAGGACCGGAAGTACGTACAACTGCCAATGCGGAACCGATTCCA
TATCAGATAGGTGATAAAGTAAAGATTGTCGGCAATCCTGATCAGGAGACGACTCGTGAATGTATCGCTGTTTCGTATCC
TAACTTTGTGAACGATTTGAATATCGGAGGTCTGGTTCTGATTGACGATGGTGATCTTGAACTGGAAGTAATCGATAAGA
CAGCCGATTATCTGCTTTGCGAAGTGAAAAACGATGCAACGTTGGGAAGCCGCAAGAGCGTGAACGTCCCGGGTGTTCGT
ATCAATCTTCCTTCATTGACAGAGAAAGACCGTAACAATATACTTTACGCGATTGAAAAGGATATCGATTTCATCGCTCA
CTCTTTCGTGCGCAACCGTCAGGACGTACTTGATATCCGTGAAATTCTGGATGCTCACAATAGTGATATCCGGATTATTG
CCAAGATTGAGAATCAGGAAGGGGTGGACAACATCGACGAGATTCTTGAAGTGGCGGACGGCGTGATGGTAGCCCGTGGT
GACCTGGGTATCGAAGTGCCGCAGGAACGTATTCCGGGTATCCAGCGTGTGCTGATCCGCAAATGTATCCTGGCAAAGAA
ACCGGTGATCGTTGCGACTCAGATGCTTCATACCATGATCAACAATCCTCGTCCGACCCGCGCGGAAGTGACCGATATCG
CGAATGCAATCTACTATCGTACGGATGCTCTGATGTTGAGCGGTGAGACTGCTTATGGCAAATATCCGGTAGATGCAGTC
AAGACAATGACCAAGATCGCGGCCCAGGCTGAAAAAGATAAACTGGAAGAGAATGATATCCGTATCCCGTTGAACGAAAA
CAGTAACGATGTAACGGCCTTCCTTGCTAAACAGGCAGTGAAAGCTACATCGAAACTGAAGATACGTGCCATCATCACAG
ACAGCTATAGCGGACGGACCGCCCGTAATCTGGCAGCTTTCCGTGGTAAGTTCCCGGTATTGGCTATCTGCTATAAAGAA
AAGACCATGCGTCATCTGGCACTTTCTTATGGAGTGGAAGCAATCTATATGCCGGAGCTGGCCAACGGACAGGAATATTA
TTTTGCCGCTTTGCGCCGCCTGTTGAAGGAAGGACGTTTGCAGCCGACTGATATGGTAGGATATCTGAGTAGCGGTAAGG
CAGGTACACAAACTTCATTCCTCGAAATCAATGTCGTGGAAGATGCGTTGAAACATGCGGGAGAAACTGTATTGCCTAAC
AACAATCGTTATCTCTAA

Upstream 100 bases:

>100_bases
GGAGTGATCTGTGGCTTCGGGCTGAACTCGTATCGGTTGGCTCTGGAGGCTTTGTTAGGTAATAAATAAGGTATTAATAT
ATTAAATAGGTAAAAAGGCT

Downstream 100 bases:

>100_bases
CTGTTACATCTAAAATAAGGTTTTTATGCAGGAGACTGAATCTATTGATGAATATATTTTGCAGCATATCGATGCGGAAA
GTGACTATCTGAAAGCACTC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MLLKQTKIVASISDRRCDVDFIKQLFEAGMNVVRMNTAHASREGFEALIANVRSVSNRIAILMDTKGPEVRTTANAEPIP
YQIGDKVKIVGNPDQETTRECIAVSYPNFVNDLNIGGLVLIDDGDLELEVIDKTADYLLCEVKNDATLGSRKSVNVPGVR
INLPSLTEKDRNNILYAIEKDIDFIAHSFVRNRQDVLDIREILDAHNSDIRIIAKIENQEGVDNIDEILEVADGVMVARG
DLGIEVPQERIPGIQRVLIRKCILAKKPVIVATQMLHTMINNPRPTRAEVTDIANAIYYRTDALMLSGETAYGKYPVDAV
KTMTKIAAQAEKDKLEENDIRIPLNENSNDVTAFLAKQAVKATSKLKIRAIITDSYSGRTARNLAAFRGKFPVLAICYKE
KTMRHLALSYGVEAIYMPELANGQEYYFAALRRLLKEGRLQPTDMVGYLSSGKAGTQTSFLEINVVEDALKHAGETVLPN
NNRYL

Sequences:

>Translated_485_residues
MLLKQTKIVASISDRRCDVDFIKQLFEAGMNVVRMNTAHASREGFEALIANVRSVSNRIAILMDTKGPEVRTTANAEPIP
YQIGDKVKIVGNPDQETTRECIAVSYPNFVNDLNIGGLVLIDDGDLELEVIDKTADYLLCEVKNDATLGSRKSVNVPGVR
INLPSLTEKDRNNILYAIEKDIDFIAHSFVRNRQDVLDIREILDAHNSDIRIIAKIENQEGVDNIDEILEVADGVMVARG
DLGIEVPQERIPGIQRVLIRKCILAKKPVIVATQMLHTMINNPRPTRAEVTDIANAIYYRTDALMLSGETAYGKYPVDAV
KTMTKIAAQAEKDKLEENDIRIPLNENSNDVTAFLAKQAVKATSKLKIRAIITDSYSGRTARNLAAFRGKFPVLAICYKE
KTMRHLALSYGVEAIYMPELANGQEYYFAALRRLLKEGRLQPTDMVGYLSSGKAGTQTSFLEINVVEDALKHAGETVLPN
NNRYL
>Mature_485_residues
MLLKQTKIVASISDRRCDVDFIKQLFEAGMNVVRMNTAHASREGFEALIANVRSVSNRIAILMDTKGPEVRTTANAEPIP
YQIGDKVKIVGNPDQETTRECIAVSYPNFVNDLNIGGLVLIDDGDLELEVIDKTADYLLCEVKNDATLGSRKSVNVPGVR
INLPSLTEKDRNNILYAIEKDIDFIAHSFVRNRQDVLDIREILDAHNSDIRIIAKIENQEGVDNIDEILEVADGVMVARG
DLGIEVPQERIPGIQRVLIRKCILAKKPVIVATQMLHTMINNPRPTRAEVTDIANAIYYRTDALMLSGETAYGKYPVDAV
KTMTKIAAQAEKDKLEENDIRIPLNENSNDVTAFLAKQAVKATSKLKIRAIITDSYSGRTARNLAAFRGKFPVLAICYKE
KTMRHLALSYGVEAIYMPELANGQEYYFAALRRLLKEGRLQPTDMVGYLSSGKAGTQTSFLEINVVEDALKHAGETVLPN
NNRYL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=430, Percent_Identity=39.3023255813954, Blast_Score=303, Evalue=2e-82,
Organism=Homo sapiens, GI33286420, Length=430, Percent_Identity=39.3023255813954, Blast_Score=303, Evalue=2e-82,
Organism=Homo sapiens, GI10835121, Length=430, Percent_Identity=40.6976744186046, Blast_Score=303, Evalue=3e-82,
Organism=Homo sapiens, GI32967597, Length=430, Percent_Identity=40.6976744186046, Blast_Score=302, Evalue=4e-82,
Organism=Homo sapiens, GI33286418, Length=431, Percent_Identity=39.2111368909513, Blast_Score=299, Evalue=4e-81,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=38.8888888888889, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=38.8888888888889, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=35.1694915254237, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=35.1694915254237, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=36.2790697674419, Blast_Score=142, Evalue=5e-34,
Organism=Escherichia coli, GI1787965, Length=463, Percent_Identity=40.8207343412527, Blast_Score=335, Evalue=3e-93,
Organism=Escherichia coli, GI1788160, Length=454, Percent_Identity=34.8017621145374, Blast_Score=232, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI17544584, Length=427, Percent_Identity=38.4074941451991, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI71984413, Length=438, Percent_Identity=40.1826484018265, Blast_Score=283, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17506831, Length=438, Percent_Identity=40.1826484018265, Blast_Score=282, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI71984406, Length=438, Percent_Identity=40.1826484018265, Blast_Score=282, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17506829, Length=438, Percent_Identity=40.1826484018265, Blast_Score=282, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6319279, Length=422, Percent_Identity=39.0995260663507, Blast_Score=279, Evalue=8e-76,
Organism=Saccharomyces cerevisiae, GI6324923, Length=437, Percent_Identity=36.3844393592677, Blast_Score=256, Evalue=8e-69,
Organism=Drosophila melanogaster, GI24648964, Length=428, Percent_Identity=39.7196261682243, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI28571814, Length=433, Percent_Identity=39.9538106235566, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI24648966, Length=451, Percent_Identity=35.920177383592, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24581235, Length=419, Percent_Identity=33.1742243436754, Blast_Score=224, Evalue=7e-59,
Organism=Drosophila melanogaster, GI24646914, Length=308, Percent_Identity=33.7662337662338, Blast_Score=166, Evalue=3e-41,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 53979; Mature: 53979

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKQTKIVASISDRRCDVDFIKQLFEAGMNVVRMNTAHASREGFEALIANVRSVSNRIA
CCCCCHHHHEECCCCCCCHHHHHHHHHCCCCEEEEECHHCCHHHHHHHHHHHHHHCCCEE
ILMDTKGPEVRTTANAEPIPYQIGDKVKIVGNPDQETTRECIAVSYPNFVNDLNIGGLVL
EEEECCCCCEEECCCCCCCCEECCCEEEEECCCCHHHHHHEEEEECCCHHCCCCCCCEEE
IDDGDLELEVIDKTADYLLCEVKNDATLGSRKSVNVPGVRINLPSLTEKDRNNILYAIEK
EECCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEC
DIDFIAHSFVRNRQDVLDIREILDAHNSDIRIIAKIENQEGVDNIDEILEVADGVMVARG
HHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHCCEEEEEC
DLGIEVPQERIPGIQRVLIRKCILAKKPVIVATQMLHTMINNPRPTRAEVTDIANAIYYR
CCCCCCCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEE
TDALMLSGETAYGKYPVDAVKTMTKIAAQAEKDKLEENDIRIPLNENSNDVTAFLAKQAV
ECEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHH
KATSKLKIRAIITDSYSGRTARNLAAFRGKFPVLAICYKEKTMRHLALSYGVEAIYMPEL
HHHCCEEEEEEEECCCCCCHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHCCCEEECCCC
ANGQEYYFAALRRLLKEGRLQPTDMVGYLSSGKAGTQTSFLEINVVEDALKHAGETVLPN
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHCCCEECCC
NNRYL
CCCCC
>Mature Secondary Structure
MLLKQTKIVASISDRRCDVDFIKQLFEAGMNVVRMNTAHASREGFEALIANVRSVSNRIA
CCCCCHHHHEECCCCCCCHHHHHHHHHCCCCEEEEECHHCCHHHHHHHHHHHHHHCCCEE
ILMDTKGPEVRTTANAEPIPYQIGDKVKIVGNPDQETTRECIAVSYPNFVNDLNIGGLVL
EEEECCCCCEEECCCCCCCCEECCCEEEEECCCCHHHHHHEEEEECCCHHCCCCCCCEEE
IDDGDLELEVIDKTADYLLCEVKNDATLGSRKSVNVPGVRINLPSLTEKDRNNILYAIEK
EECCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEC
DIDFIAHSFVRNRQDVLDIREILDAHNSDIRIIAKIENQEGVDNIDEILEVADGVMVARG
HHHHHHHHHHHCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHCCEEEEEC
DLGIEVPQERIPGIQRVLIRKCILAKKPVIVATQMLHTMINNPRPTRAEVTDIANAIYYR
CCCCCCCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEE
TDALMLSGETAYGKYPVDAVKTMTKIAAQAEKDKLEENDIRIPLNENSNDVTAFLAKQAV
ECEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHH
KATSKLKIRAIITDSYSGRTARNLAAFRGKFPVLAICYKEKTMRHLALSYGVEAIYMPEL
HHHCCEEEEEEEECCCCCCHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHCCCEEECCCC
ANGQEYYFAALRRLLKEGRLQPTDMVGYLSSGKAGTQTSFLEINVVEDALKHAGETVLPN
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCEEEEEEEHHHHHHHHCCCEECCC
NNRYL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]