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The map label for this gene is tag [H]

Identifier: 29347810

GI number: 29347810

Start: 2995904

End: 2996476

Strand: Reverse

Name: tag [H]

Synonym: BT_2400

Alternate gene names: 29347810

Gene position: 2996476-2995904 (Counterclockwise)

Preceding gene: 29347811

Following gene: 29347801

Centisome position: 47.86

GC content: 43.63

Gene sequence:

>573_bases
ATGCAAGATATAGTAAACGGACGCTGTGGTTGGTGTGGAACCGACGAACTGTATGTGAAATATCATGATCAGGAGTGGGG
AAATTTGGTCACTGACGACAAGACGCTGTTTGAGTTTCTTGTGCTGGAAAGTGCCCAGGCGGGGTTGAGCTGGATAACCA
TCCTCAGAAAACGGGAGGGTTATCGCAAAGCCTTTTGCGATTTTGATGCCGAACGGGTGGCACAGATGACTGATGAAGAT
GTAGAACGGTTAATGCACTTTGAAGGCATTGTGAAAAACCGTCTGAAAATTAAATCGACCATTACCAACGCTAGGCAATT
TCTTGCTATACAAAAGGAGTTCGGTAGTTTTTACGACTACACTCTCTCATTCTTTCCCGACAGAAAGCCTATTGTCAATA
CCTTTTGTTCATTGAGCGAGATTCCGGCATCTTCTCCCGAATCCGATGCAATGAGTAAGGACATGAAAAAGCGCGGATTC
AAATTCTTTGGAACGACGATTTGTTATGCTCACTTACAGGCTTCGGGATTTATCAACGATCACTTGAAGGATTGTATTTG
CCGGAAAAAATAA

Upstream 100 bases:

>100_bases
GAGAAAAAGAGCTTGCAAATGACGAAAGAATTTGCAGACTTCAAGAAGTATTTGCTTTCTGTTTGATTGGGTTTTAAAGC
AAGGTGTAAAAGAAATAATT

Downstream 100 bases:

>100_bases
ATGACGTGCGGAAATAATTCAAATCTGTTTAGGAATGAACTAAACAGTAGTACACAATAGTAACTCCATTCTCCTCCTTC
GGGAAGCTACCCTTTATACA

Product: DNA-3-methyladenine glycosylase I

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosylase I, constitutive; TAG I; DNA-3-methyladenine glycosidase I; DNA-3-methyladenine glycosylase I [H]

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MQDIVNGRCGWCGTDELYVKYHDQEWGNLVTDDKTLFEFLVLESAQAGLSWITILRKREGYRKAFCDFDAERVAQMTDED
VERLMHFEGIVKNRLKIKSTITNARQFLAIQKEFGSFYDYTLSFFPDRKPIVNTFCSLSEIPASSPESDAMSKDMKKRGF
KFFGTTICYAHLQASGFINDHLKDCICRKK

Sequences:

>Translated_190_residues
MQDIVNGRCGWCGTDELYVKYHDQEWGNLVTDDKTLFEFLVLESAQAGLSWITILRKREGYRKAFCDFDAERVAQMTDED
VERLMHFEGIVKNRLKIKSTITNARQFLAIQKEFGSFYDYTLSFFPDRKPIVNTFCSLSEIPASSPESDAMSKDMKKRGF
KFFGTTICYAHLQASGFINDHLKDCICRKK
>Mature_190_residues
MQDIVNGRCGWCGTDELYVKYHDQEWGNLVTDDKTLFEFLVLESAQAGLSWITILRKREGYRKAFCDFDAERVAQMTDED
VERLMHFEGIVKNRLKIKSTITNARQFLAIQKEFGSFYDYTLSFFPDRKPIVNTFCSLSEIPASSPESDAMSKDMKKRGF
KFFGTTICYAHLQASGFINDHLKDCICRKK

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=180, Percent_Identity=52.7777777777778, Blast_Score=201, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 22006; Mature: 22006

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDIVNGRCGWCGTDELYVKYHDQEWGNLVTDDKTLFEFLVLESAQAGLSWITILRKREG
CCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YRKAFCDFDAERVAQMTDEDVERLMHFEGIVKNRLKIKSTITNARQFLAIQKEFGSFYDY
HHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
TLSFFPDRKPIVNTFCSLSEIPASSPESDAMSKDMKKRGFKFFGTTICYAHLQASGFIND
HHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHH
HLKDCICRKK
HHHHHHHCCC
>Mature Secondary Structure
MQDIVNGRCGWCGTDELYVKYHDQEWGNLVTDDKTLFEFLVLESAQAGLSWITILRKREG
CCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YRKAFCDFDAERVAQMTDEDVERLMHFEGIVKNRLKIKSTITNARQFLAIQKEFGSFYDY
HHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
TLSFFPDRKPIVNTFCSLSEIPASSPESDAMSKDMKKRGFKFFGTTICYAHLQASGFIND
HHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHH
HLKDCICRKK
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536912; 3520491; 8041620; 9278503 [H]