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The map label for this gene is mscS [H]

Identifier: 29347799

GI number: 29347799

Start: 2976688

End: 2977599

Strand: Reverse

Name: mscS [H]

Synonym: BT_2389

Alternate gene names: 29347799

Gene position: 2977599-2976688 (Counterclockwise)

Preceding gene: 29347801

Following gene: 29347796

Centisome position: 47.56

GC content: 45.94

Gene sequence:

>912_bases
ATGCTACTATTATTTCAAGCGACAGCAACACAGCTTGCGGATTCTACACAAATCGTCGCAGATAAATTAATGGAAGAAGC
GATAGCCAATGCGGACGGATTGGATAAGCTTTCTCTGATCACCCAGCAATTGGTAGATTTCGGTATCCGTGCCGGAGAAC
GCATCCTGATTGCAACCCTTGTCTTCATTGTCGGACGCTTCCTCATCTCGATGCTCAACCGATTTGTCGGACGTCTGATG
GACAGGAGAAAGGTGGATATAAGCATCAAGACTTTTGTGAAGAGTCTGGTAAATATTCTGCTGACCATTCTGTTGATTAT
CTCCGTAGTCGGTGCTCTGGGAGTCGAAACAACTTCTTTTGCAGCCCTGTTGGCTTCTGCCGGTGTGGCTGTCGGTATGG
CGTTGTCCGGTAACTTGCAGAACTTTGCCGGCGGACTGATCGTATTGCTTTTCAAGCCTTATAAGGTAGGAGATTGGATT
GAAAGTCAGGGAGTTTCCGGAACGGTCAAGGAAATACAGATATTTCACACCATTCTGACTACCGGAGATAATAAAGTGAT
TTATATACCGAACGGTGCAATGAGCAGCGGAGTGGTGACAAACTATAATACACAGACAACGCGCCGTGTAGAATGGATTG
TTGGCGTGGATTATGGAGAAGATTATAATAAGGTACAGCAGATTGTCCGTGATATACTGACGGCCGACAATCGTATTCTG
ACTGATCCCGCCCCGTTTATTGCCCTTCATGCGCTGGATGCCAGCAGTGTGAATGTGGTAGCCCGTGTGTGGGTGAATAC
GGCAGATTACTGGGGAGTTTATTTTGATATAAACAAGACTATTTATGAAACCTTTAATGAAAAAGGCATCAACTTCCCGT
TCCCGCAACTTACCGTGCATCAGGGAAACTGA

Upstream 100 bases:

>100_bases
GCAAAAAAAACTTTTAGATTTGCTAAAAACAATATAACTTTGTAGTTTGTTGAAATATGACAAGTCAGCTATTTATTCAT
TTTAATATAATTATCAAATT

Downstream 100 bases:

>100_bases
TCACTATAAGATATATACTAAAACGAGAGAGTTTCACTTCGGTGAGACTCTCTCGTTTTATATGAACAAATTGTCTGGTG
TGATTGGAAAATTTCTTTTT

Product: putative transport protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MLLLFQATATQLADSTQIVADKLMEEAIANADGLDKLSLITQQLVDFGIRAGERILIATLVFIVGRFLISMLNRFVGRLM
DRRKVDISIKTFVKSLVNILLTILLIISVVGALGVETTSFAALLASAGVAVGMALSGNLQNFAGGLIVLLFKPYKVGDWI
ESQGVSGTVKEIQIFHTILTTGDNKVIYIPNGAMSSGVVTNYNTQTTRRVEWIVGVDYGEDYNKVQQIVRDILTADNRIL
TDPAPFIALHALDASSVNVVARVWVNTADYWGVYFDINKTIYETFNEKGINFPFPQLTVHQGN

Sequences:

>Translated_303_residues
MLLLFQATATQLADSTQIVADKLMEEAIANADGLDKLSLITQQLVDFGIRAGERILIATLVFIVGRFLISMLNRFVGRLM
DRRKVDISIKTFVKSLVNILLTILLIISVVGALGVETTSFAALLASAGVAVGMALSGNLQNFAGGLIVLLFKPYKVGDWI
ESQGVSGTVKEIQIFHTILTTGDNKVIYIPNGAMSSGVVTNYNTQTTRRVEWIVGVDYGEDYNKVQQIVRDILTADNRIL
TDPAPFIALHALDASSVNVVARVWVNTADYWGVYFDINKTIYETFNEKGINFPFPQLTVHQGN
>Mature_303_residues
MLLLFQATATQLADSTQIVADKLMEEAIANADGLDKLSLITQQLVDFGIRAGERILIATLVFIVGRFLISMLNRFVGRLM
DRRKVDISIKTFVKSLVNILLTILLIISVVGALGVETTSFAALLASAGVAVGMALSGNLQNFAGGLIVLLFKPYKVGDWI
ESQGVSGTVKEIQIFHTILTTGDNKVIYIPNGAMSSGVVTNYNTQTTRRVEWIVGVDYGEDYNKVQQIVRDILTADNRIL
TDPAPFIALHALDASSVNVVARVWVNTADYWGVYFDINKTIYETFNEKGINFPFPQLTVHQGN

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=246, Percent_Identity=39.8373983739837, Blast_Score=205, Evalue=3e-54,
Organism=Escherichia coli, GI1786670, Length=213, Percent_Identity=31.924882629108, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787591, Length=276, Percent_Identity=27.536231884058, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI2367355, Length=250, Percent_Identity=27.6, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 33232; Mature: 33232

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLLFQATATQLADSTQIVADKLMEEAIANADGLDKLSLITQQLVDFGIRAGERILIATL
CEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
VFIVGRFLISMLNRFVGRLMDRRKVDISIKTFVKSLVNILLTILLIISVVGALGVETTSF
HHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
AALLASAGVAVGMALSGNLQNFAGGLIVLLFKPYKVGDWIESQGVSGTVKEIQIFHTILT
HHHHHHHHHHEEEEECCCHHHHCCCEEEEEECCCCCCCHHHHCCCCCHHHHHHHHHHHHC
TGDNKVIYIPNGAMSSGVVTNYNTQTTRRVEWIVGVDYGEDYNKVQQIVRDILTADNRIL
CCCCEEEEECCCCCCCCEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEE
TDPAPFIALHALDASSVNVVARVWVNTADYWGVYFDINKTIYETFNEKGINFPFPQLTVH
CCCCCEEEEEECCCCCHHEEEEEEECCCCCEEEEEECCHHHHHHHHCCCCCCCCCCEEEE
QGN
CCC
>Mature Secondary Structure
MLLLFQATATQLADSTQIVADKLMEEAIANADGLDKLSLITQQLVDFGIRAGERILIATL
CEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
VFIVGRFLISMLNRFVGRLMDRRKVDISIKTFVKSLVNILLTILLIISVVGALGVETTSF
HHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
AALLASAGVAVGMALSGNLQNFAGGLIVLLFKPYKVGDWIESQGVSGTVKEIQIFHTILT
HHHHHHHHHHEEEEECCCHHHHCCCEEEEEECCCCCCCHHHHCCCCCHHHHHHHHHHHHC
TGDNKVIYIPNGAMSSGVVTNYNTQTTRRVEWIVGVDYGEDYNKVQQIVRDILTADNRIL
CCCCEEEEECCCCCCCCEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEE
TDPAPFIALHALDASSVNVVARVWVNTADYWGVYFDINKTIYETFNEKGINFPFPQLTVH
CCCCCEEEEEECCCCCHHEEEEEEECCCCCEEEEEECCHHHHHHHHCCCCCCCCCCEEEE
QGN
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]