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The map label for this gene is acnA [H]

Identifier: 29347482

GI number: 29347482

Start: 2594292

End: 2596535

Strand: Reverse

Name: acnA [H]

Synonym: BT_2072

Alternate gene names: 29347482

Gene position: 2596535-2594292 (Counterclockwise)

Preceding gene: 29347484

Following gene: 29347481

Centisome position: 41.48

GC content: 52.45

Gene sequence:

>2244_bases
ATGGTGTATGATTTGACTATGTTAGAGGCTTTTTATTCTGCTTATAAAGGAAAAGTGGAACACGTGCGGGCTGTTTTGAA
ACGCCCATTGACGTTAGCCGAGAAGATTCTGTATGCCCATCTGTTCAATGAGGGCGATCTGAAAAACTACAAGCGGGGTG
AAGACTACGTAAATTTCCGTCCCGACCGCGTCGCTATGCAGGACGCGACTGCCCAGATGGCATTGCTTCAATTCATGAAT
GCAGGAAAAGAAAAAGTGGCTGTTCCCTCCACCGTGCACTGTGACCACTTGATACAAGCGTACAAAGGAGCAAAGGAGGA
CATTGCTACGGCAACCAAAACAAACGAAGAAGTTTACGACTTTTTGCGCGATGTATCCTCTCGTTACGGCATCGGCTTCT
GGAAGCCGGGCGCCGGAATCATTCACCAGGTAGTGCTCGAAAATTACGCTTTCCCCGGCGGGATGATGGTAGGAACCGAC
TCTCACACTCCGAATGCCGGAGGACTGGGCATGGTGGCCATCGGCGTCGGCGGTGCGGATGCCGTAGACGTGATGACGGG
TATGGAATGGGAACTGAAAATGCCAAGGATTATCGGTGTCCGCCTGACCGGAAAACTGAGCGGCTGGACTTCCCCGAAAG
ATGTGATCCTGAAACTTGCTGGGATTCTGACCGTAAAAGGAGGAACGAACTCCATCATCGAATATTTCGGACCGGGAACA
GCTTCCCTGTCGGCTACCGGCAAAGCGACCATTTGCAATATGGGTGCGGAAGTAGGAGCTACCACTTCGCTTTTCCCGTT
CGACGGGCGCATGGCTACTTATCTGAGAGCCACCGGCAGGGATCGCATCGTGGAACTGGCGGAAGCCGTGGACTGTGAGC
TCCGTGCGGACCAACAGGTGACGGACGAACCGGAAAAGTACTACGACCGTGTCATCGACATTGACTTGTCCACGCTCGAA
CCTTATATCAACGGCCCTTTCACACCGGATGCCGCTACCCCTATCTCTGAATTTGCAGAAAAAGTATTACTGAACGGCTA
TCCGCGTAAGATGGAAGTCGGACTGATCGGTTCGTGTACCAACTCTTCTTACCAAGATCTCAGCCGTGCCGCATCCCTTG
CAAGGCAGGTGAAGGAAAAGAATCTGAGTGTGGCTTCCCCGCTGATTATCAATCCGGGTTCCGAGCAAATCCGCGCTACT
GCCGAAAGAGACGGAATGATGGATGACTTCATGCAGATCGGCGCCGTGATCATGGCAAATGCCTGCGGTCCTTGCATCGG
TCAGTGGAAACGTCATACGGACGACCCGACACGGAAGAACTCGATCGTCACATCCTTCAACCGTAACTTTGCCAAACGTG
CGGACGGCAATCCGAACACATACGCCTTTGTTGCTTCTCCCGAACTGACGATGGCACTGACCATTGCCGGTGATCTTTGC
TTCAATCCGCTGAAAGACAGACTGATGAACCATGACGGCGAGAAAGTGAAACTGGCCGAACCCGTAGGAGATGAACTCCC
CTTGAGAGGTTTCACCTCCGGCAATGAAGGCTATATCACCCCCGGCGGGACGAAAACAGCGATCAACGTCAACCCGGCTT
CTCAACGCCTGCAACTGCTGACTCCTTTCCCCGCTTGGGACGGACAAGATATACTCAATATGCCCCTGCTGATTAAGGCG
CAGGGCAAATGTACTACCGACCATATTTCGATGGCGGGACCGTGGCTGCGTTTCCGCGGACATTTGGAGAATATTTCGGA
TAATATGCTAATGGGAGCCGTGAATGCGTTCAATGGCGAAACGAACAACGTGTGGAACCGTTCGACAAACACCTACGGAA
CCGTTTCGGGCACAGCCAAAATGTATAAATCCGAAGGAATCCCCTCTATTGTCGTGGCCGAAGAAAACTATGGAGAAGGC
TCCAGTCGCGAGCACGCGGCAATGGAACCCCGTTTCCTGAATGTGCGTGTGATTCTCGCCAAGAGCTTCGCCCGTATCCA
CGAAACGAATCTGAAAAAGCAGGGCATGCTTGCCCTTACCTTTGCAGACAAAGCGGATTATGACAAGATACAGGAGCACG
ACCTCCTTTCAGTCATCGGCTTGCCGGACTTTGCTCCGGGACGGAATCTGACCGTCGTTCTCCATCACGAAGACGGAACG
AAAGAGAGTTTTGAAGCGCAACATACATACAACGAACAGCAGATTGCCTGGTTCCGTGCCGGCTCTGCCTTGAATGCCAG
ATAA

Upstream 100 bases:

>100_bases
TAAATCTTCTTCCTGAGACGAAAATATATTTCATTTTGTTAAACATTCCTCGAACGCCGTTCGTTATTTTTATAGATAGA
CTAAAAAAAATAAAAGCAAT

Downstream 100 bases:

>100_bases
TTAAAATGATAGAAATGAACAAAATAACCATGCAAAAAGATGGTACGCTATCGGTGCCCGATGTGCCTGTCGTACCTTAT
ATTACCGGAGACGGAGTAGG

Product: aconitate hydratase

Products: NA

Alternate protein names: Aconitase; Citrate hydro-lyase [H]

Number of amino acids: Translated: 747; Mature: 747

Protein sequence:

>747_residues
MVYDLTMLEAFYSAYKGKVEHVRAVLKRPLTLAEKILYAHLFNEGDLKNYKRGEDYVNFRPDRVAMQDATAQMALLQFMN
AGKEKVAVPSTVHCDHLIQAYKGAKEDIATATKTNEEVYDFLRDVSSRYGIGFWKPGAGIIHQVVLENYAFPGGMMVGTD
SHTPNAGGLGMVAIGVGGADAVDVMTGMEWELKMPRIIGVRLTGKLSGWTSPKDVILKLAGILTVKGGTNSIIEYFGPGT
ASLSATGKATICNMGAEVGATTSLFPFDGRMATYLRATGRDRIVELAEAVDCELRADQQVTDEPEKYYDRVIDIDLSTLE
PYINGPFTPDAATPISEFAEKVLLNGYPRKMEVGLIGSCTNSSYQDLSRAASLARQVKEKNLSVASPLIINPGSEQIRAT
AERDGMMDDFMQIGAVIMANACGPCIGQWKRHTDDPTRKNSIVTSFNRNFAKRADGNPNTYAFVASPELTMALTIAGDLC
FNPLKDRLMNHDGEKVKLAEPVGDELPLRGFTSGNEGYITPGGTKTAINVNPASQRLQLLTPFPAWDGQDILNMPLLIKA
QGKCTTDHISMAGPWLRFRGHLENISDNMLMGAVNAFNGETNNVWNRSTNTYGTVSGTAKMYKSEGIPSIVVAEENYGEG
SSREHAAMEPRFLNVRVILAKSFARIHETNLKKQGMLALTFADKADYDKIQEHDLLSVIGLPDFAPGRNLTVVLHHEDGT
KESFEAQHTYNEQQIAWFRAGSALNAR

Sequences:

>Translated_747_residues
MVYDLTMLEAFYSAYKGKVEHVRAVLKRPLTLAEKILYAHLFNEGDLKNYKRGEDYVNFRPDRVAMQDATAQMALLQFMN
AGKEKVAVPSTVHCDHLIQAYKGAKEDIATATKTNEEVYDFLRDVSSRYGIGFWKPGAGIIHQVVLENYAFPGGMMVGTD
SHTPNAGGLGMVAIGVGGADAVDVMTGMEWELKMPRIIGVRLTGKLSGWTSPKDVILKLAGILTVKGGTNSIIEYFGPGT
ASLSATGKATICNMGAEVGATTSLFPFDGRMATYLRATGRDRIVELAEAVDCELRADQQVTDEPEKYYDRVIDIDLSTLE
PYINGPFTPDAATPISEFAEKVLLNGYPRKMEVGLIGSCTNSSYQDLSRAASLARQVKEKNLSVASPLIINPGSEQIRAT
AERDGMMDDFMQIGAVIMANACGPCIGQWKRHTDDPTRKNSIVTSFNRNFAKRADGNPNTYAFVASPELTMALTIAGDLC
FNPLKDRLMNHDGEKVKLAEPVGDELPLRGFTSGNEGYITPGGTKTAINVNPASQRLQLLTPFPAWDGQDILNMPLLIKA
QGKCTTDHISMAGPWLRFRGHLENISDNMLMGAVNAFNGETNNVWNRSTNTYGTVSGTAKMYKSEGIPSIVVAEENYGEG
SSREHAAMEPRFLNVRVILAKSFARIHETNLKKQGMLALTFADKADYDKIQEHDLLSVIGLPDFAPGRNLTVVLHHEDGT
KESFEAQHTYNEQQIAWFRAGSALNAR
>Mature_747_residues
MVYDLTMLEAFYSAYKGKVEHVRAVLKRPLTLAEKILYAHLFNEGDLKNYKRGEDYVNFRPDRVAMQDATAQMALLQFMN
AGKEKVAVPSTVHCDHLIQAYKGAKEDIATATKTNEEVYDFLRDVSSRYGIGFWKPGAGIIHQVVLENYAFPGGMMVGTD
SHTPNAGGLGMVAIGVGGADAVDVMTGMEWELKMPRIIGVRLTGKLSGWTSPKDVILKLAGILTVKGGTNSIIEYFGPGT
ASLSATGKATICNMGAEVGATTSLFPFDGRMATYLRATGRDRIVELAEAVDCELRADQQVTDEPEKYYDRVIDIDLSTLE
PYINGPFTPDAATPISEFAEKVLLNGYPRKMEVGLIGSCTNSSYQDLSRAASLARQVKEKNLSVASPLIINPGSEQIRAT
AERDGMMDDFMQIGAVIMANACGPCIGQWKRHTDDPTRKNSIVTSFNRNFAKRADGNPNTYAFVASPELTMALTIAGDLC
FNPLKDRLMNHDGEKVKLAEPVGDELPLRGFTSGNEGYITPGGTKTAINVNPASQRLQLLTPFPAWDGQDILNMPLLIKA
QGKCTTDHISMAGPWLRFRGHLENISDNMLMGAVNAFNGETNNVWNRSTNTYGTVSGTAKMYKSEGIPSIVVAEENYGEG
SSREHAAMEPRFLNVRVILAKSFARIHETNLKKQGMLALTFADKADYDKIQEHDLLSVIGLPDFAPGRNLTVVLHHEDGT
KESFEAQHTYNEQQIAWFRAGSALNAR

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate [H]

COG id: COG1048

COG function: function code C; Aconitase A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=736, Percent_Identity=57.3369565217391, Blast_Score=860, Evalue=0.0,
Organism=Homo sapiens, GI8659555, Length=832, Percent_Identity=27.7644230769231, Blast_Score=219, Evalue=6e-57,
Organism=Homo sapiens, GI41352693, Length=423, Percent_Identity=28.8416075650118, Blast_Score=139, Evalue=1e-32,
Organism=Escherichia coli, GI1787531, Length=808, Percent_Identity=26.980198019802, Blast_Score=173, Evalue=4e-44,
Organism=Escherichia coli, GI87081781, Length=455, Percent_Identity=25.9340659340659, Blast_Score=142, Evalue=1e-34,
Organism=Escherichia coli, GI1786259, Length=372, Percent_Identity=25.8064516129032, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25149337, Length=734, Percent_Identity=57.3569482288828, Blast_Score=867, Evalue=0.0,
Organism=Caenorhabditis elegans, GI32564738, Length=680, Percent_Identity=58.0882352941177, Blast_Score=820, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25149342, Length=570, Percent_Identity=56.140350877193, Blast_Score=672, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17568399, Length=842, Percent_Identity=28.1472684085511, Blast_Score=217, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6323335, Length=736, Percent_Identity=57.3369565217391, Blast_Score=876, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322261, Length=737, Percent_Identity=53.7313432835821, Blast_Score=785, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320440, Length=440, Percent_Identity=27.7272727272727, Blast_Score=152, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6321429, Length=469, Percent_Identity=26.0127931769723, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI281365315, Length=737, Percent_Identity=57.9375848032564, Blast_Score=853, Evalue=0.0,
Organism=Drosophila melanogaster, GI17864292, Length=737, Percent_Identity=57.9375848032564, Blast_Score=853, Evalue=0.0,
Organism=Drosophila melanogaster, GI28571643, Length=736, Percent_Identity=56.5217391304348, Blast_Score=833, Evalue=0.0,
Organism=Drosophila melanogaster, GI161076999, Length=683, Percent_Identity=58.8579795021962, Blast_Score=805, Evalue=0.0,
Organism=Drosophila melanogaster, GI17137564, Length=832, Percent_Identity=27.2836538461538, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24645686, Length=827, Percent_Identity=27.4486094316808, Blast_Score=191, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR006249
- InterPro:   IPR015934
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00330 Aconitase; PF00694 Aconitase_C [H]

EC number: =4.2.1.3 [H]

Molecular weight: Translated: 81813; Mature: 81813

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYDLTMLEAFYSAYKGKVEHVRAVLKRPLTLAEKILYAHLFNEGDLKNYKRGEDYVNFR
CEEEHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PDRVAMQDATAQMALLQFMNAGKEKVAVPSTVHCDHLIQAYKGAKEDIATATKTNEEVYD
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCHHHHH
FLRDVSSRYGIGFWKPGAGIIHQVVLENYAFPGGMMVGTDSHTPNAGGLGMVAIGVGGAD
HHHHHHHHCCCCEECCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEECCCCC
AVDVMTGMEWELKMPRIIGVRLTGKLSGWTSPKDVILKLAGILTVKGGTNSIIEYFGPGT
HHHHHCCCCEEEECCEEEEEEEEEECCCCCCHHHHHHHHHHEEEECCCCCHHHHHCCCCC
ASLSATGKATICNMGAEVGATTSLFPFDGRMATYLRATGRDRIVELAEAVDCELRADQQV
CEECCCCCEEEEECCCCCCCCCEEECCCCCCEEHHHHCCHHHHHHHHHHHCCHHCCCCCC
TDEPEKYYDRVIDIDLSTLEPYINGPFTPDAATPISEFAEKVLLNGYPRKMEVGLIGSCT
CCCHHHHHHHEEEEEHHHCCHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEECCC
NSSYQDLSRAASLARQVKEKNLSVASPLIINPGSEQIRATAERDGMMDDFMQIGAVIMAN
CCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHC
ACGPCIGQWKRHTDDPTRKNSIVTSFNRNFAKRADGNPNTYAFVASPELTMALTIAGDLC
CCCHHHHHHHHCCCCCCCCCCEEEHHCCHHHHHCCCCCCEEEEEECCCEEEEEEEECHHH
FNPLKDRLMNHDGEKVKLAEPVGDELPLRGFTSGNEGYITPGGTKTAINVNPASQRLQLL
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEECCCCCEEEEECCCHHHCEEEE
TPFPAWDGQDILNMPLLIKAQGKCTTDHISMAGPWLRFRGHLENISDNMLMGAVNAFNGE
ECCCCCCCCHHHCCCEEEEECCCCCCCCHHCCCCHHHHHHHHHCCCCCCEEEEHHHCCCC
TNNVWNRSTNTYGTVSGTAKMYKSEGIPSIVVAEENYGEGSSREHAAMEPRFLNVRVILA
CCCCCCCCCCCEEEECCHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEEEH
KSFARIHETNLKKQGMLALTFADKADYDKIQEHDLLSVIGLPDFAPGRNLTVVLHHEDGT
HHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHCCCCCEECCCCCCCCCCEEEEEECCCCC
KESFEAQHTYNEQQIAWFRAGSALNAR
CHHHHHHHCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MVYDLTMLEAFYSAYKGKVEHVRAVLKRPLTLAEKILYAHLFNEGDLKNYKRGEDYVNFR
CEEEHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PDRVAMQDATAQMALLQFMNAGKEKVAVPSTVHCDHLIQAYKGAKEDIATATKTNEEVYD
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCHHHHH
FLRDVSSRYGIGFWKPGAGIIHQVVLENYAFPGGMMVGTDSHTPNAGGLGMVAIGVGGAD
HHHHHHHHCCCCEECCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEECCCCC
AVDVMTGMEWELKMPRIIGVRLTGKLSGWTSPKDVILKLAGILTVKGGTNSIIEYFGPGT
HHHHHCCCCEEEECCEEEEEEEEEECCCCCCHHHHHHHHHHEEEECCCCCHHHHHCCCCC
ASLSATGKATICNMGAEVGATTSLFPFDGRMATYLRATGRDRIVELAEAVDCELRADQQV
CEECCCCCEEEEECCCCCCCCCEEECCCCCCEEHHHHCCHHHHHHHHHHHCCHHCCCCCC
TDEPEKYYDRVIDIDLSTLEPYINGPFTPDAATPISEFAEKVLLNGYPRKMEVGLIGSCT
CCCHHHHHHHEEEEEHHHCCHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEECCC
NSSYQDLSRAASLARQVKEKNLSVASPLIINPGSEQIRATAERDGMMDDFMQIGAVIMAN
CCHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHC
ACGPCIGQWKRHTDDPTRKNSIVTSFNRNFAKRADGNPNTYAFVASPELTMALTIAGDLC
CCCHHHHHHHHCCCCCCCCCCEEEHHCCHHHHHCCCCCCEEEEEECCCEEEEEEEECHHH
FNPLKDRLMNHDGEKVKLAEPVGDELPLRGFTSGNEGYITPGGTKTAINVNPASQRLQLL
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEECCCCCEEEEECCCHHHCEEEE
TPFPAWDGQDILNMPLLIKAQGKCTTDHISMAGPWLRFRGHLENISDNMLMGAVNAFNGE
ECCCCCCCCHHHCCCEEEEECCCCCCCCHHCCCCHHHHHHHHHCCCCCCEEEEHHHCCCC
TNNVWNRSTNTYGTVSGTAKMYKSEGIPSIVVAEENYGEGSSREHAAMEPRFLNVRVILA
CCCCCCCCCCCEEEECCHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEEEH
KSFARIHETNLKKQGMLALTFADKADYDKIQEHDLLSVIGLPDFAPGRNLTVVLHHEDGT
HHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHCCCCCEECCCCCCCCCCEEEEEECCCCC
KESFEAQHTYNEQQIAWFRAGSALNAR
CHHHHHHHCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA