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The map label for this gene is purA [H]

Identifier: 29347253

GI number: 29347253

Start: 2314782

End: 2316053

Strand: Reverse

Name: purA [H]

Synonym: BT_1843

Alternate gene names: 29347253

Gene position: 2316053-2314782 (Counterclockwise)

Preceding gene: 29347254

Following gene: 29347252

Centisome position: 37.0

GC content: 45.83

Gene sequence:

>1272_bases
ATGAAAGTAGATGTTCTATTAGGATTACAATGGGGCGACGAAGGTAAAGGCAAAGTAGTCGACGTATTAACACCTAAGTA
CGATGTGGTCGCTCGTTTTCAGGGCGGTCCGAATGCCGGTCATACACTTGAGTTCGAAGGACAGAAGTATGTACTTCGTT
CTATTCCTTCCGGTATTTTTCAGGGAGATAAGGTAAATATTATCGGTAACGGTGTAGTGCTCGATCCGGCATTGTTCAAA
GCGGAAGCTGAGGCACTCGAAGCATCGGGACACCCATTGAAAGAGCGTTTGCACATTTCTAAGAAAGCTCATCTCATCCT
TCCGACACACCGCATACTGGACGCTGCTTACGAAGCAGCCAAGGGAGATGCGAAAGTAGGAACAACCGGTAAAGGCATTG
GCCCTACTTATACGGATAAAGTTAGCCGTAACGGTGTTCGTGTAGGTGATATCCTTCATAATTTCGAACAGAAATACGGT
GCGGCGAAAGCTCGTCACGAACAAATACTGAAGAGTCTGAACTACGAATATGACCTCACAGAACTGGAAAAAGCATGGAT
GGAAGGTATCGAATATCTGAAACAGTTCCATTTTGTAGACAGTGAGCATGAGGTTAACAATTATCTGAAAGACGGCAAGA
GCGTTCTTTGCGAAGGTGCTCAGGGAACGATGCTTGATATCGATTTCGGTTCGTATCCGTTTGTTACTTCTTCCAATACG
GTTTGTGCAGGTGCCTGCACCGGTCTGGGAGTAGCTCCGAACCGTATTGGCGAAGTGTTCGGTATCTTCAAGGCATACTG
TACTCGTGTAGGTGCCGGTCCGTTCCCGACAGAACTGTTCGACGAGACTGGTGACAAGATGTGTACATTGGGACATGAAT
TCGGCTCTGTAACAGGACGTAAACGCCGTTGCGGATGGATTGACCTGGTAGCTCTGAAATATTCCGTTATGATAAACGGT
GTTACCAAACTGATCATGATGAAGAGCGACGTGCTCGATACATTCGATACCATCAAGGCTTGTGTTGCTTATAAGGTAGA
TGGCGAGGAAATTGATTATTTCCCATATGATATCACTGAAGGTGTGGAGCCTGTATATGCAGAACTGCCGGGCTGGAAGA
CAGATATGACAAAGATGCAGAGCGAAGATGAATTCCCGGAAGAATTCAATGCTTATCTGACTTTCCTGGAAGAACAACTT
GGTGTGGAAATCAAGATTGTATCTGTCGGACCGGACAGAGCCCAGACAATCGAACGTTATACTGAAGAATAA

Upstream 100 bases:

>100_bases
TTCAACTTTCGCATTACTCCTTATATATATATGGATTGTGCAGCAAGTGCGACAGAGCTAATAAACGGAAAAAAGTCAGT
AACAACAATAAAAAAGAAAA

Downstream 100 bases:

>100_bases
GAAATGTTATTCTATTCCTAGAATAACTTTGTTCTATAACTTAATGAGAGGCGGAGAAATGGAAATGACTCCGCCTTTTT
GTTTTCTCTTTACCCCCAAT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MKVDVLLGLQWGDEGKGKVVDVLTPKYDVVARFQGGPNAGHTLEFEGQKYVLRSIPSGIFQGDKVNIIGNGVVLDPALFK
AEAEALEASGHPLKERLHISKKAHLILPTHRILDAAYEAAKGDAKVGTTGKGIGPTYTDKVSRNGVRVGDILHNFEQKYG
AAKARHEQILKSLNYEYDLTELEKAWMEGIEYLKQFHFVDSEHEVNNYLKDGKSVLCEGAQGTMLDIDFGSYPFVTSSNT
VCAGACTGLGVAPNRIGEVFGIFKAYCTRVGAGPFPTELFDETGDKMCTLGHEFGSVTGRKRRCGWIDLVALKYSVMING
VTKLIMMKSDVLDTFDTIKACVAYKVDGEEIDYFPYDITEGVEPVYAELPGWKTDMTKMQSEDEFPEEFNAYLTFLEEQL
GVEIKIVSVGPDRAQTIERYTEE

Sequences:

>Translated_423_residues
MKVDVLLGLQWGDEGKGKVVDVLTPKYDVVARFQGGPNAGHTLEFEGQKYVLRSIPSGIFQGDKVNIIGNGVVLDPALFK
AEAEALEASGHPLKERLHISKKAHLILPTHRILDAAYEAAKGDAKVGTTGKGIGPTYTDKVSRNGVRVGDILHNFEQKYG
AAKARHEQILKSLNYEYDLTELEKAWMEGIEYLKQFHFVDSEHEVNNYLKDGKSVLCEGAQGTMLDIDFGSYPFVTSSNT
VCAGACTGLGVAPNRIGEVFGIFKAYCTRVGAGPFPTELFDETGDKMCTLGHEFGSVTGRKRRCGWIDLVALKYSVMING
VTKLIMMKSDVLDTFDTIKACVAYKVDGEEIDYFPYDITEGVEPVYAELPGWKTDMTKMQSEDEFPEEFNAYLTFLEEQL
GVEIKIVSVGPDRAQTIERYTEE
>Mature_423_residues
MKVDVLLGLQWGDEGKGKVVDVLTPKYDVVARFQGGPNAGHTLEFEGQKYVLRSIPSGIFQGDKVNIIGNGVVLDPALFK
AEAEALEASGHPLKERLHISKKAHLILPTHRILDAAYEAAKGDAKVGTTGKGIGPTYTDKVSRNGVRVGDILHNFEQKYG
AAKARHEQILKSLNYEYDLTELEKAWMEGIEYLKQFHFVDSEHEVNNYLKDGKSVLCEGAQGTMLDIDFGSYPFVTSSNT
VCAGACTGLGVAPNRIGEVFGIFKAYCTRVGAGPFPTELFDETGDKMCTLGHEFGSVTGRKRRCGWIDLVALKYSVMING
VTKLIMMKSDVLDTFDTIKACVAYKVDGEEIDYFPYDITEGVEPVYAELPGWKTDMTKMQSEDEFPEEFNAYLTFLEEQL
GVEIKIVSVGPDRAQTIERYTEE

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=442, Percent_Identity=42.3076923076923, Blast_Score=342, Evalue=3e-94,
Organism=Homo sapiens, GI34577063, Length=436, Percent_Identity=42.8899082568807, Blast_Score=331, Evalue=8e-91,
Organism=Homo sapiens, GI40316944, Length=410, Percent_Identity=40.7317073170732, Blast_Score=299, Evalue=3e-81,
Organism=Escherichia coli, GI1790620, Length=428, Percent_Identity=46.2616822429907, Blast_Score=366, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI25146553, Length=431, Percent_Identity=45.0116009280742, Blast_Score=338, Evalue=3e-93,
Organism=Caenorhabditis elegans, GI25146555, Length=431, Percent_Identity=45.0116009280742, Blast_Score=337, Evalue=5e-93,
Organism=Saccharomyces cerevisiae, GI6324109, Length=430, Percent_Identity=45.5813953488372, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI21356033, Length=428, Percent_Identity=45.5607476635514, Blast_Score=355, Evalue=3e-98,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 46790; Mature: 46790

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVDVLLGLQWGDEGKGKVVDVLTPKYDVVARFQGGPNAGHTLEFEGQKYVLRSIPSGIF
CEEEEEEEEEECCCCCCCEEEEECCCEEEEEEECCCCCCCCEEEECCCHHHHHHCCHHHC
QGDKVNIIGNGVVLDPALFKAEAEALEASGHPLKERLHISKKAHLILPTHRILDAAYEAA
CCCEEEEEECCEEECHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEECHHHHHHHHHHHC
KGDAKVGTTGKGIGPTYTDKVSRNGVRVGDILHNFEQKYGAAKARHEQILKSLNYEYDLT
CCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHH
ELEKAWMEGIEYLKQFHFVDSEHEVNNYLKDGKSVLCEGAQGTMLDIDFGSYPFVTSSNT
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHEEECCCCCCEEEEECCCCCEEECCCC
VCAGACTGLGVAPNRIGEVFGIFKAYCTRVGAGPFPTELFDETGDKMCTLGHEFGSVTGR
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCEEECCHHHHCCCCC
KRRCGWIDLVALKYSVMINGVTKLIMMKSDVLDTFDTIKACVAYKVDGEEIDYFPYDITE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHC
GVEPVYAELPGWKTDMTKMQSEDEFPEEFNAYLTFLEEQLGVEIKIVSVGPDRAQTIERY
CCCHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHH
TEE
HCC
>Mature Secondary Structure
MKVDVLLGLQWGDEGKGKVVDVLTPKYDVVARFQGGPNAGHTLEFEGQKYVLRSIPSGIF
CEEEEEEEEEECCCCCCCEEEEECCCEEEEEEECCCCCCCCEEEECCCHHHHHHCCHHHC
QGDKVNIIGNGVVLDPALFKAEAEALEASGHPLKERLHISKKAHLILPTHRILDAAYEAA
CCCEEEEEECCEEECHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEECHHHHHHHHHHHC
KGDAKVGTTGKGIGPTYTDKVSRNGVRVGDILHNFEQKYGAAKARHEQILKSLNYEYDLT
CCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCHH
ELEKAWMEGIEYLKQFHFVDSEHEVNNYLKDGKSVLCEGAQGTMLDIDFGSYPFVTSSNT
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHEEECCCCCCEEEEECCCCCEEECCCC
VCAGACTGLGVAPNRIGEVFGIFKAYCTRVGAGPFPTELFDETGDKMCTLGHEFGSVTGR
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCEEECCHHHHCCCCC
KRRCGWIDLVALKYSVMINGVTKLIMMKSDVLDTFDTIKACVAYKVDGEEIDYFPYDITE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCHHC
GVEPVYAELPGWKTDMTKMQSEDEFPEEFNAYLTFLEEQLGVEIKIVSVGPDRAQTIERY
CCCHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHH
TEE
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA