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The map label for this gene is hisS [H]

Identifier: 29347250

GI number: 29347250

Start: 2311032

End: 2312396

Strand: Reverse

Name: hisS [H]

Synonym: BT_1840

Alternate gene names: 29347250

Gene position: 2312396-2311032 (Counterclockwise)

Preceding gene: 29347251

Following gene: 29347249

Centisome position: 36.94

GC content: 45.93

Gene sequence:

>1365_bases
ATGGCAGCAAAACCAAGTATTCCTAAAGGAACTCGTGACTTTTCGCCGGTAGAAATGGCGAAGCGTAATTATATATTCAA
TACGATTCGTGACGTATATCATCTGTATGGTTTCCAGCAGATAGAAACTCCGTCGATGGAGATGCTTTCTACACTGATGG
GGAAATATGGCGATGAGGGTGACAAACTTCTATTTAAGATTCAGAACTCCGGAGATTATTTTTCCGGCATCACGGATGAA
GAGTTATTGAGTCGTAATGCTGTTAAGCTGGCAAGCAAATTCTGTGAGAAAGGTTTGCGCTATGACTTGACAGTGCCTTT
TGCCCGTTACGTGGTGATGCACCGCGATGAAATAACTTTCCCGTTCAAGCGTTATCAGATACAGCCGGTATGGCGTGCAG
ACCGTCCGCAGAAAGGACGTTACCGTGAGTTCTATCAATGCGACGCTGATGTTGTCGGCAGCGATTCACTGCTGAATGAA
GTAGAACTGATGCAGATTGTCGATACAGTATTCAGCCGTTTCAACATCCGTGTGTGTATTAAGATCAATAACCGCAAGAT
TCTTTCCGGTATCGCTGAGATTATCGGTGAAGCGGATAAGATTGTAGATATCACAGTGGCTATTGACAAGCTGGATAAGA
TTGGTCTGGAGAACGTCAATGCGGAACTGAAAGAGAAAGGGATCAGTGATGAGGCGATTGCCAAATTGCAGCCGATCATT
CTGCTGAGCGGAACCAATACTGAAAAGCTGGCTACACTAAAGAGCGTGCTGGCAGCCAGTGAGACAGGCATGAAAGGAGT
CGAAGAAAGCGAATTTATTCTGGGCACATTGGAAACAATGGGCTTGAAAAATGAAATCGAACTTGACTTGACGCTGGCTC
GCGGATTGAATTATTATACCGGTGCTATCTTCGAAGTGAAAGCGCTGGATGTGCAGATCGGCAGTATCACCGGTGGCGGT
CGCTATGACAACCTGACAGGAGTATTCGGTATGGCCGGTGTGTCGGGAGTCGGCATTTCTTTTGGTGCAGACCGTATTTT
CGATGTATTGAATCAACTGGAACTTTATCCGAAGGAAGCAGTGAACGGTACGGAATTGATGTTCGTAAACTTTGGTGATA
AAGAAGCTGCTTTCTCCATGAGTATGCTGGCAAAAGTTCGTGCGGCAGGTATCCGTGCGGAAATCTTCCCGGATGCGGCA
AAGATGAAGAAGCAGATGAGCTATGCCAATGCCAAGAGTGTTCCGTTCGTAGCTATCGTAGGAGAGAACGAAATGAATGA
AGGCAAAGCAATGCTGAAGAATATGGAAACCGGCGAACAGAATCTTGTTTCGGTGGAAGAACTGATAGCTGCTTTGCGCA
ACTAA

Upstream 100 bases:

>100_bases
GCGTTCTTTTTTTATTGATGTTTTTTTTAGATATATGCAAGCGTTGCTCGGAAATTTGCAGTAAATTTGCGCAATTAAAA
TACTAAACAGTAATTATATC

Downstream 100 bases:

>100_bases
AAGCAGGGTATAGAGTAAGAATATCCGCTTTTCCGGTGAAGTACTTGACAAATGACTTTTTATGTTGTATCTTTGAGAAA
AGAAAAGGCATAAATAAGAT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MAAKPSIPKGTRDFSPVEMAKRNYIFNTIRDVYHLYGFQQIETPSMEMLSTLMGKYGDEGDKLLFKIQNSGDYFSGITDE
ELLSRNAVKLASKFCEKGLRYDLTVPFARYVVMHRDEITFPFKRYQIQPVWRADRPQKGRYREFYQCDADVVGSDSLLNE
VELMQIVDTVFSRFNIRVCIKINNRKILSGIAEIIGEADKIVDITVAIDKLDKIGLENVNAELKEKGISDEAIAKLQPII
LLSGTNTEKLATLKSVLAASETGMKGVEESEFILGTLETMGLKNEIELDLTLARGLNYYTGAIFEVKALDVQIGSITGGG
RYDNLTGVFGMAGVSGVGISFGADRIFDVLNQLELYPKEAVNGTELMFVNFGDKEAAFSMSMLAKVRAAGIRAEIFPDAA
KMKKQMSYANAKSVPFVAIVGENEMNEGKAMLKNMETGEQNLVSVEELIAALRN

Sequences:

>Translated_454_residues
MAAKPSIPKGTRDFSPVEMAKRNYIFNTIRDVYHLYGFQQIETPSMEMLSTLMGKYGDEGDKLLFKIQNSGDYFSGITDE
ELLSRNAVKLASKFCEKGLRYDLTVPFARYVVMHRDEITFPFKRYQIQPVWRADRPQKGRYREFYQCDADVVGSDSLLNE
VELMQIVDTVFSRFNIRVCIKINNRKILSGIAEIIGEADKIVDITVAIDKLDKIGLENVNAELKEKGISDEAIAKLQPII
LLSGTNTEKLATLKSVLAASETGMKGVEESEFILGTLETMGLKNEIELDLTLARGLNYYTGAIFEVKALDVQIGSITGGG
RYDNLTGVFGMAGVSGVGISFGADRIFDVLNQLELYPKEAVNGTELMFVNFGDKEAAFSMSMLAKVRAAGIRAEIFPDAA
KMKKQMSYANAKSVPFVAIVGENEMNEGKAMLKNMETGEQNLVSVEELIAALRN
>Mature_453_residues
AAKPSIPKGTRDFSPVEMAKRNYIFNTIRDVYHLYGFQQIETPSMEMLSTLMGKYGDEGDKLLFKIQNSGDYFSGITDEE
LLSRNAVKLASKFCEKGLRYDLTVPFARYVVMHRDEITFPFKRYQIQPVWRADRPQKGRYREFYQCDADVVGSDSLLNEV
ELMQIVDTVFSRFNIRVCIKINNRKILSGIAEIIGEADKIVDITVAIDKLDKIGLENVNAELKEKGISDEAIAKLQPIIL
LSGTNTEKLATLKSVLAASETGMKGVEESEFILGTLETMGLKNEIELDLTLARGLNYYTGAIFEVKALDVQIGSITGGGR
YDNLTGVFGMAGVSGVGISFGADRIFDVLNQLELYPKEAVNGTELMFVNFGDKEAAFSMSMLAKVRAAGIRAEIFPDAAK
MKKQMSYANAKSVPFVAIVGENEMNEGKAMLKNMETGEQNLVSVEELIAALRN

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=469, Percent_Identity=34.7547974413646, Blast_Score=248, Evalue=7e-66,
Organism=Homo sapiens, GI15029520, Length=469, Percent_Identity=31.7697228144989, Blast_Score=224, Evalue=2e-58,
Organism=Homo sapiens, GI65287717, Length=352, Percent_Identity=25.8522727272727, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1788861, Length=463, Percent_Identity=24.1900647948164, Blast_Score=100, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71993686, Length=481, Percent_Identity=35.5509355509356, Blast_Score=247, Evalue=9e-66,
Organism=Caenorhabditis elegans, GI71993693, Length=481, Percent_Identity=35.5509355509356, Blast_Score=247, Evalue=9e-66,
Organism=Saccharomyces cerevisiae, GI6325290, Length=472, Percent_Identity=34.5338983050847, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24643061, Length=488, Percent_Identity=33.8114754098361, Blast_Score=226, Evalue=4e-59,
Organism=Drosophila melanogaster, GI24643059, Length=506, Percent_Identity=32.6086956521739, Blast_Score=216, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 50398; Mature: 50267

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKPSIPKGTRDFSPVEMAKRNYIFNTIRDVYHLYGFQQIETPSMEMLSTLMGKYGDEG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHCCCCC
DKLLFKIQNSGDYFSGITDEELLSRNAVKLASKFCEKGLRYDLTVPFARYVVMHRDEITF
CEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHEECCCCCC
PFKRYQIQPVWRADRPQKGRYREFYQCDADVVGSDSLLNEVELMQIVDTVFSRFNIRVCI
CHHHEEEEECCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEE
KINNRKILSGIAEIIGEADKIVDITVAIDKLDKIGLENVNAELKEKGISDEAIAKLQPII
EECCCHHHHHHHHHHCCCCCEEEEEEEEHHHHHCCCCCCCHHHHHCCCCHHHHHHCCCEE
LLSGTNTEKLATLKSVLAASETGMKGVEESEFILGTLETMGLKNEIELDLTLARGLNYYT
EEECCCHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEHHHHCCCCCEEEEEEHHCCCCHHC
GAIFEVKALDVQIGSITGGGRYDNLTGVFGMAGVSGVGISFGADRIFDVLNQLELYPKEA
CCEEEEEEEEEEEECEECCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHC
VNGTELMFVNFGDKEAAFSMSMLAKVRAAGIRAEIFPDAAKMKKQMSYANAKSVPFVAIV
CCCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCCCCCCEEEEE
GENEMNEGKAMLKNMETGEQNLVSVEELIAALRN
CCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AAKPSIPKGTRDFSPVEMAKRNYIFNTIRDVYHLYGFQQIETPSMEMLSTLMGKYGDEG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHCCCCC
DKLLFKIQNSGDYFSGITDEELLSRNAVKLASKFCEKGLRYDLTVPFARYVVMHRDEITF
CEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHEECCCCCC
PFKRYQIQPVWRADRPQKGRYREFYQCDADVVGSDSLLNEVELMQIVDTVFSRFNIRVCI
CHHHEEEEECCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEE
KINNRKILSGIAEIIGEADKIVDITVAIDKLDKIGLENVNAELKEKGISDEAIAKLQPII
EECCCHHHHHHHHHHCCCCCEEEEEEEEHHHHHCCCCCCCHHHHHCCCCHHHHHHCCCEE
LLSGTNTEKLATLKSVLAASETGMKGVEESEFILGTLETMGLKNEIELDLTLARGLNYYT
EEECCCHHHHHHHHHHHHHHHCCCCCCCCCHHHEEEHHHHCCCCCEEEEEEHHCCCCHHC
GAIFEVKALDVQIGSITGGGRYDNLTGVFGMAGVSGVGISFGADRIFDVLNQLELYPKEA
CCEEEEEEEEEEEECEECCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHC
VNGTELMFVNFGDKEAAFSMSMLAKVRAAGIRAEIFPDAAKMKKQMSYANAKSVPFVAIV
CCCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCCCCCCEEEEE
GENEMNEGKAMLKNMETGEQNLVSVEELIAALRN
CCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA