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The map label for this gene is groEL [H]

Identifier: 29347239

GI number: 29347239

Start: 2293913

End: 2295550

Strand: Reverse

Name: groEL [H]

Synonym: BT_1829

Alternate gene names: 29347239

Gene position: 2295550-2293913 (Counterclockwise)

Preceding gene: 29347240

Following gene: 29347238

Centisome position: 36.67

GC content: 45.85

Gene sequence:

>1638_bases
ATGGCAAAAGAAATATTATTCAATATCGATGCCCGCGACCAATTGAAAAAAGGTGTCGATGCATTGGCAAATGCAGTAAA
AGTAACTCTTGGCCCGAAAGGACGTAACGTTATCATCGAAAAGAAATTCGGTGCTCCGCACATCACAAAAGACGGGGTGA
CAGTAGCAAAGGAGATCGAATTGGCAGACGCTTACCAAAATACCGGTGCACAACTGGTGAAAGAAGTCGCTTCTAAGACT
GGTGACGATGCAGGTGACGGTACAACTACTGCAACTGTTTTGGCTCAGGCTATCGTAGCTGAAGGTTTGAAGAATGTGAC
TGCCGGTGCAAGCCCGATGGATATCAAACGTGGTATTGACAAGGCTGTTGCCAAAGTGGTAGAATCAATCAAGGCACAAG
CTGAAACTGTAGGTGACAACTACGACAAGATCGAACAGGTTGCTACTGTATCTGCTAACAATGATCCGGTAATCGGTAAA
TTGATTGCTGACGCTATGCGCAAAGTTTCTAAGGACGGTGTAATCACTATCGAAGAAGCTAAAGGTACTGACACTACTAT
CGGTGTAGTAGAAGGTATGCAGTTCGACCGCGGTTATCTGTCTGCTTATTTCGTTACTAATACGGAAAAGATGGAATGTG
AGATGGAGAAACCTTACATTCTGATCTACGACAAGAAGATTTCTAATCTGAAAGATTTCTTGCCTATCCTCGAACCGGCT
GTTCAGACAGGTCGTCCTCTGTTGGTTATCGCAGAAGATGTGGATAGCGAAGCGTTGACTACATTGGTAGTAAACCGTCT
GCGTTCTCAGCTGAAGATTTGTGCAGTGAAGGCTCCGGGCTTCGGCGACCGTCGTAAAGAAATGCTGGAAGATATCGCTA
TCCTGACAGGTGGTGTGGTAATCAGCGAGGAAAAAGGTCTGAAACTGGAACAGGCTACAATCGAAATGCTCGGTACTGCT
GACAAGGTAACAGTATCTAAAGATTATACAACAATCGTAAACGGTGCTGGTGTGAAAGAAAACATCAAGGAACGTTGCGA
TCAGATCAAGGCTCAGATCGTTGCTACTAAATCAGATTACGACCGTGAGAAATTGCAGGAACGTCTGGCTAAATTGTCAG
GTGGTGTAGCAGTTCTCTATGTAGGTGCTGCTTCTGAAGTAGAAATGAAAGAAAAGAAAGACCGTGTAGACGATGCATTG
CGTGCAACTCGCGCTGCTATCGAAGAAGGTATCATCCCGGGTGGTGGCGTTGCTTACATCCGTGCTATCGATTCTCTTGA
AGGCATGAAGGGGGACAATGCGGATGAAACAACAGGTATCGGTATCATCAAACGTGCTATCGAAGAACCGCTTCGCGAAA
TTGTTGCCAATGCAGGCAAGGAAGGTGCGGTAGTTGTTCAGAAAGTACGTGAAGGTAAAGGTGACTTCGGTTACAATGCT
CGTACGGATGTTTACGAAAATCTGCATGCTGCCGGTGTAGTAGATCCTGCTAAAGTAGCTCGTGTGGCTCTTGAAAATGC
AGCTTCTATCGCAGGTATGTTCCTGACTACAGAATGTGTGATCGTGGAAAAGAAAGAAGATAAACCAGAAATGCCGATGG
GCGCTCCGGGAATGGGCGGCATGGGCGGAATGATGTAA

Upstream 100 bases:

>100_bases
AAGGCACTAAATACCTTATCATGCGTCAGAGCGATGTTCTCGCTATCTTGGGTTAATTCAAAATTCTAAATTTATCATAT
TAAATAAATAAGATATCATT

Downstream 100 bases:

>100_bases
TTCTTCCTTTCCGGACATAAAAATAAGGTATGGAACCGCTTTCCCTGTGGGGGAGGCGGTTCTTTTTTTGCGCAGCTTTG
TGATAGAAACTTTTGTGTGT

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60 [H]

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MAKEILFNIDARDQLKKGVDALANAVKVTLGPKGRNVIIEKKFGAPHITKDGVTVAKEIELADAYQNTGAQLVKEVASKT
GDDAGDGTTTATVLAQAIVAEGLKNVTAGASPMDIKRGIDKAVAKVVESIKAQAETVGDNYDKIEQVATVSANNDPVIGK
LIADAMRKVSKDGVITIEEAKGTDTTIGVVEGMQFDRGYLSAYFVTNTEKMECEMEKPYILIYDKKISNLKDFLPILEPA
VQTGRPLLVIAEDVDSEALTTLVVNRLRSQLKICAVKAPGFGDRRKEMLEDIAILTGGVVISEEKGLKLEQATIEMLGTA
DKVTVSKDYTTIVNGAGVKENIKERCDQIKAQIVATKSDYDREKLQERLAKLSGGVAVLYVGAASEVEMKEKKDRVDDAL
RATRAAIEEGIIPGGGVAYIRAIDSLEGMKGDNADETTGIGIIKRAIEEPLREIVANAGKEGAVVVQKVREGKGDFGYNA
RTDVYENLHAAGVVDPAKVARVALENAASIAGMFLTTECVIVEKKEDKPEMPMGAPGMGGMGGMM

Sequences:

>Translated_545_residues
MAKEILFNIDARDQLKKGVDALANAVKVTLGPKGRNVIIEKKFGAPHITKDGVTVAKEIELADAYQNTGAQLVKEVASKT
GDDAGDGTTTATVLAQAIVAEGLKNVTAGASPMDIKRGIDKAVAKVVESIKAQAETVGDNYDKIEQVATVSANNDPVIGK
LIADAMRKVSKDGVITIEEAKGTDTTIGVVEGMQFDRGYLSAYFVTNTEKMECEMEKPYILIYDKKISNLKDFLPILEPA
VQTGRPLLVIAEDVDSEALTTLVVNRLRSQLKICAVKAPGFGDRRKEMLEDIAILTGGVVISEEKGLKLEQATIEMLGTA
DKVTVSKDYTTIVNGAGVKENIKERCDQIKAQIVATKSDYDREKLQERLAKLSGGVAVLYVGAASEVEMKEKKDRVDDAL
RATRAAIEEGIIPGGGVAYIRAIDSLEGMKGDNADETTGIGIIKRAIEEPLREIVANAGKEGAVVVQKVREGKGDFGYNA
RTDVYENLHAAGVVDPAKVARVALENAASIAGMFLTTECVIVEKKEDKPEMPMGAPGMGGMGGMM
>Mature_544_residues
AKEILFNIDARDQLKKGVDALANAVKVTLGPKGRNVIIEKKFGAPHITKDGVTVAKEIELADAYQNTGAQLVKEVASKTG
DDAGDGTTTATVLAQAIVAEGLKNVTAGASPMDIKRGIDKAVAKVVESIKAQAETVGDNYDKIEQVATVSANNDPVIGKL
IADAMRKVSKDGVITIEEAKGTDTTIGVVEGMQFDRGYLSAYFVTNTEKMECEMEKPYILIYDKKISNLKDFLPILEPAV
QTGRPLLVIAEDVDSEALTTLVVNRLRSQLKICAVKAPGFGDRRKEMLEDIAILTGGVVISEEKGLKLEQATIEMLGTAD
KVTVSKDYTTIVNGAGVKENIKERCDQIKAQIVATKSDYDREKLQERLAKLSGGVAVLYVGAASEVEMKEKKDRVDDALR
ATRAAIEEGIIPGGGVAYIRAIDSLEGMKGDNADETTGIGIIKRAIEEPLREIVANAGKEGAVVVQKVREGKGDFGYNAR
TDVYENLHAAGVVDPAKVARVALENAASIAGMFLTTECVIVEKKEDKPEMPMGAPGMGGMGGMM

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family [H]

Homologues:

Organism=Homo sapiens, GI41399285, Length=531, Percent_Identity=49.1525423728814, Blast_Score=493, Evalue=1e-139,
Organism=Homo sapiens, GI31542947, Length=531, Percent_Identity=49.1525423728814, Blast_Score=493, Evalue=1e-139,
Organism=Escherichia coli, GI1790586, Length=522, Percent_Identity=59.5785440613027, Blast_Score=613, Evalue=1e-177,
Organism=Caenorhabditis elegans, GI17555558, Length=524, Percent_Identity=48.8549618320611, Blast_Score=495, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI193210679, Length=234, Percent_Identity=43.5897435897436, Blast_Score=190, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6323288, Length=525, Percent_Identity=54.2857142857143, Blast_Score=549, Evalue=1e-157,
Organism=Saccharomyces cerevisiae, GI6322524, Length=124, Percent_Identity=29.0322580645161, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24641193, Length=528, Percent_Identity=50.3787878787879, Blast_Score=513, Evalue=1e-145,
Organism=Drosophila melanogaster, GI24641191, Length=528, Percent_Identity=50.3787878787879, Blast_Score=513, Evalue=1e-145,
Organism=Drosophila melanogaster, GI45550936, Length=523, Percent_Identity=48.7571701720841, Blast_Score=491, Evalue=1e-139,
Organism=Drosophila melanogaster, GI45550132, Length=523, Percent_Identity=48.7571701720841, Blast_Score=491, Evalue=1e-139,
Organism=Drosophila melanogaster, GI45550935, Length=523, Percent_Identity=48.7571701720841, Blast_Score=491, Evalue=1e-139,
Organism=Drosophila melanogaster, GI17864606, Length=542, Percent_Identity=42.9889298892989, Blast_Score=432, Evalue=1e-121,
Organism=Drosophila melanogaster, GI24584129, Length=535, Percent_Identity=36.8224299065421, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI19921262, Length=535, Percent_Identity=36.8224299065421, Blast_Score=320, Evalue=1e-87,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 58204; Mature: 58072

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEILFNIDARDQLKKGVDALANAVKVTLGPKGRNVIIEKKFGAPHITKDGVTVAKEIE
CCCCEEEECCCHHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHH
LADAYQNTGAQLVKEVASKTGDDAGDGTTTATVLAQAIVAEGLKNVTAGASPMDIKRGID
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
KAVAKVVESIKAQAETVGDNYDKIEQVATVSANNDPVIGKLIADAMRKVSKDGVITIEEA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCEEEEECC
KGTDTTIGVVEGMQFDRGYLSAYFVTNTEKMECEMEKPYILIYDKKISNLKDFLPILEPA
CCCCCEEEHHCCCEECCCCEEEEEEECCHHHEEECCCCEEEEECCHHHHHHHHHHHHHHH
VQTGRPLLVIAEDVDSEALTTLVVNRLRSQLKICAVKAPGFGDRRKEMLEDIAILTGGVV
HHCCCCEEEEECCCCHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHCCEE
ISEEKGLKLEQATIEMLGTADKVTVSKDYTTIVNGAGVKENIKERCDQIKAQIVATKSDY
EECCCCCCHHHHHHHHHCCCCCEEECCCCCEEECCCCCHHHHHHHHHHHHHHEEECCCCC
DREKLQERLAKLSGGVAVLYVGAASEVEMKEKKDRVDDALRATRAAIEEGIIPGGGVAYI
CHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
RAIDSLEGMKGDNADETTGIGIIKRAIEEPLREIVANAGKEGAVVVQKVREGKGDFGYNA
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCC
RTDVYENLHAAGVVDPAKVARVALENAASIAGMFLTTECVIVEKKEDKPEMPMGAPGMGG
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCCCCCCCCC
MGGMM
CCCCC
>Mature Secondary Structure 
AKEILFNIDARDQLKKGVDALANAVKVTLGPKGRNVIIEKKFGAPHITKDGVTVAKEIE
CCCEEEECCCHHHHHHHHHHHHHHEEEEECCCCCEEEEEECCCCCCCCCCCCHHHHHHH
LADAYQNTGAQLVKEVASKTGDDAGDGTTTATVLAQAIVAEGLKNVTAGASPMDIKRGID
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
KAVAKVVESIKAQAETVGDNYDKIEQVATVSANNDPVIGKLIADAMRKVSKDGVITIEEA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCCCCHHHHHHHHHHHHHCCCCEEEEECC
KGTDTTIGVVEGMQFDRGYLSAYFVTNTEKMECEMEKPYILIYDKKISNLKDFLPILEPA
CCCCCEEEHHCCCEECCCCEEEEEEECCHHHEEECCCCEEEEECCHHHHHHHHHHHHHHH
VQTGRPLLVIAEDVDSEALTTLVVNRLRSQLKICAVKAPGFGDRRKEMLEDIAILTGGVV
HHCCCCEEEEECCCCHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHCCEE
ISEEKGLKLEQATIEMLGTADKVTVSKDYTTIVNGAGVKENIKERCDQIKAQIVATKSDY
EECCCCCCHHHHHHHHHCCCCCEEECCCCCEEECCCCCHHHHHHHHHHHHHHEEECCCCC
DREKLQERLAKLSGGVAVLYVGAASEVEMKEKKDRVDDALRATRAAIEEGIIPGGGVAYI
CHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
RAIDSLEGMKGDNADETTGIGIIKRAIEEPLREIVANAGKEGAVVVQKVREGKGDFGYNA
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCC
RTDVYENLHAAGVVDPAKVARVALENAASIAGMFLTTECVIVEKKEDKPEMPMGAPGMGG
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCCCCCCCCC
MGGMM
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA