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The map label for this gene is eptA [H]

Identifier: 29347223

GI number: 29347223

Start: 2274992

End: 2276695

Strand: Reverse

Name: eptA [H]

Synonym: BT_1813

Alternate gene names: 29347223

Gene position: 2276695-2274992 (Counterclockwise)

Preceding gene: 29347224

Following gene: 29347222

Centisome position: 36.37

GC content: 45.13

Gene sequence:

>1704_bases
ATGAAGCTTTTTAAGAACATAAAGAAATGGTTTGAAAATCAGGAACACTTGTTTTACCTGTTCCTGATCATCCTGATAAT
GCCCAACATCGTTCTGGGTTTCACAGAACCCTTACCGTTGATGGCGAAGGTAAGCAATGTCTTGTTGCCTTTAGCCTGTT
ACTATCTGCTGATGACTTTGTCCCGCAACTGTGGCAAGATGCTATGGATTTTGTTCATCTTCCTGTTTTTCGGCGCCTTT
CAGATTGTGCTGCTTTATCTGTTCGGACAGTCCATCATCGCTGTAGATATGTTTCTAAATCTGGTAACCACCAATTCGAG
TGAAGCGATGGAGTTGCTGGACAACTTATTGCCGGCCATAGTCACCGTAGTCATACTTTACATTCCTGCCCTGATATTGG
GCATGATTTCAATTATCCGCAAGCGCCGGCTTTCTGTGGCATTTATTCGCAGAGAACGGAAGAGAGCGTTCATCCTGCTT
GGAATCTCTCTTCTCGCCCTGGGCGGAGCTTACTGGGAAGACTCCCGCTATGAACTGAAATCCGATCTTTATCCTCTCAA
TGTATGCTATAATGTGGGACTTGCTTTTCAACGGACGGCATTGACGCAAAATTACCATCATACCTCGAAAGATTTTACTT
TCCATGCCCAAGCCACTCATCCGGTGGACAAGCGTGAAGTGTATGTAATGGTAGTGGGAGAGACTTCCCGCGCATTGAAC
TGGCAACTCTACGGATATGAGCGGGAGACGAATCCGCTTCTGTCCCGTCAACCGGGAATTGTTGCTTTCTCCAAAGTCCT
GACGGAGTCGAATACCACCCATAAAAGCGTACCCATGCTGATGTCCGATATTACAGCCTGTAACTTCGACTCCATTTACC
ATCAAAAAGGAATCATCACGGCCTTTAAGGAGGCAGGTTATCAGACAGCCTTTTTCTCCAACCAGCGCTATAATCATTCC
TTTATCGATTTCTTCGGAAAGGAAGCCGATACATTCGATTTTATAAAAGAAGATTCGCTGGATTCCGCCTACAATCCTTC
TGATGATGAACTGCTGGCATTAGTAGCGAAAGAATTATCCAAGGGAAATCAGAAACAGTTTATCGTACTTCACACATACG
GCTCACATTTCAATTATCGTGAACGTTATCCGTCGGAAGACGCTTTCTTTCTGCCCGATTATCCGGTAGAAGCGGAAGTG
AAGTACCGTGATAACCTGGTGAATGCTTACGATAACACCATCCGCTATACAGATAGCTTTCTGTCACGTCTGATTCATAT
GCTCGAAGAACAGCATGTGGATGCTGCAATGCTCTACACCTCCGATCATGGAGAGGATATTTTTGACGATTCCCGTCATC
TGTTCCTTCATGCTTCTCCGGTTCCTTCTTACTATCAGCTTCATGTTCCGTTTCTGATATGGATGTCCGACACCTATCGG
GAAGCTTATCCCGAACATTGGCAAACCGTGACCAAGAATAAGGATAAGGACCTATCTTCCAGCCGTTCATTCTTTCCTAC
CATGCTGGAACTGGGGGGAGTGCAAACTTCTTACCGCAACGATTCCTCATCTGTAGTCAGCCCTCTTTACACCATGAAGC
CGAGAGTATATCTGAATGATCATAACGAACCACGCCCCTTGGATGATCTGGGAATGAAACAGCCTGACTTCCAGAAATGT
CAAGTATTAGGTATTAAGTATTAG

Upstream 100 bases:

>100_bases
AATTATGGTTTCGGCTATCGCTGGGAATTTAAGAAGAGAGTAAATGTACGTTTGGATTTAGGGTTTGGCAAGCACCAGAC
CGGATTTATATTTAATATCA

Downstream 100 bases:

>100_bases
GTAATAGGTAATAAGTAATAGGTAATAAGTAATACACCGTAGCCGTACAGGCAAGGCTATACGAAGCTACGATTATTACC
TATTACTTATTACCTATTAC

Product: putative integral membrane protein

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 567; Mature: 567

Protein sequence:

>567_residues
MKLFKNIKKWFENQEHLFYLFLIILIMPNIVLGFTEPLPLMAKVSNVLLPLACYYLLMTLSRNCGKMLWILFIFLFFGAF
QIVLLYLFGQSIIAVDMFLNLVTTNSSEAMELLDNLLPAIVTVVILYIPALILGMISIIRKRRLSVAFIRRERKRAFILL
GISLLALGGAYWEDSRYELKSDLYPLNVCYNVGLAFQRTALTQNYHHTSKDFTFHAQATHPVDKREVYVMVVGETSRALN
WQLYGYERETNPLLSRQPGIVAFSKVLTESNTTHKSVPMLMSDITACNFDSIYHQKGIITAFKEAGYQTAFFSNQRYNHS
FIDFFGKEADTFDFIKEDSLDSAYNPSDDELLALVAKELSKGNQKQFIVLHTYGSHFNYRERYPSEDAFFLPDYPVEAEV
KYRDNLVNAYDNTIRYTDSFLSRLIHMLEEQHVDAAMLYTSDHGEDIFDDSRHLFLHASPVPSYYQLHVPFLIWMSDTYR
EAYPEHWQTVTKNKDKDLSSSRSFFPTMLELGGVQTSYRNDSSSVVSPLYTMKPRVYLNDHNEPRPLDDLGMKQPDFQKC
QVLGIKY

Sequences:

>Translated_567_residues
MKLFKNIKKWFENQEHLFYLFLIILIMPNIVLGFTEPLPLMAKVSNVLLPLACYYLLMTLSRNCGKMLWILFIFLFFGAF
QIVLLYLFGQSIIAVDMFLNLVTTNSSEAMELLDNLLPAIVTVVILYIPALILGMISIIRKRRLSVAFIRRERKRAFILL
GISLLALGGAYWEDSRYELKSDLYPLNVCYNVGLAFQRTALTQNYHHTSKDFTFHAQATHPVDKREVYVMVVGETSRALN
WQLYGYERETNPLLSRQPGIVAFSKVLTESNTTHKSVPMLMSDITACNFDSIYHQKGIITAFKEAGYQTAFFSNQRYNHS
FIDFFGKEADTFDFIKEDSLDSAYNPSDDELLALVAKELSKGNQKQFIVLHTYGSHFNYRERYPSEDAFFLPDYPVEAEV
KYRDNLVNAYDNTIRYTDSFLSRLIHMLEEQHVDAAMLYTSDHGEDIFDDSRHLFLHASPVPSYYQLHVPFLIWMSDTYR
EAYPEHWQTVTKNKDKDLSSSRSFFPTMLELGGVQTSYRNDSSSVVSPLYTMKPRVYLNDHNEPRPLDDLGMKQPDFQKC
QVLGIKY
>Mature_567_residues
MKLFKNIKKWFENQEHLFYLFLIILIMPNIVLGFTEPLPLMAKVSNVLLPLACYYLLMTLSRNCGKMLWILFIFLFFGAF
QIVLLYLFGQSIIAVDMFLNLVTTNSSEAMELLDNLLPAIVTVVILYIPALILGMISIIRKRRLSVAFIRRERKRAFILL
GISLLALGGAYWEDSRYELKSDLYPLNVCYNVGLAFQRTALTQNYHHTSKDFTFHAQATHPVDKREVYVMVVGETSRALN
WQLYGYERETNPLLSRQPGIVAFSKVLTESNTTHKSVPMLMSDITACNFDSIYHQKGIITAFKEAGYQTAFFSNQRYNHS
FIDFFGKEADTFDFIKEDSLDSAYNPSDDELLALVAKELSKGNQKQFIVLHTYGSHFNYRERYPSEDAFFLPDYPVEAEV
KYRDNLVNAYDNTIRYTDSFLSRLIHMLEEQHVDAAMLYTSDHGEDIFDDSRHLFLHASPVPSYYQLHVPFLIWMSDTYR
EAYPEHWQTVTKNKDKDLSSSRSFFPTMLELGGVQTSYRNDSSSVVSPLYTMKPRVYLNDHNEPRPLDDLGMKQPDFQKC
QVLGIKY

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=304, Percent_Identity=29.9342105263158, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI87082286, Length=303, Percent_Identity=29.7029702970297, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI87082223, Length=254, Percent_Identity=29.9212598425197, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1790392, Length=322, Percent_Identity=25.1552795031056, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787035, Length=489, Percent_Identity=25.3578732106339, Blast_Score=93, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 65736; Mature: 65736

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFKNIKKWFENQEHLFYLFLIILIMPNIVLGFTEPLPLMAKVSNVLLPLACYYLLMTL
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SRNCGKMLWILFIFLFFGAFQIVLLYLFGQSIIAVDMFLNLVTTNSSEAMELLDNLLPAI
HCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VTVVILYIPALILGMISIIRKRRLSVAFIRRERKRAFILLGISLLALGGAYWEDSRYELK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHCCCCCCCCCHHHH
SDLYPLNVCYNVGLAFQRTALTQNYHHTSKDFTFHAQATHPVDKREVYVMVVGETSRALN
HCCCEEHHHHHCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCEEE
WQLYGYERETNPLLSRQPGIVAFSKVLTESNTTHKSVPMLMSDITACNFDSIYHQKGIIT
EEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHCCHHH
AFKEAGYQTAFFSNQRYNHSFIDFFGKEADTFDFIKEDSLDSAYNPSDDELLALVAKELS
HHHHCCCEEEEECCCCCCCHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHC
KGNQKQFIVLHTYGSHFNYRERYPSEDAFFLPDYPVEAEVKYRDNLVNAYDNTIRYTDSF
CCCCCEEEEEEECCCCCCHHHCCCCCCCEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHH
LSRLIHMLEEQHVDAAMLYTSDHGEDIFDDSRHLFLHASPVPSYYQLHVPFLIWMSDTYR
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCEEEECCEEEEECCHHH
EAYPEHWQTVTKNKDKDLSSSRSFFPTMLELGGVQTSYRNDSSSVVSPLYTMKPRVYLND
HHCHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEECC
HNEPRPLDDLGMKQPDFQKCQVLGIKY
CCCCCCHHHCCCCCCCCCHHEEEECCC
>Mature Secondary Structure
MKLFKNIKKWFENQEHLFYLFLIILIMPNIVLGFTEPLPLMAKVSNVLLPLACYYLLMTL
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SRNCGKMLWILFIFLFFGAFQIVLLYLFGQSIIAVDMFLNLVTTNSSEAMELLDNLLPAI
HCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VTVVILYIPALILGMISIIRKRRLSVAFIRRERKRAFILLGISLLALGGAYWEDSRYELK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHCCCCCCCCCHHHH
SDLYPLNVCYNVGLAFQRTALTQNYHHTSKDFTFHAQATHPVDKREVYVMVVGETSRALN
HCCCEEHHHHHCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCEEE
WQLYGYERETNPLLSRQPGIVAFSKVLTESNTTHKSVPMLMSDITACNFDSIYHQKGIIT
EEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHCCHHH
AFKEAGYQTAFFSNQRYNHSFIDFFGKEADTFDFIKEDSLDSAYNPSDDELLALVAKELS
HHHHCCCEEEEECCCCCCCHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHC
KGNQKQFIVLHTYGSHFNYRERYPSEDAFFLPDYPVEAEVKYRDNLVNAYDNTIRYTDSF
CCCCCEEEEEEECCCCCCHHHCCCCCCCEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHH
LSRLIHMLEEQHVDAAMLYTSDHGEDIFDDSRHLFLHASPVPSYYQLHVPFLIWMSDTYR
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCEEEECCEEEEECCHHH
EAYPEHWQTVTKNKDKDLSSSRSFFPTMLELGGVQTSYRNDSSSVVSPLYTMKPRVYLND
HHCHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEECC
HNEPRPLDDLGMKQPDFQKCQVLGIKY
CCCCCCHHHCCCCCCCCCHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609 [H]