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The map label for this gene is sacC [H]

Identifier: 29347169

GI number: 29347169

Start: 2173001

End: 2174833

Strand: Reverse

Name: sacC [H]

Synonym: BT_1759

Alternate gene names: 29347169

Gene position: 2174833-2173001 (Counterclockwise)

Preceding gene: 29347170

Following gene: 29347168

Centisome position: 34.74

GC content: 46.75

Gene sequence:

>1833_bases
ATGAATGTATTTTTGCCTGTCAAACAACTTCAGACCTTTCTGATTTGTTTTATTCTCATGACTATTTCTGTTTCAACCCG
AGCGGCGGATTCTCCTTTGCTGATTAAGAATCTGGGAGAAGGTCATTGCCTTGTACGTGTCAATACTTCCCAGAACTACC
TGCTTCTGCCCGTAGAGGATGCGTCTCCCGATGTACGTATCAGCATGATCGTTAATAATAAAGAGGTGAAAAACTTCGAC
GTGCGTCTCGCCATACATAAAGTAGATTACTTTGTTCCGGTAGACCTTTCCGACTTTTCCGGCAAAACGGTCTCTTTCAA
GTTCAAGATGAACTCCAACGATCCGATCCGTGTCAATCTTTCGCCGGATAATACCGCTTGCTGCAAGGAAATGAAACTCT
CCGACACCTTCGATACGACCAACCGTGAAAAGTTTCGTCCGACCTATCACTTCTCTCCCCTTTACGGATGGATGAACGAC
CCGAACGGAATGGTCTATAAAGACGGTGAATACCATCTTTTTTACCAGTACAACCCTTATGGCTCCAAATGGGGAAACAT
GAACTGGGGACACGCTATCAGCAAAGACCTCGTAAACTGGGAACACCGCCCCGTTGCGATAGCACCGGATGCGCTTGGTA
CTATCTTCAGCGGCTCTGCCGTGGTAGATCATAACAATACAGCCGGCTTCGGAGCAGGCGCAATCATCGCCATCTATACA
CAAAACAGCGACCGTCAGGTACAAAGCATCGCTTATAGCACTGACAACGGACGCACTTTCACCAAATACGAAAACAACCC
CGTACTTGTATCCGAAGCTCGTGATTTCCGCGACCCGAAAGTTTTTTGGTACGAAGCAACAAAACGCTGGATCATGGTGC
TCGCAGTGGGACAGGAAATGCAGATTTTCTCTTCTCCCAATTTGAAAGACTGGGCATTTGAAAGCAGCTTCGGCGAAGGA
TACGGTGCTCACGGCAACGTATGGGAATGCCCCGATTTATTCGAACTTCCGGTAGAGGGCACCAACGAAAAGAAATGGGT
ATTGCTTTGCAGCCTCGGTGACGGTCCTTTCGGTGACTCCGCTACTCAATACTTTATCGGTAGCTTCGACGGCAAGAAAT
TTAGTTGTGACAATCAGCCGAATGTCACGAAATGGATGGACTGGGGAAAAGACCATTATGCTACAGTTACTTGGAGCGAT
GCCCCCGACAACCGCCGTATCGCCATTGCATGGATGAGCAACTGGCAATATGCAAATGACGTGCCAACTTCTCAATACCG
TAGTCCGAACTCCATCCCTCGTGATTTGAGTCTCTTTGCCATCGACGGTGGGATTTATCTTCAATCCGCTCCTTCTCCCG
AATTACTGAAACTGCGTGGTGTTTCCAAAAAGCGCTCATTCAAGGTTAACGGAACACGTATCGTGAAAGATTTGATTCCG
AACAATGAAGGTGCTTACGAAATAGAACTGTCCCTTAAGAATCAGCAGGCAGAAATCATCGGTTTCCGTCTCTACAATGA
CAAGGGCGAAGAAGTAGACATGCAGTACGATATGAAAGAGAAGAAATTCTCGATGGACCGTCGCAAGAGTGGCGAGGTAA
ATTTCAACGAAAACTTCCCGATGCTGACTTGGACTGCGATTGAAGAGGATAAGGATGAAATGAAACTCCGCCTGTTTGTA
GACAGATCGAGTGTAGAGGCTTTCGGTGACGGTGGACGCTTCGCTATGACCAATCAGGTATTCCCTTCGGAACCTTATAA
TCATATCAGCTTCTACAGCAAAGGCGGTGCCTATAAAGTAGATTCATTCGTGGTTTACAAACTGAAGAAATAA

Upstream 100 bases:

>100_bases
TACAGCGTTTGAAACAAAAGTGATAGGCGCTAACCTTGGAAGCGCCTATCTTTGCACTGTAACAACTATCAAAAGTATAA
CAACCTTAATACCATAGAAC

Downstream 100 bases:

>100_bases
AAAACGACTTTCTTCTCCCTGCGCTCTTTTTAATTCATGCTAACATACCTTTATCCTTGCGTTAACATGCCTTTATGTTC
TCCTTATGTATGGGAGATAG

Product: 2,6-beta-D-fructofuranosidase

Products: NA

Alternate protein names: Beta-D-fructofuranosidase; Exo-beta-D-fructosidase; Exo-levanase [H]

Number of amino acids: Translated: 610; Mature: 610

Protein sequence:

>610_residues
MNVFLPVKQLQTFLICFILMTISVSTRAADSPLLIKNLGEGHCLVRVNTSQNYLLLPVEDASPDVRISMIVNNKEVKNFD
VRLAIHKVDYFVPVDLSDFSGKTVSFKFKMNSNDPIRVNLSPDNTACCKEMKLSDTFDTTNREKFRPTYHFSPLYGWMND
PNGMVYKDGEYHLFYQYNPYGSKWGNMNWGHAISKDLVNWEHRPVAIAPDALGTIFSGSAVVDHNNTAGFGAGAIIAIYT
QNSDRQVQSIAYSTDNGRTFTKYENNPVLVSEARDFRDPKVFWYEATKRWIMVLAVGQEMQIFSSPNLKDWAFESSFGEG
YGAHGNVWECPDLFELPVEGTNEKKWVLLCSLGDGPFGDSATQYFIGSFDGKKFSCDNQPNVTKWMDWGKDHYATVTWSD
APDNRRIAIAWMSNWQYANDVPTSQYRSPNSIPRDLSLFAIDGGIYLQSAPSPELLKLRGVSKKRSFKVNGTRIVKDLIP
NNEGAYEIELSLKNQQAEIIGFRLYNDKGEEVDMQYDMKEKKFSMDRRKSGEVNFNENFPMLTWTAIEEDKDEMKLRLFV
DRSSVEAFGDGGRFAMTNQVFPSEPYNHISFYSKGGAYKVDSFVVYKLKK

Sequences:

>Translated_610_residues
MNVFLPVKQLQTFLICFILMTISVSTRAADSPLLIKNLGEGHCLVRVNTSQNYLLLPVEDASPDVRISMIVNNKEVKNFD
VRLAIHKVDYFVPVDLSDFSGKTVSFKFKMNSNDPIRVNLSPDNTACCKEMKLSDTFDTTNREKFRPTYHFSPLYGWMND
PNGMVYKDGEYHLFYQYNPYGSKWGNMNWGHAISKDLVNWEHRPVAIAPDALGTIFSGSAVVDHNNTAGFGAGAIIAIYT
QNSDRQVQSIAYSTDNGRTFTKYENNPVLVSEARDFRDPKVFWYEATKRWIMVLAVGQEMQIFSSPNLKDWAFESSFGEG
YGAHGNVWECPDLFELPVEGTNEKKWVLLCSLGDGPFGDSATQYFIGSFDGKKFSCDNQPNVTKWMDWGKDHYATVTWSD
APDNRRIAIAWMSNWQYANDVPTSQYRSPNSIPRDLSLFAIDGGIYLQSAPSPELLKLRGVSKKRSFKVNGTRIVKDLIP
NNEGAYEIELSLKNQQAEIIGFRLYNDKGEEVDMQYDMKEKKFSMDRRKSGEVNFNENFPMLTWTAIEEDKDEMKLRLFV
DRSSVEAFGDGGRFAMTNQVFPSEPYNHISFYSKGGAYKVDSFVVYKLKK
>Mature_610_residues
MNVFLPVKQLQTFLICFILMTISVSTRAADSPLLIKNLGEGHCLVRVNTSQNYLLLPVEDASPDVRISMIVNNKEVKNFD
VRLAIHKVDYFVPVDLSDFSGKTVSFKFKMNSNDPIRVNLSPDNTACCKEMKLSDTFDTTNREKFRPTYHFSPLYGWMND
PNGMVYKDGEYHLFYQYNPYGSKWGNMNWGHAISKDLVNWEHRPVAIAPDALGTIFSGSAVVDHNNTAGFGAGAIIAIYT
QNSDRQVQSIAYSTDNGRTFTKYENNPVLVSEARDFRDPKVFWYEATKRWIMVLAVGQEMQIFSSPNLKDWAFESSFGEG
YGAHGNVWECPDLFELPVEGTNEKKWVLLCSLGDGPFGDSATQYFIGSFDGKKFSCDNQPNVTKWMDWGKDHYATVTWSD
APDNRRIAIAWMSNWQYANDVPTSQYRSPNSIPRDLSLFAIDGGIYLQSAPSPELLKLRGVSKKRSFKVNGTRIVKDLIP
NNEGAYEIELSLKNQQAEIIGFRLYNDKGEEVDMQYDMKEKKFSMDRRKSGEVNFNENFPMLTWTAIEEDKDEMKLRLFV
DRSSVEAFGDGGRFAMTNQVFPSEPYNHISFYSKGGAYKVDSFVVYKLKK

Specific function: Exo-fructosidase that can hydrolyze both levan and inulin, leading to the production of free fructose. Is also able to hydrolyze sucrose and to a small extent raffinose, but not melezitose, stachylose, cellobiose, maltose, and lactose [H]

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=490, Percent_Identity=37.5510204081633, Blast_Score=270, Evalue=6e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR010496
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR011040 [H]

Pfam domain/function: PF06439 DUF1080; PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.80 [H]

Molecular weight: Translated: 69511; Mature: 69511

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00609 GLYCOSYL_HYDROL_F32

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVFLPVKQLQTFLICFILMTISVSTRAADSPLLIKNLGEGHCLVRVNTSQNYLLLPVED
CCEEECHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEEEECCCCEEEEEECC
ASPDVRISMIVNNKEVKNFDVRLAIHKVDYFVPVDLSDFSGKTVSFKFKMNSNDPIRVNL
CCCCEEEEEEECCCCCCCEEEEEEEEEEEEEEEECCCCCCCCEEEEEEEECCCCCEEEEE
SPDNTACCKEMKLSDTFDTTNREKFRPTYHFSPLYGWMNDPNGMVYKDGEYHLFYQYNPY
CCCCHHHHHHCCCCCCCCCCCCHHCCCEEECCCEECCCCCCCCCEEECCCEEEEEEECCC
GSKWGNMNWGHAISKDLVNWEHRPVAIAPDALGTIFSGSAVVDHNNTAGFGAGAIIAIYT
CCCCCCCCCCHHHHHHHCCCCCCCEEECCCHHHHHCCCCEEEECCCCCCCCCCEEEEEEE
QNSDRQVQSIAYSTDNGRTFTKYENNPVLVSEARDFRDPKVFWYEATKRWIMVLAVGQEM
CCCCCEEEEEEEECCCCEEEEEECCCCEEEECCCCCCCCEEEEEECCCEEEEEEEECCCE
QIFSSPNLKDWAFESSFGEGYGAHGNVWECPDLFELPVEGTNEKKWVLLCSLGDGPFGDS
EEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCEECCCCCCCCCEEEEEEECCCCCCCCC
ATQYFIGSFDGKKFSCDNQPNVTKWMDWGKDHYATVTWSDAPDNRRIAIAWMSNWQYAND
CCEEEEECCCCCEECCCCCCCHHHHHHCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCC
VPTSQYRSPNSIPRDLSLFAIDGGIYLQSAPSPELLKLRGVSKKRSFKVNGTRIVKDLIP
CCCHHCCCCCCCCCCEEEEEEECCEEEECCCCCCEEEEECCCCCCEEEECCEEEEEHHCC
NNEGAYEIELSLKNQQAEIIGFRLYNDKGEEVDMQYDMKEKKFSMDRRKSGEVNFNENFP
CCCCCEEEEEEECCCCEEEEEEEEECCCCCEEEEEECCCHHHHCCCCCCCCCEEECCCCC
MLTWTAIEEDKDEMKLRLFVDRSSVEAFGDGGRFAMTNQVFPSEPYNHISFYSKGGAYKV
EEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCEEE
DSFVVYKLKK
CEEEEEEECC
>Mature Secondary Structure
MNVFLPVKQLQTFLICFILMTISVSTRAADSPLLIKNLGEGHCLVRVNTSQNYLLLPVED
CCEEECHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEEEECCCCEEEEEECC
ASPDVRISMIVNNKEVKNFDVRLAIHKVDYFVPVDLSDFSGKTVSFKFKMNSNDPIRVNL
CCCCEEEEEEECCCCCCCEEEEEEEEEEEEEEEECCCCCCCCEEEEEEEECCCCCEEEEE
SPDNTACCKEMKLSDTFDTTNREKFRPTYHFSPLYGWMNDPNGMVYKDGEYHLFYQYNPY
CCCCHHHHHHCCCCCCCCCCCCHHCCCEEECCCEECCCCCCCCCEEECCCEEEEEEECCC
GSKWGNMNWGHAISKDLVNWEHRPVAIAPDALGTIFSGSAVVDHNNTAGFGAGAIIAIYT
CCCCCCCCCCHHHHHHHCCCCCCCEEECCCHHHHHCCCCEEEECCCCCCCCCCEEEEEEE
QNSDRQVQSIAYSTDNGRTFTKYENNPVLVSEARDFRDPKVFWYEATKRWIMVLAVGQEM
CCCCCEEEEEEEECCCCEEEEEECCCCEEEECCCCCCCCEEEEEECCCEEEEEEEECCCE
QIFSSPNLKDWAFESSFGEGYGAHGNVWECPDLFELPVEGTNEKKWVLLCSLGDGPFGDS
EEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCEECCCCCCCCCEEEEEEECCCCCCCCC
ATQYFIGSFDGKKFSCDNQPNVTKWMDWGKDHYATVTWSDAPDNRRIAIAWMSNWQYAND
CCEEEEECCCCCEECCCCCCCHHHHHHCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCC
VPTSQYRSPNSIPRDLSLFAIDGGIYLQSAPSPELLKLRGVSKKRSFKVNGTRIVKDLIP
CCCHHCCCCCCCCCCEEEEEEECCEEEECCCCCCEEEEECCCCCCEEEECCEEEEEHHCC
NNEGAYEIELSLKNQQAEIIGFRLYNDKGEEVDMQYDMKEKKFSMDRRKSGEVNFNENFP
CCCCCEEEEEEECCCCEEEEEEEEECCCCCEEEEEECCCHHHHCCCCCCCCCEEECCCCC
MLTWTAIEEDKDEMKLRLFVDRSSVEAFGDGGRFAMTNQVFPSEPYNHISFYSKGGAYKV
EEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCCEEE
DSFVVYKLKK
CEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3112519; 3120151; 9141695; 9384377; 2117666 [H]