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The map label for this gene is rfbD [H]

Identifier: 29347140

GI number: 29347140

Start: 2129195

End: 2130043

Strand: Reverse

Name: rfbD [H]

Synonym: BT_1730

Alternate gene names: 29347140

Gene position: 2130043-2129195 (Counterclockwise)

Preceding gene: 29347141

Following gene: 29347139

Centisome position: 34.02

GC content: 44.05

Gene sequence:

>849_bases
ATGAGAATTTTAGTGACGGGTGCCAATGGTCAGCTTGGCAATGAAATGCAAGTTCTTGCAAAAGAGAATCCGCAACATAC
GTATTATTTCACAGATGTGCAGGAGCTGGACATCTGTAATAAGGACGCTGTTTGGGCATATATTGCTGAAAAGCGGATTG
AGCTTATTGTTAATTGTGCGGCATATACAGCAGTAGATAAAGCGGAAGATGATTCGGAACTGGCTTACAAGCTGAACAGT
GAAGCTCCTAAAACGCTGGCATGTGCAGCGCAGTTTAATGGTGCGGCGATGATTCAGGTGTCTACCGACTATGTTTTTGA
CGGCACCGCTCATATTCCTTATACAGAAGAATGCGATCCATGTCCGAATTCGGTATATGGAACAACGAAACTTGAAGGAG
AATATGAGGTACTGAATCATTGCGAAAAGTCAGTCGTTATCCGTACCGCATGGCTTTATTCCACTTTTGGCAACAACTTC
GTGAAGACGATGATCCGTCTGGGCAAAGAGCGTGATAGTCTGGGAGTCATTTTCGATCAGGTAGGAACTCCTACTTACGC
CAATGACCTGGCACAGGCCATCTTTGCTATTATTAATAAAGGCATCGTTCGTGGAGTCTATCATTTCAGCAACGAAGGAG
TCTGCTCCTGGTATGACTTTACGGTAGCTATTCATCGTTTGGCAGGAATCACTTCATGCAAAGTGAAACCGCTGCATACG
GCGGAATATCCTACAAGAGCGAACCGCCCTGCATATTCCGTACTCGATAAAACAAAAATAAAAACAACGTTCGGCATCGA
AATACCTCATTGGGAGGAAAGCCTGAAAAGATGCATCGATACTTTATAA

Upstream 100 bases:

>100_bases
GGGGACTATGAAGGCGGTAGAAGCTATCAGCAGCTTTCTTTCCATGTTGGCAAACTATTACGATAAAGACCTGAAAGGGG
AATTAAAACTTGATGAATAA

Downstream 100 bases:

>100_bases
TAAATATGGCAGATAATACAGAAATACAGAGACGGCGAACATTCGCCATTATTGCGCATCCGGATGCCGGTAAGACATCA
TTGACGGAGAAGTTATTGCT

Product: putative dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MRILVTGANGQLGNEMQVLAKENPQHTYYFTDVQELDICNKDAVWAYIAEKRIELIVNCAAYTAVDKAEDDSELAYKLNS
EAPKTLACAAQFNGAAMIQVSTDYVFDGTAHIPYTEECDPCPNSVYGTTKLEGEYEVLNHCEKSVVIRTAWLYSTFGNNF
VKTMIRLGKERDSLGVIFDQVGTPTYANDLAQAIFAIINKGIVRGVYHFSNEGVCSWYDFTVAIHRLAGITSCKVKPLHT
AEYPTRANRPAYSVLDKTKIKTTFGIEIPHWEESLKRCIDTL

Sequences:

>Translated_282_residues
MRILVTGANGQLGNEMQVLAKENPQHTYYFTDVQELDICNKDAVWAYIAEKRIELIVNCAAYTAVDKAEDDSELAYKLNS
EAPKTLACAAQFNGAAMIQVSTDYVFDGTAHIPYTEECDPCPNSVYGTTKLEGEYEVLNHCEKSVVIRTAWLYSTFGNNF
VKTMIRLGKERDSLGVIFDQVGTPTYANDLAQAIFAIINKGIVRGVYHFSNEGVCSWYDFTVAIHRLAGITSCKVKPLHT
AEYPTRANRPAYSVLDKTKIKTTFGIEIPHWEESLKRCIDTL
>Mature_282_residues
MRILVTGANGQLGNEMQVLAKENPQHTYYFTDVQELDICNKDAVWAYIAEKRIELIVNCAAYTAVDKAEDDSELAYKLNS
EAPKTLACAAQFNGAAMIQVSTDYVFDGTAHIPYTEECDPCPNSVYGTTKLEGEYEVLNHCEKSVVIRTAWLYSTFGNNF
VKTMIRLGKERDSLGVIFDQVGTPTYANDLAQAIFAIINKGIVRGVYHFSNEGVCSWYDFTVAIHRLAGITSCKVKPLHT
AEYPTRANRPAYSVLDKTKIKTTFGIEIPHWEESLKRCIDTL

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=285, Percent_Identity=29.8245614035088, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI33519455, Length=285, Percent_Identity=29.8245614035088, Blast_Score=105, Evalue=5e-23,
Organism=Escherichia coli, GI1788352, Length=296, Percent_Identity=40.5405405405405, Blast_Score=198, Evalue=3e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 31550; Mature: 31550

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILVTGANGQLGNEMQVLAKENPQHTYYFTDVQELDICNKDAVWAYIAEKRIELIVNCA
CEEEEECCCCCCCCCEEEEECCCCCCEEEECCHHHHCCCCCCCCHHHHHHHHHHHHHHHH
AYTAVDKAEDDSELAYKLNSEAPKTLACAAQFNGAAMIQVSTDYVFDGTAHIPYTEECDP
HHHHHCCCCCCCHHEEEECCCCCCEEEEEEECCCEEEEEEECCEEECCEECCCCCCCCCC
CPNSVYGTTKLEGEYEVLNHCEKSVVIRTAWLYSTFGNNFVKTMIRLGKERDSLGVIFDQ
CCCCCCCCEEECCCHHHHHHCCHHEEEEHHHHHHHHCHHHHHHHHHHCCCCCCCEEEECC
VGTPTYANDLAQAIFAIINKGIVRGVYHFSNEGVCSWYDFTVAIHRLAGITSCKVKPLHT
CCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEHHHHHHHHHHHHCCCCCCCCCCCC
AEYPTRANRPAYSVLDKTKIKTTFGIEIPHWEESLKRCIDTL
CCCCCCCCCCHHHHHHHHEEEEEEEEECCCHHHHHHHHHHCC
>Mature Secondary Structure
MRILVTGANGQLGNEMQVLAKENPQHTYYFTDVQELDICNKDAVWAYIAEKRIELIVNCA
CEEEEECCCCCCCCCEEEEECCCCCCEEEECCHHHHCCCCCCCCHHHHHHHHHHHHHHHH
AYTAVDKAEDDSELAYKLNSEAPKTLACAAQFNGAAMIQVSTDYVFDGTAHIPYTEECDP
HHHHHCCCCCCCHHEEEECCCCCCEEEEEEECCCEEEEEEECCEEECCEECCCCCCCCCC
CPNSVYGTTKLEGEYEVLNHCEKSVVIRTAWLYSTFGNNFVKTMIRLGKERDSLGVIFDQ
CCCCCCCCEEECCCHHHHHHCCHHEEEEHHHHHHHHCHHHHHHHHHHCCCCCCCEEEECC
VGTPTYANDLAQAIFAIINKGIVRGVYHFSNEGVCSWYDFTVAIHRLAGITSCKVKPLHT
CCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEHHHHHHHHHHHHCCCCCCCCCCCC
AEYPTRANRPAYSVLDKTKIKTTFGIEIPHWEESLKRCIDTL
CCCCCCCCCCHHHHHHHHEEEEEEEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]