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The map label for this gene is prfC [H]

Identifier: 29347139

GI number: 29347139

Start: 2127615

End: 2129213

Strand: Reverse

Name: prfC [H]

Synonym: BT_1729

Alternate gene names: 29347139

Gene position: 2129213-2127615 (Counterclockwise)

Preceding gene: 29347140

Following gene: 29347138

Centisome position: 34.01

GC content: 47.15

Gene sequence:

>1599_bases
ATGCATCGATACTTTATAATAAATATGGCAGATAATACAGAAATACAGAGACGGCGAACATTCGCCATTATTGCGCATCC
GGATGCCGGTAAGACATCATTGACGGAGAAGTTATTGCTTTTCGGTGGTCAGATTCAGGTGGCAGGTGCGGTCAAGAGCA
ATAAAATCAAGAAAACGGCTACTTCCGACTGGATGGAGATTGAGAAACAGCGTGGTATCTCGGTGACAACTTCCGTAATG
GAATTTGACTATCGGGACTACAAGATCAATATTCTTGATACTCCGGGTCACCAGGACTTTGCCGAAGACACATACCGTAC
GCTGACTGCTGTAGACAGCGTTATCATTGTAGTAGACGGAGCGAAAGGTGTGGAAACACAAACCCGTAAACTGATGGAAG
TGTGCCGTATGAGAAAAACTCCGGTCATCATTTTCGTTAATAAAATGGACCGTGAAGGTAAGGACCCGTTTGACTTGCTG
GACGAGCTGGAAGAAGAACTGATGATTCAGGTCCGCCCGCTGTCATGGCCTATCGAACAGGGAGCCCGCTTCAAAGGAGT
ATATAATATTTATGAGAAGAAACTGGACTTGTACCAACCTTCCAAACAAGTGGTGACGGAGAAGGTAGAAGTCGACATAC
ATACGGAAGAACTGGATAAGCAGATCGGTAAGCCGCTGGCAGACAAACTGCGTGGCGATCTGGAACTGATCGAAGGAGTT
TATCCGGAACTGGATGTGGAATCTTATCTGGCAGGAGATTGCGCGCCTGTATTCTTTGGCTCTGCCTTGAATAATTTTGG
TGTACAGGAATTGTTGAACTGCTTTGTGGAGATTGCTCCCAGTCCTCGTCCTGTACAGGCAGAAGAGCGTGAAGTGAAAC
CGGACGAACCGAAATTCACCGGATTCATCTTTAAGATTACCGCCAATATCGACCCGAATCATCGTTCGTGTGTAGCATTC
TGCAAGATATGTTCGGGCAAATTTGTACGTAATGCACCCTATCAGCACGTCCGTCATGGCAAGACCATGCGTTTCTCATC
GCCCACCCAGTTTATGGCGCAGCGGAAAACGACCATTGACGAAGCCTATGCAGGAGATATCATCGGTTTGCCGGACAACG
GAACTTTCAAGATCGGTGATACGCTGACGGAAGGAGAACTGCTCCATTTCCGCGGCTTGCCCAGCTTCTCGCCGGAGATG
TTCAAGTATATCGAAAATGCCGATCCGATGAAGCAGAAACAGCTGGCAAAGGGTATCGACCAGTTGATGGACGAAGGTGT
GGCACAGTTGTTTGTCAATCAGTTCAACGGCCGTAAGATTATCGGTACGGTCGGACAGTTGCAGTTTGAGGTTATCCAGT
ATCGTCTGCTGAACGAGTACAATGCTTCTTGCCGTTGGGAACCGGTAAGTTTGTACAAGGCTTGTTGGGTAGAAAGCGAT
GATCCTGCTGAGCTGGAAGCTTTCAAGAAACGTAAATACCAATATATGGCGAAGGACAGGGAAGGACGTGACGTATTCCT
TGCCGATTCAGGCTATGTGCTTCAGATGGCACAAATGGACTTCAAGCACATTAAATTCCACTTCACAAGTGAATTTTAG

Upstream 100 bases:

>100_bases
CTACAAGAGCGAACCGCCCTGCATATTCCGTACTCGATAAAACAAAAATAAAAACAACGTTCGGCATCGAAATACCTCAT
TGGGAGGAAAGCCTGAAAAG

Downstream 100 bases:

>100_bases
TCTGCTTGGTGGAATTTAACAAAAATAATTGGTAAAAGGGGAAGAATTATGTTACCTGTAAATAAGGGAAGGAGAAATGA
ATACAACATTTTGGGAGAAA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MHRYFIINMADNTEIQRRRTFAIIAHPDAGKTSLTEKLLLFGGQIQVAGAVKSNKIKKTATSDWMEIEKQRGISVTTSVM
EFDYRDYKINILDTPGHQDFAEDTYRTLTAVDSVIIVVDGAKGVETQTRKLMEVCRMRKTPVIIFVNKMDREGKDPFDLL
DELEEELMIQVRPLSWPIEQGARFKGVYNIYEKKLDLYQPSKQVVTEKVEVDIHTEELDKQIGKPLADKLRGDLELIEGV
YPELDVESYLAGDCAPVFFGSALNNFGVQELLNCFVEIAPSPRPVQAEEREVKPDEPKFTGFIFKITANIDPNHRSCVAF
CKICSGKFVRNAPYQHVRHGKTMRFSSPTQFMAQRKTTIDEAYAGDIIGLPDNGTFKIGDTLTEGELLHFRGLPSFSPEM
FKYIENADPMKQKQLAKGIDQLMDEGVAQLFVNQFNGRKIIGTVGQLQFEVIQYRLLNEYNASCRWEPVSLYKACWVESD
DPAELEAFKKRKYQYMAKDREGRDVFLADSGYVLQMAQMDFKHIKFHFTSEF

Sequences:

>Translated_532_residues
MHRYFIINMADNTEIQRRRTFAIIAHPDAGKTSLTEKLLLFGGQIQVAGAVKSNKIKKTATSDWMEIEKQRGISVTTSVM
EFDYRDYKINILDTPGHQDFAEDTYRTLTAVDSVIIVVDGAKGVETQTRKLMEVCRMRKTPVIIFVNKMDREGKDPFDLL
DELEEELMIQVRPLSWPIEQGARFKGVYNIYEKKLDLYQPSKQVVTEKVEVDIHTEELDKQIGKPLADKLRGDLELIEGV
YPELDVESYLAGDCAPVFFGSALNNFGVQELLNCFVEIAPSPRPVQAEEREVKPDEPKFTGFIFKITANIDPNHRSCVAF
CKICSGKFVRNAPYQHVRHGKTMRFSSPTQFMAQRKTTIDEAYAGDIIGLPDNGTFKIGDTLTEGELLHFRGLPSFSPEM
FKYIENADPMKQKQLAKGIDQLMDEGVAQLFVNQFNGRKIIGTVGQLQFEVIQYRLLNEYNASCRWEPVSLYKACWVESD
DPAELEAFKKRKYQYMAKDREGRDVFLADSGYVLQMAQMDFKHIKFHFTSEF
>Mature_532_residues
MHRYFIINMADNTEIQRRRTFAIIAHPDAGKTSLTEKLLLFGGQIQVAGAVKSNKIKKTATSDWMEIEKQRGISVTTSVM
EFDYRDYKINILDTPGHQDFAEDTYRTLTAVDSVIIVVDGAKGVETQTRKLMEVCRMRKTPVIIFVNKMDREGKDPFDLL
DELEEELMIQVRPLSWPIEQGARFKGVYNIYEKKLDLYQPSKQVVTEKVEVDIHTEELDKQIGKPLADKLRGDLELIEGV
YPELDVESYLAGDCAPVFFGSALNNFGVQELLNCFVEIAPSPRPVQAEEREVKPDEPKFTGFIFKITANIDPNHRSCVAF
CKICSGKFVRNAPYQHVRHGKTMRFSSPTQFMAQRKTTIDEAYAGDIIGLPDNGTFKIGDTLTEGELLHFRGLPSFSPEM
FKYIENADPMKQKQLAKGIDQLMDEGVAQLFVNQFNGRKIIGTVGQLQFEVIQYRLLNEYNASCRWEPVSLYKACWVESD
DPAELEAFKKRKYQYMAKDREGRDVFLADSGYVLQMAQMDFKHIKFHFTSEF

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=485, Percent_Identity=26.5979381443299, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI19923640, Length=511, Percent_Identity=25.440313111546, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI25306283, Length=396, Percent_Identity=27.2727272727273, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI25306287, Length=483, Percent_Identity=25.2587991718427, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI157426893, Length=155, Percent_Identity=32.258064516129, Blast_Score=90, Evalue=7e-18,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=32.1167883211679, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=27.5167785234899, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI310132016, Length=116, Percent_Identity=35.3448275862069, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI310110807, Length=116, Percent_Identity=35.3448275862069, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI310123363, Length=116, Percent_Identity=35.3448275862069, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI217272892, Length=152, Percent_Identity=26.9736842105263, Blast_Score=67, Evalue=6e-11,
Organism=Homo sapiens, GI217272894, Length=152, Percent_Identity=26.9736842105263, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI1790835, Length=520, Percent_Identity=49.8076923076923, Blast_Score=531, Evalue=1e-152,
Organism=Escherichia coli, GI1789738, Length=494, Percent_Identity=29.1497975708502, Blast_Score=166, Evalue=5e-42,
Organism=Escherichia coli, GI48994988, Length=206, Percent_Identity=33.495145631068, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1788922, Length=152, Percent_Identity=32.8947368421053, Blast_Score=93, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=500, Percent_Identity=27.2, Blast_Score=165, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17556745, Length=178, Percent_Identity=34.8314606741573, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17557151, Length=150, Percent_Identity=30.6666666666667, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=27.3291925465839, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=30.5970149253731, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=30.5970149253731, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323098, Length=479, Percent_Identity=25.0521920668058, Blast_Score=146, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6322359, Length=532, Percent_Identity=23.6842105263158, Blast_Score=131, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6324707, Length=149, Percent_Identity=27.5167785234899, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320593, Length=149, Percent_Identity=27.5167785234899, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323320, Length=169, Percent_Identity=28.4023668639053, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24582462, Length=496, Percent_Identity=26.4112903225806, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI221458488, Length=511, Percent_Identity=25.6360078277886, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI78706572, Length=154, Percent_Identity=32.4675324675325, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24585711, Length=147, Percent_Identity=31.9727891156463, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24585713, Length=147, Percent_Identity=31.9727891156463, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24585709, Length=147, Percent_Identity=31.9727891156463, Blast_Score=77, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 60904; Mature: 60904

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRYFIINMADNTEIQRRRTFAIIAHPDAGKTSLTEKLLLFGGQIQVAGAVKSNKIKKTA
CCEEEEEEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCHHH
TSDWMEIEKQRGISVTTSVMEFDYRDYKINILDTPGHQDFAEDTYRTLTAVDSVIIVVDG
HHHHHHHHHHCCCEEEEHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCEEEEEEEC
AKGVETQTRKLMEVCRMRKTPVIIFVNKMDREGKDPFDLLDELEEELMIQVRPLSWPIEQ
CCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHEEEEECCCCCHHC
GARFKGVYNIYEKKLDLYQPSKQVVTEKVEVDIHTEELDKQIGKPLADKLRGDLELIEGV
CCCCHHHHHHHHHHHHHCCCHHHHHHHHEEEEEEHHHHHHHHCCHHHHHHCCHHHHHHCC
YPELDVESYLAGDCAPVFFGSALNNFGVQELLNCFVEIAPSPRPVQAEEREVKPDEPKFT
CCCCCHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCEE
GFIFKITANIDPNHRSCVAFCKICSGKFVRNAPYQHVRHGKTMRFSSPTQFMAQRKTTID
EEEEEEEECCCCCHHHHHHHHHHHCCCHHCCCCHHHHHCCCEEEECCCHHHHHHHHHHHH
EAYAGDIIGLPDNGTFKIGDTLTEGELLHFRGLPSFSPEMFKYIENADPMKQKQLAKGID
HHHCCCEEECCCCCCEEECCCCCCCCEEEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHH
QLMDEGVAQLFVNQFNGRKIIGTVGQLQFEVIQYRLLNEYNASCRWEPVSLYKACWVESD
HHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCC
DPAELEAFKKRKYQYMAKDREGRDVFLADSGYVLQMAQMDFKHIKFHFTSEF
CCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCCEEEEEEEECCC
>Mature Secondary Structure
MHRYFIINMADNTEIQRRRTFAIIAHPDAGKTSLTEKLLLFGGQIQVAGAVKSNKIKKTA
CCEEEEEEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCHHH
TSDWMEIEKQRGISVTTSVMEFDYRDYKINILDTPGHQDFAEDTYRTLTAVDSVIIVVDG
HHHHHHHHHHCCCEEEEHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCEEEEEEEC
AKGVETQTRKLMEVCRMRKTPVIIFVNKMDREGKDPFDLLDELEEELMIQVRPLSWPIEQ
CCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHEEEEECCCCCHHC
GARFKGVYNIYEKKLDLYQPSKQVVTEKVEVDIHTEELDKQIGKPLADKLRGDLELIEGV
CCCCHHHHHHHHHHHHHCCCHHHHHHHHEEEEEEHHHHHHHHCCHHHHHHCCHHHHHHCC
YPELDVESYLAGDCAPVFFGSALNNFGVQELLNCFVEIAPSPRPVQAEEREVKPDEPKFT
CCCCCHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCEE
GFIFKITANIDPNHRSCVAFCKICSGKFVRNAPYQHVRHGKTMRFSSPTQFMAQRKTTID
EEEEEEEECCCCCHHHHHHHHHHHCCCHHCCCCHHHHHCCCEEEECCCHHHHHHHHHHHH
EAYAGDIIGLPDNGTFKIGDTLTEGELLHFRGLPSFSPEMFKYIENADPMKQKQLAKGID
HHHCCCEEECCCCCCEEECCCCCCCCEEEECCCCCCCHHHHHHHCCCCCHHHHHHHHHHH
QLMDEGVAQLFVNQFNGRKIIGTVGQLQFEVIQYRLLNEYNASCRWEPVSLYKACWVESD
HHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCC
DPAELEAFKKRKYQYMAKDREGRDVFLADSGYVLQMAQMDFKHIKFHFTSEF
CCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA