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The map label for this gene is pycB [H]

Identifier: 29347107

GI number: 29347107

Start: 2095697

End: 2097526

Strand: Reverse

Name: pycB [H]

Synonym: BT_1697

Alternate gene names: 29347107

Gene position: 2097526-2095697 (Counterclockwise)

Preceding gene: 29347108

Following gene: 29347106

Centisome position: 33.5

GC content: 49.18

Gene sequence:

>1830_bases
ATGAAAAGAGAAGTTAAGTTTAGTCTGGTTTTCCGAGATATGTGGCAATCTGCCGGAAAATATGTACCTCGTGTAGACCA
ACTCGTAAAGGTAGCTCCTGCCATCATTGAAATGGGCTGTTTCGCCCGTGTAGAAACAAATGGCGGAGGTTTTGAACAAG
TGAATTTATTGTTTGGTGAAAACCCGAATAGAGCCGTGCGCGAATGGACGAAGCCGTTCCACGAAGCCGGCATACAGACA
CACATGCTCGACCGTGCATTGAACGGACTCCGTATGAGCCCCGTTCCGGCAGACGTCCGCAAGTTGTTCTACAAAGTTAA
GAAAGCACAAGGCACAGATATCACCCGTACATTCTGCGGATTGAACGACGTACGCAATATCGCTCCTTCCATCACGTATG
CCAAAGAGGCAGGCATGATCTCACAGTGTTCACTCTGCATCACCCATTCGCCGATCCACACGGTAGAGTACTACACCAAT
ATGGCGTTAGAACTTATCAAGCTGGGGGCGGACGAGATTTGCATCAAAGACATGGCAGGTATCGGTCGCCCCGTTTCATT
GGGCAAGATCGTGGCCAATATCAAAGCGGCACATCCTGAAATTCCTATTCAGTATCATAGTCATGCCGGTCCCGGTTTCA
ACATGGCAAGCATCCTCGAAGTATGCGAAGCCGGTTGCGACTACATTGACGTAGGTATGGAACCGCTTTCATGGGGTACG
GGACACGCCGATCTTCTCAGTGTACAGGCTATGCTGAAAGATGCCGGATATCAGGTACCGGAAATCAATATGGAAGCCTA
CATGAAAGTGCGTGCGCTGATTCAAGAATTTATGGATGACTTCCTCGGCTTGTATATCAGTCCGAAGAACCGTCTGATGA
ACTCGCTGCTGATCGGTCCGGGACTTCCTGGCGGTATGATGGGTAGTCTGATGGCTGACCTCGAATCGAATCTGGAATCT
ATCAACAAGTATAAAGCCAAACATAACCTGCCGTTTATGACACAGGATCAGCTGCTGATCAAGCTATTCAACGAAGTGGC
GTATGTATGGCCCCGCGTCGGTTATCCTCCATTGGTAACTCCGTTCAGCCAGTACGTGAAGAACCTTGCCATGATGAATG
TGATGGCAATGGAAAAAGGCAAGGAACGCTGGGGAATGATTGCCGACGATATCTGGGATATGCTGCTGGGTAAAGCAGGA
CGCCTGCCGGGTACACTGGCTCCCGAAATCATCGAGAAAGCCGAACGTGAAGGACGTAAATTCTTCGAAGGTAATCCGCA
GGACAATTACCCTGACTCACTCGACAAGTATCGCAAGCTGATGAAGGAAAACAAATGGGAAGTGGGAGAGGACGACGAAG
AACTCTTCGAATATGCCATGCATCCGGCTCAGTATGAGGCTTACAAGAGTGGAAAAGCGAAGGAAGACTTCCTGGCAGAC
GTGGCAAAACGTCGTGCCGAGAAAGATAAATCGCCGGAAGAAGATGCAAAACCAAAAACACTGACTGTACAGGTGGACGG
ACAGGCTTATCGTGTGACGGTAGCATACGGAGATGCCGAACTGCCTGCTGCACCTGTAGCTGCAGCTCCGGCAGGAGAGG
GCAAAGATGTTCTTTCTCCGCTGGAAGGTAAATTCTTCCTGGTGAAGAATGCGCAGGAATCTGCCTTGAAAGTTGGCGAT
GCGGTGAAAGAGGGCGATGTGCTTTGTTATGTGGAAGCAATGAAGACTTACAACGCTATCCGTGCCGAATTTAGCGGTAC
GATAACTGCTATCTGCGCTAATCCGGGAGACACTGTTTCAGAAGATGATGTATTAATGAAGATAGGATAA

Upstream 100 bases:

>100_bases
GCGGCTATCACAGCAGCTGTGAATGTGGTGACATTAGGAAAAGGAAAGATTACAAAAGTAGAAAAGTTATAAACCTATCA
AAAGTATATCATAGAAAAAC

Downstream 100 bases:

>100_bases
TGAACGAGATATTTGAGAACTTATATGACATGACTGCGTTCAGCAATATCATTGCCGAACCGCAGTTTCTGATAATGTAT
GCCATCGCGTTCGTCCTGCT

Product: pyruvate carboxylase subunit B

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 609; Mature: 609

Protein sequence:

>609_residues
MKREVKFSLVFRDMWQSAGKYVPRVDQLVKVAPAIIEMGCFARVETNGGGFEQVNLLFGENPNRAVREWTKPFHEAGIQT
HMLDRALNGLRMSPVPADVRKLFYKVKKAQGTDITRTFCGLNDVRNIAPSITYAKEAGMISQCSLCITHSPIHTVEYYTN
MALELIKLGADEICIKDMAGIGRPVSLGKIVANIKAAHPEIPIQYHSHAGPGFNMASILEVCEAGCDYIDVGMEPLSWGT
GHADLLSVQAMLKDAGYQVPEINMEAYMKVRALIQEFMDDFLGLYISPKNRLMNSLLIGPGLPGGMMGSLMADLESNLES
INKYKAKHNLPFMTQDQLLIKLFNEVAYVWPRVGYPPLVTPFSQYVKNLAMMNVMAMEKGKERWGMIADDIWDMLLGKAG
RLPGTLAPEIIEKAEREGRKFFEGNPQDNYPDSLDKYRKLMKENKWEVGEDDEELFEYAMHPAQYEAYKSGKAKEDFLAD
VAKRRAEKDKSPEEDAKPKTLTVQVDGQAYRVTVAYGDAELPAAPVAAAPAGEGKDVLSPLEGKFFLVKNAQESALKVGD
AVKEGDVLCYVEAMKTYNAIRAEFSGTITAICANPGDTVSEDDVLMKIG

Sequences:

>Translated_609_residues
MKREVKFSLVFRDMWQSAGKYVPRVDQLVKVAPAIIEMGCFARVETNGGGFEQVNLLFGENPNRAVREWTKPFHEAGIQT
HMLDRALNGLRMSPVPADVRKLFYKVKKAQGTDITRTFCGLNDVRNIAPSITYAKEAGMISQCSLCITHSPIHTVEYYTN
MALELIKLGADEICIKDMAGIGRPVSLGKIVANIKAAHPEIPIQYHSHAGPGFNMASILEVCEAGCDYIDVGMEPLSWGT
GHADLLSVQAMLKDAGYQVPEINMEAYMKVRALIQEFMDDFLGLYISPKNRLMNSLLIGPGLPGGMMGSLMADLESNLES
INKYKAKHNLPFMTQDQLLIKLFNEVAYVWPRVGYPPLVTPFSQYVKNLAMMNVMAMEKGKERWGMIADDIWDMLLGKAG
RLPGTLAPEIIEKAEREGRKFFEGNPQDNYPDSLDKYRKLMKENKWEVGEDDEELFEYAMHPAQYEAYKSGKAKEDFLAD
VAKRRAEKDKSPEEDAKPKTLTVQVDGQAYRVTVAYGDAELPAAPVAAAPAGEGKDVLSPLEGKFFLVKNAQESALKVGD
AVKEGDVLCYVEAMKTYNAIRAEFSGTITAICANPGDTVSEDDVLMKIG
>Mature_609_residues
MKREVKFSLVFRDMWQSAGKYVPRVDQLVKVAPAIIEMGCFARVETNGGGFEQVNLLFGENPNRAVREWTKPFHEAGIQT
HMLDRALNGLRMSPVPADVRKLFYKVKKAQGTDITRTFCGLNDVRNIAPSITYAKEAGMISQCSLCITHSPIHTVEYYTN
MALELIKLGADEICIKDMAGIGRPVSLGKIVANIKAAHPEIPIQYHSHAGPGFNMASILEVCEAGCDYIDVGMEPLSWGT
GHADLLSVQAMLKDAGYQVPEINMEAYMKVRALIQEFMDDFLGLYISPKNRLMNSLLIGPGLPGGMMGSLMADLESNLES
INKYKAKHNLPFMTQDQLLIKLFNEVAYVWPRVGYPPLVTPFSQYVKNLAMMNVMAMEKGKERWGMIADDIWDMLLGKAG
RLPGTLAPEIIEKAEREGRKFFEGNPQDNYPDSLDKYRKLMKENKWEVGEDDEELFEYAMHPAQYEAYKSGKAKEDFLAD
VAKRRAEKDKSPEEDAKPKTLTVQVDGQAYRVTVAYGDAELPAAPVAAAPAGEGKDVLSPLEGKFFLVKNAQESALKVGD
AVKEGDVLCYVEAMKTYNAIRAEFSGTITAICANPGDTVSEDDVLMKIG

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=654, Percent_Identity=24.1590214067278, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI106049295, Length=654, Percent_Identity=24.1590214067278, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI106049292, Length=654, Percent_Identity=24.1590214067278, Blast_Score=107, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17562816, Length=644, Percent_Identity=22.6708074534161, Blast_Score=103, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6319695, Length=644, Percent_Identity=22.5155279503106, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321376, Length=639, Percent_Identity=22.6917057902973, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI281363050, Length=645, Percent_Identity=24.3410852713178, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652224, Length=645, Percent_Identity=24.3410852713178, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652222, Length=645, Percent_Identity=24.3410852713178, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652220, Length=645, Percent_Identity=24.3410852713178, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652218, Length=645, Percent_Identity=24.3410852713178, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652212, Length=645, Percent_Identity=24.3410852713178, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652210, Length=645, Percent_Identity=24.3410852713178, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652214, Length=645, Percent_Identity=24.3410852713178, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19921944, Length=645, Percent_Identity=24.3410852713178, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652216, Length=645, Percent_Identity=24.3410852713178, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 67560; Mature: 67560

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS50991 PYR_CT ; PS50968 BIOTINYL_LIPOYL ; PS00188 BIOTIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKREVKFSLVFRDMWQSAGKYVPRVDQLVKVAPAIIEMGCFARVETNGGGFEQVNLLFGE
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEECC
NPNRAVREWTKPFHEAGIQTHMLDRALNGLRMSPVPADVRKLFYKVKKAQGTDITRTFCG
CCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHC
LNDVRNIAPSITYAKEAGMISQCSLCITHSPIHTVEYYTNMALELIKLGADEICIKDMAG
HHHHHHHCCCCHHHHHCCCHHHHHEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHC
IGRPVSLGKIVANIKAAHPEIPIQYHSHAGPGFNMASILEVCEAGCDYIDVGMEPLSWGT
CCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCC
GHADLLSVQAMLKDAGYQVPEINMEAYMKVRALIQEFMDDFLGLYISPKNRLMNSLLIGP
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCEEECCHHHHHHHHHCCC
GLPGGMMGSLMADLESNLESINKYKAKHNLPFMTQDQLLIKLFNEVAYVWPRVGYPPLVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
PFSQYVKNLAMMNVMAMEKGKERWGMIADDIWDMLLGKAGRLPGTLAPEIIEKAEREGRK
HHHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCH
FFEGNPQDNYPDSLDKYRKLMKENKWEVGEDDEELFEYAMHPAQYEAYKSGKAKEDFLAD
HCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHCCCCHHHHHHH
VAKRRAEKDKSPEEDAKPKTLTVQVDGQAYRVTVAYGDAELPAAPVAAAPAGEGKDVLSP
HHHHHHHCCCCCCCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHCC
LEGKFFLVKNAQESALKVGDAVKEGDVLCYVEAMKTYNAIRAEFSGTITAICANPGDTVS
CCCCEEEEECCHHHHHHHHCHHCCCCEEEEHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
EDDVLMKIG
CCCEEEECC
>Mature Secondary Structure
MKREVKFSLVFRDMWQSAGKYVPRVDQLVKVAPAIIEMGCFARVETNGGGFEQVNLLFGE
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEECC
NPNRAVREWTKPFHEAGIQTHMLDRALNGLRMSPVPADVRKLFYKVKKAQGTDITRTFCG
CCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHC
LNDVRNIAPSITYAKEAGMISQCSLCITHSPIHTVEYYTNMALELIKLGADEICIKDMAG
HHHHHHHCCCCHHHHHCCCHHHHHEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHC
IGRPVSLGKIVANIKAAHPEIPIQYHSHAGPGFNMASILEVCEAGCDYIDVGMEPLSWGT
CCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCC
GHADLLSVQAMLKDAGYQVPEINMEAYMKVRALIQEFMDDFLGLYISPKNRLMNSLLIGP
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCEEECCHHHHHHHHHCCC
GLPGGMMGSLMADLESNLESINKYKAKHNLPFMTQDQLLIKLFNEVAYVWPRVGYPPLVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
PFSQYVKNLAMMNVMAMEKGKERWGMIADDIWDMLLGKAGRLPGTLAPEIIEKAEREGRK
HHHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCH
FFEGNPQDNYPDSLDKYRKLMKENKWEVGEDDEELFEYAMHPAQYEAYKSGKAKEDFLAD
HCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHCCCCHHHHHHH
VAKRRAEKDKSPEEDAKPKTLTVQVDGQAYRVTVAYGDAELPAAPVAAAPAGEGKDVLSP
HHHHHHHCCCCCCCCCCCCEEEEEECCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHCC
LEGKFFLVKNAQESALKVGDAVKEGDVLCYVEAMKTYNAIRAEFSGTITAICANPGDTVS
CCCCEEEEECCHHHHHHHHCHHCCCCEEEEHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
EDDVLMKIG
CCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]