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The map label for this gene is pulA [H]

Identifier: 29347073

GI number: 29347073

Start: 2055649

End: 2057655

Strand: Reverse

Name: pulA [H]

Synonym: BT_1663

Alternate gene names: 29347073

Gene position: 2057655-2055649 (Counterclockwise)

Preceding gene: 161511192

Following gene: 29347072

Centisome position: 32.87

GC content: 47.23

Gene sequence:

>2007_bases
ATGAAAATGGGAACCAATTATTTAGCGATATTAGGGGTGACTACGGTGACGACAGTAATGAGTTGTACTACTGCGAAAAA
GGAATATATGTCTTATGAATTATATCCGGTCCGTACAGGAAGTCTGATTGAAATGGAGTATACACCGGAGGCAACGAAGT
TTACTCTTTGGTCGCCTACGGCAGATGAAGTACGTCTGATGCTTTATGAGGCAGGCGAGGGCGGACATGCCTATGAAACG
GTAAAGATGCAGTCGGGGGAAGAAGGAACCTGGACAGCTGTGGTAAGTAAGGACCTTATTGGCAAGTTCTATACTTTCAA
TGTGAAGATCGATGACAAGTGGCAGGGAGATACTCCCGGAATAAACGCCCGTGCTGTCGGAGTAAACGGGAAGCGGGCCG
CTATTATCGACTGGCAATCCACCAATCCGGACGGTTGGGAGAGTGACACCCGACCTCCTTTGAAGTCTCCCGCCGATATG
ATTATTTATGAGATGCACCACCGTGACTTTTCGGTCGATTCTACTTCAGGAGTGAAGAATAAAGGAAAATATCTGGCACT
GACCGAACATGGAACGATGAACTCCGACAAGTTACTGACAGGTATCGACCATCTGATCGAACTGGGCGTGACACACGTGC
ATCTGCTTCCTTCTTTTGATTACGCTTCGGTAGATGAAACCAGACTGAATGAAAATTCGTACAACTGGGGATATGATCCG
CAAAACTATAATGTACCCGATGGTTCCTATGCCACTGATCCCTATCAGCCCGCCACTCGTGTGAAAGAATTCAAGCAGAT
GGTACAGGCACTGCACAAAGCTGGTATCCGTGTGATAATGGATGTGGTGTACAATCATACATTCAATACCGATGAGAGTA
ACTTCGAGCGCACCGTTCCCGGTTATTTCTATCGCCAGAAAGAAGATAAAACCTTGGCAAATGGCTCAGGCTGTGGTAAC
GAGACTGCCAGCGAACGCCTTATGATGCGTAAGTTTATGGTAGAATCCGTCCTTTATTGGATAAAAGAATATCATGTTGA
CGGCTTCCGGTTCGACTTGATGGGAATTCATGACATAGAAACGATGAACGAGATACGGAAGGCTGTAAATGCGGTCGATC
CTACCATTTGCATTTACGGAGAGGGGTGGGCTGCCGAGGCGCCTCAGTATCCGGCTGACTCCTTGGCAATGAAGGGAAAT
ATAGCACAGATACCCGGAGTTGCTGTCTTTTCTGACGAGCTGCGTGACGGGCTTTGCGGACCTGTAGGCGATAAGCGCAA
AGGAGCTTTTCTTGCCGGAATTCCGGGAGGGGAGATGAGCGTAAAGTTCGGCATAGCAGGCGCTATCGAGCATCCGCAAG
TACAGTGCGATTCGGTTAATTATACTCAAAAGCCGTGGGCGAAACAGCCTGTGCAAATGATCAGTTATGTTTCCTGTCAC
GACGGGCTGTGTTTGGTAGACCGACTGAAGGCAAGCATGCCGGACATCACTCCCGAACAACTTATACGTCTCGATAAACT
GGCACAGACTGTGGTCTTTACTTCACAAGGTATTCCGTTTATTTACGCCGGAGAAGAAATCATGCGTGATAAGCAAGGCG
TGGACAATAGTTATAAGAGCCCGGATGCAGTGAATGCCATCGACTGGCGGCGCAAAACCACCAGCGCAGATGTTTTCATG
TATTATAAACGATTGATCGATCTGCGTAAATCTCATCCGGCCTTCCGCATGGGAGATGCAGGACAGGTTCGAAAACATCT
GGAATTTCTTCCTGTGGAAGGAAGCAACCTGATTGCTTTCCGTCTGAAAGACCATGCAAATGGAGATCATTGGGAAGATA
TCATCGTCGCATTTAATTCTCGTCCTACTCCTGCCAGGCTTACTATTCCGGTTGGTAAATATACAGTGGTATGTAAAGAC
GGAGTGATAGATGTACGTGGATTAGGCATACAAAACGGGCCGGAAGTTATTATTCCCAGTCAGTCGGCTTTGATTTTATA
TAAGTAA

Upstream 100 bases:

>100_bases
GATAAAGTAAAATAAACCCGCAATGAAAGAGGCTGGGAGTGAGAAAAGTAATAAACAAGTAAGTGCGAAGGTAGTGTGAG
TACCGCAGGGGAGGTTTAGT

Downstream 100 bases:

>100_bases
ATTCAGTCTTTTGATAATCAAACAGAATAGAAGAATATGATTCAAAATACATTCAACATAGCGGGGGGCGTAGCACGCAA
CCCGCTTGTGCGTTTAGCTG

Product: pullulanase

Products: NA

Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]

Number of amino acids: Translated: 668; Mature: 668

Protein sequence:

>668_residues
MKMGTNYLAILGVTTVTTVMSCTTAKKEYMSYELYPVRTGSLIEMEYTPEATKFTLWSPTADEVRLMLYEAGEGGHAYET
VKMQSGEEGTWTAVVSKDLIGKFYTFNVKIDDKWQGDTPGINARAVGVNGKRAAIIDWQSTNPDGWESDTRPPLKSPADM
IIYEMHHRDFSVDSTSGVKNKGKYLALTEHGTMNSDKLLTGIDHLIELGVTHVHLLPSFDYASVDETRLNENSYNWGYDP
QNYNVPDGSYATDPYQPATRVKEFKQMVQALHKAGIRVIMDVVYNHTFNTDESNFERTVPGYFYRQKEDKTLANGSGCGN
ETASERLMMRKFMVESVLYWIKEYHVDGFRFDLMGIHDIETMNEIRKAVNAVDPTICIYGEGWAAEAPQYPADSLAMKGN
IAQIPGVAVFSDELRDGLCGPVGDKRKGAFLAGIPGGEMSVKFGIAGAIEHPQVQCDSVNYTQKPWAKQPVQMISYVSCH
DGLCLVDRLKASMPDITPEQLIRLDKLAQTVVFTSQGIPFIYAGEEIMRDKQGVDNSYKSPDAVNAIDWRRKTTSADVFM
YYKRLIDLRKSHPAFRMGDAGQVRKHLEFLPVEGSNLIAFRLKDHANGDHWEDIIVAFNSRPTPARLTIPVGKYTVVCKD
GVIDVRGLGIQNGPEVIIPSQSALILYK

Sequences:

>Translated_668_residues
MKMGTNYLAILGVTTVTTVMSCTTAKKEYMSYELYPVRTGSLIEMEYTPEATKFTLWSPTADEVRLMLYEAGEGGHAYET
VKMQSGEEGTWTAVVSKDLIGKFYTFNVKIDDKWQGDTPGINARAVGVNGKRAAIIDWQSTNPDGWESDTRPPLKSPADM
IIYEMHHRDFSVDSTSGVKNKGKYLALTEHGTMNSDKLLTGIDHLIELGVTHVHLLPSFDYASVDETRLNENSYNWGYDP
QNYNVPDGSYATDPYQPATRVKEFKQMVQALHKAGIRVIMDVVYNHTFNTDESNFERTVPGYFYRQKEDKTLANGSGCGN
ETASERLMMRKFMVESVLYWIKEYHVDGFRFDLMGIHDIETMNEIRKAVNAVDPTICIYGEGWAAEAPQYPADSLAMKGN
IAQIPGVAVFSDELRDGLCGPVGDKRKGAFLAGIPGGEMSVKFGIAGAIEHPQVQCDSVNYTQKPWAKQPVQMISYVSCH
DGLCLVDRLKASMPDITPEQLIRLDKLAQTVVFTSQGIPFIYAGEEIMRDKQGVDNSYKSPDAVNAIDWRRKTTSADVFM
YYKRLIDLRKSHPAFRMGDAGQVRKHLEFLPVEGSNLIAFRLKDHANGDHWEDIIVAFNSRPTPARLTIPVGKYTVVCKD
GVIDVRGLGIQNGPEVIIPSQSALILYK
>Mature_668_residues
MKMGTNYLAILGVTTVTTVMSCTTAKKEYMSYELYPVRTGSLIEMEYTPEATKFTLWSPTADEVRLMLYEAGEGGHAYET
VKMQSGEEGTWTAVVSKDLIGKFYTFNVKIDDKWQGDTPGINARAVGVNGKRAAIIDWQSTNPDGWESDTRPPLKSPADM
IIYEMHHRDFSVDSTSGVKNKGKYLALTEHGTMNSDKLLTGIDHLIELGVTHVHLLPSFDYASVDETRLNENSYNWGYDP
QNYNVPDGSYATDPYQPATRVKEFKQMVQALHKAGIRVIMDVVYNHTFNTDESNFERTVPGYFYRQKEDKTLANGSGCGN
ETASERLMMRKFMVESVLYWIKEYHVDGFRFDLMGIHDIETMNEIRKAVNAVDPTICIYGEGWAAEAPQYPADSLAMKGN
IAQIPGVAVFSDELRDGLCGPVGDKRKGAFLAGIPGGEMSVKFGIAGAIEHPQVQCDSVNYTQKPWAKQPVQMISYVSCH
DGLCLVDRLKASMPDITPEQLIRLDKLAQTVVFTSQGIPFIYAGEEIMRDKQGVDNSYKSPDAVNAIDWRRKTTSADVFM
YYKRLIDLRKSHPAFRMGDAGQVRKHLEFLPVEGSNLIAFRLKDHANGDHWEDIIVAFNSRPTPARLTIPVGKYTVVCKD
GVIDVRGLGIQNGPEVIIPSQSALILYK

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=602, Percent_Identity=24.4186046511628, Blast_Score=122, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- InterPro:   IPR005323
- InterPro:   IPR011840 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48; PF03714 PUD [H]

EC number: =3.2.1.41 [H]

Molecular weight: Translated: 74693; Mature: 74693

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMGTNYLAILGVTTVTTVMSCTTAKKEYMSYELYPVRTGSLIEMEYTPEATKFTLWSPT
CCCCCCEEEEEHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEEECCCCCEEEEECCC
ADEVRLMLYEAGEGGHAYETVKMQSGEEGTWTAVVSKDLIGKFYTFNVKIDDKWQGDTPG
HHHEEEEEEECCCCCCEEEEEEECCCCCCCEEEEEEHHHCCEEEEEEEEECCCCCCCCCC
INARAVGVNGKRAAIIDWQSTNPDGWESDTRPPLKSPADMIIYEMHHRDFSVDSTSGVKN
CCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCC
KGKYLALTEHGTMNSDKLLTGIDHLIELGVTHVHLLPSFDYASVDETRLNENSYNWGYDP
CCCEEEEECCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHCCCCCCCCCCCC
QNYNVPDGSYATDPYQPATRVKEFKQMVQALHKAGIRVIMDVVYNHTFNTDESNFERTVP
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCC
GYFYRQKEDKTLANGSGCGNETASERLMMRKFMVESVLYWIKEYHVDGFRFDLMGIHDIE
CCEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHH
TMNEIRKAVNAVDPTICIYGEGWAAEAPQYPADSLAMKGNIAQIPGVAVFSDELRDGLCG
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHEEECCCCCCCCCEEEEHHHHHCCCCC
PVGDKRKGAFLAGIPGGEMSVKFGIAGAIEHPQVQCDSVNYTQKPWAKQPVQMISYVSCH
CCCCCCCCCEEEECCCCCEEEEEEEEECCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHC
DGLCLVDRLKASMPDITPEQLIRLDKLAQTVVFTSQGIPFIYAGEEIMRDKQGVDNSYKS
CCEEHHHHHHHCCCCCCHHHHHHHHHHHHHEEEECCCCCEEEECHHHHHHHHCCCCCCCC
PDAVNAIDWRRKTTSADVFMYYKRLIDLRKSHPAFRMGDAGQVRKHLEFLPVEGSNLIAF
CCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHEEEECCCCCEEEE
RLKDHANGDHWEDIIVAFNSRPTPARLTIPVGKYTVVCKDGVIDVRGLGIQNGPEVIIPS
EEECCCCCCCCEEEEEEECCCCCCEEEEEECCCEEEEEECCCEEEEECCCCCCCCEEECC
QSALILYK
CCEEEEEC
>Mature Secondary Structure
MKMGTNYLAILGVTTVTTVMSCTTAKKEYMSYELYPVRTGSLIEMEYTPEATKFTLWSPT
CCCCCCEEEEEHHHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEEECCCCCEEEEECCC
ADEVRLMLYEAGEGGHAYETVKMQSGEEGTWTAVVSKDLIGKFYTFNVKIDDKWQGDTPG
HHHEEEEEEECCCCCCEEEEEEECCCCCCCEEEEEEHHHCCEEEEEEEEECCCCCCCCCC
INARAVGVNGKRAAIIDWQSTNPDGWESDTRPPLKSPADMIIYEMHHRDFSVDSTSGVKN
CCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCC
KGKYLALTEHGTMNSDKLLTGIDHLIELGVTHVHLLPSFDYASVDETRLNENSYNWGYDP
CCCEEEEECCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHCCCCCCCCCCCC
QNYNVPDGSYATDPYQPATRVKEFKQMVQALHKAGIRVIMDVVYNHTFNTDESNFERTVP
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCC
GYFYRQKEDKTLANGSGCGNETASERLMMRKFMVESVLYWIKEYHVDGFRFDLMGIHDIE
CCEEECCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHH
TMNEIRKAVNAVDPTICIYGEGWAAEAPQYPADSLAMKGNIAQIPGVAVFSDELRDGLCG
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHEEECCCCCCCCCEEEEHHHHHCCCCC
PVGDKRKGAFLAGIPGGEMSVKFGIAGAIEHPQVQCDSVNYTQKPWAKQPVQMISYVSCH
CCCCCCCCCEEEECCCCCEEEEEEEEECCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHC
DGLCLVDRLKASMPDITPEQLIRLDKLAQTVVFTSQGIPFIYAGEEIMRDKQGVDNSYKS
CCEEHHHHHHHCCCCCCHHHHHHHHHHHHHEEEECCCCCEEEECHHHHHHHHCCCCCCCC
PDAVNAIDWRRKTTSADVFMYYKRLIDLRKSHPAFRMGDAGQVRKHLEFLPVEGSNLIAF
CCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHEEEECCCCCEEEE
RLKDHANGDHWEDIIVAFNSRPTPARLTIPVGKYTVVCKDGVIDVRGLGIQNGPEVIIPS
EEECCCCCCCCEEEEEEECCCCCCEEEEEECCCEEEEEECCCEEEEECCCCCCCCEEECC
QSALILYK
CCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9453151; 10360571 [H]